##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936807_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2489701 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38586239873784 31.0 31.0 34.0 30.0 34.0 2 31.38509644330785 31.0 31.0 34.0 30.0 34.0 3 31.548025646453127 31.0 31.0 34.0 30.0 34.0 4 35.42627287373062 37.0 35.0 37.0 33.0 37.0 5 35.31674325551542 37.0 35.0 37.0 33.0 37.0 6 35.3824889012777 37.0 35.0 37.0 33.0 37.0 7 35.298331807715066 37.0 35.0 37.0 32.0 37.0 8 35.27569173969083 37.0 35.0 37.0 32.0 37.0 9 36.812310393898706 39.0 37.0 39.0 33.0 39.0 10-11 36.68551524861821 38.0 35.0 39.0 32.0 39.0 12-13 36.54902677068451 38.0 35.0 39.0 32.0 39.0 14-15 37.474265584501914 39.0 36.0 40.0 32.0 41.0 16-17 37.34649401675141 39.0 36.0 40.0 32.0 41.0 18-19 37.437140845426825 39.0 36.0 40.0 32.0 41.0 20-21 37.56470114282799 39.0 36.0 41.0 32.0 41.0 22-23 37.46887176411947 39.0 36.0 41.0 32.0 41.0 24-25 37.32482635465062 39.0 36.0 40.5 32.0 41.0 26-27 37.07903278345472 39.0 36.0 40.0 31.0 41.0 28-29 36.84003079084597 39.0 36.0 40.0 30.5 41.0 30-31 36.6130549411355 38.0 35.0 40.0 30.0 41.0 32-33 36.516359594987506 38.0 35.0 40.0 30.0 41.0 34-35 36.36343882257347 38.0 35.0 40.0 30.0 41.0 36-37 36.134750116580264 38.0 35.0 40.0 30.0 41.0 38-39 35.91659018492582 38.0 35.0 40.0 29.5 41.0 40-41 35.653367211564756 38.0 34.5 40.0 28.5 41.0 42-43 35.33260499955617 38.0 34.0 40.0 27.0 41.0 44-45 34.893293210710844 38.0 33.5 40.0 26.0 41.0 46-47 34.50666626233431 38.0 33.0 40.0 24.5 41.0 48-49 34.0736540251219 38.0 33.0 40.0 23.5 41.0 50-51 33.738819641394684 37.5 33.0 40.0 22.5 41.0 52-53 33.41115077673986 37.0 32.5 40.0 21.5 41.0 54-55 33.119188810222596 37.0 32.0 40.0 20.0 41.0 56-57 32.77655589968434 37.0 31.0 39.0 18.5 40.0 58-59 32.34731379390537 36.0 31.0 39.0 17.0 40.0 60-61 31.92991427484666 36.0 30.5 39.0 13.5 40.0 62-63 31.463781193002696 35.0 29.5 38.5 11.5 40.0 64-65 30.987385834684567 35.0 29.0 38.0 8.0 40.0 66-67 30.52949852211169 34.0 28.5 38.0 8.0 40.0 68-69 29.99050689219308 34.0 28.0 37.5 7.0 39.0 70-71 29.409243921258014 34.0 27.5 37.0 4.0 39.0 72-73 28.92679241402883 33.5 26.0 36.0 2.0 39.0 74-75 28.360126778275784 33.0 26.0 36.0 2.0 38.5 76-77 27.291326548850645 31.5 25.5 34.5 2.0 36.5 78-79 27.988743226596284 32.5 26.0 35.0 2.0 37.0 80-81 27.734669544656164 33.0 26.0 35.0 2.0 37.0 82-83 27.2645940215311 32.0 26.0 35.0 2.0 36.0 84-85 26.71906144553101 32.0 25.0 35.0 2.0 36.0 86-87 26.180294340565393 32.0 24.0 34.0 2.0 35.5 88-89 25.598076234857118 31.0 22.0 34.0 2.0 35.0 90-91 24.89432767227872 31.0 19.5 34.0 2.0 35.0 92-93 24.10766292819901 31.0 15.0 34.0 2.0 35.0 94-95 23.272042707136322 30.0 4.5 34.0 2.0 35.0 96-97 22.206438242985804 29.5 2.0 34.0 2.0 35.0 98-99 20.955009858613543 29.0 2.0 34.0 2.0 35.0 100-101 17.77085923169087 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3826002879370982 1101 2 0.20429037118196902 1101 3 0.2840412989596892 1101 4 0.27073486336173147 1101 5 0.3591806819888461 1101 6 0.2446077582985282 1101 7 0.29324721839880397 1101 8 0.33736292062388884 1101 9 0.42207757706741234 1101 10-11 0.41676426518723275 1101 12-13 0.47859474995818374 1101 14-15 0.5366493191049955 1101 16-17 0.6021438649161865 1101 18-19 0.45920473996182665 1101 20-21 0.5207064942744708 1101 22-23 0.47930255941759015 1101 24-25 0.4766488310065 1101 26-27 0.45871550469214384 1101 28-29 0.5526151138831921 