##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936805_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2485408 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2672402277614 33.0 31.0 34.0 31.0 34.0 2 32.418192103670705 34.0 31.0 34.0 31.0 34.0 3 32.433907028544205 34.0 31.0 34.0 31.0 34.0 4 35.98997227014639 37.0 35.0 37.0 35.0 37.0 5 35.99704756724047 37.0 35.0 37.0 35.0 37.0 6 36.0986695142206 37.0 37.0 37.0 35.0 37.0 7 36.06528867694962 37.0 36.0 37.0 35.0 37.0 8 36.03761112863562 37.0 36.0 37.0 35.0 37.0 9 37.73557057835173 39.0 38.0 39.0 35.0 39.0 10-11 37.64074168104392 39.0 37.5 39.0 35.0 39.0 12-13 37.56679386241615 39.0 37.0 39.0 35.0 39.0 14-15 38.83942073092225 40.0 38.0 41.0 35.5 41.0 16-17 38.76514701006837 40.0 38.0 41.0 35.0 41.0 18-19 38.73716770043389 40.0 38.0 41.0 35.0 41.0 20-21 38.646780930937695 40.0 38.0 41.0 35.0 41.0 22-23 38.52638560751394 40.0 38.0 41.0 34.0 41.0 24-25 38.660280123022055 40.0 38.0 41.0 35.0 41.0 26-27 38.62230929489243 40.0 38.0 41.0 35.0 41.0 28-29 38.53935691846168 40.0 38.0 41.0 35.0 41.0 30-31 38.35546638620299 40.0 38.0 41.0 34.0 41.0 32-33 38.14546263631565 40.0 38.0 41.0 34.0 41.0 34-35 37.97718241833937 40.0 38.0 41.0 34.0 41.0 36-37 37.81331012855837 40.0 37.0 41.0 33.5 41.0 38-39 37.669285887870316 40.0 37.0 41.0 33.0 41.0 40-41 37.49819224851614 40.0 37.0 41.0 33.0 41.0 42-43 37.27612367868777 40.0 37.0 41.0 33.0 41.0 44-45 36.99793776313587 40.0 37.0 41.0 31.5 41.0 46-47 36.62572865300184 39.5 36.0 41.0 31.0 41.0 48-49 36.355399998712485 39.0 36.0 41.0 30.0 41.0 50-51 35.66817540621097 38.5 35.5 40.0 29.0 40.5 52-53 35.85989845530392 39.0 35.5 40.0 30.0 40.5 54-55 36.27069076787393 39.0 36.0 41.0 30.0 41.0 56-57 36.199235296579076 39.0 36.0 41.0 30.0 41.0 58-59 35.98448343290116 39.0 35.5 41.0 29.5 41.0 60-61 35.73582888604205 39.0 35.0 41.0 29.0 41.0 62-63 35.424495696481216 38.5 35.0 40.0 28.0 41.0 64-65 35.11047401472917 38.0 35.0 40.0 28.0 41.0 66-67 34.75530516518817 37.5 34.5 40.0 27.5 41.0 68-69 34.369701071212454 37.0 34.0 39.5 26.5 41.0 70-71 33.98290783645985 36.5 34.0 39.0 26.0 41.0 72-73 33.57827628300866 36.0 34.0 39.0 26.0 40.5 74-75 33.13433890934607 35.5 33.5 38.5 25.5 40.0 76-77 32.69960948061646 35.0 33.0 37.0 24.5 39.0 78-79 32.27685132581854 35.0 33.0 37.0 23.5 39.0 80-81 31.832511804902857 35.0 32.0 36.0 22.0 39.0 82-83 31.412039190346214 35.0 32.0 36.0 20.5 37.5 84-85 31.025299669108655 35.0 31.5 35.5 19.5 37.0 86-87 30.694396050869717 34.5 31.0 35.0 18.0 36.5 88-89 30.355885633264236 34.0 31.0 35.0 14.0 36.0 90-91 29.994991164428537 34.0 31.0 35.0 7.0 36.0 92-93 29.657287053071364 34.0 30.5 35.0 2.0 35.5 94-95 29.290108907672302 34.0 30.0 35.0 2.0 35.0 96-97 28.83563845453141 34.0 29.5 35.0 2.0 35.0 98-99 28.255478577360336 34.0 29.0 35.0 2.0 35.0 100-101 25.650859738119458 31.5 23.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1925679252386132 1101 2 0.2682716472428197 1101 3 0.16016684519431124 1101 4 0.09936880357908251 1101 5 0.17487428141193817 1101 6 0.07561795422559925 1101 7 0.06487092678467832 1101 8 0.05254376157706275 1101 9 0.1689491956322584 1101 10-11 0.2503367452554741 1101 12-13 0.29332862761986433 1101 14-15 0.27376275037796205 1101 16-17 0.32488517284540563 1101 18-19 0.402316673047153 1101 20-21 0.4296610819079021 1101 22-23 0.3196730822369247 1101 24-25 0.3882470297118985 