##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936805_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2485408 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.512735132420914 31.0 31.0 34.0 30.0 34.0 2 31.665701969254144 31.0 31.0 34.0 30.0 34.0 3 31.744341774066875 31.0 31.0 34.0 30.0 34.0 4 35.53247796740012 37.0 35.0 37.0 33.0 37.0 5 35.407564874660416 37.0 35.0 37.0 33.0 37.0 6 35.463008487942425 37.0 35.0 37.0 33.0 37.0 7 35.39758261017909 37.0 35.0 37.0 33.0 37.0 8 35.35169678378761 37.0 35.0 37.0 33.0 37.0 9 36.896575129717135 39.0 37.0 39.0 33.0 39.0 10-11 36.76110018958658 39.0 36.0 39.0 32.0 39.0 12-13 36.62523477030733 38.0 35.0 39.0 32.0 39.0 14-15 37.56550936506199 39.0 36.0 40.5 32.0 41.0 16-17 37.424625655023235 39.0 36.0 40.0 32.0 41.0 18-19 37.64869268949002 39.0 36.5 40.5 32.0 41.0 20-21 37.69428278978744 39.0 37.0 41.0 32.0 41.0 22-23 37.580101938997544 39.0 36.5 41.0 32.0 41.0 24-25 37.4285040926882 39.0 36.0 41.0 32.0 41.0 26-27 37.15587259717519 39.0 36.0 40.0 31.0 41.0 28-29 37.00985894468836 39.0 36.0 40.0 31.0 41.0 30-31 36.9376709980816 39.0 36.0 40.0 31.0 41.0 32-33 36.700485996665336 38.5 35.0 40.0 30.0 41.0 34-35 36.483379388816644 38.0 35.0 40.0 30.0 41.0 36-37 36.252623311746 38.0 35.0 40.0 30.0 41.0 38-39 36.041433237520764 38.0 35.0 40.0 30.0 41.0 40-41 35.77970779847816 38.0 35.0 40.0 29.0 41.0 42-43 35.42631934072796 38.0 34.0 40.0 27.5 41.0 44-45 35.05942686271228 38.0 34.0 40.0 26.5 41.0 46-47 34.598819389009776 38.0 33.0 40.0 24.5 41.0 48-49 34.13206725012553 38.0 33.0 40.0 24.0 41.0 50-51 33.76377641015077 37.5 33.0 40.0 23.0 41.0 52-53 33.44667032535503 37.0 32.5 40.0 22.0 41.0 54-55 33.095367843026175 37.0 32.0 39.5 19.5 40.0 56-57 32.71679921365023 36.5 31.0 39.0 18.5 40.0 58-59 32.25714389750093 36.0 31.0 39.0 16.5 40.0 60-61 31.83064128706434 36.0 30.0 39.0 13.5 40.0 62-63 31.35890887934697 35.0 29.5 38.0 11.0 40.0 64-65 30.8740906925543 35.0 29.0 38.0 8.0 40.0 66-67 30.354616827498745 34.0 28.0 38.0 8.0 40.0 68-69 29.925621467380807 34.0 28.0 37.5 7.0 39.0 70-71 29.340813460003346 34.0 27.5 37.0 3.5 39.0 72-73 28.705931581454635 33.0 26.0 36.0 2.0 39.0 74-75 28.042998574077174 32.5 26.0 35.5 2.0 38.0 76-77 26.97037468294944 31.0 25.0 34.0 2.0 36.5 78-79 27.681824271910287 32.0 26.0 35.0 2.0 37.0 80-81 27.37881929244615 32.0 26.0 35.0 2.0 37.0 82-83 26.874193291403262 32.0 25.0 35.0 2.0 36.0 84-85 26.30896396084667 32.0 24.0 34.5 2.0 36.0 86-87 25.73381553451184 31.0 22.5 34.0 2.0 35.0 88-89 25.086368515752746 31.0 20.0 34.0 2.0 35.0 90-91 24.308974220731564 31.0 17.5 34.0 2.0 35.0 92-93 23.474082524881226 30.0 8.5 34.0 2.0 35.0 94-95 22.46628963936706 29.5 2.0 34.0 2.0 35.0 96-97 21.30112641465707 29.0 2.0 34.0 2.0 35.0 98-99 19.922362445119674 28.0 2.0 33.0 2.0 34.0 100-101 16.351524981009156 20.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1646805988208726 1101 2 0.17895505490850283 1101 3 0.23336854152074338 1101 4 0.254064947356305 1101 5 0.23989955390447193 1101 6 0.26732120014917626 1101 7 0.1936522045718405 1101 8 0.300710991855901 1101 9 0.37367487845831704 1101 10-11 0.37384712345090776 1101 12-13 0.33257794731424184 1101 14-15 0.44216173367067313 1101 16-17 0.5103767803544201 1101 18-19 0.376633825904392 1101 20-21 0.3723649517282226 1101 22-23 0.4599449700767835 1101 24-25 0.445039477830214 1101 26-27 0.5319145473621703 1101 28-29 0.42839965510901834 1101 30-31 0.4732107652971962 1101 32-33 0.5444441357098668 1101 34-35 0.5176010053658757 1101 36-37 0.5265160025945761 1101 38-39 0.5601835032233495 1101 40-41 0.5959294749775239 