##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936806_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2454468 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48780183730242 31.0 31.0 34.0 30.0 34.0 2 31.582755611399293 31.0 31.0 34.0 30.0 34.0 3 31.69695591875714 31.0 31.0 34.0 30.0 34.0 4 35.52462162880103 37.0 35.0 37.0 33.0 37.0 5 35.39572282058678 37.0 35.0 37.0 33.0 37.0 6 35.450969823195905 37.0 35.0 37.0 33.0 37.0 7 35.38994600866664 37.0 35.0 37.0 33.0 37.0 8 35.36484525363541 37.0 35.0 37.0 33.0 37.0 9 36.915359255040194 39.0 37.0 39.0 33.0 39.0 10-11 36.763608244230525 39.0 36.0 39.0 32.0 39.0 12-13 36.65310751657793 38.0 35.0 39.0 32.0 39.0 14-15 37.611769026933736 39.0 36.5 41.0 32.0 41.0 16-17 37.48327193509958 39.0 36.0 40.0 32.0 41.0 18-19 37.5392714429359 39.0 36.5 40.5 32.0 41.0 20-21 37.68049776978148 39.0 37.0 41.0 32.0 41.0 22-23 37.585264912803915 39.0 36.5 41.0 32.0 41.0 24-25 37.465267829933005 39.0 36.0 41.0 32.0 41.0 26-27 37.20860182328717 39.0 36.0 40.0 31.5 41.0 28-29 36.99747032758219 39.0 36.0 40.0 31.0 41.0 30-31 36.87134829217574 39.0 36.0 40.0 31.0 41.0 32-33 36.71942922050725 39.0 35.0 40.0 30.0 41.0 34-35 36.54355342990823 38.0 35.0 40.0 30.0 41.0 36-37 36.30502862534773 38.0 35.0 40.0 30.0 41.0 38-39 36.10307691931612 38.0 35.0 40.0 30.0 41.0 40-41 35.802252667380465 38.0 35.0 40.0 29.0 41.0 42-43 35.47490739337404 38.0 34.0 40.0 27.5 41.0 44-45 35.139201244424456 38.0 34.0 40.0 27.0 41.0 46-47 34.68398365755838 38.0 33.5 40.0 25.0 41.0 48-49 34.21628963995457 38.0 33.0 40.0 24.0 41.0 50-51 33.918302662735876 38.0 33.0 40.0 23.0 41.0 52-53 33.6335028609051 37.5 33.0 40.0 22.5 41.0 54-55 33.29369684184108 37.0 32.0 40.0 21.0 41.0 56-57 32.987766799159736 37.0 31.5 39.0 19.5 40.0 58-59 32.54372821320139 36.0 31.0 39.0 18.0 40.0 60-61 32.12214724331301 36.0 31.0 39.0 16.0 40.0 62-63 31.713417938225312 35.5 30.0 39.0 13.0 40.0 64-65 31.22720279914018 35.0 29.5 38.0 10.0 40.0 66-67 30.686206949937826 34.5 29.0 38.0 8.0 40.0 68-69 30.151505540100747 34.0 28.0 37.5 7.5 39.5 70-71 29.65904179643002 34.0 28.0 37.0 6.5 39.0 72-73 29.137284332083368 34.0 26.5 36.0 2.0 39.0 74-75 28.546437761665665 33.0 26.0 36.0 2.0 38.5 76-77 27.36969416590479 31.5 25.5 34.5 2.0 36.5 78-79 28.06193602849986 32.5 26.0 35.0 2.0 37.0 80-81 27.730283914885018 33.0 26.0 35.0 2.0 37.0 82-83 27.352439714023568 32.0 26.0 35.0 2.0 36.0 84-85 26.8360108993069 32.0 25.0 35.0 2.0 36.0 86-87 26.273033097192545 32.0 24.5 34.0 2.0 35.5 88-89 25.667519193568626 31.0 22.0 34.0 2.0 35.0 90-91 24.932235824626765 31.0 19.5 34.0 2.0 35.0 92-93 24.190077035023478 31.0 16.5 34.0 2.0 35.0 94-95 23.317928773159807 30.0 4.5 34.0 2.0 35.0 96-97 22.235576304111525 29.5 2.0 34.0 2.0 35.0 98-99 20.93413032885334 29.0 2.0 34.0 2.0 35.0 100-101 17.59125887972465 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39162206548180833 1101 2 0.3462643615559706 1101 3 0.34629533187394657 1101 4 0.22288705888041704 1101 5 0.3935697079547822 1101 6 0.3321999292146458 1101 7 0.3476767048580527 1101 8 0.3428901480228461 1101 9 0.466741249506498 1101 10-11 0.44099030911549875 1101 12-13 0.33005976533135595 1101 14-15 0.44264509554268017 1101 16-17 0.4155783016142749 1101 18-19 0.4484420836270999 1101 20-21 0.4497877643536512 1101 22-23 0.49976115488955486 1101 24-25 0.5249661723663266 1101 26-27 0.48421314720519604 1101 28-29 0.548252300733644 1101 30-31 0.42261208482681667 1101 32-33 0.51079910747805 