1101 26-27 0.3500951381878181 1101 28-29 0.31502531203140194 1101 30-31 0.3406863390716879 1101 32-33 0.3989020320508274 1101 34-35 0.33907939252039654 1101 36-37 0.2638460802168865 1101 38-39 0.42392203775116144 1101 40-41 0.43454984430544386 1101 42-43 0.4455202103407956 1101 44-45 0.3084238465748115 1101 46-47 0.3268783923285241 1101 48-49 0.3487055432107553 1101 50-51 0.39913904754002516 1101 52-53 0.386977234635296 1101 54-55 0.47007794996201113 1101 56-57 0.36285128797998567 1101 58-59 0.3797583933620885 1101 60-61 0.5437389659817669 1101 62-63 0.5524685321102893 1101 64-65 0.53389322018689 1101 66-67 0.53595281571301 1101 68-69 0.4148454585364121 1101 70-71 0.3767411065972226 1101 72-73 0.28376665716039184 1101 74-75 0.34620212284563223 1101 76-77 0.4299110938678439 1101 78-79 0.41913223610436035 1101 80-81 0.3890881333503877 1101 82-83 0.3995866971097968 1101 84-85 0.23607818667654357 1101 86-87 0.3731138014633828 1101 88-89 0.4301117304512694 1101 90-91 0.41037148637560605 1101 92-93 0.4769270005131574 1101 94-95 0.5346082445806744 1101 96-97 0.5834765068472159 1101 98-99 0.4556941856474701 1101 100-101 0.6343032823035948 1102 1 0.23095729152537814 1102 2 0.2474580227977441 1102 3 0.13309022211364407 1102 4 0.12899366403755863 1102 5 0.14482781083897578 1102 6 0.1425422715177831 1102 7 0.11036224275861173 1102 8 0.18486806906167885 1102 9 0.19320562319614965 1102 10-11 0.23947613101315568 1102 12-13 0.20436860500476683 1102 14-15 0.1683914329040519 1102 16-17 0.3377536273743331 1102 18-19 0.362669284024129 1102 20-21 0.250382745964032 1102 22-23 0.31186989131526843 1102 24-25 0.039366217645117274 1102 26-27 0.1878235319675099 1102 28-29 0.14866262233425687 1102 30-31 0.12194453488269374 1102 32-33 0.3074515790276564 1102 34-35 0.19246251369292366 1102 36-37 0.10754370707346794 1102 38-39 0.23963151445962438 1102 40-41 0.319047826542203 1102 42-43 0.3027762254098363 1102 44-45 0.16601043443476726 1102 46-47 0.4352544607045985 1102 48-49 0.3799144992526564 1102 50-51 0.31117755090488686 1102 52-53 0.39226241857049615 1102 54-55 0.4925863801450987 1102 56-57 0.39974136531555615 1102 58-59 0.3662966962486891 1102 60-61 0.362735132375839 1102 62-63 0.5419758748069725 1102 64-65 0.5690121081238644 1102 66-67 0.43391191814730945 1102 68-69 0.5358213694144425 1102 70-71 0.4497374633735234 1102 72-73 0.34899836099485526 1102 74-75 0.43265394604399177 1102 76-77 0.40951636803269764 1102 78-79 0.2870568101241133 1102 80-81 0.43364778789316816 1102 82-83 0.4813910591706545 1102 84-85 0.37373175446848705 1102 86-87 0.34753427505361856 1102 88-89 0.43901936910578954 1102 90-91 0.4126952457901538 1102 92-93 0.3208020866899659 1102 94-95 0.40974028619938707 1102 96-97 0.49211230274591955 1102 98-99 0.4670720626547755 1102 100-101 0.43866453548023543 1103 1 0.09392097976702019 1103 2 0.11802530590642846 1103 3 0.12083023656860803 1103 4 0.14754233620759294 1103 5 0.20505812600336526 1103 6 0.12458461684155253 1103 7 0.1345350067805171 1103 8 0.08767214030733328 1103 9 0.0845612297277114 1103 10-11 0.100221647083508 1103 12-13 0.25584539468639633 1103 14-15 0.2578277006988472 1103 16-17 0.2812400501955423 1103 18-19 0.3083247513301046 1103 20-21 0.27769185576806166 1103 22-23 0.30753071696658907 1103 24-25 0.1310776114524259 1103 26-27 0.2556646397762776 1103 28-29 0.3136351645275113 1103 30-31 