1101 30-31 0.6082002060509524 1101 32-33 0.7223391785807038 1101 34-35 0.6966961762734556 1101 36-37 0.8441141786254533 1101 38-39 0.7608234596547518 1101 40-41 0.7671291652284324 1101 42-43 0.867648974091594 1101 44-45 1.0117823853769252 1101 46-47 1.1417156064079634 1101 48-49 0.9531314621170779 1101 50-51 0.8134480159101116 1101 52-53 0.7441250640142627 1101 54-55 0.9595346206513042 1101 56-57 1.0300516108639641 1101 58-59 0.88701758836865 1101 60-61 1.0288158185673382 1101 62-63 1.0376746936072259 1101 64-65 1.0107672631860183 1101 66-67 0.9998850231078933 1101 68-69 0.8870474804800388 1101 70-71 0.8616742560086976 1101 72-73 1.1046390686746719 1101 74-75 0.9281376503114522 1101 76-77 0.6966812358167296 1101 78-79 0.9633604291548785 1101 80-81 1.2980458386077949 1101 82-83 1.5316633218398472 1101 84-85 1.2380800790484905 1101 86-87 1.291316262104914 1101 88-89 1.4351840896734842 1101 90-91 1.607448726893466 1101 92-93 1.6532077563316747 1101 94-95 1.856256483528135 1101 96-97 1.5471606817046073 1101 98-99 1.449771859596492 1101 100-101 1.3672490713921697 1102 1 0.23635856124306187 1102 2 0.12293310543414648 1102 3 0.22242253057153505 1102 4 0.26339414495299707 1102 5 0.3011406501681577 1102 6 0.2378142306379445 1102 7 0.314726909441454 1102 8 0.3295702699228329 1102 9 0.4443527852907607 1102 10-11 0.43825868129653145 1102 12-13 0.4104551403287857 1102 14-15 0.5433226829501194 1102 16-17 0.539669951916963 1102 18-19 0.558500409119759 1102 20-21 0.5330286481917668 1102 22-23 0.4621665812441407 1102 24-25 0.46170343373511713 1102 26-27 0.5314858750948304 1102 28-29 0.494626165318401 1102 30-31 0.3025967182825795 1102 32-33 0.4377345448022041 1102 34-35 0.47190112738623213 1102 36-37 0.6583141966591128 1102 38-39 0.655255457479079 1102 40-41 0.6503821106053493 1102 42-43 0.6845364076833604 1102 44-45 0.7688993191308526 1102 46-47 0.7661395108993574 1102 48-49 0.7957172274496855 1102 50-51 0.9962102711649621 1102 52-53 0.9523287804029295 1102 54-55 0.8480553222770979 1102 56-57 0.9065472049785868 1102 58-59 1.0518808888487214 1102 60-61 0.9534998295446115 1102 62-63 1.274380867082325 1102 64-65 1.1713437513630645 1102 66-67 1.0722556352198254 1102 68-69 1.0473051073373973 1102 70-71 0.9202409362006101 1102 72-73 1.1578827281700477 1102 74-75 0.7325782213967997 1102 76-77 0.9659116274816917 1102 78-79 1.3665165744887382 1102 80-81 1.340642365964424 1102 82-83 1.4703535980602815 1102 84-85 1.2526189917641126 1102 86-87 1.2151187687839133 1102 88-89 1.463382592297819 1102 90-91 1.531629722322517 1102 92-93 1.3471928756519809 1102 94-95 1.5317252860202721 1102 96-97 1.615521894497455 1102 98-99 1.8771920061054495 1102 100-101 1.820724223478475 1103 1 0.17330915503188749 1103 2 0.007306488736784189 1103 3 0.1505591476914887 1103 4 0.24582374107497884 1103 5 0.2741003502560062 1103 6 0.32784084906901256 1103 7 0.3756912915029247 1103 8 0.5010480353037963 1103 9 0.32438407389660995 1103 10-11 0.40121368472240704 1103 12-13 0.4913832854940452 1103 14-15 0.36932014571725347 1103 16-17 0.5871143646169088 1103 18-19 0.5525465081633385 1103 20-21 0.5287912381293509 1103 22-23 0.6538703931975505 1103 24-25 0.6170113660853573 1103 26-27 0.7241139097001152 1103 28-29 0.7500384331535841 1103 30-31 0.7725113536519714 1103 32-33 0.8162720155805019 1103 34-35 0.8205282296421146 1103 36-37 0.7159386633182905 1103 38-39 0.787296010825564 1103 40-41 