FastQCFastQC Report
Thu 26 May 2016
SRR936805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485408
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140900.5669089340663586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117770.4738457428317604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81380.3274311501371203No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.1183709073117975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.040.03311
GTATCAA147900.036.1337851
ATCAACG189900.028.0875573
TCAACGC191100.027.9360374
TATCAAC191450.027.837032
CAACGCA194700.027.3740165
AACGCAG199650.026.6953226
ACGCAGA227850.023.2245757
CGCAGAG227450.023.0983418
GAGTACT130200.021.99666412-13
GCAGAGT235650.021.8914249
AGTACTT136150.021.08769612-13
AGAGTAC202500.020.84222210-11
GTACTTT138700.020.54631814-15
GTACATG123200.020.281691
TACATGG125400.019.5072082
ACATGGG125050.018.6490633
CAGAGTA231100.018.5917510-11
CGGGTCG1152.0622228E-618.58548514-15
TACCGCG1300.00756372718.2699898
Produced by FastQC (version 0.11.5)