##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936808_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2444962 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.605809415442856 31.0 31.0 34.0 30.0 34.0 2 31.69152853909386 33.0 31.0 34.0 30.0 34.0 3 31.71692688884326 33.0 31.0 34.0 30.0 34.0 4 35.31609611928529 37.0 35.0 37.0 33.0 37.0 5 35.31092180573768 37.0 35.0 37.0 33.0 37.0 6 35.37224382219437 37.0 35.0 37.0 33.0 37.0 7 35.30062512219004 37.0 35.0 37.0 33.0 37.0 8 35.26800457430422 37.0 35.0 37.0 32.0 37.0 9 36.87439068582661 39.0 37.0 39.0 33.0 39.0 10-11 36.73392776656652 39.0 37.0 39.0 32.0 39.0 12-13 36.63597757347558 39.0 37.0 39.0 32.0 39.0 14-15 37.76203249784659 40.0 37.5 41.0 32.0 41.0 16-17 37.68108931754359 40.0 37.0 41.0 32.0 41.0 18-19 37.62014092652565 40.0 37.0 41.0 32.0 41.0 20-21 37.54385998637198 40.0 37.0 41.0 32.0 41.0 22-23 37.42651624033421 40.0 37.0 41.0 32.0 41.0 24-25 37.38034865163549 40.0 37.0 41.0 31.5 41.0 26-27 37.404256794175126 40.0 37.0 41.0 32.0 41.0 28-29 37.333709889969654 40.0 37.0 41.0 31.0 41.0 30-31 37.18525420844986 40.0 37.0 41.0 31.0 41.0 32-33 37.01038298345741 39.0 36.0 41.0 30.5 41.0 34-35 36.85299382976095 39.0 36.0 41.0 30.0 41.0 36-37 36.67444995055138 39.0 36.0 41.0 30.0 41.0 38-39 36.51480861461241 39.0 35.0 41.0 30.0 41.0 40-41 36.32685252367931 39.0 35.0 41.0 30.0 41.0 42-43 36.119016573672724 39.0 35.0 40.0 29.5 41.0 44-45 35.88681010175209 39.0 35.0 40.0 28.5 41.0 46-47 35.57794558770239 38.5 35.0 40.0 27.0 41.0 48-49 35.35460939679226 38.5 35.0 40.0 26.0 41.0 50-51 34.67065234551703 38.0 34.0 39.5 25.0 40.5 52-53 34.861338131226574 38.0 34.5 40.0 26.0 40.5 54-55 35.25348042219061 39.0 35.0 40.0 26.5 41.0 56-57 35.12652425681871 38.5 34.5 40.0 26.0 41.0 58-59 34.89810209729231 38.0 34.0 40.0 26.0 41.0 60-61 34.60421245810773 38.0 34.0 40.0 25.5 41.0 62-63 34.27315189356726 37.5 34.0 40.0 25.0 41.0 64-65 33.93351675813366 37.0 33.5 40.0 23.5 41.0 66-67 33.56282203976994 36.5 33.0 39.0 23.0 41.0 68-69 33.16410745852083 36.0 33.0 39.0 22.0 41.0 70-71 32.74973292018444 35.5 32.0 39.0 21.5 40.0 72-73 32.36552347234844 35.0 32.0 38.0 20.5 40.0 74-75 31.958419394657255 35.0 31.5 37.0 20.0 39.5 76-77 31.49469460057048 35.0 31.0 37.0 18.0 39.0 78-79 31.03122686569362 35.0 31.0 36.0 16.0 39.0 80-81 30.598542226832155 34.5 30.5 36.0 12.0 37.5 82-83 30.19762904290537 34.0 30.0 35.0 7.5 37.0 84-85 29.819696993245703 34.0 30.0 35.0 7.0 37.0 86-87 29.45646639906878 34.0 29.5 35.0 2.0 36.0 88-89 29.089212224975277 34.0 29.0 35.0 2.0 36.0 90-91 28.724102051483825 34.0 29.0 35.0 2.0 35.5 92-93 28.32871022126315 34.0 29.0 35.0 2.0 35.0 94-95 27.93564296704816 34.0 28.5 35.0 2.0 35.0 96-97 27.404183991407635 34.0 27.0 35.0 2.0 35.0 98-99 26.791492464913567 33.5 25.5 35.0 2.0 35.0 100-101 24.524006508076607 31.0 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2292701785382789 1101 2 0.21492774700854866 1101 3 0.23865322565156077 1101 4 0.2526288140306434 1101 5 0.2589648744350228 1101 6 0.2274184503945662 1101 7 0.19289808127273034 1101 8 0.29956657252823504 1101 9 0.17182437958651064 1101 10-11 0.2102026804790995 1101 12-13 0.2965349919088851 1101 14-15 0.3954350394423187 1101 16-17 0.4274734612328501 1101 18-19 0.4944040716769109 1101 20-21 0.44384100104803537 1101 22-23 0.5349797840356203 1101 24-25 0.5300070260266594 1101 26-27 0.48739178542212613 1101 28-29 0.4857710167999727 1101 30-31 0.5231502548584501 1101 32-33 0.36667028855285366 1101 34-35 0.3757477147535653 