0.7204931244763344 1103 42-43 0.856815651627933 1103 44-45 1.0102538070676843 1103 46-47 1.0150513987034415 1103 48-49 0.9236031432307499 1103 50-51 0.7966806356613247 1103 52-53 0.883852568496188 1103 54-55 0.9725318539565677 1103 56-57 0.9107574054638121 1103 58-59 1.0348997649511134 1103 60-61 1.0056853906741168 1103 62-63 1.0108521388856566 1103 64-65 0.8348096206205646 1103 66-67 0.9839320834887033 1103 68-69 0.9477665143446359 1103 70-71 0.7498869504369985 1103 72-73 1.0128081853661932 1103 74-75 0.9723063351145349 1103 76-77 1.0050344244239575 1103 78-79 1.0176930455727238 1103 80-81 1.3150551899103924 1103 82-83 1.498916008944402 1103 84-85 1.360265376580493 1103 86-87 1.1160984821177742 1103 88-89 1.4423778220736665 1103 90-91 1.9111475204561081 1103 92-93 1.8135744798588185 1103 94-95 1.753926157954787 1103 96-97 2.02435072807706 1103 98-99 2.1347415501102596 1103 100-101 1.6664182296300076 1104 1 0.27504799412423964 1104 2 0.1573595301495061 1104 3 0.21007790946623217 1104 4 0.18064423017977305 1104 5 0.20438906090669207 1104 6 0.19128801473282664 1104 7 0.24235852499616328 1104 8 0.29692294259376695 1104 9 0.2776889007822234 1104 10-11 0.2880497783163136 1104 12-13 0.3566589597496588 1104 14-15 0.45455681491122135 1104 16-17 0.3474908951872777 1104 18-19 0.5161478013658538 1104 20-21 0.36239202734910236 1104 22-23 0.36388740169144285 1104 24-25 0.35741015568882517 1104 26-27 0.2697736385327474 1104 28-29 0.26290206934358906 1104 30-31 0.49272569542399225 1104 32-33 0.45995842395415565 1104 34-35 0.3711858901738694 1104 36-37 0.49232767491692186 1104 38-39 0.48822340071118475 1104 40-41 0.6753165536859171 1104 42-43 0.7423652337350504 1104 44-45 0.7399001430206127 1104 46-47 0.7381363474000935 1104 48-49 0.6885440447831996 1104 50-51 0.5467181263102248 1104 52-53 0.5419977369712186 1104 54-55 0.5550276702219605 1104 56-57 0.3849812134860571 1104 58-59 0.35720345411722576 1104 60-61 0.5545478128694654 1104 62-63 0.5875893668350862 1104 64-65 0.4726915400481353 1104 66-67 0.7149929598001208 1104 68-69 0.6563866650046712 1104 70-71 0.7267364593372214 1104 72-73 0.8223008546350847 1104 74-75 0.8000533461209507 1104 76-77 0.7254443213637991 1104 78-79 0.8470051140374899 1104 80-81 1.049978249980331 1104 82-83 1.196595544176187 1104 84-85 1.2817489293065165 1104 86-87 1.0456959107952777 1104 88-89 1.2481314691405814 1104 90-91 1.4947360797764198 1104 92-93 1.412746641970731 1104 94-95 1.2643969850545673 1104 96-97 1.374288752984647 1104 98-99 1.2581950223358191 1104 100-101 1.0557570473801086 1105 1 0.12006641664799744 1105 2 -0.016328277951323855 1105 3 0.0286781788162358 1105 4 0.1497471167986717 1105 5 0.18496320838852398 1105 6 0.13099897168764585 1105 7 0.2558582963027689 1105 8 0.22127256397914152 1105 9 0.21545723436966568 1105 10-11 0.2919395989190505 1105 12-13 0.2942320781112002 1105 14-15 0.28825446953337064 1105 16-17 0.410086184103271 1105 18-19 0.3183592030022808 1105 20-21 0.3020097917440765 1105 22-23 0.3025928064787564 1105 24-25 0.2464249318233982 1105 26-27 0.29248517992625267 1105 28-29 0.3653621750507554 1105 30-31 0.4196305208547031 1105 32-33 0.4857880727827393 1105 34-35 0.6037598343726742 1105 36-37 0.5423548640209503 1105 38-39 0.4871489228944412 1105 40-41 0.16500324373013342 1105 42-43 0.4045898487678272 1105 44-45 0.5393685579212573 1105 46-47 0.511287234222273 1105 48-49 0.5928096848706161 1105 