FastQCFastQC Report
Thu 26 May 2016
SRR936809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465166
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91190.36991423701284215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75730.30720040759932593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.19661961912504064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.053.347641
GTATCAA85200.041.0438731
ATCAACG108050.031.8241273
TATCAAC109200.031.6207452
TCAACGC109700.031.3887544
CAACGCA112700.030.5184925
AACGCAG116000.029.7321996
ACGCAGA133900.025.5446747
CGCAGAG134650.025.3318258
GTGGTAT25400.024.878721
TGGTATC24900.024.6065482
GCAGAGT148400.022.5045249
GAGTACT91950.021.95273812-13
GTACATG82150.021.1681821
ACCGATA1604.98741E-520.7838738
GTACTTT98400.020.29655814-15
CAGAGTA142350.020.10703310-11
TACATGG84900.019.6922472
TAGGCCG1456.143552E-419.6576525
AGAGTAC135600.019.46134210-11
Produced by FastQC (version 0.11.5)