##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936804_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2471396 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24017154676952 33.0 31.0 34.0 31.0 34.0 2 32.36139169926633 33.0 31.0 34.0 31.0 34.0 3 32.392175515376735 34.0 31.0 34.0 31.0 34.0 4 35.97458400029781 37.0 35.0 37.0 35.0 37.0 5 35.98657358027609 37.0 35.0 37.0 35.0 37.0 6 36.066227346811274 37.0 36.0 37.0 35.0 37.0 7 36.03274788823806 37.0 36.0 37.0 35.0 37.0 8 36.01210894571327 37.0 36.0 37.0 35.0 37.0 9 37.72418584476142 39.0 38.0 39.0 35.0 39.0 10-11 37.62276037510783 39.0 37.5 39.0 35.0 39.0 12-13 37.55459242468629 39.0 37.0 39.0 35.0 39.0 14-15 38.80736737455268 40.0 38.0 41.0 35.5 41.0 16-17 38.746727355713126 40.0 38.0 41.0 35.0 41.0 18-19 38.7310714268373 40.0 38.0 41.0 35.0 41.0 20-21 38.68349447033175 40.0 38.0 41.0 35.0 41.0 22-23 38.58330028858184 40.0 38.0 41.0 34.5 41.0 24-25 38.56107155631878 40.0 38.0 41.0 34.5 41.0 26-27 38.59288677330545 40.0 38.0 41.0 35.0 41.0 28-29 38.52682734778239 40.0 38.0 41.0 35.0 41.0 30-31 38.3656255411921 40.0 38.0 41.0 34.0 41.0 32-33 38.16753142758182 40.0 38.0 41.0 34.0 41.0 34-35 38.016666895956774 40.0 38.0 41.0 34.0 41.0 36-37 37.854815861157014 40.0 37.5 41.0 33.5 41.0 38-39 37.71489352576438 40.0 37.0 41.0 33.0 41.0 40-41 37.55132969382487 40.0 37.0 41.0 33.0 41.0 42-43 37.33708721710321 40.0 37.0 41.0 33.0 41.0 44-45 37.07146588405905 40.0 37.0 41.0 32.0 41.0 46-47 36.70731117149983 39.5 36.0 41.0 31.0 41.0 48-49 36.44073228248326 39.0 36.0 41.0 30.0 41.0 50-51 35.75995065137275 39.0 35.5 40.0 29.5 40.5 52-53 35.94990199870842 39.0 36.0 40.0 30.0 40.5 54-55 36.35190313490837 39.0 36.0 41.0 31.0 41.0 56-57 36.26012585599394 39.0 36.0 41.0 30.5 41.0 58-59 36.07826831475005 39.0 36.0 41.0 30.0 41.0 60-61 35.82373808163483 39.0 35.0 41.0 29.0 41.0 62-63 35.539445519860024 39.0 35.0 40.5 29.0 41.0 64-65 35.23920630283451 38.0 35.0 40.0 28.0 41.0 66-67 34.89085642284765 37.5 35.0 40.0 28.0 41.0 68-69 34.508726039857635 37.0 34.0 40.0 27.5 41.0 70-71 34.105765324537224 36.5 34.0 39.0 26.0 41.0 72-73 33.72138681943323 36.0 34.0 39.0 26.0 40.5 74-75 33.325159950084895 35.5 34.0 38.5 26.0 40.0 76-77 32.875335437946816 35.0 33.0 37.0 25.5 39.5 78-79 32.42429541845985 35.0 33.0 37.0 24.5 39.0 80-81 31.99566844002337 35.0 32.5 36.5 23.5 39.0 82-83 31.601410093728404 35.0 32.0 36.0 21.5 37.5 84-85 31.235569896528116 35.0 32.0 35.5 20.0 37.0 86-87 30.906871865132096 35.0 31.5 35.0 19.5 37.0 88-89 30.570615352618518 34.0 31.0 35.0 18.0 36.0 90-91 30.23897809173439 34.0 31.0 35.0 12.5 36.0 92-93 29.896193689720302 34.0 31.0 35.0 6.5 36.0 94-95 29.57491899315205 34.0 31.0 35.0 2.0 35.0 96-97 29.156922039203753 34.0 30.0 35.0 2.0 35.0 98-99 28.67178206163642 34.0 30.0 35.0 2.0 35.0 100-101 26.466000390062945 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25822592792810184 1101 2 0.18252277873470746 1101 3 0.18993731167307004 1101 4 0.182756220701485 1101 5 0.18700685808136797 1101 6 0.11430365007540644 1101 7 0.07553853705755387 1101 8 0.1198929221660876 1101 9 0.1826951418517737 1101 10-11 0.1981222704340766 1101 12-13 0.23709401314228984 1101 14-15 0.36732327472493154 1101 16-17 0.42011028519552696 1101 18-19 0.3526307405147975 1101 20-21 0.35691802697180464 1101 22-23 0.3659857909101305 1101 24-25 0.4071282371479086 1101 26-27 0.38220389451067405 1101 28-29 0.3540628866651616 1101 30-31 