50-51 0.7232545012657567 1105 52-53 0.9169964029263937 1105 54-55 0.9156800181408471 1105 56-57 1.079710656490576 1105 58-59 0.8606804196099276 1105 60-61 0.8944713276328997 1105 62-63 0.8138156081320354 1105 64-65 0.7784044917175983 1105 66-67 0.5730676790154448 1105 68-69 0.4401561674694783 1105 70-71 0.5001166748172245 1105 72-73 0.6418265064112738 1105 74-75 0.7267275088490059 1105 76-77 0.6042345332863484 1105 78-79 0.7400923478637829 1105 80-81 0.9386246391656741 1105 82-83 1.1365001917342497 1105 84-85 1.1431660025392247 1105 86-87 0.9604620082694879 1105 88-89 1.0648411428713302 1105 90-91 1.2182394720618284 1105 92-93 1.1731691530242436 1105 94-95 1.194867013514191 1105 96-97 1.3713704346434383 1105 98-99 1.4990667888652673 1105 100-101 1.255262370854215 1106 1 0.46354481493305144 1106 2 0.30909331277038277 1106 3 0.3357947896006017 1106 4 0.3134041552200415 1106 5 0.24097005167990204 1106 6 0.4168791178977287 1106 7 0.3984837566446586 1106 8 0.4647124859271443 1106 9 0.5649071601652977 1106 10-11 0.5045840225984861 1106 12-13 0.5754110343031371 1106 14-15 0.6490171942077012 1106 16-17 0.7476645109322675 1106 18-19 0.633856990612891 1106 20-21 0.48008082181058853 1106 22-23 0.6863375846901647 1106 24-25 0.6533166621592414 1106 26-27 0.6913718396305342 1106 28-29 0.848942810459981 1106 30-31 0.8963671091029255 1106 32-33 0.8383549941261137 1106 34-35 0.6895913354994789 1106 36-37 0.7898420843804317 1106 38-39 0.9198995687472404 1106 40-41 0.87277079168814 1106 42-43 0.8537203522581791 1106 44-45 0.9886479510944639 1106 46-47 0.6033770489861894 1106 48-49 0.5452576044001063 1106 50-51 0.6157828775643353 1106 52-53 0.7250124667971178 1106 54-55 0.8803707645817838 1106 56-57 0.7527939165356514 1106 58-59 0.6538359602024642 1106 60-61 0.6448657100876858 1106 62-63 0.7132984449373971 1106 64-65 0.76632573890058 1106 66-67 0.91916775871643 1106 68-69 1.0637837989761039 1106 70-71 0.9197301250453371 1106 72-73 0.9369053141944867 1106 74-75 0.9035121238108026 1106 76-77 0.9018712616983748 1106 78-79 1.0101826298403687 1106 80-81 1.1481802374727117 1106 82-83 1.3809811047227605 1106 84-85 1.2000802690153414 1106 86-87 1.0135910963802992 1106 88-89 1.3677355940338671 1106 90-91 1.6693876471841271 1106 92-93 1.6945439262886843 1106 94-95 1.649720322479176 1106 96-97 1.7420590923689367 1106 98-99 1.878013155599728 1106 100-101 1.3133687167015182 1107 1 0.12135558287210912 1107 2 0.06123146713630234 1107 3 0.06431147674507187 1107 4 0.06145051563031956 1107 5 0.064866288579708 1107 6 0.12812722447575453 1107 7 0.16941910352528566 1107 8 0.0879892394969346 1107 9 0.22016647494321262 1107 10-11 0.1442975383773657 1107 12-13 0.24411325177025134 1107 14-15 0.25247048121685367 1107 16-17 0.19895368277560266 1107 18-19 0.32139358961937603 1107 20-21 0.176602861313917 1107 22-23 0.18530988029714024 1107 24-25 0.20412209061256448 1107 26-27 0.2826046701015059 1107 28-29 0.10501034286806998 1107 30-31 0.22754674496516003 1107 32-33 0.31834250719591495 1107 34-35 0.3406198981007691 1107 36-37 0.34059437543890425 1107 38-39 0.39432231641541904 1107 40-41 0.3748890642293361 1107 42-43 0.20649837240032554 1107 44-45 0.29106266307260853 1107 46-47 0.32681564633060844 1107 48-49 0.3929610390925973 1107 50-51 0.3626364715259811 1107 52-53 0.38138766686691383 1107 54-55 0.4206753713006961 1107 56-57 0.45102836758988474 1107 58-59 0.487906256041164 