0.32911570328303696 1103 32-33 0.3136424101561346 1103 34-35 0.24654786927797545 1103 36-37 0.432180773433096 1103 38-39 0.36943720189191964 1103 40-41 0.26829229984885217 1103 42-43 0.3110231644693471 1103 44-45 0.4101132840462327 1103 46-47 0.597068949533309 1103 48-49 0.6445015376323511 1103 50-51 0.634548950959406 1103 52-53 0.5521024885283268 1103 54-55 0.6401201931322191 1103 56-57 0.682835374456964 1103 58-59 0.6845349385651716 1103 60-61 0.6947330114307846 1103 62-63 0.8155364278796071 1103 64-65 0.6249130744768863 1103 66-67 0.5621031898254714 1103 68-69 0.6264149369779233 1103 70-71 0.7047919025627039 1103 72-73 0.6599262321825208 1103 74-75 0.6634084604785073 1103 76-77 0.6832165351353723 1103 78-79 0.6582741210756282 1103 80-81 0.6502806059646566 1103 82-83 0.7085451425611353 1103 84-85 0.7366452867697113 1103 86-87 0.6863022349361785 1103 88-89 0.7191480653643829 1103 90-91 0.647803101627126 1103 92-93 0.5312258506887844 1103 94-95 0.6465792646085688 1103 96-97 0.37166155507965826 1103 98-99 0.3380051185271178 1103 100-101 0.5911089463604426 1104 1 0.20624538579258456 1104 2 0.20845630519987424 1104 3 0.193371101255714 1104 4 0.17546278223964862 1104 5 0.22866136599805742 1104 6 0.10297973422660078 1104 7 0.1564538233070465 1104 8 0.20460719451216391 1104 9 0.25910520588512753 1104 10-11 0.30559098254361317 1104 12-13 0.28081256687136147 1104 14-15 0.3494906651857832 1104 16-17 0.30955270869937834 1104 18-19 0.16644998391168997 1104 20-21 0.4187912146215709 1104 22-23 0.3000942537476803 1104 24-25 0.3054760960450764 1104 26-27 0.2944375270338426 1104 28-29 0.320620808813338 1104 30-31 0.23518184087953387 1104 32-33 0.3407624476642752 1104 34-35 0.38258535118374226 1104 36-37 0.25967900704254276 1104 38-39 0.16442155437658812 1104 40-41 0.2623357522366305 1104 42-43 0.29097811536286855 1104 44-45 0.09929944931481316 1104 46-47 0.10889679899584337 1104 48-49 0.10638315749854144 1104 50-51 0.04549540842423028 1104 52-53 -0.007654380722705412 1104 54-55 0.047072394849919874 1104 56-57 0.23909716747041898 1104 58-59 0.2961154933511523 1104 60-61 0.13903187323592903 1104 62-63 0.23889147155986734 1104 64-65 0.2502821312489161 1104 66-67 0.10475803793227811 1104 68-69 0.1386948348797361 1104 70-71 0.08531218039831856 1104 72-73 0.17495735508232002 1104 74-75 0.20650060295040618 1104 76-77 0.09200766826467088 1104 78-79 0.19681381199416847 1104 80-81 0.2364726777809345 1104 82-83 0.2795942064151262 1104 84-85 0.20748221982391968 1104 86-87 0.2566537589500726 1104 88-89 0.27834878798615037 1104 90-91 0.1356523018743374 1104 92-93 0.10815400326558589 1104 94-95 0.1776333120609479 1104 96-97 0.15347789636433973 1104 98-99 0.2338966826393083 1104 100-101 0.26002957108072877 1105 1 0.2050016679664708 1105 2 0.2566459200288591 1105 3 0.20552596320049332 1105 4 0.21002491217637242 1105 5 0.25825286361405375 1105 6 0.19198906677318206 1105 7 0.11796724223393795 1105 8 0.1548923933374553 1105 9 0.17600586445363575 1105 10-11 0.23259865001737268 1105 12-13 0.34653249891197646 1105 14-15 0.2952719437989302 1105 16-17 0.3558364764437627 1105 18-19 0.34097572641208274 1105 20-21 0.2387234247937826 1105 22-23 0.30832890353980247 1105 24-25 0.15205116675132757 1105 26-27 0.14075232413912175 1105 28-29 0.09256984266416168 1105 30-31 -0.055450089858858576 1105 32-33 0.1742343957655521 1105 34-35 -0.09129911785810663 1105 36-37 0.07195137361629378 1105 38-39 0.10061509959128045 1105 40-41 0.016888420761667078 1105 42-43 0.15722207057206816 1105 44-45 -0.013142985286144437 1105 46-47 -0.020627892236575462 1105 48-49 -0.06304605486436543 1105 50-51 0.037702036838311415 1105 52-53 0.022978635195521235 1105 54-55 0.11883881328169821 1105 56-57 0.17865858525787104 1105 58-59 0.21210307482441948 1105 60-61 0.15427441092896998 1105 62-63 0.13923593137769075 1105 64-65 0.1913691592804767 1105 66-67 0.0688886054135125 1105 68-69 0.12143581262676406 1105 70-71 0.09724746298593345 1105 72-73 0.07042147293284984 1105 74-75 0.11639676480498196 1105 76-77 0.0875859843368545 1105 78-79 0.08831829514335965 1105 80-81 0.16718606483066978 1105 82-83 0.053808129272404415 1105 84-85 0.11488034856694185 1105 86-87 -0.014623395979697307 1105 88-89 -1.333675879457985E-4 1105 90-91 -0.031363233153228975 1105 92-93 -0.09666902764169194 1105 94-95 -0.0994181865045789 1105 96-97 -0.12290214717849679 1105 98-99 -0.34500860829061963 1105 100-101 -0.2049386195853664 1106 1 0.05141269458013653 1106 2 0.16043756627698968 1106 3 0.17100880860647294 1106 4 0.16185758909163184 1106 5 0.2704283601625619 1106 6 0.16059367924452062 1106 7 0.11788859306368238 1106 8 0.19412320445239573 1106 9 0.17260428784027226 1106 10-11 0.2062114752770725 1106 12-13 0.177332799593799 1106 14-15 0.27718376900416786 1106 16-17 0.213199400014922 1106 18-19 0.16363545985189631 1106 20-21 0.3573604044236447 1106 22-23 0.16324916538103906 1106 24-25 0.22982536498353312 1106 26-27 0.19948207954019637 1106 28-29 0.24182895235556146 1106 30-31 0.2795707975457802 1106 32-33 0.25520485090224554 1106 34-35 0.23028372694287924 1106 36-37 0.17431478118233912 1106 38-39 0.14939913804467864 1106 40-41 0.17300399874231687 1106 42-43 0.19205987178326467 1106 44-45 0.16410123853729885 1106 46-47 0.21189232967508076 1106 48-49 0.23257311072843834 1106 50-51 0.1642587610398678 1106 52-53 0.116084506053582 1106 54-55 0.11004712165905062 1106 56-57 0.2724333521840805 1106 58-59 0.2759246077572399 1106 60-61 0.2135479043436206 1106 62-63 0.26982191307057946 1106 64-65 0.19653467040932782 1106 66-67 0.30313230289250015 1106 68-69 0.21076652331276335 1106 70-71 0.14574980128625725 1106 72-73 0.030982695261954518 1106 74-75 0.19905401777095477 1106 76-77 0.254544938544889 1106 78-79 0.32127600306454696 1106 80-81 0.1527770074712258 1106 82-83 0.1827723499498788 1106 84-85 0.24958633679376874 1106 86-87 0.37012456367932955 1106 88-89 0.3166704057572467 1106 90-91 0.22905362257393946 1106 92-93 0.10601858087903437 1106 94-95 0.18658347117793284 1106 96-97 0.1159372330660382 1106 98-99 0.08673071810948585 1106 100-101 -0.0844397720982073 1107 1 0.13370770842312396 1107 2 0.1596869553993443 1107 3 0.2319923005341451 1107 4 0.22834070494715064 1107 5 0.2608839479966889 1107 6 0.20845758176171358 1107 7 0.10852129132961608 1107 8 0.18967819264120322 1107 9 0.2254468335995483 1107 10-11 0.17308434908934345 1107 12-13 0.26949366953570575 1107 14-15 0.25218551327357375 1107 16-17 0.34363452431041 1107 18-19 0.30553886796263185 1107 20-21 0.3813527342385399 1107 22-23 0.40825231273308304 1107 24-25 0.3336373795111953 1107 26-27 0.1936723206787363 1107 28-29 0.2276677849294657 1107 30-31 0.3037419628940867 1107 32-33 0.3393809922103088 1107 34-35 0.2672129770757081 1107 36-37 