FastQCFastQC Report
Thu 26 May 2016
SRR936806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454468
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160250.6528909727077313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110140.4487326785274854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79710.32475469225917797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.10230322823520208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74400.044.7127721
GTATCAA132650.041.3074651
ATCAACG167350.032.5151863
TATCAAC168450.032.3599432
TCAACGC169050.032.2169764
CAACGCA170400.031.909865
AACGCAG174700.031.2300856
ACGCAGA196950.027.5111337
CGCAGAG198350.027.1999158
GCAGAGT207550.025.3529649
GTACATG130150.024.3551
TACATGG128050.024.2353292
TATCACG1000.001649270423.7621292
GAGTACT111200.023.18611512-13
ACATGGG127400.023.1275023
AGTACTT115950.022.62566612-13
AGAGTAC184500.021.8713410-11
GTACTTT119650.021.2483614-15
CAGAGTA203500.020.43655410-11
CATGGGA84400.019.3700774
Produced by FastQC (version 0.11.5)