1101 36-37 0.39464140864645003 1101 38-39 0.42342186663852743 1101 40-41 0.45491579827106676 1101 42-43 0.45373573239060505 1101 44-45 0.535336140157419 1101 46-47 0.521178215474599 1101 48-49 0.6117719063072045 1101 50-51 0.6260527216781853 1101 52-53 0.5597674382764737 1101 54-55 0.505794844375508 1101 56-57 0.6420184457797902 1101 58-59 0.6845346752448052 1101 60-61 0.6496394066661253 1101 62-63 0.5959653103815867 1101 64-65 0.6246196729802449 1101 66-67 0.5723119858953183 1101 68-69 0.6136829649111419 1101 70-71 0.609895505829563 1101 72-73 0.4068953230501364 1101 74-75 0.44062084840220805 1101 76-77 0.518817336919497 1101 78-79 0.5457168836453867 1101 80-81 0.6020124531304312 1101 82-83 0.5720282327836372 1101 84-85 0.5441854379860871 1101 86-87 0.7117729293559947 1101 88-89 0.7264217282129195 1101 90-91 0.7821731964236562 1101 92-93 0.6819916305520302 1101 94-95 0.6579566105575871 1101 96-97 0.5908878407568992 1101 98-99 0.39373987247711284 1101 100-101 0.7759776079940615 1102 1 0.16169154656635243 1102 2 0.21035563632293375 1102 3 0.18753616829522102 1102 4 0.21832556620574195 1102 5 0.3224689041733697 1102 6 0.16308283648034916 1102 7 0.11021794475833246 1102 8 0.016782554753589807 1102 9 0.1117214596952536 1102 10-11 0.16741468370503299 1102 12-13 0.0963882496145203 1102 14-15 -8.751729668219355E-4 1102 16-17 0.2039077703557055 1102 18-19 0.07886100501664828 1102 20-21 -0.002435429923004051 1102 22-23 0.0923944758901527 1102 24-25 0.09490530784663775 1102 26-27 0.14658073715606434 1102 28-29 -0.017883921165029903 1102 30-31 0.11094914714773552 1102 32-33 0.060791517182416044 1102 34-35 0.06568551952089052 1102 36-37 0.1233566992202384 1102 38-39 0.18825672480989653 1102 40-41 0.07276735221721964 1102 42-43 0.09701549610746696 1102 44-45 0.10872384424803982 1102 46-47 0.1877701089976469 1102 48-49 0.28269289779909457 1102 50-51 0.13700832245368844 1102 52-53 0.30351506823487284 1102 54-55 0.1204300849580946 1102 56-57 0.1846155039906776 1102 58-59 0.0737694789386012 1102 60-61 0.14687840833783383 1102 62-63 -0.05575567363298006 1102 64-65 0.09621722789844966 1102 66-67 -0.15218583233116334 1102 68-69 0.06215785982816868 1102 70-71 -0.015191417949900199 1102 72-73 -0.1340795585145429 1102 74-75 0.20463694065090365 1102 76-77 0.06482204762755117 1102 78-79 -8.45554600008569E-4 1102 80-81 0.09934745817761481 1102 82-83 0.18439597778585437 1102 84-85 0.002877014791454968 1102 86-87 -0.07836502709399085 1102 88-89 0.016012371956279026 1102 90-91 -0.040320568030256965 1102 92-93 0.23149759371023393 1102 94-95 0.17825271220633354 1102 96-97 -0.03611741472690966 1102 98-99 0.08125369215633782 1102 100-101 -0.02554963346444339 1103 1 0.23264569871288998 1103 2 0.269630937933627 1103 3 0.25798324101235437 1103 4 0.22095899786798867 1103 5 0.11288789132668597 1103 6 0.1657431424691609 1103 7 0.2555420642771722 1103 8 0.2603232535870177 1103 9 0.08818371325262575 1103 10-11 0.21039335583571983 1103 12-13 0.15519312433978882 1103 14-15 0.3937340642312819 1103 16-17 0.46513474107656805 1103 18-19 0.40485194849476613 1103 20-21 0.21212584985009642 1103 22-23 0.3934228289355346 1103 24-25 0.34768219542111467 1103 26-27 0.566018256239353 1103 28-29 0.5502190920399812 1103 30-31 0.43778120657911046 1103 32-33 0.421517023063231 1103 34-35 0.5500551967009315 1103 36-37 0.25225593689778236 1103 38-39 0.21907015894507964 1103 40-41 0.25489617739278714 1103 42-43 0.39327504406363545 1103 44-45 0.3425000782590857 1103 46-47 0.30346471571143496 