1107 60-61 0.5393598034693241 1107 62-63 0.4590645459971405 1107 64-65 0.4877320275646504 1107 66-67 0.32380387126185894 1107 68-69 0.5041936077243925 1107 70-71 0.5879300046101079 1107 72-73 0.5677990236709611 1107 74-75 0.44450100858347596 1107 76-77 0.43835827200116384 1107 78-79 0.5990819920485961 1107 80-81 0.7928439700206908 1107 82-83 0.8066561927963853 1107 84-85 0.7561525930862238 1107 86-87 0.7071441644033172 1107 88-89 0.7149188114852478 1107 90-91 1.1134738055721805 1107 92-93 1.1473240919514858 1107 94-95 1.0313028871201375 1107 96-97 1.0064879333157677 1107 98-99 0.8679907134536897 1107 100-101 0.5618031302806621 1108 1 -0.051419335133029875 1108 2 -0.12862486587783195 1108 3 0.004522760743494558 1108 4 0.015640442632943063 1108 5 0.010691554845216444 1108 6 0.027449102921245583 1108 7 -0.01826431337102008 1108 8 0.01641598355169549 1108 9 0.08040192878286234 1108 10-11 0.046592767273004654 1108 12-13 0.04883884601520805 1108 14-15 0.05691541816479173 1108 16-17 0.050201482191013724 1108 18-19 -0.03018832230706181 1108 20-21 -0.030000425728736957 1108 22-23 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-0.9788584724732488 2316 74-75 -1.110412799873874 2316 76-77 -1.549224764805981 2316 78-79 -1.2232398932895592 2316 80-81 -1.5870481437089445 2316 82-83 -1.5083980534965669 2316 84-85 -1.455292749463105 2316 86-87 -1.5088881446357085 2316 88-89 -1.762468271720195 2316 90-91 -1.9688092755717825 2316 92-93 -2.006450222248805 2316 94-95 -1.7627158101351483 2316 96-97 -1.494024024363405 2316 98-99 -1.3219243644768035 2316 100-101 -1.1152857214714018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 72.0 5 152.0 6 277.0 7 694.0 8 1534.0 9 2710.0 10 4615.0 11 6899.0 12 10208.0 13 13508.0 14 17660.0 15 23458.0 16 29970.0 17 37592.0 18 33430.0 19 24742.0 20 23804.0 21 25296.0 22 27486.0 23 31099.0 24 35906.0 25 42455.0 26 50931.0 27 61572.0 28 73405.0 29 87302.0 30 103638.0 31 123735.0 32 147524.0 33 179934.0 34 221160.0 35 276208.0 36 333042.0 37 316625.0 38 117307.0 39 3722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.15435112792737 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20.60398015665335 28.09660678129623 31.333200251757138 19.966212810293285 20-21 21.636246460604692 27.005684119750704 31.61901315262292 19.73905626702168 22-23 21.294444593949233 27.117011239502254 31.407928100603243 20.180616065945266 24-25 21.03293086969541 27.362118263216 31.491817340325824 20.113133526762773 26-27 20.914323470521015 27.506542071090173 31.400957160731107 20.178177297657708 28-29 20.626853189997213 27.486959888754374 31.59775805683263 20.28842886441578 30-31 21.016543358905423 27.178409776780647 31.62371566271352 20.181331201600404 32-33 20.877657037404926 27.339216137941918 31.387819867208126 20.39530695744503 34-35 21.13620388408009 27.320125318819915 31.35629506155484 20.187375735545157 36-37 21.225167464845136 27.215482479905624 31.1986293529111 20.360720702338142 38-39 20.946618052117717 27.578145946070787 31.21080150743929 20.264434494372207 40-41 21.181800033659904 27.300311012693157 31.192126956481918 20.325761997165017 42-43 21.004827382466985 27.479107015580297 30.86928029886914 20.646785303083583 44-45 21.228169215478477 27.55648846974693 30.591846894964213 20.623495419810382 46-47 21.37938735176675 27.866013549024693 29.96403782866165 20.790561270546906 48-49 21.40190151652992 28.087333518898085 