FastQCFastQC Report
Thu 26 May 2016
SRR936808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444962
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91740.37522055557509687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75640.3093708613876207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49670.2031524416330397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.050.913431
GTATCAA89000.041.691021
ATCAACG113750.032.1537783
TCAACGC115500.031.6666014
TATCAAC117200.031.451092
CAACGCA117700.030.953635
AACGCAG120950.030.124356
ACGCAGA138200.025.986157
CGCAGAG138750.025.7124828
GTACATG84150.023.6560041
GCAGAGT152200.023.190569
GTGGTAT25950.022.1528451
TACATGG88000.021.969152
ACATGGG86600.021.2817083
GAGTACT92100.021.22389412-13
CGATCGT2850.020.83243624-25
AGAGTAC137200.020.68811410-11
ATTCGCG2900.020.47325780-81
TGGTATC26650.020.319322
GTACTTT97750.020.04450814-15
Produced by FastQC (version 0.11.5)