1103 48-49 0.35178224925724066 1103 50-51 0.4046501192232128 1103 52-53 0.4572619549766159 1103 54-55 0.5642879765473126 1103 56-57 0.549898226952962 1103 58-59 0.5250656289705162 1103 60-61 0.5049285939347072 1103 62-63 0.5264766744766121 1103 64-65 0.5842950574622279 1103 66-67 0.5338398963668354 1103 68-69 0.4921725713139793 1103 70-71 0.47937239785119345 1103 72-73 0.41438393656102335 1103 74-75 0.32769214783555256 1103 76-77 0.3613826385094576 1103 78-79 0.45420006866987706 1103 80-81 0.371674408963532 1103 82-83 0.4617059712502183 1103 84-85 0.3076732619747027 1103 86-87 0.3839805086135364 1103 88-89 0.2520868655707851 1103 90-91 0.3989615594070379 1103 92-93 0.35053458439127994 1103 94-95 0.5869347599038477 1103 96-97 0.4514458913451733 1103 98-99 0.36585199036799665 1103 100-101 0.5544760548589771 1104 1 0.06404025583423234 1104 2 0.10198968202754344 1104 3 0.10195375187541345 1104 4 0.18953271813126094 1104 5 0.19438754573071293 1104 6 0.12213383097886776 1104 7 0.1430258161083131 1104 8 0.04421590219328664 1104 9 0.202252564223393 1104 10-11 0.2042574154020258 1104 12-13 0.13954710488324906 1104 14-15 -0.04434480902268234 1104 16-17 0.23559021851482953 1104 18-19 0.14154218360319248 1104 20-21 0.08173061959033134 1104 22-23 0.10552926217877001 1104 24-25 -0.01802714031247632 1104 26-27 -0.07075554088033442 1104 28-29 0.13911327519552685 1104 30-31 0.2481079495176033 1104 32-33 -0.12712638379092311 1104 34-35 0.09637743657548725 1104 36-37 0.11575777835692236 1104 38-39 0.21621112599482473 1104 40-41 0.22810881434163832 1104 42-43 0.3803100369453887 1104 44-45 0.3475687976970434 1104 46-47 0.2832432892769532 1104 48-49 0.18546876534034595 1104 50-51 0.2534162255429848 1104 52-53 0.2289580961654707 1104 54-55 0.40202789494539815 1104 56-57 0.3775045750867392 1104 58-59 0.3499411886296713 1104 60-61 0.46138459750414995 1104 62-63 0.28568661371361515 1104 64-65 0.2977261678053438 1104 66-67 0.09473545416904727 1104 68-69 0.13297488268047175 1104 70-71 0.03405212838949723 1104 72-73 0.13953184647911598 1104 74-75 0.2018002394236511 1104 76-77 0.37611992109348336 1104 78-79 0.4094842697757848 1104 80-81 0.33623515390339165 1104 82-83 0.3230240459195812 1104 84-85 0.1938828865646265 1104 86-87 0.3545817037566259 1104 88-89 0.2154847058538678 1104 90-91 0.1952552360323736 1104 92-93 0.3627555285388411 1104 94-95 0.26056862418263194 1104 96-97 0.1919269145155802 1104 98-99 0.16927210095870748 1104 100-101 0.28267481638743774 1105 1 0.26892329012903105 1105 2 0.2777718192067411 1105 3 0.18355294560696223 1105 4 0.25706337904427556 1105 5 0.14182253323922822 1105 6 0.131857314967057 1105 7 0.21629972026457267 1105 8 0.2498427475260172 1105 9 0.31697548041690027 1105 10-11 0.25988780945154133 1105 12-13 0.20581229339108376 1105 14-15 0.3665951750790626 1105 16-17 0.38275905226454654 1105 18-19 0.24445709956958694 1105 20-21 0.302242912345271 1105 22-23 0.366802825658219 1105 24-25 0.4196254545092373 1105 26-27 0.4629295885768556 1105 28-29 0.24315952207265923 1105 30-31 0.39552874215898726 1105 32-33 0.35296673453748895 1105 34-35 0.04951166323912304 1105 36-37 0.23257727642642578 1105 38-39 0.49734188911087784 1105 40-41 0.5293018929244226 1105 42-43 0.48013402059290655 1105 44-45 0.20190320037703913 1105 46-47 0.3874840058338478 1105 48-49 0.4732306606390111 1105 50-51 0.18658750080984277 1105 52-53 0.3177618947119143 1105 54-55 0.3949780141822785 1105 56-57 0.32403038135519324 1105 58-59 0.28873434102575857 1105 60-61 