1101 42-43 0.5993663000099048 1101 44-45 0.623251885412138 1101 46-47 0.539309151693601 1101 48-49 0.5276564656061637 1101 50-51 0.44881073839253105 1101 52-53 0.33310843424693104 1101 54-55 0.38443385709229716 1101 56-57 0.4683377780571121 1101 58-59 0.43799702291624953 1101 60-61 0.5852319331599709 1101 62-63 0.5611630328521713 1101 64-65 0.5227558653510229 1101 66-67 0.2316708862236112 1101 68-69 0.21178294925114471 1101 70-71 0.2615068119039279 1101 72-73 0.45977157968890836 1101 74-75 0.6402668289189108 1101 76-77 0.40223486458770097 1101 78-79 0.4915578381207517 1101 80-81 0.8200815049653833 1101 82-83 0.8545496034559328 1101 84-85 0.7881155595281477 1101 86-87 0.25148936981105763 1101 88-89 0.42530479229919393 1101 90-91 0.7372002831867732 1101 92-93 0.8164263280739128 1101 94-95 0.6262433409225885 1101 96-97 0.5781208641595086 1101 98-99 0.6960958400445634 1101 100-101 0.7229721072856812 1102 1 0.33790512582197607 1102 2 0.3387974414940551 1102 3 0.3042998856481738 1102 4 0.27814218485237774 1102 5 0.28091354122962997 1102 6 0.15474795346860049 1102 7 0.1480834664887567 1102 8 0.2448461517048628 1102 9 0.41958641994866497 1102 10-11 0.35253625522138066 1102 12-13 0.39778288225254954 1102 14-15 0.5295974291860119 1102 16-17 0.4939539471782979 1102 18-19 0.38514800793866044 1102 20-21 0.423449409326345 1102 22-23 0.3545914793031173 1102 24-25 0.45072279140418203 1102 26-27 0.4625124918109478 1102 28-29 0.4541681214647326 1102 30-31 0.4707546613831113 1102 32-33 0.5029050395639558 1102 34-35 0.49763642800001406 1102 36-37 0.54830761481481 1102 38-39 0.661684061389451 1102 40-41 0.5407080690912522 1102 42-43 0.6772030962807207 1102 44-45 0.5394428561019282 1102 46-47 0.5875093761138785 1102 48-49 0.6171099820525967 1102 50-51 0.7086336829134154 1102 52-53 0.6860229266619271 1102 54-55 0.7245051062016827 1102 56-57 0.724235848561996 1102 58-59 0.8537847871076565 1102 60-61 0.9574642802556319 1102 62-63 0.9665312926719984 1102 64-65 1.0492829760698292 1102 66-67 0.8364303844221901 1102 68-69 0.6184409621798288 1102 70-71 0.6970012143777389 1102 72-73 1.037625337286169 1102 74-75 1.125651069308212 1102 76-77 1.0327918480100244 1102 78-79 1.0215770205732788 1102 80-81 1.3216631775021668 1102 82-83 1.1266779326210532 1102 84-85 1.127802813437473 1102 86-87 1.1810364620108693 1102 88-89 1.3751388424504896 1102 90-91 1.3345679891673718 1102 92-93 1.2330903697733895 1102 94-95 1.1662783597156228 1102 96-97 1.3254865220887844 1102 98-99 1.4859083135424882 1102 100-101 1.402837916655372 1103 1 0.21076141637088597 1103 2 0.29060363802627975 1103 3 0.1858586317359503 1103 4 0.21720934377084689 1103 5 0.17933233807659832 1103 6 0.272647774575276 1103 7 0.21428805108445914 1103 8 0.14602066901391453 1103 9 0.2735255806786867 1103 10-11 0.25425300508901216 1103 12-13 0.3481711166514856 1103 14-15 0.5346463843543745 1103 16-17 0.4996494455512561 1103 18-19 0.4370790573448886 1103 20-21 0.4277584786857247 1103 22-23 0.3277093804952287 1103 24-25 0.2617140144573611 1103 26-27 0.4326244640910488 1103 28-29 0.3078646713986757 1103 30-31 0.3281483553532638 1103 32-33 0.45341386529570826 1103 34-35 0.5109285519635662 1103 36-37 0.2724758773696152 1103 38-39 0.16077982633152743 1103 40-41 0.307253017154153 1103 42-43 0.4526988232002438 1103 44-45 0.2680380944637477 1103 46-47 0.48140551241555585 1103 48-49 0.4340097805376857 1103 50-51 0.5510699957228766 1103 52-53 0.5529921208604733 1103 54-55 0.4227788506979522 1103 56-57 0.43283331937730196 1103 58-59 0.5395310286682644 1103 60-61 0.22547717501658937 1103 62-63 