FastQCFastQC Report
Thu 26 May 2016
SRR936805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485408
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158510.6377624921139708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109660.4412152853776925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77390.31137744788783167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77800.044.580371
GTATCAA136700.040.212891
ATCAACG169450.032.0483063
TCAACGC170600.031.8044224
TATCAAC173000.031.6927662
CAACGCA173400.031.3182535
AACGCAG178100.030.5451286
ACGCAGA202000.026.6478067
CGCAGAG201750.026.610188
GTACATG132550.025.485331
TACATGG132050.024.970172
GCAGAGT213150.024.9190049
CATTCCG4500.024.2822829
ACATGGG129900.024.0301573
GAGTACT116200.023.30396712-13
GTACTTT125900.021.60175314-15
AGTACTT123150.021.39085812-13
AGAGTAC188850.021.14437910-11
CATGGGG49700.020.9357324
CATGGGA82450.020.226324
Produced by FastQC (version 0.11.5)