0.49663258183301906 1105 62-63 0.382580067040891 1105 64-65 0.42427269857982 1105 66-67 0.4410504666605348 1105 68-69 0.25156093196505225 1105 70-71 0.3520483809445736 1105 72-73 0.42520212810527624 1105 74-75 0.6386394683584662 1105 76-77 0.5586041647909639 1105 78-79 0.5496592924875721 1105 80-81 0.584119408956763 1105 82-83 0.5099299907299368 1105 84-85 0.6215181636484353 1105 86-87 0.7462287341904208 1105 88-89 0.6791224244548175 1105 90-91 0.7247088755963063 1105 92-93 0.6531456034877401 1105 94-95 0.6006520900013967 1105 96-97 0.5708858629598232 1105 98-99 0.49459290495598296 1105 100-101 0.5619368252450379 1106 1 0.3997129160922377 1106 2 0.28441889206888504 1106 3 0.2627705447229509 1106 4 0.2745186473039425 1106 5 0.26365306099693697 1106 6 0.3295845208229693 1106 7 0.2727302042141062 1106 8 0.43116738896920026 1106 9 0.4194002954581393 1106 10-11 0.44756365512907337 1106 12-13 0.442196124906161 1106 14-15 0.4175609164343044 1106 16-17 0.4342187798038637 1106 18-19 0.578677493908522 1106 20-21 0.40398167178936006 1106 22-23 0.37079361940345734 1106 24-25 0.26249861677274566 1106 26-27 0.13911069286972833 1106 28-29 0.14940038855908 1106 30-31 0.31921948261518196 1106 32-33 0.33001860799072347 1106 34-35 0.506642914485532 1106 36-37 0.2172342813205148 1106 38-39 0.08129959787807906 1106 40-41 0.3826461891984394 1106 42-43 0.2570533252852769 1106 44-45 0.0736416982798076 1106 46-47 0.27975562304195734 1106 48-49 0.3790452147600831 1106 50-51 0.44577299572777207 1106 52-53 0.5025321796938798 1106 54-55 0.553272633950229 1106 56-57 0.5487077864729102 1106 58-59 0.6185915721531074 1106 60-61 0.6738467743732031 1106 62-63 0.657680842184007 1106 64-65 0.2567422371882273 1106 66-67 0.3478397526200112 1106 68-69 0.5128008340178241 1106 70-71 0.6512490452595472 1106 72-73 0.5683281681407166 1106 74-75 0.6265871535542438 1106 76-77 0.674250711301184 1106 78-79 0.6712867987890832 1106 80-81 0.7394535443501695 1106 82-83 0.6730466152775527 1106 84-85 0.796110404371074 1106 86-87 0.8126321159197936 1106 88-89 0.9415843783646984 1106 90-91 0.7674430940290868 1106 92-93 0.8347788636984781 1106 94-95 0.9879612874291368 1106 96-97 1.1009294422836717 1106 98-99 1.1689365179532807 1106 100-101 1.6275400539727336 1107 1 0.3778992163463357 1107 2 0.40400243854081097 1107 3 0.3367827540091817 1107 4 0.27110097239221176 1107 5 0.37753258274238277 1107 6 0.3951729369033714 1107 7 0.33814646624924904 1107 8 0.39318523914436554 1107 9 0.48973906352530605 1107 10-11 0.5111756176358995 1107 12-13 0.4963395201252041 1107 14-15 0.4917740037061691 1107 16-17 0.6574113803651471 1107 18-19 0.7892399029730797 1107 20-21 0.6709233930947178 1107 22-23 0.8223837312391211 1107 24-25 0.7501231488000357 1107 26-27 0.7085257445178357 1107 28-29 0.6921004692894712 1107 30-31 0.7778274313542255 1107 32-33 0.715838764650897 1107 34-35 0.6347642019399373 1107 36-37 0.5620044196466694 1107 38-39 0.6587760281312285 1107 40-41 0.8044940353579406 1107 42-43 0.8699737707774844 1107 44-45 0.9438453216295031 1107 46-47 0.8205833389331758 1107 48-49 0.8128731244194043 1107 50-51 0.6917689921982344 1107 52-53 0.7478026833733935 1107 54-55 0.792679567056247 1107 56-57 0.8852878098539989 1107 58-59 0.7461707070828112 1107 60-61 0.8996130589514308 1107 62-63 0.8658706348142147 1107 64-65 0.785507130503909 1107 66-67 0.9821653163960704 1107 68-69 0.8224049062572973 1107 70-71 0.8453587983929225 1107 72-73 0.7007464812358393 1107 74-75 0.887689951891705 1107 76-77 0.8884071204559802 