0.4401383715251441 1107 38-39 0.34715788603492825 1107 40-41 0.39294256979081865 1107 42-43 0.4170338227489836 1107 44-45 0.4787605386367346 1107 46-47 0.5616300375594108 1107 48-49 0.4987626142902002 1107 50-51 0.27910682559087263 1107 52-53 0.3392101064847779 1107 54-55 0.3897966905753236 1107 56-57 0.46193978911321665 1107 58-59 0.37527244267805315 1107 60-61 0.4412218310045617 1107 62-63 0.4052579152511129 1107 64-65 0.5176648266421964 1107 66-67 0.46110177396101193 1107 68-69 0.4238250057907109 1107 70-71 0.2790436106249672 1107 72-73 0.3878582985683252 1107 74-75 0.4642174692315777 1107 76-77 0.3538820395001352 1107 78-79 0.3394997847786172 1107 80-81 0.45099359742836853 1107 82-83 0.3956190282858998 1107 84-85 0.3534698471991966 1107 86-87 0.38319872541412536 1107 88-89 0.4620748117434239 1107 90-91 0.47084443905303175 1107 92-93 0.3276416178092454 1107 94-95 0.3805272528000039 1107 96-97 0.3162167378622023 1107 98-99 0.3384084752627139 1107 100-101 0.07519020510744667 1108 1 -0.03293859010397426 1108 2 0.06275672500658658 1108 3 0.0029915614616555786 1108 4 0.06859396821408836 1108 5 0.1551348951151752 1108 6 0.12844687393976528 1108 7 -0.026954770286920393 1108 8 0.08709609449005029 1108 9 0.0851324659138939 1108 10-11 0.037149131412121505 1108 12-13 0.06209421540184934 1108 14-15 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-1.5419195288422358 2316 92-93 -1.9308612004457757 2316 94-95 -1.5407096624445487 2316 96-97 -1.4366902640512649 2316 98-99 -1.7261152786010214 2316 100-101 -2.000230801498784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3423.0 3 1026.0 4 928.0 5 997.0 6 1094.0 7 1347.0 8 1599.0 9 2047.0 10 2533.0 11 3535.0 12 5381.0 13 7319.0 14 8557.0 15 11575.0 16 16885.0 17 25088.0 18 22074.0 19 10637.0 20 9481.0 21 9372.0 22 9889.0 23 10605.0 24 12104.0 25 13595.0 26 16418.0 27 19526.0 28 23847.0 29 29964.0 30 38115.0 31 50347.0 32 68519.0 33 97365.0 34 144906.0 35 222479.0 36 359267.0 37 571493.0 38 551619.0 39 100411.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7125560655421 19.914601288224677 15.118992762036608 22.25384988419661 2 14.587617976488199 20.96855905212393 41.77342478376426 22.67039818762361 3 18.447645831983625 26.44751545509381 30.472419050638948 24.632419662283617 4 11.959682998196714 17.551884235506602 39.24396365739241 31.244469108904276 5 12.788527574415939 38.3746404098422 34.57270665413888 14.264125361602979 6 29.312790409366517 37.81967168245034 18.698539379751605 14.168998528431539 7 26.61130851539016 34.028482553530544 21.932330796811307 17.427878134267996 8 25.364483122277946 35.05816434143475 20.754486100936266 18.822866435351038 9 26.452109611159607 16.84320375474847 21.31883044833136 35.38585618576056 10-11 23.6968575519603 27.67914561600207 28.284104893680894 20.339891938356732 12-13 25.0807786303293 25.354266818215482 28.652727556661763 20.91222699479346 14-15 22.304979993665903 26.052460338443673 27.664637260192602 23.97792240769782 16-17 20.827264443053437 29.480470799004994 28.02301158204828 21.669253175893292 18-19 20.655435832152765 27.833769645358906 31.081351619605936 20.429442902882396 20-21 21.736459731040625 26.849037854264413 31.099798337811023 20.314704076883935 22-23 21.30173788639006 26.979176788963123 30.905772762014323 20.813312562632493 24-25 21.03896950409527 27.167462022200752 30.987783894246583 20.805784579457395 