##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936808_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2444962 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.119916792162826 31.0 31.0 34.0 31.0 34.0 2 32.2525380762564 33.0 31.0 34.0 30.0 34.0 3 32.388991730750824 34.0 31.0 34.0 31.0 34.0 4 36.05785611391915 37.0 35.0 37.0 35.0 37.0 5 35.887949178760245 37.0 35.0 37.0 35.0 37.0 6 35.97465686583268 37.0 35.0 37.0 35.0 37.0 7 35.9309326688922 37.0 35.0 37.0 35.0 37.0 8 35.87401521986845 37.0 35.0 37.0 35.0 37.0 9 37.58176077992214 39.0 37.0 39.0 35.0 39.0 10-11 37.488428041008405 39.0 37.0 39.0 35.0 39.0 12-13 37.378646375690096 39.0 37.0 39.0 34.0 39.0 14-15 38.592871382050106 40.0 38.0 41.0 34.0 41.0 16-17 38.587113214847506 40.0 38.0 41.0 34.0 41.0 18-19 38.69601899743227 40.0 38.0 41.0 34.0 41.0 20-21 38.693196458677065 40.0 38.0 41.0 34.0 41.0 22-23 38.602686667522846 40.0 38.0 41.0 34.0 41.0 24-25 38.512050085032 40.0 38.0 41.0 34.0 41.0 26-27 38.31696832098004 40.0 38.0 41.0 34.0 41.0 28-29 38.16009512622282 40.0 38.0 41.0 33.5 41.0 30-31 38.17223069315597 40.0 38.0 41.0 34.0 41.0 32-33 38.06007250828438 40.0 37.0 41.0 33.5 41.0 34-35 37.95158963615795 40.0 37.0 41.0 33.0 41.0 36-37 37.81403821409086 40.0 37.0 41.0 33.0 41.0 38-39 37.65552695706518 40.0 37.0 41.0 33.0 41.0 40-41 37.33403566190395 40.0 36.5 41.0 32.0 41.0 42-43 37.15265329277101 39.5 36.0 41.0 31.0 41.0 44-45 36.95620606782437 39.0 36.0 41.0 31.0 41.0 46-47 36.62098592943367 39.0 36.0 41.0 30.5 41.0 48-49 36.28286492796207 39.0 35.5 40.0 30.0 41.0 50-51 36.01086642655387 39.0 35.0 40.0 29.5 41.0 52-53 35.74687541156059 38.5 35.0 40.0 28.5 41.0 54-55 35.36085673315168 38.0 34.5 40.0 28.0 41.0 56-57 35.15567154008937 38.0 34.0 40.0 27.5 41.0 58-59 34.824156162754264 38.0 34.0 40.0 26.5 41.0 60-61 34.44250646840319 37.0 34.0 40.0 26.0 41.0 62-63 34.05687307205592 37.0 33.0 39.5 26.0 40.5 64-65 33.728054260148014 36.0 33.0 39.0 26.0 40.0 66-67 33.38158568517629 36.0 32.5 39.0 25.0 40.0 68-69 32.926054065461955 35.5 32.0 38.5 23.5 40.0 70-71 32.48063978090457 35.0 31.5 38.0 22.5 40.0 72-73 32.05406137191498 35.0 31.0 37.0 22.0 39.0 74-75 31.56188439738532 34.5 31.0 37.0 20.5 39.0 76-77 30.143478303548278 33.5 29.5 35.0 20.0 37.5 78-79 30.835509304439086 34.0 30.0 36.0 20.0 38.0 80-81 30.70085854095074 34.0 30.0 35.0 19.0 37.0 82-83 30.34653483367022 34.0 30.0 35.0 18.0 37.0 84-85 29.897458324505656 34.0 30.0 35.0 14.5 36.0 86-87 29.5085126067399 34.0 29.0 35.0 9.0 36.0 88-89 29.123070419908366 34.0 29.0 35.0 7.0 35.5 90-91 28.651692950647085 33.0 29.0 35.0 2.0 35.0 92-93 28.139584991504982 33.0 28.0 35.0 2.0 35.0 94-95 27.55281022772542 33.0 27.0 34.0 2.0 35.0 96-97 26.745795435675483 32.0 25.5 34.0 2.0 35.0 98-99 25.814665217700725 32.0 24.5 34.0 2.0 35.0 100-101 22.482190520752468 28.0 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13353849539874574 1101 2 0.2750289292897605 1101 3 0.12925511584206362 1101 4 0.09540023042303147 1101 5 -0.03315394629702695 1101 6 0.0776147208965483 1101 7 0.0450945241330416 1101 8 0.1437601174063019 1101 9 0.13653410815757638 1101 10-11 0.22476258004076755 1101 12-13 0.22433636321068917 1101 14-15 0.31746558323663976 1101 16-17 0.2276211272415054 1101 18-19 0.10413068197446051 1101 20-21 -0.013909770744938044 1101 22-23 0.10489193767663352 1101 24-25 0.0742310085656186 1101 26-27 0.16721144878081162 1101 28-29 0.13920858479799136 1101 30-31 0.15423087546506764 1101 32-33 0.18981120019923736 