0.5071825110043484 1103 64-65 0.42591033068558204 1103 66-67 0.36726463423442013 1103 68-69 0.226521244131618 1103 70-71 0.2371395625129793 1103 72-73 0.4694082884251394 1103 74-75 0.75996724961459 1103 76-77 0.2915318919415988 1103 78-79 0.4904706262278502 1103 80-81 0.6343824383658436 1103 82-83 0.6059586735853841 1103 84-85 0.5611735599795118 1103 86-87 0.45878806374482295 1103 88-89 0.2044391545141231 1103 90-91 0.3518397984897952 1103 92-93 0.5734390415321222 1103 94-95 0.384488976277229 1103 96-97 0.3744798500919515 1103 98-99 0.2826441100999446 1103 100-101 0.463616633901772 1104 1 0.17683278346700249 1104 2 0.2437037149114012 1104 3 0.1776988090471363 1104 4 0.11177591005516518 1104 5 0.2454355083992681 1104 6 0.11988409058131566 1104 7 0.17629057239180668 1104 8 0.21183203653626492 1104 9 0.2930870240205863 1104 10-11 0.2556390683905505 1104 12-13 0.16622797229597808 1104 14-15 0.1933182553840993 1104 16-17 0.20913762671817437 1104 18-19 0.15053526479372437 1104 20-21 0.22242274972790455 1104 22-23 0.17808585528789678 1104 24-25 0.2310218516576228 1104 26-27 0.21594940945998786 1104 28-29 0.15481041411057106 1104 30-31 0.274084919391683 1104 32-33 0.1365697125812062 1104 34-35 0.17322254820290794 1104 36-37 0.1908342079297327 1104 38-39 0.3198062049768069 1104 40-41 0.289926406214164 1104 42-43 0.1638910999342187 1104 44-45 0.25793565200873303 1104 46-47 0.3405545138106376 1104 48-49 0.05414769834437294 1104 50-51 0.42100135649113923 1104 52-53 0.32307446498777637 1104 54-55 0.24564143616912304 1104 56-57 0.4722730088204301 1104 58-59 0.4082832930088145 1104 60-61 0.6323797866516507 1104 62-63 0.5437258937201186 1104 64-65 0.4823364676919155 1104 66-67 0.5106521040910295 1104 68-69 0.28988794353047 1104 70-71 0.27207884949775263 1104 72-73 0.4007850402665305 1104 74-75 0.32477247124537456 1104 76-77 0.25401105181182615 1104 78-79 0.43281662238196006 1104 80-81 0.7726080314386401 1104 82-83 0.8059800669929373 1104 84-85 0.6215480511688689 1104 86-87 0.36067183114848334 1104 88-89 0.1752166614362416 1104 90-91 0.2621596176376322 1104 92-93 0.24195228987106177 1104 94-95 0.20700934919464942 1104 96-97 0.31123897015896773 1104 98-99 0.1866901690408298 1104 100-101 0.4375396274706347 1105 1 0.26374042788070184 1105 2 0.28510926018035576 1105 3 0.30271746057554694 1105 4 0.1605911988825568 1105 5 0.14488087976815223 1105 6 0.20262142280233775 1105 7 0.2038651615495013 1105 8 0.19522115489547076 1105 9 0.3709707198718064 1105 10-11 0.3710869424554346 1105 12-13 0.2795644085654061 1105 14-15 0.3264914399121466 1105 16-17 0.1793617333099604 1105 18-19 0.2795326264502549 1105 20-21 0.35873193441872786 1105 22-23 0.3163157235063707 1105 24-25 0.1498351396847042 1105 26-27 0.399119711479095 1105 28-29 0.24510331093031823 1105 30-31 0.22409204088434365 1105 32-33 0.2812636070881638 1105 34-35 0.49019976281757494 1105 36-37 0.3522547083332839 1105 38-39 0.2923910016073208 1105 40-41 0.3152954521376188 1105 42-43 0.3546322268934716 1105 44-45 0.3621718380085639 1105 46-47 0.36734531487682176 1105 48-49 0.2794992796548641 1105 50-51 0.2855899885541362 1105 52-53 0.21675312289161752 1105 54-55 0.22701322217166364 1105 56-57 0.24157055834871954 1105 58-59 0.273361527692515 1105 60-61 0.1946250237295324 1105 62-63 0.47345456484958603 1105 64-65 0.04940439655837636 1105 66-67 0.022141869488709887 1105 68-69 0.09664666396779964 1105 70-71 0.011207127927768568 1105 72-73 0.13642675457937514 1105 74-75 0.2827429475714993 1105 76-77 0.014371975842458085 1105 78-79 0.27550454500863353 1105 80-81 0.38893591852567866 1105 82-83 