FastQCFastQC Report
Thu 26 May 2016
SRR936807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489701
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166170.6674295427442893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112020.44993354623707826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81660.3279911925166918No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.10527368547468151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72200.043.124741
GTATCAA130100.041.7629661
ATCAACG162650.033.021983
TCAACGC164700.032.6398164
TATCAAC167650.032.211132
CAACGCA167650.032.033915
AACGCAG172900.031.086836
ACGCAGA198450.026.9157547
CGCAGAG199300.026.6361948
GTACATG134600.025.7103961
TACATGG135000.025.0713312
GCAGAGT209300.024.9094269
CATTCCG3900.024.3720289
ACATGGG134050.024.1113133
GTCTACG1852.525594E-723.125611
GAGTACT110550.022.4595712-13
AGTACTT118550.021.6253812-13
AGAGTAC185000.021.44895410-11
CATGGGA88550.021.0951544
CATGGGG47850.021.0587524
Produced by FastQC (version 0.11.5)