29.64690080760832 20.86386415696368 50-51 21.393851224585784 28.11655143854739 29.474466592146282 21.015130744720548 52-53 21.545749472120704 28.39185292561812 29.17044727452816 20.891950327733014 54-55 21.42365964557125 28.296801947269483 29.202133641811667 21.0774047653476 56-57 21.5610763176577 28.503981917445632 28.940059866830943 20.994881898065724 58-59 21.69520553791269 28.20394794580577 29.15126053626689 20.949585980014657 60-61 21.732386946839163 28.244005606341858 29.25700707379923 20.76660037301975 62-63 21.63440509523031 28.16551063762275 29.37629859971137 20.82378566743557 64-65 21.472594328340282 28.1913102189847 29.296405643342986 21.039689809332028 66-67 21.624167244212707 28.407272280340546 28.873071279055452 21.09548919639129 68-69 21.53099910390846 28.313701123146917 28.970225741966605 21.18507403097802 70-71 21.507344671799288 28.295984730665914 29.038906758094164 21.157763839440637 72-73 21.645836517283907 28.23150816734784 28.914722875301667 21.20793244006659 74-75 21.45264211502552 28.589632207370315 28.659379818620064 21.298345858984106 76-77 21.485968293955494 28.468805064741176 28.775631275146274 21.269595366157056 78-79 21.444534370675928 28.43617390885229 28.725606951855546 21.393684768616236 80-81 21.382089361594446 28.68067190098944 28.620461256116965 21.31677748129915 82-83 21.563205039067473 28.543375479532003 28.61987165351832 21.273547827882204 84-85 21.435906148974553 28.54942297569535 28.477102890734812 21.53756798459528 86-87 21.319441780656163 28.57105560122209 28.596139282108666 21.51336333601308 88-89 21.5375241296264 28.383228674551486 28.52087699694499 21.558370198877125 90-91 21.430627041788394 28.4579638859886 28.555023831198838 21.556385241024163 92-93 21.56273415216007 28.413538094240653 28.484932223775512 21.53879552982377 94-95 21.379862135889574 28.39750041972962 28.436119561488983 21.786517882891822 96-97 21.399403735639396 28.400900923516897 28.329987839741655 21.869707501102052 98-99 21.23694590382545 28.35257306277213 28.2538238460179 22.15665718738452 100-101 21.280067092905842 28.560073133837978 28.18414158916681 21.975718184089374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1652.0 1 1143.5 2 699.0 3 776.5 4 707.5 5 692.0 6 1161.0 7 2597.5 8 4230.5 9 4976.5 10 4435.0 11 3559.0 12 3468.5 13 3697.5 14 4000.0 15 4320.0 16 4687.5 17 5057.0 18 5464.0 19 5876.0 20 6283.5 21 7151.5 22 8518.0 23 10231.5 24 12125.5 25 14735.0 26 18918.5 27 23405.5 28 26712.5 29 30986.5 30 37124.5 31 42452.5 32 48308.5 33 55641.5 34 64103.0 35 71101.0 36 78248.5 37 85753.0 38 87831.0 39 87408.0 40 87417.5 41 88228.5 42 88068.0 43 88951.0 44 91520.5 45 91822.0 46 90312.0 47 90024.0 48 92851.0 49 91024.5 50 84080.0 51 78088.5 52 72826.5 53 68687.5 54 63623.0 55 56878.0 56 50586.0 57 44785.5 58 39392.5 59 34566.5 60 29494.0 61 23496.0 62 17684.0 63 13071.5 64 9633.0 65 7132.5 66 5047.5 67 3668.5 68 2529.0 69 1563.0 70 1057.0 71 701.0 72 463.5 73 325.0 74 242.5 75 183.5 76 125.0 77 64.5 78 28.0 79 14.5 80 9.0 81 8.5 82 5.0 83 1.5 84 1.0 85 1.5 86 1.0 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012531625283517981 2 0.002530424336094977 3 0.0016066186260920486 4 2.0082732826150608E-4 5 0.0015262876947874462 6 3.6148919087071094E-4 7 0.0 8 0.0 9 0.0 10-11 1.606618626092049E-4 12-13 6.828129160891207E-4 14-15 2.008273282615061E-5 16-17 0.0 18-19 0.0 20-21 0.0018275286871797056 22-23 0.0 