0.42609039926143666 1105 84-85 0.43777242227324464 1105 86-87 0.24210364116062522 1105 88-89 -0.037439587504010774 1105 90-91 0.03771687443865801 1105 92-93 0.12194920389090669 1105 94-95 -0.014352076731650953 1105 96-97 -0.0074300228619677 1105 98-99 -0.16258386260572877 1105 100-101 0.19795989727347063 1106 1 0.16024870721835427 1106 2 0.0729614420898379 1106 3 0.10759718784442285 1106 4 0.21801946176438491 1106 5 0.18439679412525578 1106 6 0.1541909274940636 1106 7 0.12573099211498828 1106 8 0.21324315851333608 1106 9 0.20761185580781927 1106 10-11 0.18849684378097464 1106 12-13 0.28345867685587933 1106 14-15 0.248288074332649 1106 16-17 0.17568072526959355 1106 18-19 -0.11304016940675155 1106 20-21 0.018116368537953065 1106 22-23 0.1554554830819015 1106 24-25 0.13039215730816522 1106 26-27 0.1626961463727099 1106 28-29 0.3523013737407581 1106 30-31 0.26957394855022443 1106 32-33 0.1959523227472033 1106 34-35 0.08291011909831525 1106 36-37 0.12932968519227472 1106 38-39 0.2276725656368157 1106 40-41 0.3107924090197258 1106 42-43 0.27600158457174473 1106 44-45 0.0383834133514398 1106 46-47 0.09398083949704272 1106 48-49 0.16384907788949477 1106 50-51 0.1188616881627027 1106 52-53 0.10460923725960924 1106 54-55 0.06944225642035207 1106 56-57 0.10246286343490141 1106 58-59 0.2772894487529527 1106 60-61 0.1709818731647701 1106 62-63 0.3053835567487191 1106 64-65 -0.04616606704823312 1106 66-67 0.14341019323289572 1106 68-69 0.1703685393270149 1106 70-71 -0.013713693532562132 1106 72-73 0.16219797019305204 1106 74-75 0.2575184949394753 1106 76-77 0.13069787985417136 1106 78-79 0.3282191513695416 1106 80-81 0.4403316388008278 1106 82-83 0.535970474401644 1106 84-85 0.5264020966051781 1106 86-87 0.22053221561416692 1106 88-89 0.4645447915607903 1106 90-91 0.5005895505069269 1106 92-93 0.5852480223383729 1106 94-95 0.6487965520503138 1106 96-97 0.7188096090599174 1106 98-99 0.5390298822442645 1106 100-101 0.7787862883050458 1107 1 0.10266481552713458 1107 2 0.1670143651403393 1107 3 0.16621690721255789 1107 4 0.16760854256123991 1107 5 0.06479128269396739 1107 6 0.0882195917823907 1107 7 0.07463961616628012 1107 8 0.18635802497897203 1107 9 0.23894890155981585 1107 10-11 0.1206901916358305 1107 12-13 0.17170467787867238 1107 14-15 0.22793820072509163 1107 16-17 0.16432342299115987 1107 18-19 0.16929705192224986 1107 20-21 0.164601646520822 1107 22-23 0.3017843518522696 1107 24-25 0.19615878096229977 1107 26-27 0.21687181231943242 1107 28-29 0.2574917061180031 1107 30-31 0.29453022680834806 1107 32-33 0.12986233838046246 1107 34-35 0.2229028813386762 1107 36-37 0.37456821307490173 1107 38-39 0.42421780048449165 1107 40-41 0.40812302512634346 1107 42-43 0.5029396595576969 1107 44-45 0.2069563172954858 1107 46-47 0.12810028246556016 1107 48-49 0.33110093735734836 1107 50-51 0.2646153313856203 1107 52-53 0.29022921194278695 1107 54-55 0.429048695572952 1107 56-57 0.49468714044481743 1107 58-59 0.5336405061635645 1107 60-61 0.6435634915554225 1107 62-63 0.4652186293678291 1107 64-65 0.4156637597285666 1107 66-67 0.681865583255366 1107 68-69 0.711005799528305 1107 70-71 0.4827672908640821 1107 72-73 0.2540327959309501 1107 74-75 0.336333020448361 1107 76-77 0.49354367647159236 1107 78-79 0.840545577396032 1107 80-81 0.8723007234465818 1107 82-83 0.7979836986472186 1107 84-85 0.743720813344261 1107 86-87 0.6836326755645921 1107 88-89 0.5342701400504843 1107 90-91 0.4273796884460488 1107 92-93 0.4260038928591463 1107 94-95 0.43057637388182 1107 96-97 0.4579575698748073 1107 98-99 0.18487495585243252 1107 100-101 