26-27 20.871423194323825 27.337128689865697 30.93587746546791 20.85557065034257 28-29 20.57472973225805 27.351375122995286 31.157645403009106 20.916249741737563 30-31 20.985608801452315 27.049442184140393 31.145731405065085 20.819217609342207 32-33 20.743808735586306 27.21587335946732 31.01826388299334 21.022054021953032 34-35 21.073961356816554 27.28216235093985 30.82663355604678 20.81724273619682 36-37 21.03411190436339 27.10309534692091 30.76517014510294 21.097622603612766 38-39 20.838542910385083 27.48441753894182 30.786130960870988 20.89090858980211 40-41 21.069446573539064 27.18236892388249 30.838933019823312 20.90925148275514 42-43 20.79542786697296 27.349109571500335 30.555428703858865 21.300033857667835 44-45 20.957831822177873 27.46934604219433 30.340206123348107 21.23261601227969 46-47 21.105186754046017 27.64586337534924 29.863366497573036 21.38558337303171 48-49 21.128967817291603 27.81587130940133 29.588722633713726 21.466438239593337 50-51 21.117850233231653 27.84150427023046 29.524208232502996 21.516437264034888 52-53 21.29570953510408 28.11840773800543 29.22627992733549 21.359602799555 54-55 21.135624539178735 27.9885610708254 29.307095817628948 21.56871857236692 56-57 21.255084185542547 28.2129296536618 29.014188041134574 21.517798119661077 58-59 21.36204816804821 27.94578459166438 29.202794580470933 21.489372659816482 60-61 21.450030166965455 27.97253246997537 29.3686423296341 21.20879503342508 62-63 21.241015357180792 27.94032796963971 29.50036855946361 21.318288113715884 64-65 21.138877064172934 27.95015486577897 29.40110116046734 21.509866909580754 66-67 21.20781927778717 28.277682545047767 28.92263614927127 21.5918620278938 68-69 21.12550006609534 28.083518759105715 29.192733415652384 21.59824775914656 70-71 21.156078948395717 28.003395024092352 29.247170712645175 21.593355314866752 72-73 21.249832243832056 27.975109109416458 29.106937590278974 21.66812105647251 74-75 21.066826400415138 28.344584649664572 28.88388726054274 21.70470168937755 76-77 21.072702176825274 28.141845301783892 29.01480510035516 21.770647421035676 78-79 21.104238558507802 28.11207581717758 28.941718562934494 21.84196706138012 80-81 21.063102463136477 28.429869995260297 28.768773906069338 21.73825363553389 82-83 21.250398457045524 28.381873987758112 28.726036229807484 21.64169132538888 84-85 21.191687867663926 28.21990900405884 28.636819472133396 21.951583656143836 86-87 21.00031427445858 28.34057452268309 28.840757815917257 21.818353386941073 88-89 21.19811106458946 28.125548895649615 28.776447339114853 21.89989270064607 90-91 21.13822037968716 28.195293781967322 28.82442341353834 21.842062424807178 92-93 21.295318634804897 28.12948437227391 28.806354806401895 21.7688421865193 94-95 21.11080529037679 28.010080817153003 28.832316076996 22.046797815474207 96-97 21.12934144050119 28.01031323825339 28.780425773196082 22.079919548049336 98-99 20.956995394498147 28.00904559440796 28.619953230622613 22.41400578047128 100-101 21.14235125611626 28.004194869435594 28.626663261504948 22.226790612943198 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1131.0 1 837.0 2 649.5 3 788.5 4 660.0 5 630.0 6 1183.5 7 2649.0 8 4675.5 9 5358.0 10 4338.5 11 3496.0 12 3444.0 13 3602.5 14 3860.0 15 3996.5 16 4086.0 17 4337.0 18 4570.5 19 4873.5 20 5290.5 21 6251.5 22 7499.5 23 8971.5 24 10950.0 25 13555.5 26 