0.33444316360897375 1101 32-33 0.24091923418182404 1101 34-35 0.2878673319494993 1101 36-37 0.25748463951600087 1101 38-39 0.23061918606853737 1101 40-41 0.2596447533144186 1101 42-43 0.372520156717691 1101 44-45 0.389238354207734 1101 46-47 0.32806211284084696 1101 48-49 0.29221880291060387 1101 50-51 0.3239991617087554 1101 52-53 0.3611905612653956 1101 54-55 0.24190644061796007 1101 56-57 0.28037708585689103 1101 58-59 0.3509797879252332 1101 60-61 0.4073097053580952 1101 62-63 0.42603759252422435 1101 64-65 0.3010852759261198 1101 66-67 0.2999020537840593 1101 68-69 0.3314149164750262 1101 70-71 0.3473887581285524 1101 72-73 0.297668409705345 1101 74-75 0.42011948962093015 1101 76-77 0.4173830291676026 1101 78-79 0.4887979565015925 1101 80-81 0.453952639820411 1101 82-83 0.2901494629515682 1101 84-85 0.3554034459723816 1101 86-87 0.4738675700623105 1101 88-89 0.44031646340618025 1101 90-91 0.41141559383918036 1101 92-93 0.31605996761301114 1101 94-95 0.2917902605406475 1101 96-97 0.3512465424956659 1101 98-99 0.44647152805110935 1101 100-101 0.6050675669152454 1102 1 0.019841652828574752 1102 2 0.036480574060213655 1102 3 0.0449153795995656 1102 4 0.06358138874637831 1102 5 0.08452790448133385 1102 6 0.056290635335933814 1102 7 0.061149605179835476 1102 8 0.006699649555777398 1102 9 0.02114071488254865 1102 10-11 -0.0125594184987321 1102 12-13 -0.01831663638271408 1102 14-15 -0.04976620393966158 1102 16-17 0.05360878887340448 1102 18-19 -0.03614381361849439 1102 20-21 -0.09226971717109933 1102 22-23 0.037046641343181363 1102 24-25 -0.1442961822697839 1102 26-27 -0.046791250599945045 1102 28-29 -0.12925690907055554 1102 30-31 -0.13635000365280092 1102 32-33 -0.049070732069353085 1102 34-35 -0.013791033470084813 1102 36-37 -0.04983312150130814 1102 38-39 0.08645408486868433 1102 40-41 -0.05040437825937971 1102 42-43 0.005024258784580127 1102 44-45 0.05225338501985277 1102 46-47 0.04299614181635292 1102 48-49 0.16628144566718817 1102 50-51 0.12680703814331196 1102 52-53 0.17666118021135446 1102 54-55 0.05723004941129517 1102 56-57 0.26667740609779145 1102 58-59 0.06499038570824922 1102 60-61 0.03206299866518947 1102 62-63 0.030354843113890695 1102 64-65 0.14049337797435868 1102 66-67 -0.010021277187476585 1102 68-69 0.09699065088484815 1102 70-71 0.051704959894301794 1102 72-73 0.055231945127623305 1102 74-75 0.2272391394535873 1102 76-77 0.049833116802666666 1102 78-79 -0.07155154658894247 1102 80-81 0.027792232690270424 1102 82-83 0.19964574778418154 1102 84-85 0.11756281127076207 1102 86-87 0.04403278313671777 1102 88-89 0.07031410937916505 1102 90-91 0.056761787307571865 1102 92-93 0.2417154825622454 1102 94-95 0.1118922528399473 1102 96-97 -0.06502298893641978 1102 98-99 -0.10810670489035346 1102 100-101 0.00487534079813301 1103 1 0.11557466536818595 1103 2 0.1531742632732218 1103 3 0.12547710473464946 1103 4 0.13433000366983805 1103 5 0.14209107404388988 1103 6 0.20504261230280463 1103 7 0.20808422387746361 1103 8 0.24419504863047337 1103 9 0.12072630492902903 1103 10-11 0.11974162696794366 1103 12-13 0.08735211989134228 1103 14-15 0.2910922561602689 1103 16-17 0.40721876477802965 1103 18-19 0.39150304392783397 1103 20-21 0.2687352162697181 1103 22-23 0.3041307746468078 1103 24-25 0.33347494506937636 1103 26-27 0.30246300922377856 1103 28-29 0.3175269646385388 1103 30-31 0.3163547391134074 1103 32-33 0.33996772744544757 1103 34-35 0.3647520548386609 1103 