1101 34-35 0.049502200527513196 1101 36-37 0.07444153143580223 1101 38-39 0.16801244799086135 1101 40-41 0.21570332332402842 1101 42-43 0.190306345546702 1101 44-45 -0.03008231210194623 1101 46-47 -0.11228437868258112 1101 48-49 -0.05216846037277634 1101 50-51 0.11973022677659628 1101 52-53 0.30323493092038234 1101 54-55 -0.06024273581952855 1101 56-57 0.07922833736029844 1101 58-59 -0.03298450984611634 1101 60-61 0.061717745159022286 1101 62-63 0.22068520391168533 1101 64-65 0.12105182154095218 1101 66-67 0.23616599257940152 1101 68-69 -0.15742285633861286 1101 70-71 -0.1680253817332158 1101 72-73 -0.10208144254106344 1101 74-75 0.02901581941696918 1101 76-77 0.21931257469478993 1101 78-79 0.1299429352554391 1101 80-81 0.14472152909570113 1101 82-83 0.43689441143822094 1101 84-85 0.08726057776890173 1101 86-87 -0.31919346540406934 1101 88-89 -0.030962647094483486 1101 90-91 -0.014200660070883941 1101 92-93 -0.0758537760886675 1101 94-95 -0.09494095304642158 1101 96-97 -0.2892892990476277 1101 98-99 -0.10257288489715322 1101 100-101 0.16866438135535233 1102 1 -0.049009719926544904 1102 2 0.021844928606121528 1102 3 0.08171942501832774 1102 4 0.08710655356050978 1102 5 -0.07237673287845325 1102 6 0.07471752862809211 1102 7 0.09705785324702987 1102 8 0.23043153750852952 1102 9 0.22993137252871065 1102 10-11 0.2929896019866831 1102 12-13 0.21747619433021015 1102 14-15 0.17864029602765186 1102 16-17 0.16785682287208203 1102 18-19 0.15095415860518102 1102 20-21 0.20706316137707148 1102 22-23 0.022793696447791945 1102 24-25 0.06644872659610002 1102 26-27 0.10455849764505132 1102 28-29 -0.041859122283554484 1102 30-31 -0.0052236544522017425 1102 32-33 -0.06762792645044868 1102 34-35 0.1384335053722765 1102 36-37 0.12531504198148724 1102 38-39 0.22255438237208836 1102 40-41 0.23882584980173505 1102 42-43 0.284611435995501 1102 44-45 0.26057918987687145 1102 46-47 0.2388158547164423 1102 48-49 0.20959709377633118 1102 50-51 0.1969469834380817 1102 52-53 0.17936347129520414 1102 54-55 0.4124820242387912 1102 56-57 0.29827038657170846 1102 58-59 0.12778375749991255 1102 60-61 0.12578110471636705 1102 62-63 0.23615843779673895 1102 64-65 0.33235151230059046 1102 66-67 0.3802567778576389 1102 68-69 0.37896749685767617 1102 70-71 0.16911644155648276 1102 72-73 0.0023751703423471326 1102 74-75 0.1552320847120825 1102 76-77 0.2788098321227004 1102 78-79 -0.09944210904764716 1102 80-81 0.20086467693823096 1102 82-83 0.3077805533042408 1102 84-85 0.28132372368868985 1102 86-87 0.15403347642776666 1102 88-89 0.14096577898425267 1102 90-91 0.07929487757472486 1102 92-93 -0.049608536200398845 1102 94-95 0.10364356464009816 1102 96-97 0.3184696163145162 1102 98-99 0.13084229832375271 1102 100-101 0.40276707692718006 1103 1 0.09978863178142916 1103 2 0.20106186372165524 1103 3 0.18937507531394715 1103 4 0.07090511975841451 1103 5 -0.019242782655254587 1103 6 0.04730912213687333 1103 7 0.05087754039454495 1103 8 0.14764536378177695 1103 9 0.19265138242047897 1103 10-11 0.17230797822221433 1103 12-13 0.17710818007797968 1103 14-15 0.24391658429203034 1103 16-17 0.3239980480890239 1103 18-19 0.18208812901566063 1103 20-21 0.19610770967389612 1103 22-23 0.15965404331586086 1103 24-25 0.17681780068091513 1103 26-27 0.12965217927639117 1103 28-29 0.12179808243320167 1103 30-31 0.19153918781695012 1103 32-33 0.1655505115077105 1103 34-35 0.14709732142571852 1103 36-37 0.14121227859416052 1103 38-39 0.13373183115314902 1103 40-41 