1101 34-35 0.6293962421344474 1101 36-37 0.5819829422035667 1101 38-39 0.5409012336514181 1101 40-41 0.6237631915159128 1101 42-43 0.7161300947204055 1101 44-45 0.6476668798493037 1101 46-47 0.8184557603738156 1101 48-49 0.7868766293557528 1101 50-51 0.8231286251230188 1101 52-53 0.8802252145943541 1101 54-55 0.9550339045778173 1101 56-57 0.955842161307352 1101 58-59 0.9081179819822367 1101 60-61 0.9477035670282348 1101 62-63 0.7405717968052485 1101 64-65 1.031135962242022 1101 66-67 0.9101929908601321 1101 68-69 0.9162650264017707 1101 70-71 1.0412144311967673 1101 72-73 1.1025494774178881 1101 74-75 1.013604195281932 1101 76-77 1.0304388807506903 1101 78-79 1.3589496711678137 1101 80-81 1.6836946700059876 1101 82-83 1.711809357148578 1101 84-85 1.45701403178656 1101 86-87 1.3240330488378902 1101 88-89 1.329960430319307 1101 90-91 1.1192948802858744 1101 92-93 1.41265321164364 1101 94-95 1.6337295526709177 1101 96-97 1.812896776788044 1101 98-99 2.148394932569108 1101 100-101 2.0505164305809416 1102 1 0.33876864803088935 1102 2 0.29590592177002506 1102 3 0.28314134029266924 1102 4 0.286350572530381 1102 5 0.30701355006718956 1102 6 0.31386396720682797 1102 7 0.28031648592951086 1102 8 0.29984534217918224 1102 9 0.38229882444112917 1102 10-11 0.37103118413333647 1102 12-13 0.3486337007181888 1102 14-15 0.418432914508827 1102 16-17 0.4466521934800056 1102 18-19 0.4548311553046176 1102 20-21 0.3237266328746671 1102 22-23 0.4679508859987891 1102 24-25 0.44390585908403324 1102 26-27 0.5449914089376122 1102 28-29 0.48959229801792503 1102 30-31 0.6680646520495017 1102 32-33 0.708409276792537 1102 34-35 0.652787267038967 1102 36-37 0.6522353994049155 1102 38-39 0.7827279709267216 1102 40-41 0.7765100693121454 1102 42-43 0.8208152282605496 1102 44-45 0.92514105442811 1102 46-47 0.8542800674308495 1102 48-49 1.033348634842902 1102 50-51 0.9817640819378965 1102 52-53 0.8249676915368767 1102 54-55 0.7618590576850508 1102 56-57 0.9223845317102217 1102 58-59 0.8675094873597047 1102 60-61 0.947040131368091 1102 62-63 0.8379647087846962 1102 64-65 0.7616653180949164 1102 66-67 0.6669403282391855 1102 68-69 0.799732578809877 1102 70-71 0.9362497181052696 1102 72-73 0.9664494206663719 1102 74-75 1.0513013023473192 1102 76-77 0.9620848139649496 1102 78-79 1.1000431649373361 1102 80-81 1.1493244899678992 1102 82-83 1.1254645377263337 1102 84-85 1.0199559885648846 1102 86-87 1.025752132318324 1102 88-89 1.0624017551712477 1102 90-91 1.1258296606087654 1102 92-93 1.3616661530781649 1102 94-95 1.6070295314326195 1102 96-97 1.7929616754535118 1102 98-99 1.9316208558257415 1102 100-101 1.821072665065671 1103 1 0.37141233504360827 1103 2 0.2961361514943661 1103 3 0.3253503904867614 1103 4 0.29248174250649583 1103 5 0.31161974894235556 1103 6 0.2902906895778514 1103 7 0.3118956612899524 1103 8 0.27298948604147455 1103 9 0.30756802074687073 1103 10-11 0.2445957579556577 1103 12-13 0.33135323518887105 1103 14-15 0.5710800349125549 1103 16-17 0.4628970263468659 1103 18-19 0.5348407976784415 1103 20-21 0.5682273913492466 1103 22-23 0.5941107584820031 1103 24-25 0.5178188258306804 1103 26-27 0.4874945435112963 1103 28-29 0.5209356282949003 1103 30-31 0.4841239374068138 1103 32-33 0.5985824785339418 1103 34-35 0.6103848880519607 1103 36-37 0.6198788979020122 1103 38-39 0.6265503940183592 1103 40-41 0.674898541461161 1103 42-43 0.5765098040642869 1103 44-45 0.8402837527365179 1103 46-47 0.7335325989550725 1103 48-49 