36-37 0.20875784533999564 1103 38-39 0.35720808849762165 1103 40-41 0.24951437486355843 1103 42-43 0.25487274480695987 1103 44-45 0.2733886858165846 1103 46-47 0.1067140571921712 1103 48-49 0.11033910851623574 1103 50-51 0.23847177000259734 1103 52-53 0.19793685331675448 1103 54-55 0.26137560597842224 1103 56-57 0.2695944187613648 1103 58-59 0.2794867666605896 1103 60-61 0.3414931638752705 1103 62-63 0.2450059469832766 1103 64-65 0.2905544865883414 1103 66-67 0.33565799284413345 1103 68-69 0.4748325714749839 1103 70-71 0.4397526376802432 1103 72-73 0.3328895049205727 1103 74-75 0.3366172562444092 1103 76-77 0.3548984883914912 1103 78-79 0.43235474451656586 1103 80-81 0.27600491125040705 1103 82-83 0.32366460732826496 1103 84-85 0.316684035982437 1103 86-87 0.4145730656182103 1103 88-89 0.3078150590517801 1103 90-91 0.35119319619849776 1103 92-93 0.3366744179668153 1103 94-95 0.4742947227635135 1103 96-97 0.53142934640249 1103 98-99 0.4699439528728462 1103 100-101 0.7806005986898974 1104 1 0.02719583684459792 1104 2 -0.0028260952943881534 1104 3 0.062087226922571404 1104 4 0.09426273065733426 1104 5 0.09587120140470518 1104 6 0.049998511028711334 1104 7 -0.02264351818859467 1104 8 0.039453523428967685 1104 9 0.0055980326819522475 1104 10-11 0.07645128863214978 1104 12-13 -0.03794952626203241 1104 14-15 -0.1235296086546569 1104 16-17 0.017523066688262645 1104 18-19 0.09328257429206133 1104 20-21 0.08912461329624222 1104 22-23 0.00510699343588783 1104 24-25 5.877804265423947E-4 1104 26-27 0.033003785545751896 1104 28-29 0.04819775637290746 1104 30-31 0.1682541886243385 1104 32-33 -0.019497510095185078 1104 34-35 0.0295127721864219 1104 36-37 -0.06180913737598104 1104 38-39 0.07793359644721676 1104 40-41 0.08180933691939174 1104 42-43 0.11138314962100537 1104 44-45 0.06511577797885337 1104 46-47 -0.02025370383730518 1104 48-49 3.3214971708872554E-4 1104 50-51 -0.15331878770172125 1104 52-53 0.011029907848374876 1104 54-55 0.14613383738067398 1104 56-57 0.1364741158641536 1104 58-59 -0.05655334393163969 1104 60-61 -0.04387563746233525 1104 62-63 -0.1505511300715625 1104 64-65 -0.11775669233435337 1104 66-67 -0.15059352708281892 1104 68-69 -0.02675279637910677 1104 70-71 -0.16255557196998183 1104 72-73 -0.15208502222417053 1104 74-75 -0.1345646052251226 1104 76-77 -0.09904192007522994 1104 78-79 -0.06889140498256552 1104 80-81 -0.06586954711497484 1104 82-83 -0.03886035320503822 1104 84-85 0.015637767130204594 1104 86-87 0.11017954968493981 1104 88-89 0.047343071337337506 1104 90-91 -0.04132587523154285 1104 92-93 0.049720583777070004 1104 94-95 0.24755050044975135 1104 96-97 0.12278120172415896 1104 98-99 -0.020201502663962145 1104 100-101 0.20265136280624674 1105 1 0.13237356282414225 1105 2 0.13484819332126818 1105 3 0.2202176716508788 1105 4 0.18604900393057022 1105 5 0.14574138879041953 1105 6 0.12646772497833325 1105 7 0.08523995822394426 1105 8 0.0819795310679865 1105 9 0.14365251642379917 1105 10-11 0.10231882367521195 1105 12-13 0.13698522601120544 1105 14-15 0.2751314411716308 1105 16-17 0.23868969262844786 1105 18-19 0.07102030842666096 1105 20-21 0.18012196339230258 1105 22-23 0.11528285867854038 1105 24-25 0.02412939435607342 1105 26-27 0.15009961666594762 1105 28-29 0.08729639883975437 1105 30-31 0.14730419342860301 1105 32-33 0.05349251606242689 1105 34-35 -0.135899787080362 1105 36-37 -0.05059143018426937 1105 38-39 0.09430161850097107 1105 40-41 