FastQCFastQC Report
Thu 26 May 2016
SRR936809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465166
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79450.32229066926933114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60110.24383753467312141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.15694683441196253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.051.451591
GTATCAA80150.042.3273471
ATCAACG101600.033.111863
TCAACGC102900.032.601184
TATCAAC104150.032.346772
CAACGCA104700.032.0860865
AACGCAG106750.031.6015226
ACGCAGA121250.027.5866957
CGCAGAG122200.027.1006138
GTACATG86350.024.59641
GCAGAGT135700.023.7387899
TACATGG88100.023.1379742
ACATGGG88050.022.2876683
GAGTACT76950.021.48565112-13
AGAGTAC121950.020.6100110-11
GTACTTT81000.020.174314-15
CAGAGTA130200.020.10689410-11
AGTACTT82250.019.0903312-13
GTGGTAT23700.018.6449381
CATGGGG50450.018.554494
Produced by FastQC (version 0.11.5)