24-25 0.003072658122401043 26-27 0.0018476114200058564 28-29 0.0030124099239225914 30-31 0.013395182795042457 32-33 6.22564717610669E-4 34-35 0.002048438748267362 36-37 0.00297224445827029 38-39 0.001948025084136609 40-41 0.0036952228400117127 42-43 0.0019881905497889105 44-45 4.619028550014641E-4 46-47 6.024819847845184E-5 48-49 1.606618626092049E-4 50-51 8.033093130460244E-5 52-53 8.836402443506267E-4 54-55 0.0024500934047903744 56-57 3.4140645804456033E-4 58-59 0.0010643848397859823 60-61 8.23392045872175E-4 62-63 0.0 64-65 2.6107552673995796E-4 66-67 2.008273282615061E-5 68-69 0.0 70-71 2.0082732826150608E-4 72-73 2.008273282615061E-5 74-75 4.0165465652301216E-4 76-77 7.229783817414219E-4 78-79 2.4099279391380735E-4 80-81 0.004337870290448532 82-83 0.0013656258321782413 84-85 0.0029923271910964407 86-87 0.001385708565004392 88-89 9.238057100029282E-4 90-91 1.0041366413075304E-4 92-93 2.008273282615061E-5 94-95 1.2049639695690367E-4 96-97 0.0013455430993520908 98-99 0.0024500934047903744 100-101 7.631438473937231E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2489701.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.29995787703577 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22711894738877 38.820751942821566 2 14.408703879382944 15.071492119088198 3 4.949572387115499 7.765872820664398 4 2.2166460284873373 4.637219756727455 5 1.1543582293748802 3.0186443385657915 6 0.6823117486407773 2.1410925427751564 7 0.4491296340213838 1.6442622658452803 8 0.29899441788088676 1.2509916368511367 9 0.22237111606586524 1.0467000002982765 >10 1.2107410385255455 11.750955731695093 >50 0.11403744871179439 4.143553506394323 >100 0.06301537931929341 5.964356816608004 >500 0.002153663137975246 0.721517182036952 >1k 6.15332325134513E-4 0.5517981258911981 >5k 7.691654064181412E-5 0.35955177146719614 >10k+ 1.5383308128362824E-4 1.1112394422700633 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15089 0.6060567112275731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12535 0.5034741119515957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8938 0.35899893200026833 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3044 0.12226367744560493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.016546565230122E-5 0.0 0.0 0.0 0.0 7 4.016546565230122E-5 0.0 0.0 0.0 0.0 8 4.016546565230122E-5 0.0 0.0 0.0 0.0 9 4.016546565230122E-5 0.0 0.0 4.016546565230122E-5 0.0 10-11 4.016546565230122E-5 0.0 0.0 4.016546565230122E-5 0.0 12-13 4.016546565230122E-5 2.008273282615061E-5 0.0 4.016546565230122E-5 0.0 14-15 4.016546565230122E-5 8.033093130460244E-5 0.0 4.016546565230122E-5 4.016546565230122E-5 16-17 4.016546565230122E-5 1.2049639695690366E-4 0.0 1.2049639695690366E-4 8.033093130460244E-5 18-19 4.016546565230122E-5 1.2049639695690366E-4 0.0 1.2049639695690366E-4 8.033093130460244E-5 20-21 4.016546565230122E-5 1.2049639695690366E-4 0.0 2.4099279391380735E-4 8.033093130460244E-5 22-23 4.016546565230122E-5 1.2049639695690366E-4 0.0 4.016546565230122E-4 8.033093130460244E-5 24-25 4.016546565230122E-5 1.4057912978305428E-4 0.0 6.426474504368196E-4 8.033093130460244E-5 26-27 4.016546565230122E-5 1.606618626092049E-4 0.0 8.434747786983256E-4 8.033093130460244E-5 28-29 4.016546565230122E-5 1.606618626092049E-4 0.0 0.0014861222291351452 8.033093130460244E-5 30-31 4.016546565230122E-5 1.606618626092049E-4 0.0 0.0025103416032688265 8.033093130460244E-5 