0.3610809464609943 1103 42-43 0.07194069570242334 1103 44-45 0.20504579127130285 1103 46-47 0.1834083112317657 1103 48-49 0.1844526915850011 1103 50-51 0.08779539204897446 1103 52-53 0.028576356336174058 1103 54-55 0.23459852255189872 1103 56-57 0.025777458430887634 1103 58-59 -0.0510853885021163 1103 60-61 -0.174369984513973 1103 62-63 0.14107807977519116 1103 64-65 0.06293619923361149 1103 66-67 0.16886143254146901 1103 68-69 0.11086095087734549 1103 70-71 -0.01667530386163918 1103 72-73 0.12258119422895675 1103 74-75 -0.08164567369150788 1103 76-77 0.1401754011897225 1103 78-79 0.20565934468939773 1103 80-81 0.05026360045111744 1103 82-83 0.25121482728105704 1103 84-85 -0.0067831251009558 1103 86-87 -0.1823437634148739 1103 88-89 0.07799904258733292 1103 90-91 -0.1342704749836372 1103 92-93 -0.16402358430922703 1103 94-95 -0.027829867627765736 1103 96-97 0.20323660454785042 1103 98-99 -0.019752532721266647 1103 100-101 0.41480185457682595 1104 1 0.0032093871407425922 1104 2 0.12041136957099496 1104 3 0.008906792771185224 1104 4 0.013434314880868214 1104 5 -0.1398016165009679 1104 6 -0.004821193335516227 1104 7 0.04389714651829735 1104 8 0.04621655655211754 1104 9 0.07971448721342966 1104 10-11 0.07774415128842094 1104 12-13 0.11474396834628209 1104 14-15 0.036505033649319785 1104 16-17 0.059641630320243166 1104 18-19 0.14249166389506485 1104 20-21 0.17410717322641034 1104 22-23 0.06082532996450141 1104 24-25 0.09053357636333459 1104 26-27 0.08721163983718583 1104 28-29 0.04859395642994713 1104 30-31 0.11855041452790971 1104 32-33 0.1190746232744786 1104 34-35 0.1318079234848497 1104 36-37 0.11321178341187732 1104 38-39 0.08241605186002943 1104 40-41 0.23791731192614662 1104 42-43 0.14544644445931 1104 44-45 0.22658241670716706 1104 46-47 0.00744683228732157 1104 48-49 0.07226901506282957 1104 50-51 0.11115561653160455 1104 52-53 0.11806295288640456 1104 54-55 0.14734766114103337 1104 56-57 0.007312123770184087 1104 58-59 -0.3234157240912836 1104 60-61 -0.10005337475887899 1104 62-63 0.03412315425885737 1104 64-65 -0.10418440602357038 1104 66-67 -0.14234425895996594 1104 68-69 -0.10986687681985785 1104 70-71 -0.09515170275160045 1104 72-73 -0.09589503702117241 1104 74-75 -0.3109298386903738 1104 76-77 0.06295339962164093 1104 78-79 0.1500910585359314 1104 80-81 0.07551422200805291 1104 82-83 0.11724944877609644 1104 84-85 0.10952410456046735 1104 86-87 -0.05167960016977702 1104 88-89 0.10244615350816488 1104 90-91 0.004108409102443034 1104 92-93 -0.10608203387284831 1104 94-95 0.11034584987073615 1104 96-97 0.17459927141396392 1104 98-99 -0.20965971183282406 1104 100-101 0.4588530904692867 1105 1 -0.001217991918984751 1105 2 0.13110578247128757 1105 3 0.04639401739076732 1105 4 0.058639288762314834 1105 5 -0.006386899262722068 1105 6 0.010009603848224913 1105 7 0.04471902932547778 1105 8 0.08440642709493318 1105 9 0.08217019922796709 1105 10-11 0.09158130777263551 1105 12-13 0.04378240804648925 1105 14-15 0.09906820080807677 1105 16-17 -0.11654222159890537 1105 18-19 -0.0460112034680904 1105 20-21 -0.01608133460914729 1105 22-23 0.04174317007352357 1105 24-25 -0.06432958511746989 1105 26-27 -0.0521329831471391 1105 28-29 -0.017977841208029588 1105 30-31 -0.08758247781822348 1105 32-33 -0.014005804842710745 1105 34-35 0.02904418285346111 1105 36-37 0.009839082980356295 1105 38-39 -0.02724284548269651 1105 40-41 0.022966568115116104 1105 42-43 0.06697650918790998 1105 44-45 -0.001901713431372798 1105 46-47 -0.07625178229364593 