0.8126305266734377 1103 50-51 0.8216457262113153 1103 52-53 0.8440711432981303 1103 54-55 0.6942525403388089 1103 56-57 0.5609206557166075 1103 58-59 0.7287667983567445 1103 60-61 0.8502020598749453 1103 62-63 0.8246924063831642 1103 64-65 0.7027269097604396 1103 66-67 0.7349098384434996 1103 68-69 0.7896826903549758 1103 70-71 0.8733925912920135 1103 72-73 1.0007729022532104 1103 74-75 0.7498363353696398 1103 76-77 0.8999095521889409 1103 78-79 1.1292975816178377 1103 80-81 1.2399832598352383 1103 82-83 1.2879008334824817 1103 84-85 1.160925167463006 1103 86-87 1.0415373079306605 1103 88-89 1.1581211669062306 1103 90-91 0.9805025619284535 1103 92-93 1.1009105343097083 1103 94-95 1.3788297594162024 1103 96-97 1.9294790778525552 1103 98-99 1.8437573315140448 1103 100-101 1.2988735460352991 1104 1 0.25755983675500715 1104 2 0.2553956416426324 1104 3 0.24215655478250042 1104 4 0.2285159105114687 1104 5 0.08067342489083984 1104 6 0.2044115097554311 1104 7 0.16073584151876474 1104 8 0.2880264213299242 1104 9 0.25822694780406863 1104 10-11 0.3580415111472206 1104 12-13 0.287866694142096 1104 14-15 0.3145966125203401 1104 16-17 0.38276653024063734 1104 18-19 0.3440371449303612 1104 20-21 0.3653635988886563 1104 22-23 0.3165318469814764 1104 24-25 0.390346152135173 1104 26-27 0.5061937195602582 1104 28-29 0.3840173180592572 1104 30-31 0.4760133575240886 1104 32-33 0.42942073472266884 1104 34-35 0.39849997341884347 1104 36-37 0.5339619362418375 1104 38-39 0.7061743183281166 1104 40-41 0.6538319200382432 1104 42-43 0.6062878546584116 1104 44-45 0.6240430644836721 1104 46-47 0.7622027561912788 1104 48-49 0.8700944585962347 1104 50-51 0.8282427302532653 1104 52-53 0.8289421147801619 1104 54-55 0.7876178961410005 1104 56-57 0.7634134187236512 1104 58-59 0.7811664291337337 1104 60-61 0.9253750475611398 1104 62-63 0.8984361189540522 1104 64-65 0.9203625152698827 1104 66-67 0.8919411919376898 1104 68-69 0.9599453024637263 1104 70-71 1.055983889331916 1104 72-73 1.0793374592528977 1104 74-75 1.0086827165425767 1104 76-77 1.0693630426285985 1104 78-79 1.2700280202484429 1104 80-81 1.3099123055439428 1104 82-83 1.3742188729640326 1104 84-85 1.111626303917074 1104 86-87 1.1698928369129895 1104 88-89 0.9967989182918195 1104 90-91 1.1890747768343815 1104 92-93 1.2745831343778597 1104 94-95 1.3793127765982014 1104 96-97 1.5700160616670544 1104 98-99 1.738081708426602 1104 100-101 1.4217881952108655 1105 1 0.28949756345948785 1105 2 0.24296721713484004 1105 3 0.27431832247699717 1105 4 0.24688973503527478 1105 5 0.2850698752144609 1105 6 0.21874434426193545 1105 7 0.16033370087423293 1105 8 0.23401443716921477 1105 9 0.1768498162431129 1105 10-11 0.29458852095748256 1105 12-13 0.365223527761799 1105 14-15 0.4823455486643482 1105 16-17 0.49698845275620585 1105 18-19 0.5162780474990143 1105 20-21 0.43649396512608263 1105 22-23 0.15940139452705893 1105 24-25 0.25866504412376656 1105 26-27 0.3135556646154072 1105 28-29 0.35078461226239455 1105 30-31 0.44356485677584345 1105 32-33 0.34252553146612286 1105 34-35 0.3513072599856173 1105 36-37 0.44793130457926367 1105 38-39 0.6624062666471957 1105 40-41 0.7138024215131722 1105 42-43 0.7253497522942212 1105 44-45 0.7584627318092814 1105 46-47 0.8067296404942468 1105 48-49 0.6815647660829427 1105 50-51 0.5868928802092341 1105 52-53 0.7169907165254372 1105 54-55 0.5860018819521429 1105 56-57 0.4288616546625832 1105 58-59 0.7651337652136974 1105 60-61 0.7849687192345485 1105 