0.19938618663905672 1108 1 0.07512476303552873 1108 2 0.02082698989519116 1108 3 0.010316839124776322 1108 4 0.09659822464368517 1108 5 0.20973561947824493 1108 6 0.15358153362262073 1108 7 0.07400529410525536 1108 8 -0.030638314575377024 1108 9 0.043059774072723656 1108 10-11 0.06902436879208551 1108 12-13 -0.03164791789523491 1108 14-15 0.021598471795932994 1108 16-17 0.19451016973371082 1108 18-19 0.148055992080657 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2316 58-59 -2.2843461082073695 2316 60-61 -1.9626271454308153 2316 62-63 -2.1977821412149545 2316 64-65 -2.110731731063403 2316 66-67 -2.2184729536763257 2316 68-69 -2.3689174885224666 2316 70-71 -2.1680825147585736 2316 72-73 -1.9077956498295414 2316 74-75 -2.122776739978317 2316 76-77 -1.6085135198192226 2316 78-79 -1.8485589015081807 2316 80-81 -1.850912866516797 2316 82-83 -1.6798468943551974 2316 84-85 -1.9176296979244292 2316 86-87 -2.045271105485998 2316 88-89 -2.3505467816011603 2316 90-91 -2.44959980019868 2316 92-93 -2.6823270594599613 2316 94-95 -2.655566123325901 2316 96-97 -2.105889975948891 2316 98-99 -2.149482646509533 2316 100-101 -1.9693939548364785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 19.0 4 44.0 5 95.0 6 260.0 7 612.0 8 1393.0 9 2605.0 10 4209.0 11 6519.0 12 9638.0 13 13343.0 14 17255.0 15 23232.0 16 29519.0 17 37599.0 18 33640.0 19 25352.0 20 24891.0 21 26528.0 22 29046.0 23 32160.0 24 37443.0 25 44039.0 26 52461.0 27 63177.0 28 75871.0 29 89790.0 30 106588.0 31 126960.0 32 152512.0 33 185818.0 34 228837.0 35 282554.0 36 334956.0 37 293584.0 38 90853.0 39 2000.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.307058547498535 22.27963343572911 16.472130788637973 20.941177228134382 2 16.11875115177335 23.24682995761474 39.01970709347531 21.6147117971366 3 19.617036411757276 26.93407032895229 31.232080392499256 22.21681286679118 4 13.593323258512498 19.50817653789887 37.8457586774625 29.052741526126134 5 12.733487915661298 39.10836333847937 34.54432211706171 13.613826628797618 6 29.232624985515454 39.19163372774209 17.64873211963589 13.927009167106569 7 26.222173582767898 34.571426502208084 21.724924036616926 17.48147587840709 8 25.39667531447553 36.15728282841288 20.2410630367328 18.204978820378788 9 26.174736703189172 16.806938740037854 21.103496890651353 35.91482766612162 10-11 24.003181289222887 28.287399261857203 27.786062524847534 19.923356924072372 12-13 25.286975015772057 25.734728463093383 28.523908348247044 20.454388172887512 14-15 22.30875172205127 26.594647639341307 27.462432727342957 23.63416791126447 16-17 20.742469646834643 29.975722295896688 27.976231669005653 21.305576388263013 18-19 20.661664118451757 28.112637804779915 31.21960247847429 20.00609559829404 20-21 21.683186900709405 27.049796882698125 31.433863505736433 19.83315271085604 22-23 21.31896946796778 27.160263092988252 31.2318109631735 20.288956475870467 24-25 21.08531508151291 27.41460957118027 31.32007475579525 20.18000059151157 26-27 20.9178400586976 27.528180420680886 31.244380664476303 20.30959885614521 28-29 20.648575942937132 27.535176444506497 31.441531118343573 20.374716494212798 30-31 21.04251329797935 27.261139320978216 31.420228218271944 20.276119162770488 32-33 20.907769320496794 27.41722230023647 31.24712057010398 20.427887809162755 34-35 21.159765318209324 27.46040086778509 31.10980169050715 20.270032123498435 36-37 21.289108553926088 27.232900557621566 31.002258170173967 20.475732718278383 38-39 20.984084885439785 27.635150510615208 31.06422080000708 20.316543803937925 40-41 21.24342929428775 27.36344731173667 