1105 48-49 7.737277587196445E-4 1105 50-51 0.08007453545052812 1105 52-53 0.2453859428932219 1105 54-55 0.35072887849283774 1105 56-57 0.15816594502842207 1105 58-59 0.13956051370112732 1105 60-61 0.14437017168673094 1105 62-63 0.2171311885536582 1105 64-65 0.2021599898428832 1105 66-67 0.17581523456717463 1105 68-69 0.11307557718877348 1105 70-71 0.07526974285367061 1105 72-73 0.044903850101242426 1105 74-75 -0.1020232693058638 1105 76-77 -0.07578865125276124 1105 78-79 0.045884409660164494 1105 80-81 -0.10142692607280068 1105 82-83 0.17308637814243966 1105 84-85 -0.24989277029758838 1105 86-87 -0.10068028152340247 1105 88-89 0.09355945693764767 1105 90-91 0.08417668641336817 1105 92-93 0.20518230294296202 1105 94-95 0.0981013574791838 1105 96-97 0.1942598721407407 1105 98-99 0.41349069697934837 1105 100-101 0.6467519662358114 1106 1 -0.007469810448533565 1106 2 0.053245582849868356 1106 3 0.054080063180116156 1106 4 0.12393700452573597 1106 5 0.012555558375758835 1106 6 0.07450340695337587 1106 7 0.09950212983253692 1106 8 0.1653802762313532 1106 9 0.191090066234203 1106 10-11 0.21071845023746505 1106 12-13 0.3024858496034497 1106 14-15 0.2930341154344518 1106 16-17 0.16048774776806596 1106 18-19 0.17622569412866795 1106 20-21 0.13370273033155655 1106 22-23 0.15948891267108678 1106 24-25 0.2262392345792108 1106 26-27 0.2691952217966431 1106 28-29 0.2671906404464792 1106 30-31 0.18309955658100563 1106 32-33 0.07519123058633426 1106 34-35 0.012353759516130935 1106 36-37 0.08455212310406779 1106 38-39 0.14975819087482023 1106 40-41 0.11352263767202686 1106 42-43 0.11120592670268081 1106 44-45 -0.21728541090651987 1106 46-47 -0.1614616201707122 1106 48-49 -0.11287349875895103 1106 50-51 0.06238338960527301 1106 52-53 0.10802394680980143 1106 54-55 0.26042045588128815 1106 56-57 0.280397775261946 1106 58-59 0.155854865389216 1106 60-61 -0.05266068038223182 1106 62-63 -0.05694794077525955 1106 64-65 -0.07545174551652423 1106 66-67 0.014546399504951069 1106 68-69 -0.24124933560353412 1106 70-71 -0.05630205514489006 1106 72-73 0.0821077771442944 1106 74-75 0.1198209893715898 1106 76-77 0.1593338972059435 1106 78-79 0.09749713887662281 1106 80-81 0.255709065610187 1106 82-83 0.3120195837564239 1106 84-85 0.14124261330180943 1106 86-87 0.13283999937198487 1106 88-89 0.37660454993193326 1106 90-91 0.28061433005677117 1106 92-93 0.4572069597516979 1106 94-95 0.6765989006253292 1106 96-97 0.6436401826556839 1106 98-99 0.5393649806013023 1106 100-101 1.0662412087140467 1107 1 0.24512131648928914 1107 2 0.26581487580106966 1107 3 0.20165115540652323 1107 4 0.1692867028580025 1107 5 0.10053385938217474 1107 6 0.06674699506837811 1107 7 0.18832053499594537 1107 8 0.11852366121216562 1107 9 0.1548478477676838 1107 10-11 0.18225075016621162 1107 12-13 0.18848593378449863 1107 14-15 0.3022144953336863 1107 16-17 0.26172238425191097 1107 18-19 0.2719696972714303 1107 20-21 0.2664097996061159 1107 22-23 0.19694644769059266 1107 24-25 0.3013897840501798 1107 26-27 0.37607776230498047 1107 28-29 0.31692732817989366 1107 30-31 0.281912565814757 1107 32-33 0.27222892966788237 1107 34-35 0.27561587770101426 1107 36-37 0.36496489845099944 1107 38-39 0.31095628064746705 1107 40-41 0.4492359185223975 1107 42-43 0.3633527331503359 1107 44-45 0.22149306513581735 1107 46-47 0.18731498644554279 1107 48-49 0.18609949239482404 1107 50-51 0.16431280417155847 1107 52-53 0.2710834047975794 1107 54-55 0.4724013346480618 1107 56-57 0.37255877493849 