1107 78-79 0.8720767166291346 1107 80-81 0.8879501541668198 1107 82-83 1.010578131033249 1107 84-85 0.9301249343241693 1107 86-87 1.0247396454599524 1107 88-89 0.9329247265329812 1107 90-91 0.9755258591719098 1107 92-93 1.0789659155149458 1107 94-95 1.1495984837753745 1107 96-97 1.0226299716004839 1107 98-99 1.2030017840545177 1107 100-101 1.3413645736383017 1108 1 0.1207704035146051 1108 2 0.1995305178885154 1108 3 0.22434479409111674 1108 4 0.18857739517367378 1108 5 0.028542050727978108 1108 6 0.16146367534265238 1108 7 0.09594834606703984 1108 8 0.09525637755503169 1108 9 -0.007979398384136971 1108 10-11 0.04322380817215077 1108 12-13 0.16608621904963172 1108 14-15 0.31013998785464736 1108 16-17 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-3.920472064812312 2316 90-91 -4.422225192421163 2316 92-93 -4.35795019334461 2316 94-95 -4.439956913390148 2316 96-97 -4.66598852952642 2316 98-99 -4.981601884644885 2316 100-101 -5.3088551169031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5675.0 3 2171.0 4 2213.0 5 2340.0 6 2675.0 7 3062.0 8 3718.0 9 4728.0 10 5622.0 11 7018.0 12 8753.0 13 10390.0 14 11562.0 15 13501.0 16 16186.0 17 20305.0 18 18935.0 19 13719.0 20 13971.0 21 15024.0 22 16719.0 23 18350.0 24 21131.0 25 24176.0 26 28370.0 27 33351.0 28 39787.0 29 47981.0 30 58903.0 31 72076.0 32 91702.0 33 117948.0 34 159966.0 35 225078.0 36 335584.0 37 483519.0 38 420054.0 39 68681.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40598932152965 18.877872718427255 14.31728453102988 23.398853429013215 2 15.325549483145037 20.070894188133078 40.35392270536935 24.249633623352533 3 19.16370946824431 24.395966482266218 29.60572233236428 26.834601717125185 4 12.892643081653556 16.65022320414678 37.125873716663115 33.33125999753655 5 14.124232258529393 36.79521517231554 34.1018867955427 14.978665773612374 6 31.902188935138643 36.001072866909226 17.611847399014355 14.48489079893778 7 28.300388754083883 32.48713804719316 20.909354694413263 18.30311850430969 8 25.931293441051174 35.08084160089869 19.972341831383424 19.015523126666718 9 26.094835013927064 16.989257434759743 20.46302113925631 36.45288641205689 10-11 24.756733038032635 26.68755997890339 27.481609539564637 21.074097443499333 12-13 25.61047164264388 24.019623994860694 28.002340438899832 22.367563923595597 14-15 22.651503043373005 25.375548524419937 26.088939795383993 25.884008636823065 16-17 22.242743199499092 28.644503738382777 26.6338594760944 22.478893586023727 18-19 22.17826644811561 27.22237569908712 28.894454983189167 21.7049028696081 20-21 22.753103226995933 26.50936815329218 28.954932637654444 21.782595982057444 22-23 22.613353583641764 26.342612633475692 28.866059102860053 22.177974680022487 24-25 22.401699827607963 26.600363191099508 28.872997034218866 22.12493994707366 26-27 22.223365200616225 26.763466256721447 28.759556452577723 22.253612090084605 28-29 22.097462228754043 26.73951021419897 28.883545083551383 22.2794824734956 30-31 22.224926329549923 26.646032737104935 28.85576789160684 22.273273041738307 32-33 22.070441560991373 26.82329890982568 28.76387259859477 22.34238693058818 34-35 22.4522965162652 26.812466510416026 28.56822058336299 22.167016389955783 36-37 22.46987115261158 26.634050388345997 28.572743319829115 22.323335139213302 38-39 22.230243546492414 26.822324440706918 28.668187946035623 22.27924406676505 40-41 22.41866627290141 26.68080022153386 28.6734801145137 22.22705339105103 42-43 22.286768041209704 26.779620600107734 28.52051711733305 