FastQCFastQC Report
Thu 26 May 2016
SRR936807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489701
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150890.6060567112275731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125350.5034741119515957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.35899893200026833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.12226367744560493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.042.3136521
GTATCAA152200.035.9862251
TATCAAC198750.027.5305352
ATCAACG199150.027.4502853
TCAACGC201200.027.1942024
CAACGCA204950.026.6734525
AACGCAG210350.025.9897486
ACGCAGA238850.022.7096337
CGCAGAG239200.022.4584338
GAGTACT138250.022.43454712-13
GTACATG123850.022.1693741
AGTACTT144150.021.89523512-13
TACACCG1553.911676E-521.449555
GCAGAGT248250.021.1613739
TACATGG127250.020.827912
GTACTTT148600.020.79269814-15
AGAGTAC212750.020.72957210-11
ACATGGG126700.020.4676463
TACTTTT168050.018.4850914-15
CAGAGTA243750.018.47318310-11
Produced by FastQC (version 0.11.5)