1107 58-59 0.2948860362680392 1107 60-61 0.2417195138636572 1107 62-63 0.402372228967117 1107 64-65 0.46643420584123163 1107 66-67 0.3855954357266853 1107 68-69 0.2337567599389203 1107 70-71 0.11411035238392486 1107 72-73 0.3504357366676132 1107 74-75 0.08842467976277746 1107 76-77 0.018899139986753966 1107 78-79 0.4261357985322469 1107 80-81 0.28737071617311827 1107 82-83 0.28931288229997776 1107 84-85 0.25486570515054296 1107 86-87 0.5739308948119124 1107 88-89 0.5759483169127115 1107 90-91 0.5786012273896333 1107 92-93 0.6584260303246197 1107 94-95 0.7445259952831336 1107 96-97 0.6584154742273789 1107 98-99 0.8364848510079845 1107 100-101 1.311014683946805 1108 1 0.09209264754549906 1108 2 0.1465680748566598 1108 3 0.029670616051980403 1108 4 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-1.112951637417595 2316 72-73 -1.1808160631089244 2316 74-75 -1.0773382736543802 2316 76-77 -1.0925315047843895 2316 78-79 -1.2628529548059575 2316 80-81 -1.3743561129146897 2316 82-83 -1.3518280809107601 2316 84-85 -1.2588823188038951 2316 86-87 -1.092780420856588 2316 88-89 -1.9103560162685511 2316 90-91 -1.7360437790136451 2316 92-93 -1.4786166780890682 2316 94-95 -1.6562403910168868 2316 96-97 -1.4981064447384362 2316 98-99 -2.0172507782671936 2316 100-101 -2.709297441372442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 18.0 5 53.0 6 101.0 7 255.0 8 604.0 9 1179.0 10 2043.0 11 3284.0 12 4959.0 13 6907.0 14 9127.0 15 12106.0 16 15644.0 17 21267.0 18 19491.0 19 13236.0 20 12201.0 21 12648.0 22 13517.0 23 15251.0 24 17920.0 25 21206.0 26 26152.0 27 33182.0 28 40884.0 29 51388.0 30 64053.0 31 80339.0 32 102914.0 33 133778.0 34 181089.0 35 257538.0 36 389864.0 37 544296.0 38 321294.0 39 15167.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.39328533049915 19.506643446614948 14.971533345687908 23.128537877198 2 15.767573926160553 20.610199634173078 39.593565634519926 24.028660805146444 3 19.421518532258084 24.355437507387666 30.04164088225827 26.181403078095975 4 13.487039880374418 17.18071691911776 36.630753361401936 32.70148983910588 5 14.108948957989078 36.845614195824105 34.18148397799817 14.863952868188646 6 32.151297238975495 36.18064411635027 17.246034907699997 14.422023736974236 7 28.407440945815836 32.46280713206525 20.8050608640312 18.324691058087705 8 26.23333205178649 35.15130296503586 19.66484550680133 18.95051947637632 9 26.034986257443887 16.745550029448335 20.39837052548917 36.82109318761861 10-11 24.889037130229426 26.716959200183886 27.356335190485577 21.037668479101107 12-13 25.709204990970687 23.899947439785834 28.098915459860972 22.291932109382508 14-15 22.70007769292704 25.27300402902963 26.215156031079623 25.81176224696371 16-17 22.230263701439938 28.579380783832224 26.839128788095685 22.351226726632152 18-19 22.10591433967396 26.93374806616502 29.30048600225567 21.659851591905348 20-21 22.652043750136762 26.194252226904702 29.55584199871612 21.597862024242417 22-23 22.496716154922638 26.07457861155983 29.445788385607973 21.982916847909557 24-25 22.36396609817329 26.316718351600976 29.463357412977487 21.855958137248248 26-27 22.31527102662689 26.48596384348414 29.260740541691284 21.938024588197692 28-29 22.056220937675487 26.38909168694306 29.48421263161253 22.07047474376892 30-31 22.22713481845526 26.328343753399846 29.392358654244934 22.052162773899965 32-33 22.15852190356839 26.429029076398326 29.333949022794076 22.078499997239216 34-35 22.459203864927144 26.456893808574527 