0.09450636651998678 1105 42-43 0.12448573694760512 1105 44-45 0.07833895959019088 1105 46-47 0.1653984539590354 1105 48-49 0.18293567424053236 1105 50-51 0.13353453306737606 1105 52-53 0.3220754907809038 1105 54-55 0.1838622897792206 1105 56-57 0.266800810536985 1105 58-59 0.33793836274208644 1105 60-61 0.42334588484968094 1105 62-63 0.30786213203188595 1105 64-65 0.32411503339059067 1105 66-67 0.1399750487535556 1105 68-69 0.17891496429355414 1105 70-71 0.05379703742695341 1105 72-73 0.18524731026195695 1105 74-75 0.23622428868750944 1105 76-77 0.07007150586085231 1105 78-79 0.1289037071567094 1105 80-81 0.09300494849851404 1105 82-83 0.11514037743293315 1105 84-85 0.0884183079681371 1105 86-87 0.048759380662719565 1105 88-89 0.20210492384114076 1105 90-91 0.19859700295530303 1105 92-93 0.08482132451130653 1105 94-95 0.2139025001688104 1105 96-97 0.08653792464214405 1105 98-99 0.13837059272087515 1105 100-101 0.07375253372919488 1106 1 0.2497787458393148 1106 2 0.21178005467145766 1106 3 0.2683294112369552 1106 4 0.21869979325317956 1106 5 0.10777109103000981 1106 6 0.10472552090342191 1106 7 0.18492432240382328 1106 8 0.1765265590961036 1106 9 0.1274228690301058 1106 10-11 0.2560335846022497 1106 12-13 0.27571816143987604 1106 14-15 0.30078782462059195 1106 16-17 0.36238389799603965 1106 18-19 0.42704283098068174 1106 20-21 0.35657116529124266 1106 22-23 0.1345402949201855 1106 24-25 0.1308299163243376 1106 26-27 0.053974409537225654 1106 28-29 0.11472517491389311 1106 30-31 0.2292937633854777 1106 32-33 0.20646441025800755 1106 34-35 0.3735941468544155 1106 36-37 0.1804059417183126 1106 38-39 0.09860092329423509 1106 40-41 0.2530378170697247 1106 42-43 0.28945148156613953 1106 44-45 0.1959018439873148 1106 46-47 0.2751555083768906 1106 48-49 0.4202863531721661 1106 50-51 0.43185330427063207 1106 52-53 0.47229285455759396 1106 54-55 0.5189571397803476 1106 56-57 0.48025118470875583 1106 58-59 0.5414514135025073 1106 60-61 0.6218387102136589 1106 62-63 0.685275720608395 1106 64-65 0.3684873857747135 1106 66-67 0.3028401798904099 1106 68-69 0.4174173294258523 1106 70-71 0.4670793392145711 1106 72-73 0.4581124569358934 1106 74-75 0.5850805263986558 1106 76-77 0.6121418453383285 1106 78-79 0.5809090783259379 1106 80-81 0.6404128582715707 1106 82-83 0.570547204271378 1106 84-85 0.6192161060528782 1106 86-87 0.8399208008452348 1106 88-89 0.8050237200591006 1106 90-91 0.8309983832696659 1106 92-93 0.8728395930122836 1106 94-95 1.0047549266337406 1106 96-97 1.0467847467262423 1106 98-99 1.1046327903012845 1106 100-101 1.4277880577134 1107 1 0.18948863283382167 1107 2 0.3099881563019764 1107 3 0.22009875785433763 1107 4 0.20197204638266442 1107 5 0.21400184120825116 1107 6 0.19053158234261502 1107 7 0.22646130124449115 1107 8 0.2662589436154761 1107 9 0.25060505343063255 1107 10-11 0.32468716433871947 1107 12-13 0.3995454466061332 1107 14-15 0.40537521838773927 1107 16-17 0.4495150697503689 1107 18-19 0.49107916485614567 1107 20-21 0.46913952041781926 1107 22-23 0.5483806718970854 1107 24-25 0.5707029595175541 1107 26-27 0.5838696140464421 1107 28-29 0.5678299350214004 1107 30-31 0.5951835028737662 1107 32-33 0.5461129120204617 1107 34-35 0.4941095713174306 1107 36-37 0.46713360860365327 1107 38-39 0.5265913554405799 1107 40-41 0.569721558537573 1107 42-43 0.57928630133582 1107 44-45 0.5737634661110462 1107 46-47 0.581060908691164 1107 48-49 0.6103930140799037 1107 