17470.5 27 21916.5 28 25162.0 29 28956.5 30 35112.5 31 40906.0 32 46652.5 33 54108.0 34 63142.0 35 70010.0 36 77284.0 37 85044.5 38 86987.5 39 86692.0 40 86648.0 41 87436.5 42 87225.0 43 88638.0 44 91961.5 45 92417.5 46 90784.5 47 89990.0 48 93578.0 49 92237.0 50 85565.0 51 80425.0 52 74598.0 53 70226.0 54 65685.0 55 59266.5 56 53198.0 57 47026.5 58 41312.5 59 36375.0 60 30931.5 61 24512.0 62 18283.0 63 13407.5 64 9796.0 65 7237.5 66 5147.5 67 3746.0 68 2542.5 69 1497.5 70 967.5 71 631.0 72 423.0 73 329.0 74 242.5 75 177.5 76 126.0 77 71.0 78 42.5 79 23.5 80 17.5 81 18.5 82 19.5 83 13.0 84 9.5 85 11.0 86 7.5 87 5.0 88 4.0 89 3.0 90 2.5 91 1.0 92 0.0 93 0.0 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023859261738917715 2 0.010984112065302759 3 0.012271627032664253 4 0.020157656207753415 5 0.05351234083096215 6 0.03959108524636599 7 0.015208770551957667 8 0.04297081203568991 9 0.02659523104456089 10-11 0.02132446664692477 12-13 0.03379726789323926 14-15 0.0179045050148708 16-17 0.016033584827923626 18-19 0.015067948602402504 20-21 0.01253315351040956 22-23 0.007101449741853249 24-25 0.0016697459732969396 26-27 1.0058710682511684E-4 28-29 0.0019513898724072669 30-31 0.0 32-33 4.425832700305141E-4 34-35 8.046968546009348E-5 36-37 0.0 38-39 6.035226409507011E-5 40-41 4.023484273004674E-5 42-43 1.4082194955516357E-4 44-45 1.2070452819014021E-4 46-47 0.0 48-49 2.011742136502337E-4 50-51 1.4082194955516357E-4 52-53 5.230529554906076E-4 54-55 0.001428336916916659 56-57 2.011742136502337E-4 58-59 0.0010461059109812152 60-61 0.0029572609406584354 62-63 0.002253151192882617 64-65 0.001207045281901402 66-67 0.010782937851652526 68-69 0.014544895646911894 70-71 0.02393973142437781 72-73 0.01619452419884381 74-75 0.017683213379855543 76-77 0.030477893368010406 78-79 0.019433429038612574 80-81 0.043272573356165266 82-83 0.03315351040955851 84-85 0.018588497341281592 86-87 0.01283491483088491 88-89 0.01225150961129923 90-91 0.02198834155197054 92-93 0.029673196513409467 94-95 0.011748574077173647 96-97 0.01796485727896587 98-99 0.026655583308655962 100-101 0.03800180895852914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2485408.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.17421598562926 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1386055008715 37.428107947893295 2 14.941156306822045 15.292039198407162 3 5.140996396116933 7.892593798686887 4 2.3425482259272234 4.795122754814096 5 1.2316140394257937 3.151344143225444 6 0.7179470839102121 2.204422748296433 7 0.47489400492155615 1.7011629864695337 8 0.3281018890201282 1.3432285547207201 9 0.2272516770110382 1.0466483762213394 >10 1.2679359053539265 12.072251528085431 >50 0.1180795521994889 4.187143021956832 >100 0.06772427200452627 6.264334556230889 >500 0.002280231151284944 0.7722400286833426 >1k 6.290292831121583E-4 0.45890983363502696 >5k 7.862866038901979E-5 0.31139879022371536 >10k+ 1.5725732077803958E-4 1.0790517324498479 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15851 0.6377624921139708 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10966 0.4412152853776925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7739 0.31137744788783167 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 2.011742136502337E-5 0.0 0.0 0.0 16-17 0.0 4.023484273004674E-5 0.0 6.035226409507011E-5 0.0 18-19 0.0 4.023484273004674E-5 