29.13049364366399 21.953408682834336 36-37 22.459844710316045 26.294866343996897 29.11157015920189 22.133718786485172 38-39 22.257868502856894 26.435334894943118 29.273741005796417 22.033055596403567 40-41 22.472937919796607 26.30821527288671 29.191953647828527 22.02689315948815 42-43 22.33280108238901 26.429531420120234 28.99239333781057 22.245274159680193 44-45 22.43809454472874 26.592146712922784 28.8155568771069 22.15420186524157 46-47 22.5164333189568 26.741764117275707 28.4138797561429 22.327922807624596 48-49 22.632627076877014 26.915790339052997 28.152928660913055 22.29865392315693 50-51 22.64860151300979 26.96193916548872 28.06096948458196 22.328489836919534 52-53 22.809214337877187 26.986640424826597 27.90590238943488 22.29824284786134 54-55 22.635259770908505 26.956635726853833 27.911722145374856 22.496382356862807 56-57 22.532245979068744 27.28417198509912 27.854243593749743 22.329338442082395 58-59 22.67731742686021 26.98655212348185 27.947325077296814 22.388805372361126 60-61 22.66056445523877 26.832279032404514 28.290310547726577 22.21684596463013 62-63 22.490804047425694 26.96668229745131 28.253798679447506 22.288714975675486 64-65 22.582709975913705 27.000943574104923 28.01010160088574 22.40624484909563 66-67 22.432045978628707 27.23230463295544 27.84773342080572 22.48791596761013 68-69 22.387646423663373 27.028560794450623 28.10313870165565 22.48065408023035 70-71 22.470570503692606 27.009491182552143 27.932187667372816 22.58775064638244 72-73 22.51263620888375 26.9867621447894 27.794833285479754 22.7057683608471 74-75 22.570719280230996 27.15261245317029 27.675947704104324 22.600720562494388 76-77 22.52588078092043 27.153377906641456 27.735861635420772 22.584879677017344 78-79 22.54889450043723 27.14263394299616 27.624871265682273 22.683600290884343 80-81 22.448834768552945 27.293283793920626 27.671639422443743 22.58624201508269 82-83 22.546258425495715 27.270302990641976 27.647916530331784 22.535522053530528 84-85 22.45170578876114 27.25200518628498 27.56344814328544 22.732840881668444 86-87 22.495523950825323 27.081268283804523 27.727332683696954 22.695875081673197 88-89 22.597323663171103 27.200296616356685 27.482904271692732 22.719475448779484 90-91 22.465668821461513 27.21419996850632 27.681513005458225 22.638618204573945 92-93 22.42026665445107 26.94992805614157 27.727547503805784 22.902257785601577 94-95 22.374974002658117 27.012777092809024 27.730211702720066 22.88203720181279 96-97 22.488745718996867 26.890620024838867 27.64783124130737 22.9728030148569 98-99 22.198645289823805 26.893626907042307 27.56650337777731 23.341224425356582 100-101 22.071423268852943 27.160724339426896 27.58131353650563 23.18653885521453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 517.0 1 394.0 2 310.0 3 369.0 4 344.5 5 345.0 6 573.5 7 1262.0 8 2139.5 9 2607.0 10 2448.0 11 2184.5 12 2249.0 13 2428.5 14 2652.5 15 2935.0 16 3129.5 17 3251.0 18 3519.5 19 3854.0 20 4313.0 21 5009.5 22 5962.5 23 7088.5 24 8548.0 25 10857.0 26 13426.0 27 16603.5 28 19761.5 29 22994.0 30 27572.0 31 32196.5 32 36513.0 33 40734.0 34 46312.0 35 51284.5 36 55805.5 37 61150.5 38 65483.5 39 68231.5 40 70424.5 41 74795.5 42 79351.5 43 84003.0 44 89074.5 45 91706.0 46 92018.0 47 92860.0 48 96427.5 49 96659.5 50 94064.5 51 91322.5 52 87354.0 53 85073.5 54 82001.5 55 76418.0 56 70000.0 57 63215.5 58 57088.5 59 50745.0 60 43008.5 61 34575.5 62 27030.5 63 21115.5 64 15833.0 65 11864.5 66 8900.5 67 6730.0 68 4817.5 69 3069.5 70 2033.5 71 1369.0 72 937.5 73 666.5 74 492.5 75 356.5 76 218.0 77 108.5 78 57.0 79 31.0 80 13.5 81 6.5 82 5.0 83 5.5 84 5.0 85 2.5 86 1.0 87 0.