FastQCFastQC Report
Thu 26 May 2016
SRR936804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471396
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160930.6511704316103125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113410.458890440868238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.3179579476538766No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.10160249510802802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77800.042.4642261
GTATCAA135950.040.3850631
ATCAACG170000.032.134263
TCAACGC170700.031.9752794
TATCAAC172400.031.7972222
CAACGCA172950.031.4755485
AACGCAG177200.030.7680596
ACGCAGA201700.026.8877057
CGCAGAG201650.026.7524078
GCAGAGT214700.024.4356049
GTACATG127850.024.353341
TACATGG127600.023.883862
GAGTACT113250.023.3101512-13
ACATGGG128200.022.7336753
CATTCCG4450.022.4256659
AGTACTT119100.022.08539212-13
GTACTTT122400.021.39202114-15
AGAGTAC188400.021.31276910-11
CATGGGA85100.019.7220084
CAGAGTA211700.019.57310310-11
Produced by FastQC (version 0.11.5)