22.41309424134951 44-45 22.408834866317857 26.947724736840385 28.304833236806026 22.338607160035732 46-47 22.48021510715479 27.03862415186421 28.001109238294408 22.48005150268659 48-49 22.538017563100844 27.13889884703423 27.83711196379648 22.485971626068448 50-51 22.480475256660725 27.306480134957905 27.718724264139734 22.49432034424164 52-53 22.666108749301834 27.2680884969833 27.5837781026842 22.482024651030663 54-55 22.56623240199286 27.236651919380044 27.578237283712205 22.618878394914894 56-57 22.426656009803878 27.618706557365652 27.52569079745266 22.428946635377812 58-59 22.55506840372493 27.283427061999987 27.602079986976563 22.559424547298516 60-61 22.596985934209673 27.162524001458543 27.916909603998686 22.323580460333098 62-63 22.424048438837378 27.265580181062752 27.872885888333386 22.43748549176648 64-65 22.446998789724066 27.281251521025222 27.7450133891121 22.526736300138612 66-67 22.309933553757148 27.57028156810188 27.49220457358956 22.62758030455142 68-69 22.25452212243081 27.392293200161717 27.743861518542705 22.609323158864765 70-71 22.370864792126408 27.346742746197116 27.605234164740395 22.677158296936085 72-73 22.44333886124931 27.320952683043448 27.46046967448285 22.77523878122439 74-75 22.43622967095136 27.47539160915539 27.41294767677073 22.675431043122515 76-77 22.46877932754971 27.451445646206352 27.41033082498832 22.66944420125562 78-79 22.428633877590514 27.46584422935266 27.396539552862105 22.708982340194726 80-81 22.32149307695283 27.603569411905802 27.35780957747032 22.71712793367104 82-83 22.426605434210057 27.547241215307434 27.394473736697027 22.631679613785487 84-85 22.415927234291118 27.52625305358437 27.245315110272646 22.812504601851867 86-87 22.432351267300103 27.443923878119563 27.42389632642741 22.699828528152928 88-89 22.520297473253333 27.430259373482713 27.23498295935022 22.814460193913735 90-91 22.384876587980564 27.4912111606751 27.42930106861402 22.694611182730316 92-93 22.43942808450044 27.3657085980664 27.37186680674375 22.822996510689403 94-95 22.388735867321405 27.298468141277805 27.39134463109053 22.921451360310265 96-97 22.404700299649217 27.384808909706386 27.300150335954836 22.910340454689564 98-99 22.28584580447836 27.275892756677134 27.26208328644933 23.176178152395178 100-101 22.236239423993975 27.400318456998075 27.34033147362087 23.02311064538708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 380.0 1 281.0 2 251.0 3 351.5 4 328.5 5 330.5 6 557.5 7 1138.0 8 1969.5 9 2385.5 10 2187.5 11 2016.5 12 2126.0 13 2283.0 14 2462.0 15 2677.0 16 2816.5 17 2957.0 18 3190.0 19 3422.5 20 3890.0 21 4615.0 22 5608.5 23 6909.0 24 8339.5 25 10514.5 26 13268.0 27 16508.5 28 19812.5 29 23121.0 30 27523.0 31 32610.0 32 37142.0 33 41285.5 34 46904.0 35 51826.0 36 56335.5 37 61614.5 38 65627.0 39 68364.5 40 70787.0 41 75086.0 42 79453.5 43 83887.5 44 89063.0 45 92006.5 46 92185.5 47 92495.0 48 95786.0 49 96640.0 50 94331.5 51 91552.0 52 87720.0 53 85164.0 54 81737.5 55 75949.5 56 70093.0 57 63336.5 58 56949.0 59 50685.0 60 42809.0 61 34395.5 62 27046.0 63 21226.5 64 16135.0 65 12231.5 66 8981.5 67 6731.0 68 4946.5 69 3259.5 70 2184.0 71 1435.5 72 916.5 73 625.0 74 460.5 75 346.0 76 226.5 77 117.5 78 62.5 79 36.5 80 29.0 81 23.0 82 13.5 83 9.5 84 12.0 85 12.5 86 9.0 87 4.5 88 4.5 89 5.5 90 3.0 91 2.5 92 4.5 93 4.0 94 1.