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021390925503136655 2 0.003681038805511088 3 3.681038805511088E-4 4 0.0 5 0.0030266319067535612 6 0.0 7 1.6360172468938167E-4 8 0.0 9 8.180086234469084E-5 10-11 0.0 12-13 0.006012363382334776 14-15 0.0032924847093738063 16-17 0.0 18-19 5.930562519990086E-4 20-21 0.0013292640131012262 22-23 0.0015746666001352986 24-25 8.180086234469084E-4 26-27 3.476536649649361E-4 28-29 4.090043117234542E-5 30-31 0.0 32-33 2.6585280262024524E-4 34-35 0.0 36-37 0.0019427704806864075 38-39 0.010736363182740672 40-41 0.0011452120728256718 42-43 0.0 44-45 9.816103481362901E-4 46-47 2.249523714478998E-4 48-49 2.045021558617271E-4 50-51 0.0013906146598597444 52-53 4.703549584819723E-4 54-55 0.0 56-57 2.4540258703407254E-4 58-59 1.4315150910320897E-4 60-61 0.0022904241456513435 62-63 2.045021558617271E-5 64-65 1.2270129351703627E-4 66-67 0.0 68-69 8.180086234469084E-5 70-71 3.476536649649361E-4 72-73 1.6360172468938167E-4 74-75 0.0027198786729609706 76-77 4.090043117234542E-5 78-79 1.6360172468938167E-4 80-81 0.0016360172468938168 82-83 8.180086234469084E-5 84-85 0.002126822420961962 86-87 1.840519402755544E-4 88-89 0.002167722852134307 90-91 0.0011043116416533264 92-93 0.0 94-95 2.045021558617271E-5 96-97 1.4315150910320897E-4 98-99 4.499047428957996E-4 100-101 0.0023926752235822074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2444962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.37541932287215 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76980689930056 43.27930055283993 2 13.169899387820532 14.849172008568395 3 4.223131935001019 7.142425010744848 4 1.917632499866034 4.32429345148461 5 1.0053685718716312 2.8339037406650163 6 0.6279510584997755 2.124060254229976 7 0.4194794314124062 1.6553830190236072 8 0.29730455001125394 1.3408534938785803 9 0.22106770242025564 1.1216505980417246 >10 1.2170604534793616 12.361813113768 >50 0.08944675120899655 3.4573125812140115 >100 0.040613324189779955 4.135090807040076 >500 8.734834717457463E-4 0.34552169555518303 >1k 2.1837086793598614E-4 0.3423619365810609 >5k 1.4558057862399076E-4 0.6868577363650721 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9174 0.37522055557509687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7564 0.3093708613876207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4967 0.2031524416330397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.090043117234542E-5 0.0 0.0 0.0 0.0 6 4.090043117234542E-5 0.0 0.0 0.0 8.180086234469084E-5 7 4.090043117234542E-5 0.0 0.0 4.090043117234542E-5 8.180086234469084E-5 8 8.180086234469084E-5 0.0 0.0 4.090043117234542E-5 8.180086234469084E-5 9 8.180086234469084E-5 0.0 0.0 4.090043117234542E-5 8.180086234469084E-5 10-11 8.180086234469084E-5 0.0 0.0 4.090043117234542E-5 8.180086234469084E-5 12-13 8.180086234469084E-5 0.0 0.0 4.090043117234542E-5 8.180086234469084E-5 14-15 8.180086234469084E-5 0.0 0.0 8.180086234469084E-5 1.2270129351703627E-4 16-17 1.4315150910320897E-4 0.0 0.0 1.0225107793086355E-4 1.2270129351703627E-4 18-19 2.2495237144789983E-4 0.0 0.0 1.2270129351703627E-4 1.2270129351703627E-4 20-21 2.4540258703407254E-4 0.0 0.0 1.2270129351703627E-4 1.4315150910320897E-4 22-23 2.4540258703407254E-4 0.0 0.0 2.4540258703407254E-4 2.045021558617271E-4 24-25 2.8630301820641794E-4 0.0 0.0 4.294545273096269E-4 2.4540258703407254E-4 