62-63 0.9007603440789183 1105 64-65 0.7802230285072653 1105 66-67 0.6269311230256882 1105 68-69 0.7292163994516727 1105 70-71 0.7564359742433275 1105 72-73 0.6924564202150769 1105 74-75 0.7317628182786144 1105 76-77 0.6766904393067819 1105 78-79 0.884744458748024 1105 80-81 1.0503436983793115 1105 82-83 1.1303739650006612 1105 84-85 0.49207530965049884 1105 86-87 0.6471066735071744 1105 88-89 0.49435054071069473 1105 90-91 0.6769058038634057 1105 92-93 0.8946429935978415 1105 94-95 0.9719943095361678 1105 96-97 0.9067185083818785 1105 98-99 0.9710899421485273 1105 100-101 1.0987659820208968 1106 1 0.1915137152495312 1106 2 0.17580925364283218 1106 3 0.2661559294501039 1106 4 0.23024305464983286 1106 5 0.1501742086756792 1106 6 0.2070416209065229 1106 7 0.15171053475557983 1106 8 0.2115381464369932 1106 9 0.25931716546612193 1106 10-11 0.27713539253901587 1106 12-13 0.18411661965323844 1106 14-15 0.4769035305467 1106 16-17 0.4048212782414069 1106 18-19 0.444569363137461 1106 20-21 0.23700489945038328 1106 22-23 0.3646042748344982 1106 24-25 0.24849861156399555 1106 26-27 0.27416286284739755 1106 28-29 0.40461750750404946 1106 30-31 0.34958027295236604 1106 32-33 0.321934186973877 1106 34-35 0.20286742651330059 1106 36-37 0.3530778089881892 1106 38-39 0.3743130593405226 1106 40-41 0.5709165969760193 1106 42-43 0.6281916302648156 1106 44-45 0.5110124887714562 1106 46-47 0.7117997932925917 1106 48-49 0.8142114845173509 1106 50-51 0.4091148769560391 1106 52-53 0.3750684822896062 1106 54-55 0.6343525539921728 1106 56-57 0.5758679929877317 1106 58-59 0.6898798675717828 1106 60-61 0.7153795530461338 1106 62-63 0.7616917195810871 1106 64-65 0.5536867852728484 1106 66-67 0.42033247947166075 1106 68-69 0.6201785629164114 1106 70-71 0.645012784026985 1106 72-73 0.7404864028076723 1106 74-75 0.7764911010332334 1106 76-77 0.5472518202309296 1106 78-79 0.6348788667535281 1106 80-81 1.0551733182383423 1106 82-83 0.9668158150686743 1106 84-85 0.8547092499706821 1106 86-87 0.7715988623696042 1106 88-89 0.8056189350122267 1106 90-91 0.8010760349792214 1106 92-93 1.0119973849252126 1106 94-95 0.954989715122391 1106 96-97 1.0902400044675886 1106 98-99 1.1504940951941904 1106 100-101 0.8454741432090387 1107 1 0.1855681804284579 1107 2 0.12380723598141685 1107 3 0.1629989018489688 1107 4 0.16614856456029514 1107 5 0.2018987784270294 1107 6 0.08620085770935049 1107 7 0.2366668237327616 1107 8 0.14175454672295018 1107 9 0.11240862685745157 1107 10-11 0.09193871791702435 1107 12-13 0.18706940250898185 1107 14-15 0.25703209611894096 1107 16-17 0.37503488328508894 1107 18-19 0.3097150797401156 1107 20-21 0.2635969450006357 1107 22-23 0.24346257538688576 1107 24-25 0.2756775411990233 1107 26-27 0.3735563056554341 1107 28-29 0.30957881597850445 1107 30-31 0.30513566124901814 1107 32-33 0.2780549824474008 1107 34-35 0.2671101786408414 1107 36-37 0.28792841989915985 1107 38-39 0.2956874620586305 1107 40-41 0.4128262457656362 1107 42-43 0.18497891064073002 1107 44-45 0.36051918820414386 1107 46-47 0.4481239666489998 1107 48-49 0.4398459454683774 1107 50-51 0.4459654876074737 1107 52-53 0.388779314346543 1107 54-55 0.3426713585109127 1107 56-57 0.44034364304052787 1107 58-59 0.4594031879250977 1107 60-61 0.5070504967103275 1107 62-63 0.4837013716019669 1107 64-65 0.40725703247530376 1107 66-67 0.32553269421478603 1107 68-69 0.5561250800951463 1107 70-71 0.44411460805882896 1107 72-73 0.5400319747948288 1107 74-75 