31.002807028414498 20.390316365561084 42-43 21.05537601874622 27.54050843966061 30.681904138073108 20.722211403520067 44-45 21.262846985283705 27.595187590930742 30.40740192354736 20.73456350023819 46-47 21.43909719105407 27.881444363952095 29.806890122449175 20.872568322544655 48-49 21.431333338698124 28.117138902524403 29.508859954774803 20.94266780400267 50-51 21.42687429195561 28.161713063545996 29.343632916793833 21.06777972770456 52-53 21.5673442750647 28.38403191749604 29.088483661435067 20.960140146004196 54-55 21.498130836985084 28.238444122467094 29.13514344332378 21.12828159722404 56-57 21.597028641692365 28.48062710435001 28.866813555393893 21.055530698563732 58-59 21.744540277230413 28.176790467390532 29.021320665782707 21.057348589596344 60-61 21.72650876688981 28.263653679486467 29.16971449769733 20.84012305592639 62-63 21.602078620208204 28.16983043311629 29.314520063453042 20.913570883222466 64-65 21.52925054125944 28.19045331408498 29.138286003057047 21.14201014159854 66-67 21.634427941167594 28.44755196922786 28.77667867697501 21.141341412629533 68-69 21.534255746709636 28.34434530772807 28.891963287204618 21.229435658357676 70-71 21.5728620075791 28.256772135842855 28.98466697232 21.18569888425804 72-73 21.677587744145026 28.26952355508633 28.79102746913183 21.261861231636818 74-75 21.47772116288352 28.5828926276893 28.558168316831683 21.3812178925955 76-77 21.47685611376482 28.470154598359702 28.721843657057512 21.331145630817957 78-79 21.44938895452853 28.40233341373714 28.609405072843234 21.538872558891097 80-81 21.42369576601372 28.692111637814232 28.488259223501956 21.395933372670093 82-83 21.607989953206438 28.5654588758681 28.49290853627376 21.3336426346517 84-85 21.460244113048148 28.500182276011753 28.369128640188762 21.67044497075134 86-87 21.344934767082776 28.548057095148522 28.502248196915897 21.604759940852805 88-89 21.491176894237803 28.404655014110368 28.44404495684005 21.660123134811776 90-91 21.38269202759478 28.47674698378288 28.51193249531866 21.62862849330369 92-93 21.532269696944702 28.39161593213499 28.42390443889516 21.652209932025148 94-95 21.33781308252532 28.370745716898682 28.36195440022499 21.929486800351008 96-97 21.412855136540333 28.352843214747775 28.290779883594542 21.94352176511735 98-99 21.191165269760315 28.35819814571093 28.166114301555538 22.28452228297322 100-101 21.310348171250467 28.569540402039834 28.0197698519816 22.1003415747281 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1458.0 1 1005.5 2 594.5 3 693.5 4 652.0 5 622.0 6 1028.5 7 2283.5 8 3737.0 9 4436.0 10 4018.5 11 3375.5 12 3372.5 13 3602.0 14 3920.0 15 4205.0 16 4524.5 17 4885.5 18 5222.0 19 5572.5 20 6156.5 21 7141.5 22 8356.0 23 9933.5 24 11822.0 25 14444.0 26 18581.0 27 22932.0 28 26121.5 29 30481.0 30 36897.0 31 42290.0 32 47759.0 33 55133.5 34 63848.5 35 70748.5 36 77924.0 37 85366.5 38 87800.5 39 87616.5 40 87476.5 41 88204.5 42 87975.5 43 89105.0 44 91715.0 45 92202.5 46 90789.0 47 90053.0 48 92543.5 49 90847.5 50 84646.0 51 79028.0 52 73296.0 53 68681.0 54 63877.5 55 57542.0 56 51142.5 57 45154.5 58 39806.0 59 34784.5 60 29322.5 61 23430.5 62 17780.5 63 13159.0 64 9710.5 65 7200.0 66 5164.5 67 3779.5 68 2557.0 69 1551.5 70 1024.0 71 656.5 72 434.5 73 305.5 74 213.5 75 164.0 76 120.5 77 59.0 78 26.5 79 18.0 80 10.0 81 4.0 82 5.0 83 4.0 84 1.0 85 0.0 86 1.0 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024342079851678276 2 0.004425832700305141 3 8.046968546009348E-5 4 1.6093937092018695E-4 5 4.023484273004674E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010139180367971777 