0.0 8.046968546009348E-5 0.0 20-21 0.0 4.023484273004674E-5 0.0 1.0058710682511684E-4 0.0 22-23 0.0 4.023484273004674E-5 0.0 2.615264777453038E-4 2.011742136502337E-5 24-25 0.0 1.0058710682511684E-4 0.0 5.230529554906076E-4 4.023484273004674E-5 26-27 0.0 1.2070452819014021E-4 0.0 8.449316973309815E-4 4.023484273004674E-5 28-29 0.0 1.2070452819014021E-4 0.0 0.0016697459732969396 4.023484273004674E-5 30-31 0.0 1.2070452819014021E-4 0.0 0.003077965468848575 4.023484273004674E-5 32-33 0.0 1.2070452819014021E-4 0.0 0.006015108988141987 4.023484273004674E-5 34-35 0.0 1.2070452819014021E-4 0.0 0.011487047599428344 4.023484273004674E-5 36-37 0.0 1.8105679228521033E-4 0.0 0.019574250988167737 4.023484273004674E-5 38-39 0.0 2.8164389911032714E-4 0.0 0.031363059908071436 4.023484273004674E-5 40-41 0.0 3.218787418403739E-4 0.0 0.0446807928517169 4.023484273004674E-5 42-43 0.0 3.621135845704206E-4 0.0 0.057757116738982095 4.023484273004674E-5 44-45 0.0 4.023484273004674E-4 0.0 0.07580244370340805 4.023484273004674E-5 46-47 0.0 4.023484273004674E-4 0.0 0.09704644066487272 6.035226409507011E-5 48-49 0.0 4.023484273004674E-4 0.0 0.12201216057886674 1.2070452819014021E-4 50-51 0.0 4.023484273004674E-4 0.0 0.14939197105666352 1.2070452819014021E-4 52-53 0.0 4.023484273004674E-4 0.0 0.17926634178372325 1.4082194955516357E-4 54-55 0.0 4.023484273004674E-4 0.0 0.2158196964039707 1.6093937092018695E-4 56-57 0.0 4.023484273004674E-4 0.0 0.26072178089070286 1.6093937092018695E-4 58-59 0.0 4.2246584866549076E-4 0.0 0.3124235537988129 1.6093937092018695E-4 60-61 0.0 4.425832700305141E-4 0.0 0.3725142914161377 1.6093937092018695E-4 62-63 0.0 4.425832700305141E-4 0.0 0.43361090010171366 1.6093937092018695E-4 64-65 0.0 4.425832700305141E-4 0.0 0.4989925195380397 1.6093937092018695E-4 66-67 0.0 4.425832700305141E-4 0.0 0.5758410691524289 1.6093937092018695E-4 68-69 0.0 4.425832700305141E-4 0.0 0.6705538889389588 1.6093937092018695E-4 70-71 0.0 4.425832700305141E-4 0.0 0.7797512521083058 1.6093937092018695E-4 72-73 0.0 4.425832700305141E-4 0.0 0.8942193796752886 1.6093937092018695E-4 74-75 0.0 4.425832700305141E-4 0.0 1.012550052144356 1.6093937092018695E-4 76-77 0.0 4.425832700305141E-4 0.0 1.1447013930911947 1.8105679228521033E-4 78-79 0.0 4.425832700305141E-4 0.0 1.3001889428214604 2.011742136502337E-4 80-81 0.0 4.425832700305141E-4 0.0 1.4683705854330555 2.011742136502337E-4 82-83 0.0 4.425832700305141E-4 0.0 1.6462890599853224 2.011742136502337E-4 84-85 0.0 4.425832700305141E-4 0.0 1.8335219006295949 2.011742136502337E-4 86-87 0.0 4.425832700305141E-4 0.0 2.0363658602531256 2.011742136502337E-4 88-89 0.0 4.425832700305141E-4 0.0 2.2602124077817405 2.011742136502337E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7780 0.0 44.58037 1 GTATCAA 13670 0.0 40.21289 1 ATCAACG 16945 0.0 32.048306 3 TCAACGC 17060 0.0 31.804422 4 TATCAAC 17300 0.0 31.692766 2 CAACGCA 17340 0.0 31.318253 5 AACGCAG 17810 0.0 30.545128 6 ACGCAGA 20200 0.0 26.647806 7 CGCAGAG 20175 0.0 26.61018 8 GTACATG 13255 0.0 25.48533 1 TACATGG 13205 0.0 24.97017 2 GCAGAGT 21315 0.0 24.919004 9 CATTCCG 450 0.0 24.282282 9 ACATGGG 12990 0.0 24.030157 3 GAGTACT 11620 0.0 23.303967 12-13 GTACTTT 12590 0.0 21.601753 14-15 AGTACTT 12315 0.0 21.390858 12-13 AGAGTAC 18885 0.0 21.144379 10-11 CATGGGG 4970 0.0 20.935732 4 CATGGGA 8245 0.0 20.22632 4 >>END_MODULE