0.608049593943452 1107 76-77 0.6054050579039938 1107 78-79 0.7265644354782701 1107 80-81 0.850785631371398 1107 82-83 0.8237351622070079 1107 84-85 0.7196050298685819 1107 86-87 0.6581778193341634 1107 88-89 0.4163630858639529 1107 90-91 0.3092830687379369 1107 92-93 0.6242945562931546 1107 94-95 0.6368596751052671 1107 96-97 0.5319112530908896 1107 98-99 0.7679441023380171 1107 100-101 0.890039375975249 1108 1 0.18845865643430315 1108 2 0.16482413883556646 1108 3 0.137972156570072 1108 4 0.12050617058797997 1108 5 0.10988164469685557 1108 6 0.16712432399476285 1108 7 0.07005038960152632 1108 8 0.19395792675340573 1108 9 -0.005106460803162349 1108 10-11 0.1542822028157218 1108 12-13 0.1335980280968272 1108 14-15 0.1741290266295792 1108 16-17 0.19337746359286 1108 18-19 0.08007111110953957 1108 20-21 0.2565407710662768 1108 22-23 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-1.3072720733358416 2316 60-61 -1.0812200353521177 2316 62-63 -1.146307970188296 2316 64-65 -0.7063278924222978 2316 66-67 -0.9148866336474555 2316 68-69 -1.187408177856046 2316 70-71 -0.8041238681913825 2316 72-73 -1.0067561130606961 2316 74-75 -0.9111637411860904 2316 76-77 -1.0867458690607243 2316 78-79 -1.1992354139046846 2316 80-81 -1.271236811009853 2316 82-83 -1.2316106202213 2316 84-85 -1.4202437420798404 2316 86-87 -1.3916121192117288 2316 88-89 -0.9904978296756646 2316 90-91 -1.034630817476259 2316 92-93 -0.8851283028983126 2316 94-95 -0.9700499922121573 2316 96-97 -1.173989276350838 2316 98-99 -1.2566874159347883 2316 100-101 -1.397272941642676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 52.0 5 112.0 6 251.0 7 630.0 8 1350.0 9 2419.0 10 3993.0 11 6337.0 12 9398.0 13 12806.0 14 16294.0 15 22045.0 16 28412.0 17 36115.0 18 32044.0 19 23918.0 20 22831.0 21 24161.0 22 26274.0 23 29406.0 24 34425.0 25 40588.0 26 48670.0 27 58821.0 28 70520.0 29 84186.0 30 100871.0 31 120420.0 32 145547.0 33 177106.0 34 221389.0 35 277482.0 36 338199.0 37 320080.0 38 113973.0 39 3318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.295510133557414 22.32235201876503 16.532568626763805 20.849569220913754 2 16.08725714651252 23.34424882930389 39.04736526655771 21.521128757625874 3 19.613248638099 27.000558206242946 31.28664501749997 22.09954813815808 4 13.657710614972618 19.56290306612393 37.77459627691063 29.004790041992823 5 12.747156395111245 39.10401337697256 34.55473447939937 13.594095748516827 6 29.264671611118985 39.13406082295634 17.62760810081859 13.973659465106083 7 26.218960687203907 34.620292462562155 21.7011588661983 17.459587984035643 8 25.502584066181427 36.146288498715606 20.17661690022062 18.174510534882344 9 26.209356247201534 16.818926471612777 21.05190251080988 35.91981477037581 10-11 24.019767216358087 28.293096508082407 27.77117077916681 19.9159654963927 12-13 25.236721455207995 25.80935086925186 28.523483917282245 20.4304437582579 14-15 22.35113678402 26.64434818461679 27.43576204700978 23.568752984353434 16-17 20.706996527760094 30.01954599113657 27.99187603828529 21.281581442818045 18-19 20.683207822151818 28.081339231401387 31.257906837741604 19.97754610870519 20-21 21.66738746791789 27.053921977050205 31.47867063199025 19.80001992304165 22-23 21.281943785781685 27.158064395217213 31.27692029392928 20.283071525071826 24-25 21.056488336877738 27.414352176767792 31.358592142037455 20.17056734431701 26-27 20.940006046496087 27.54447882381798 31.29224703929489 20.22326809039104 28-29 20.682775473948737 