FastQCFastQC Report
Thu 26 May 2016
SRR936806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454468
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144460.5885593130568416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118350.4821818821838378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85390.34789616324189193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29240.11912968512932334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87700.042.369961
GTATCAA142650.036.5081331
TATCAAC186450.027.8468362
ATCAACG186950.027.771233
TCAACGC189000.027.4912194
CAACGCA192450.026.9490365
AACGCAG198150.026.2206846
ACGCAGA225200.022.8813767
CGCAGAG225650.022.7726048
GAGTACT131500.021.85061512-13
AGTACTT137200.021.68707812-13
GCAGAGT236800.021.3794389
GTACATG125700.021.2825661
AGAGTAC204550.020.74511510-11
GTACTTT140150.020.4350614-15
TACATGG127350.020.3662852
TAGGCCG1456.1540224E-419.6526785
ACATGGG126450.019.6464143
TACTTTT159650.018.400214-15
CAGAGTA232750.018.39487310-11
Produced by FastQC (version 0.11.5)