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040041522117726165 2 0.03092072596629314 3 0.02633987767499045 4 0.050512032497846596 5 0.09848823826300776 6 0.11889755341800813 7 0.06196415322610331 8 0.09341658479763694 9 0.07677010931049234 10-11 0.07834477591062765 12-13 0.07486823926097828 14-15 0.06963298407091807 16-17 0.05693340019190483 18-19 0.06531798858223564 20-21 0.061248395680587264 22-23 0.03615598115635335 24-25 0.00995925499046611 26-27 0.0033742855717184975 28-29 0.007321177179849829 30-31 0.0049694023874399685 32-33 0.006932623083712549 34-35 0.007034874161643411 36-37 0.006973523514884894 38-39 0.003681038805511088 40-41 0.007730181491573285 42-43 0.004130943548406887 44-45 0.0023722250079960343 46-47 0.001676917678066162 48-49 2.6585280262024524E-4 50-51 0.0021063722053757893 52-53 0.006360017047299713 54-55 0.013558492933632505 56-57 0.008793592702054265 58-59 0.005685159932956013 60-61 0.0023926752235822074 62-63 0.009386648954053273 64-65 0.0026585280262024524 66-67 0.023722250079960344 68-69 0.04899871654446981 70-71 0.06355927004182478 72-73 0.004028692470476024 74-75 0.01509225910259546 76-77 0.024049453529339106 78-79 0.027914544275125748 80-81 0.07879468065352345 82-83 0.029632362384364258 84-85 0.012024726764669553 86-87 0.03390645744187435 88-89 0.05098238745632857 90-91 0.04480642234930441 92-93 0.04374301113882342 94-95 0.012985886897219669 96-97 0.017873488422314947 98-99 0.04036872556710493 100-101 0.07901963302497135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2444962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.633281760020395 #Duplication Level Percentage of deduplicated Percentage of total 1 78.26077861333881 45.886862831944725 2 12.479322231432336 14.634072331393172 3 3.96968780229534 6.982674702338965 4 1.6900719316328285 3.9637785504851824 5 0.9414938419650909 2.7601436855631643 6 0.5694081431228875 2.003176085530457 7 0.38354163121879054 1.5741813170964427 8 0.2785631572833686 1.3066457671165304 9 0.20672119974417144 1.0908668115332514 >10 1.1025404958591005 11.733620869668872 >50 0.08003503045401862 3.196794679304997 >100 0.0367868892504509 3.788082288786052 >500 7.692904287514126E-4 0.2822406842009709 >1k 1.3987098704453487E-4 0.21679594615143769 >5k 1.3987098704453487E-4 0.5800634488856133 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8038 0.32875766576331245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6108 0.24981983360068583 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3774 0.15435822724443163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.090043117234542E-5 0.0 2 0.0 0.0 0.0 4.090043117234542E-5 0.0 3 0.0 0.0 0.0 4.090043117234542E-5 0.0 4 0.0 0.0 0.0 4.090043117234542E-5 4.090043117234542E-5 5 4.090043117234542E-5 0.0 0.0 4.090043117234542E-5 4.090043117234542E-5 6 8.180086234469084E-5 0.0 0.0 4.090043117234542E-5 4.090043117234542E-5 7 2.045021558617271E-4 0.0 0.0 8.180086234469084E-5 4.090043117234542E-5 8 2.045021558617271E-4 0.0 0.0 8.180086234469084E-5 4.090043117234542E-5 9 2.045021558617271E-4 0.0 0.0 8.180086234469084E-5 8.180086234469084E-5 10-11 2.045021558617271E-4 0.0 0.0 8.180086234469084E-5 8.180086234469084E-5 12-13 2.045021558617271E-4 0.0 0.0 8.180086234469084E-5 8.180086234469084E-5 14-15 2.4540258703407254E-4 0.0 0.0 1.4315150910320897E-4 8.180086234469084E-5 16-17 2.4540258703407254E-4 0.0 0.0 1.8405194027555437E-4 8.180086234469084E-5 18-19 2.8630301820641794E-4 0.0 0.0 2.4540258703407254E-4 8.180086234469084E-5 20-21 3.2720344937876334E-4 0.0 0.0 2.8630301820641794E-4 8.180086234469084E-5 22-23 3.2720344937876334E-4 0.0 0.0 4.0900431172345415E-4 1.2270129351703627E-4 