27.53416271178844 31.45837980888236 20.324682005380467 30-31 21.010896753388998 27.239010275246184 31.499696887879082 20.25039608348574 32-33 20.889873495080362 27.401373016358043 31.286744484507732 20.422009004053862 34-35 21.151497100650925 27.366314583448776 31.230218746842333 20.251969569057966 36-37 21.212599692556257 27.274396089005883 31.057930428883008 20.45507378955486 38-39 20.952620185285014 27.644520393615547 31.093342101714033 20.309517319385414 40-41 21.19548465183784 27.351353355595382 31.085465364898425 20.367696627668355 42-43 21.061144930896138 27.504014302774383 30.772803704770592 20.66203706155889 44-45 21.18185296141609 27.632814635817795 30.472083594597006 20.71324880816911 46-47 21.384118134534322 27.87796882524684 29.9021113728609 20.835801667357938 48-49 21.411826251060322 28.143570951883895 29.508608487971582 20.935994309084197 50-51 21.38364331442713 28.14670976211265 29.421727617822217 21.047919305638004 52-53 21.563411174486532 28.399584179001042 29.110607122696734 20.926397523815695 54-55 21.42862090151578 28.30437361781324 29.138452537519697 21.12855294315128 56-57 21.520344466316164 28.474511843423215 28.929499257169645 21.07564443309098 58-59 21.673281175072407 28.232033776975967 29.069450504857908 21.025234543093717 60-61 21.72815488491903 28.240870983217338 29.262192308514983 20.768781823348647 62-63 21.568389389631232 28.18455086175532 29.365907139920804 20.88115260869264 64-65 21.51507928747209 28.21937287113545 29.248989553284765 21.016558288107696 66-67 21.607284218057192 28.388091541224803 28.85086192180185 21.153762318916158 68-69 21.47938144224891 28.333254904405326 28.94732285400628 21.240040799339486 70-71 21.562237589578594 28.269717883205285 29.04204230020343 21.126002227012695 72-73 21.6051716824674 28.25136062058234 28.88235771088957 21.261109986060696 74-75 21.478575980260338 28.56917600927008 28.617742879167906 21.334505131301672 76-77 21.43370799518088 28.492872570018385 28.763408112236394 21.310011322564336 78-79 21.39416862163068 28.43815098264318 28.67227808399605 21.49540231173009 80-81 21.499548476032764 28.674674791721454 28.488402057392943 21.337374674852835 82-83 21.56014877146606 28.550724962449607 28.561094228515397 21.32803203756893 84-85 21.39739058746311 28.49550848343251 28.477785647803255 21.629315281301125 86-87 21.29514420232816 28.58258897651141 28.603347038951004 21.518919782209423 88-89 21.493798502514313 28.426947408734556 28.484434888096 21.594819200655138 90-91 21.35436984675871 28.48367004996002 28.552137178710208 21.609822924571063 92-93 21.484315319224354 28.42996335250248 28.459440244615426 21.62628108365774 94-95 21.339331403781532 28.424241461244364 28.380789877675543 21.855637257298564 96-97 21.40274968272108 28.392969547335163 28.31490772938298 21.889373040560773 98-99 21.202589406558342 28.317162783534638 28.255031041425777 22.22521676848124 100-101 21.240190787927084 28.570846538923337 28.129526212153994 22.059436460995585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1523.0 1 1063.5 2 651.5 3 727.5 4 646.5 5 658.5 6 1116.0 7 2365.5 8 3914.0 9 4631.5 10 4144.0 11 3505.5 12 3506.0 13 3661.5 14 3929.5 15 4267.0 16 4487.5 17 4814.0 18 5226.0 19 5607.5 20 6169.0 21 7021.5 22 8224.0 23 9865.5 24 11802.0 25 14400.5 26 18510.5 27 22922.5 28 26037.5 29 30222.0 30 36255.5 31 41590.0 32 47329.5 33 54715.0 34 63280.0 35 69928.5 36 77234.5 37 84685.5 38 86470.0 39 86258.0 40 86432.0 41 86943.0 42 86974.0 43 