FastQCFastQC Report
Thu 26 May 2016
SRR936808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444962
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80380.32875766576331245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61080.24981983360068583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.15435822724443163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46950.044.746181
GTATCAA81850.041.1715161
ATCAACG100800.032.9639553
TCAACGC102750.032.3390244
TATCAAC104600.031.9027462
CAACGCA104350.031.7976115
AACGCAG107400.030.9799586
ACGCAGA121250.027.0834087
CGCAGAG124700.026.2203148
GTACATG85150.025.1186981
TACATGG86450.024.0841732
GCAGAGT133300.023.7790159
ACATGGG87150.022.6907373
GAGTACT75400.021.61893312-13
GTGGTAT26700.020.6498091
CAGAGTA127700.019.85289810-11
AGAGTAC121700.019.77740110-11
AGTACTT80650.019.56343812-13
GTACTTT83050.019.512114-15
CATGGGG49650.019.4360644
Produced by FastQC (version 0.11.5)