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.218787418403739E-4 20-21 8.650491186960047E-4 22-23 0.0020519769792323836 24-25 0.01003859326114666 26-27 0.005129942448080959 28-29 3.6211358457042067E-4 30-31 0.002735969305643178 32-33 0.004305128172115001 34-35 0.0 36-37 0.008449316973309815 38-39 0.012774562566789839 40-41 0.001589276287836846 42-43 0.0 44-45 0.0 46-47 3.82231005935444E-4 48-49 0.002735969305643178 50-51 0.0052104121335410526 52-53 0.0 54-55 0.002856673833833318 56-57 0.003218787418403739 58-59 1.0058710682511684E-4 60-61 0.001367984652821589 62-63 0.004244775908019931 64-65 4.023484273004674E-5 66-67 0.0017300982373920095 68-69 3.017613204753505E-4 70-71 8.650491186960047E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.001468571759646706 80-81 0.0012673975459964722 82-83 0.009535657727021076 84-85 0.0066789838931877584 86-87 0.0033394919465938792 88-89 8.046968546009348E-5 90-91 4.023484273004674E-4 92-93 1.4082194955516357E-4 94-95 4.023484273004674E-5 96-97 0.001750215658757033 98-99 0.0014082194955516358 100-101 3.017613204753505E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2485408.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.141713280463065 #Duplication Level Percentage of deduplicated Percentage of total 1 74.26160155788695 38.721271361793306 2 14.313351772457859 14.92645368403811 3 4.99228717860755 7.809192200420603 4 2.172144571835195 4.530373578733798 5 1.1515078533039949 3.002079616358921 6 0.694920793651557 2.1740616465126794 7 0.4598413319323196 1.6783840418884872 8 0.30016324606996075 1.252082073113038 9 0.23012286530450016 1.0799100415787284 >10 1.241587398718252 11.965531634294734 >50 0.1156186660295009 4.183061638731169 >100 0.06376942798423897 6.038029320345615 >500 0.002312502163641353 0.7753556059243639 >1k 5.395838381820817E-4 0.49746391242096905 >5k 7.708340545458311E-5 0.3270874460112989 >10k+ 1.5416681090916622E-4 1.0396621978341447 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14090 0.5669089340663586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11777 0.4738457428317604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8138 0.3274311501371203 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2942 0.1183709073117975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 3 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 4 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 5 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 6 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 7 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 8 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 9 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 10-11 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 12-13 4.023484273004674E-5 0.0 0.0 0.0 8.046968546009348E-5 14-15 4.023484273004674E-5 2.011742136502337E-5 0.0 0.0 1.6093937092018695E-4 16-17 4.023484273004674E-5 4.023484273004674E-5 0.0 6.035226409507011E-5 1.6093937092018695E-4 18-19 4.023484273004674E-5 4.023484273004674E-5 0.0 8.046968546009348E-5 1.6093937092018695E-4 20-21 4.023484273004674E-5 4.023484273004674E-5 0.0 1.0058710682511684E-4 1.6093937092018695E-4 22-23 4.023484273004674E-5 4.023484273004674E-5 0.0 2.615264777453038E-4 1.6093937092018695E-4 24-25 4.023484273004674E-5 4.023484273004674E-5 0.0 5.230529554906076E-4 1.6093937092018695E-4 26-27 4.023484273004674E-5 4.023484273004674E-5 0.0 8.449316973309815E-4 1.6093937092018695E-4 28-29 4.023484273004674E-5 4.023484273004674E-5 0.0 0.0016496285519319162 1.6093937092018695E-4 