88141.0 44 90432.5 45 90868.5 46 89525.0 47 88866.0 48 91819.5 49 90314.0 50 83357.5 51 77396.5 52 72124.0 53 67990.0 54 62798.0 55 56041.5 56 49878.5 57 44061.5 58 38785.0 59 34030.0 60 28826.0 61 23135.0 62 17664.5 63 13033.0 64 9560.5 65 7062.0 66 4959.0 67 3686.5 68 2564.5 69 1531.0 70 1020.5 71 653.5 72 423.0 73 306.5 74 224.0 75 162.0 76 121.5 77 72.0 78 33.5 79 20.0 80 10.5 81 6.0 82 4.0 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022082178296885517 2 0.006477982194104792 3 0.007252080695287126 4 4.074202637801756E-5 5 0.004114944664179773 6 0.0 7 0.0 8 1.2222607913405268E-4 9 4.074202637801756E-5 10-11 0.0 12-13 4.074202637801756E-5 14-15 0.0 16-17 2.037101318900878E-5 18-19 0.004277912769691843 20-21 9.574376198834125E-4 22-23 0.0 24-25 0.005276092415953274 26-27 0.006274272062214704 28-29 0.00898361681635287 30-31 0.030780600928592267 32-33 7.333564748043161E-4 34-35 0.005052011270874177 36-37 0.01468750050927533 38-39 0.007944695143713423 40-41 0.008820648710840802 42-43 0.00484830113898409 44-45 0.0020778433452788955 46-47 0.0018741332133888075 48-49 0.0012222607913405267 50-51 0.0028926838728392467 52-53 0.0018945042265778164 54-55 9.778086330724215E-4 56-57 1.8333911870107902E-4 58-59 6.111303956702634E-4 60-61 2.037101318900878E-5 62-63 4.074202637801756E-5 64-65 0.004400138848825896 66-67 4.4816229015819315E-4 68-69 6.722434352372897E-4 70-71 0.002403779556303036 72-73 0.010470700779150513 74-75 0.004889043165362107 76-77 0.003137136031107352 78-79 0.010002167475803311 80-81 1.0185506594504389E-4 82-83 0.004053831624612747 84-85 3.055651978351317E-4 86-87 0.0 88-89 8.352115407493599E-4 90-91 2.2408114507909658E-4 92-93 1.2222607913405268E-4 94-95 4.889043165362107E-4 96-97 1.2222607913405268E-4 98-99 2.4445215826810536E-4 100-101 6.111303956702634E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2454468.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.400538536044195 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31594999856318 38.94195801742444 2 14.363709339698506 15.05332209550819 3 4.941266421611909 7.767750646276081 4 2.2032251459996965 4.618007366661548 5 1.1571924316747626 3.0318753304796044 6 0.6651366694970383 2.091211180902939 7 0.44654470016469566 1.6379427938332507 8 0.3089481081032406 1.2951237795441444 9 0.22089686156774913 1.041760305636485 >10 1.1988867768139633 11.742468629446607 >50 0.11121141067501089 4.0433659994897 >100 0.06361120859822351 5.948030626665892 >500 0.002643443615750226 0.8655329788447463 >1k 5.442383914770454E-4 0.5030643079670377 >5k 7.774834163957792E-5 0.3478835540760071 >10k+ 1.5549668327915583E-4 1.0707023872434174 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14446 0.5885593130568416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11835 0.4821818821838378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.34789616324189193 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2924 0.11912968512932334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.037101318900878E-5 16-17 0.0 0.0 0.0 8.148405275603512E-5 4.074202637801756E-5 18-19 0.0 0.0 0.0 1.4259709232306147E-4 4.074202637801756E-5 20-21 0.0 0.0 0.0 2.2408114507909658E-4 4.074202637801756E-5 22-23 0.0 0.0 0.0 3.66678237402158E-4 8.148405275603512E-5 24-25 0.0 6.111303956702634E-5 0.0 5.50017356103237E-4 1.2222607913405268E-4 26-27 0.0 1.2222607913405268E-4 0.0 8.759535671273775E-4 1.2222607913405268E-4 28-29 0.0 1.2222607913405268E-4 0.0 