32-33 4.016546565230122E-5 1.606618626092049E-4 0.0 0.005723578855452924 8.033093130460244E-5 34-35 4.016546565230122E-5 1.807445954353555E-4 0.0 0.01018194554285836 8.033093130460244E-5 36-37 4.016546565230122E-5 2.209100610876567E-4 0.0 0.017311315696141825 1.2049639695690366E-4 38-39 4.016546565230122E-5 2.8115825956610856E-4 0.0 0.028658059742916922 1.2049639695690366E-4 40-41 4.016546565230122E-5 3.61489190870711E-4 0.0 0.042173738934916286 1.2049639695690366E-4 42-43 4.016546565230122E-5 4.016546565230122E-4 0.0 0.05657305837126627 1.2049639695690366E-4 44-45 4.016546565230122E-5 4.016546565230122E-4 0.0 0.07294048562457901 1.2049639695690366E-4 46-47 4.016546565230122E-5 4.016546565230122E-4 0.0 0.09436876155008173 1.2049639695690366E-4 48-49 4.016546565230122E-5 4.418201221753134E-4 0.0 0.11790572442233023 1.606618626092049E-4 50-51 4.016546565230122E-5 4.8198558782761464E-4 0.0 0.14461575908111055 1.606618626092049E-4 52-53 4.016546565230122E-5 4.8198558782761464E-4 0.0 0.17554316763338246 1.606618626092049E-4 54-55 6.024819847845184E-5 4.8198558782761464E-4 0.0 0.21024612995697073 1.606618626092049E-4 56-57 8.033093130460244E-5 4.8198558782761464E-4 0.0 0.25103416032688264 1.606618626092049E-4 58-59 8.033093130460244E-5 4.8198558782761464E-4 0.0 0.2985298234607288 1.606618626092049E-4 60-61 8.033093130460244E-5 5.221510534799159E-4 0.0 0.35243187836611706 1.606618626092049E-4 62-63 8.033093130460244E-5 5.221510534799159E-4 0.0 0.411093540951303 1.606618626092049E-4 64-65 8.033093130460244E-5 5.221510534799159E-4 0.0 0.47567960972020334 1.606618626092049E-4 66-67 8.033093130460244E-5 6.024819847845183E-4 0.0 0.5493029082608715 1.807445954353555E-4 68-69 8.033093130460244E-5 6.426474504368196E-4 0.0 0.6351766738254915 2.008273282615061E-4 70-71 8.033093130460244E-5 6.426474504368196E-4 0.0 0.7338029747347171 2.008273282615061E-4 72-73 8.033093130460244E-5 6.426474504368196E-4 0.0 0.8354416855678654 2.4099279391380732E-4 74-75 8.033093130460244E-5 6.426474504368196E-4 0.0 0.9451335722643 2.4099279391380732E-4 76-77 8.033093130460244E-5 6.426474504368196E-4 0.0 1.066995595053382 2.4099279391380732E-4 78-79 8.033093130460244E-5 6.426474504368196E-4 0.0 1.2057873616149086 2.4099279391380732E-4 80-81 8.033093130460244E-5 6.627301832629702E-4 0.0 1.3601432461167025 2.4099279391380732E-4 82-83 8.033093130460244E-5 6.828129160891208E-4 0.0 1.5229740438711317 2.4099279391380732E-4 84-85 8.033093130460244E-5 6.828129160891208E-4 0.0 1.6929944599773226 2.4099279391380732E-4 86-87 8.033093130460244E-5 6.828129160891208E-4 0.0 1.877876098374865 2.4099279391380732E-4 88-89 8.033093130460244E-5 6.828129160891208E-4 0.0 2.0771570562087573 2.4099279391380732E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9520 0.0 42.313652 1 GTATCAA 15220 0.0 35.986225 1 TATCAAC 19875 0.0 27.530535 2 ATCAACG 19915 0.0 27.450285 3 TCAACGC 20120 0.0 27.194202 4 CAACGCA 20495 0.0 26.673452 5 AACGCAG 21035 0.0 25.989748 6 ACGCAGA 23885 0.0 22.709633 7 CGCAGAG 23920 0.0 22.458433 8 GAGTACT 13825 0.0 22.434547 12-13 GTACATG 12385 0.0 22.169374 1 AGTACTT 14415 0.0 21.895235 12-13 TACACCG 155 3.911676E-5 21.44955 5 GCAGAGT 24825 0.0 21.161373 9 TACATGG 12725 0.0 20.82791 2 GTACTTT 14860 0.0 20.792698 14-15 AGAGTAC 21275 0.0 20.729572 10-11 ACATGGG 12670 0.0 20.467646 3 TACTTTT 16805 0.0 18.48509 14-15 CAGAGTA 24375 0.0 18.473183 10-11 >>END_MODULE