30-31 4.023484273004674E-5 4.023484273004674E-5 0.0 0.003077965468848575 1.6093937092018695E-4 32-33 4.023484273004674E-5 4.023484273004674E-5 0.0 0.005994991566776964 1.6093937092018695E-4 34-35 4.023484273004674E-5 8.046968546009348E-5 0.0 0.011466930178063321 1.6093937092018695E-4 36-37 4.023484273004674E-5 1.0058710682511684E-4 0.0 0.01947366388134262 1.6093937092018695E-4 38-39 4.023484273004674E-5 1.2070452819014021E-4 0.0 0.031242355379881293 1.6093937092018695E-4 40-41 8.046968546009348E-5 1.2070452819014021E-4 0.0 0.0444192663739716 1.6093937092018695E-4 42-43 8.046968546009348E-5 1.2070452819014021E-4 0.0 0.057435237997141717 1.8105679228521033E-4 44-45 8.046968546009348E-5 1.2070452819014021E-4 0.0 0.07475633779242682 2.011742136502337E-4 46-47 1.2070452819014021E-4 1.4082194955516357E-4 0.0 0.09543704695567086 2.011742136502337E-4 48-49 1.2070452819014021E-4 2.011742136502337E-4 0.0 0.11943713064414374 2.011742136502337E-4 50-51 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.14611283137416473 2.011742136502337E-4 52-53 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.17508191813979837 2.011742136502337E-4 54-55 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.210045996472209 2.011742136502337E-4 56-57 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.2537209182556747 2.011742136502337E-4 58-59 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.30341094902728244 2.4140905638028043E-4 60-61 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.36159053161493004 2.4140905638028043E-4 62-63 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.4199511709948628 2.8164389911032714E-4 64-65 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.48267729081100563 2.8164389911032714E-4 66-67 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.556186348478801 2.8164389911032714E-4 68-69 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.6466342749359462 2.8164389911032714E-4 70-71 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.7501384078589913 2.8164389911032714E-4 72-73 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.859094361971958 3.218787418403739E-4 74-75 1.2070452819014021E-4 2.4140905638028043E-4 0.0 0.9724157965211346 3.218787418403739E-4 76-77 1.2070452819014021E-4 2.8164389911032714E-4 0.0 1.0985520284798311 3.218787418403739E-4 78-79 1.2070452819014021E-4 2.8164389911032714E-4 0.0 1.2455701438154219 3.218787418403739E-4 80-81 1.2070452819014021E-4 2.8164389911032714E-4 0.0 1.4044776551777414 3.4199616320539723E-4 82-83 1.2070452819014021E-4 2.8164389911032714E-4 0.0 1.5731018810593673 3.621135845704206E-4 84-85 1.2070452819014021E-4 2.8164389911032714E-4 0.0 1.7483849734128158 3.621135845704206E-4 86-87 1.2070452819014021E-4 2.8164389911032714E-4 0.0 1.9381727265704463 3.621135845704206E-4 88-89 1.2070452819014021E-4 2.8164389911032714E-4 0.0 2.1471323822889445 3.621135845704206E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9220 0.0 40.0331 1 GTATCAA 14790 0.0 36.133785 1 ATCAACG 18990 0.0 28.087557 3 TCAACGC 19110 0.0 27.936037 4 TATCAAC 19145 0.0 27.83703 2 CAACGCA 19470 0.0 27.374016 5 AACGCAG 19965 0.0 26.695322 6 ACGCAGA 22785 0.0 23.224575 7 CGCAGAG 22745 0.0 23.098341 8 GAGTACT 13020 0.0 21.996664 12-13 GCAGAGT 23565 0.0 21.891424 9 AGTACTT 13615 0.0 21.087696 12-13 AGAGTAC 20250 0.0 20.842222 10-11 GTACTTT 13870 0.0 20.546318 14-15 GTACATG 12320 0.0 20.28169 1 TACATGG 12540 0.0 19.507208 2 ACATGGG 12505 0.0 18.649063 3 CAGAGTA 23110 0.0 18.59175 10-11 CGGGTCG 115 2.0622228E-6 18.585485 14-15 TACCGCG 130 0.007563727 18.269989 8 >>END_MODULE