0.0014055999100416059 1.2222607913405268E-4 30-31 0.0 1.2222607913405268E-4 0.0 0.0037075244003995974 1.2222607913405268E-4 32-33 0.0 1.2222607913405268E-4 0.0 0.0072928227216651435 1.6296810551207023E-4 34-35 0.0 1.2222607913405268E-4 0.0 0.012650399190374452 1.6296810551207023E-4 36-37 0.0 1.2222607913405268E-4 0.0 0.021634016006727323 1.6296810551207023E-4 38-39 0.0 1.2222607913405268E-4 0.0 0.03440664127623583 1.6296810551207023E-4 40-41 0.0 1.2222607913405268E-4 0.0 0.0489719157063771 1.6296810551207023E-4 42-43 0.0 2.4445215826810536E-4 0.0 0.06518724220482809 1.6296810551207023E-4 44-45 0.0 2.4445215826810536E-4 0.0 0.08417302649698427 1.6296810551207023E-4 46-47 4.074202637801756E-5 2.4445215826810536E-4 0.0 0.10861824232379481 1.6296810551207023E-4 48-49 4.074202637801756E-5 3.055651978351317E-4 0.0 0.13689320863013898 1.6296810551207023E-4 50-51 4.074202637801756E-5 3.2593621102414047E-4 0.0 0.1691608935215289 1.6296810551207023E-4 52-53 4.074202637801756E-5 3.2593621102414047E-4 0.0 0.20148969145248585 1.6296810551207023E-4 54-55 4.074202637801756E-5 3.2593621102414047E-4 0.0 0.23944088902360916 1.6296810551207023E-4 56-57 4.074202637801756E-5 3.2593621102414047E-4 0.0 0.28965543653451586 1.6296810551207023E-4 58-59 4.074202637801756E-5 3.4630722421314926E-4 0.0 0.345696093817479 1.6296810551207023E-4 60-61 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.40574984069867687 1.6296810551207023E-4 62-63 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.47044817858696875 1.6296810551207023E-4 64-65 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.5408300291549941 1.6296810551207023E-4 66-67 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.6232104064913456 1.6296810551207023E-4 68-69 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.7210931248645327 1.6296810551207023E-4 70-71 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.8286317034893101 1.6296810551207023E-4 72-73 4.074202637801756E-5 3.6667823740215805E-4 0.0 0.9412834064245287 1.6296810551207023E-4 74-75 4.074202637801756E-5 3.6667823740215805E-4 0.0 1.0607186567516873 1.6296810551207023E-4 76-77 4.074202637801756E-5 3.6667823740215805E-4 0.0 1.1911135121745322 1.6296810551207023E-4 78-79 4.074202637801756E-5 3.6667823740215805E-4 0.0 1.34344794880194 1.6296810551207023E-4 80-81 8.148405275603512E-5 3.6667823740215805E-4 0.0 1.5131792306927612 1.6296810551207023E-4 82-83 8.148405275603512E-5 3.6667823740215805E-4 0.0 1.6876365876434323 1.6296810551207023E-4 84-85 8.148405275603512E-5 3.6667823740215805E-4 0.0 1.8726257584128212 1.6296810551207023E-4 86-87 8.148405275603512E-5 3.6667823740215805E-4 0.0 2.069389374805457 1.6296810551207023E-4 88-89 1.2222607913405268E-4 3.6667823740215805E-4 0.0 2.2826127698548118 1.6296810551207023E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8770 0.0 42.36996 1 GTATCAA 14265 0.0 36.508133 1 TATCAAC 18645 0.0 27.846836 2 ATCAACG 18695 0.0 27.77123 3 TCAACGC 18900 0.0 27.491219 4 CAACGCA 19245 0.0 26.949036 5 AACGCAG 19815 0.0 26.220684 6 ACGCAGA 22520 0.0 22.881376 7 CGCAGAG 22565 0.0 22.772604 8 GAGTACT 13150 0.0 21.850615 12-13 AGTACTT 13720 0.0 21.687078 12-13 GCAGAGT 23680 0.0 21.379438 9 GTACATG 12570 0.0 21.282566 1 AGAGTAC 20455 0.0 20.745115 10-11 GTACTTT 14015 0.0 20.43506 14-15 TACATGG 12735 0.0 20.366285 2 TAGGCCG 145 6.1540224E-4 19.652678 5 ACATGGG 12645 0.0 19.646414 3 TACTTTT 15965 0.0 18.4002 14-15 CAGAGTA 23275 0.0 18.394873 10-11 >>END_MODULE