24-25 4.090043117234542E-4 0.0 0.0 5.521558208266632E-4 1.6360172468938167E-4 26-27 4.090043117234542E-4 0.0 0.0 8.998094857915993E-4 2.045021558617271E-4 28-29 6.33956683171354E-4 0.0 0.0 0.0020041211274449255 2.045021558617271E-4 30-31 6.544068987575267E-4 0.0 0.0 0.004130943548406887 2.4540258703407254E-4 32-33 6.748571143436994E-4 0.0 0.0 0.006830372005781685 2.8630301820641794E-4 34-35 7.566579766883903E-4 0.0 0.0 0.010081956283983146 2.8630301820641794E-4 36-37 8.180086234469084E-4 0.0 0.0 0.012965436681633496 2.8630301820641794E-4 38-39 8.180086234469084E-4 0.0 0.0 0.01539901233638805 2.8630301820641794E-4 40-41 8.180086234469084E-4 0.0 0.0 0.0203070640770695 2.8630301820641794E-4 42-43 8.793592702054265E-4 0.0 0.0 0.025746821422991444 3.0675323379259064E-4 44-45 9.816103481362901E-4 0.0 0.0 0.031922786530015596 3.2720344937876334E-4 46-47 0.001063411210480981 0.0 0.0 0.039080361985176046 3.2720344937876334E-4 48-49 0.001063411210480981 0.0 0.0 0.044949573858407615 3.6810388055110875E-4 50-51 0.001063411210480981 0.0 0.0 0.05229120125384362 3.6810388055110875E-4 52-53 0.001063411210480981 0.0 0.0 0.07429563322456545 3.6810388055110875E-4 54-55 0.001063411210480981 0.0 0.0 0.1079771382949919 3.6810388055110875E-4 56-57 0.0011043116416533264 4.090043117234542E-5 0.0 0.13243559613605446 3.6810388055110875E-4 58-59 0.0011043116416533264 4.090043117234542E-5 0.0 0.16556494538565425 3.6810388055110875E-4 60-61 0.0011043116416533264 4.090043117234542E-5 0.0 0.21028956687261396 3.6810388055110875E-4 62-63 0.0011043116416533264 4.090043117234542E-5 0.0 0.23525928010333086 3.6810388055110875E-4 64-65 0.0011452120728256718 4.090043117234542E-5 0.0 0.2567933571155707 3.6810388055110875E-4 66-67 0.0012270129351703626 4.090043117234542E-5 0.0 0.3211092851340839 3.6810388055110875E-4 68-69 0.0012270129351703626 4.090043117234542E-5 0.0 0.3924600873142404 3.6810388055110875E-4 70-71 0.001267913366342708 4.090043117234542E-5 0.0 0.4213766921530887 3.6810388055110875E-4 72-73 0.0013088137975150534 4.090043117234542E-5 0.0 0.48201158136609074 4.499047428957996E-4 74-75 0.001329264013101226 4.090043117234542E-5 0.0 0.5671867292825001 4.908051740681451E-4 76-77 0.0013497142286873988 4.090043117234542E-5 0.0 0.6512984659884284 4.908051740681451E-4 78-79 0.0013497142286873988 4.090043117234542E-5 0.0 0.7300113457796071 4.908051740681451E-4 80-81 0.0015951168157214714 4.090043117234542E-5 0.0 0.8376612806252204 5.112553896543178E-4 82-83 0.0016360172468938168 4.090043117234542E-5 0.0 0.9641458640256986 5.317056052404905E-4 84-85 0.0016769176780661622 4.090043117234542E-5 0.0 1.10028294918285 5.317056052404905E-4 86-87 0.0017382683248246805 4.090043117234542E-5 0.0 1.3053781612965762 5.317056052404905E-4 88-89 0.001758718540410853 4.090043117234542E-5 0.0 1.5606786526743566 5.317056052404905E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4695 0.0 44.74618 1 GTATCAA 8185 0.0 41.171516 1 ATCAACG 10080 0.0 32.963955 3 TCAACGC 10275 0.0 32.339024 4 TATCAAC 10460 0.0 31.902746 2 CAACGCA 10435 0.0 31.797611 5 AACGCAG 10740 0.0 30.979958 6 ACGCAGA 12125 0.0 27.083408 7 CGCAGAG 12470 0.0 26.220314 8 GTACATG 8515 0.0 25.118698 1 TACATGG 8645 0.0 24.084173 2 GCAGAGT 13330 0.0 23.779015 9 ACATGGG 8715 0.0 22.690737 3 GAGTACT 7540 0.0 21.618933 12-13 GTGGTAT 2670 0.0 20.649809 1 CAGAGTA 12770 0.0 19.852898 10-11 AGAGTAC 12170 0.0 19.777401 10-11 AGTACTT 8065 0.0 19.563438 12-13 GTACTTT 8305 0.0 19.5121 14-15 CATGGGG 4965 0.0 19.436064 4 >>END_MODULE