26-27 2.8630301820641794E-4 0.0 0.0 8.589090546192538E-4 2.4540258703407254E-4 28-29 4.7035495848197237E-4 0.0 0.0 0.002045021558617271 2.4540258703407254E-4 30-31 4.908051740681451E-4 0.0 0.0 0.004212744410751578 2.4540258703407254E-4 32-33 4.908051740681451E-4 0.0 0.0 0.006953073299298722 2.8630301820641794E-4 34-35 5.112553896543178E-4 0.0 0.0 0.01012285671515549 2.8630301820641794E-4 36-37 5.317056052404905E-4 0.0 0.0 0.013026787328392016 2.8630301820641794E-4 38-39 5.726060364128359E-4 0.0 0.0 0.015439912767560394 2.8630301820641794E-4 40-41 5.930562519990086E-4 0.0 0.0 0.02036841472382802 2.8630301820641794E-4 42-43 6.953073299298721E-4 0.0 0.0 0.02558321969830206 2.8630301820641794E-4 44-45 6.953073299298721E-4 0.0 0.0 0.031738734589740046 3.2720344937876334E-4 46-47 7.771081922745629E-4 4.090043117234542E-5 0.0 0.038937210476072834 5.112553896543178E-4 48-49 8.180086234469084E-4 4.090043117234542E-5 0.0 0.044724621486959715 5.317056052404905E-4 50-51 8.180086234469084E-4 4.090043117234542E-5 0.0 0.05202534845122338 5.317056052404905E-4 52-53 8.180086234469084E-4 4.090043117234542E-5 0.0 0.07398887999077286 5.521558208266632E-4 54-55 8.180086234469084E-4 4.090043117234542E-5 0.0 0.10760903441444081 5.726060364128359E-4 56-57 8.998094857915992E-4 4.090043117234542E-5 0.0 0.13223109398019273 5.726060364128359E-4 58-59 8.998094857915992E-4 4.090043117234542E-5 0.0 0.16548314452330956 5.726060364128359E-4 60-61 8.998094857915992E-4 4.090043117234542E-5 0.0 0.21018731579468308 5.726060364128359E-4 62-63 8.998094857915992E-4 4.090043117234542E-5 0.0 0.2353001805345032 5.726060364128359E-4 64-65 8.998094857915992E-4 4.090043117234542E-5 0.0 0.25691605840908777 5.726060364128359E-4 66-67 9.407099169639446E-4 4.090043117234542E-5 0.0 0.32127288685877325 5.726060364128359E-4 68-69 9.816103481362901E-4 4.090043117234542E-5 0.0 0.39323719550651504 5.726060364128359E-4 70-71 0.0010020605637224627 4.090043117234542E-5 0.0 0.4217856964648121 5.726060364128359E-4 72-73 0.0010225107793086355 4.090043117234542E-5 0.0 0.48217518309078017 5.726060364128359E-4 74-75 0.0010225107793086355 4.090043117234542E-5 0.0 0.5673298807916033 5.726060364128359E-4 76-77 0.0010225107793086355 4.090043117234542E-5 0.0 0.6522596261209785 6.135064675851813E-4 78-79 0.0010225107793086355 4.090043117234542E-5 0.0 0.7311770080680191 6.135064675851813E-4 80-81 0.0011452120728256718 4.090043117234542E-5 0.0 0.8389291939915631 6.135064675851813E-4 82-83 0.0011861125039980172 4.090043117234542E-5 0.0 0.9657000804102477 6.135064675851813E-4 84-85 0.0011861125039980172 4.090043117234542E-5 0.0 1.1011214080218834 6.135064675851813E-4 86-87 0.0012474631507565352 4.090043117234542E-5 0.0 1.3050509578471976 6.135064675851813E-4 88-89 0.0013088137975150534 4.090043117234542E-5 0.0 1.5586540813313254 6.135064675851813E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 50.91343 1 GTATCAA 8900 0.0 41.69102 1 ATCAACG 11375 0.0 32.153778 3 TCAACGC 11550 0.0 31.666601 4 TATCAAC 11720 0.0 31.45109 2 CAACGCA 11770 0.0 30.95363 5 AACGCAG 12095 0.0 30.12435 6 ACGCAGA 13820 0.0 25.98615 7 CGCAGAG 13875 0.0 25.712482 8 GTACATG 8415 0.0 23.656004 1 GCAGAGT 15220 0.0 23.19056 9 GTGGTAT 2595 0.0 22.152845 1 TACATGG 8800 0.0 21.96915 2 ACATGGG 8660 0.0 21.281708 3 GAGTACT 9210 0.0 21.223894 12-13 CGATCGT 285 0.0 20.832436 24-25 AGAGTAC 13720 0.0 20.688114 10-11 ATTCGCG 290 0.0 20.473257 80-81 TGGTATC 2665 0.0 20.31932 2 GTACTTT 9775 0.0 20.044508 14-15 >>END_MODULE