50-51 0.5455459933681226 1107 52-53 0.5217137280038315 1107 54-55 0.6351282169515358 1107 56-57 0.7272170691339781 1107 58-59 0.7508593103709842 1107 60-61 0.8330120425600569 1107 62-63 0.8405718169116341 1107 64-65 0.7687928477995811 1107 66-67 0.8190677482855264 1107 68-69 0.7418216737324741 1107 70-71 0.7712075774109692 1107 72-73 0.7155713116810531 1107 74-75 0.811145945865988 1107 76-77 0.7236099976143677 1107 78-79 0.7574658440074771 1107 80-81 0.7195665934368627 1107 82-83 0.7893013009696546 1107 84-85 0.7887120787993531 1107 86-87 0.7954118982919844 1107 88-89 0.6787975575050993 1107 90-91 0.6821266554936471 1107 92-93 0.7849832675338995 1107 94-95 0.7552111471553076 1107 96-97 0.5981791526716087 1107 98-99 0.7804718585038302 1107 100-101 1.0007129135401236 1108 1 0.050090160581802934 1108 2 0.06828814936849881 1108 3 0.11085954970057088 1108 4 0.024829873301449368 1108 5 0.04229174823467474 1108 6 0.08593712565625111 1108 7 -0.011910095785815145 1108 8 0.004210134114885022 1108 9 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-2.731685642295858 2316 84-85 -2.5432274123741188 2316 86-87 -2.8212325359678907 2316 88-89 -2.4554973005947858 2316 90-91 -3.0275989835343964 2316 92-93 -3.0413815378735194 2316 94-95 -3.063943198036416 2316 96-97 -3.277022514475995 2316 98-99 -3.4517782620233284 2316 100-101 -3.8430298492212707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3427.0 3 979.0 4 918.0 5 924.0 6 1057.0 7 1189.0 8 1481.0 9 1843.0 10 2391.0 11 3452.0 12 5332.0 13 7299.0 14 8349.0 15 11471.0 16 16435.0 17 24749.0 18 21822.0 19 10315.0 20 8947.0 21 8898.0 22 9165.0 23 10293.0 24 11280.0 25 13022.0 26 15216.0 27 18170.0 28 22605.0 29 28657.0 30 36335.0 31 47902.0 32 65431.0 33 93285.0 34 140059.0 35 216672.0 36 348185.0 37 568095.0 38 575063.0 39 110631.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66418190163861 19.975130761838997 15.14602986737216 22.214657469150247 2 14.585464924266464 20.951865978513563 41.84719083297177 22.6154782642482 3 18.377360888346022 26.537625478632965 30.49134322390087 24.59367040912014 4 12.020664730056641 17.5569648333641 39.187611525096585 31.234758911482675 5 12.747251234104661 38.358876704550745 34.65565148860015 14.238220572744439 6 29.26730773982221 37.88675922411813 18.68141882901606 14.164514207043597 7 26.56944595692553 34.013416797999504 21.99914419434642 17.417993050728548 8 25.30963933385328 35.15237583853482 20.737756763958167 18.80022806365373 9 26.484761667228113 16.813789160685133 21.281718859319533 35.41973031276723 10-11 23.6637271440663 27.710883130139113 28.23555157599939 20.3898381497952 12-13 25.081046546042572 25.367720740749437 28.644827252531197 20.906405460676787 14-15 22.258342169326372 26.078831227877124 27.662609599714333 24.000217003082174 16-17 20.77809310051761 29.590312303098987 27.995653174492176 21.635941421891232 18-19 20.647297051927442 27.794559793107204 31.135394258830157 20.422748896135193 20-21 21.677070068464687 26.888385941410192 31.140026205358435 20.294517784766683 22-23 21.245465352112905 26.973237128440168 30.947314909667263 20.83398260977966 24-25 20.95185701203585 27.190645358269116 31.05566888326769 20.801828746427343 26-27 20.775188026818025 27.38616117763643 30.98915034578645 20.8495004497591 28-29 20.551135857821517 27.33822017917985 31.235321253125935 20.875322709872695 30-31 20.945045882770916 27.06779999821956 31.16313775190204 20.824016367107486 32-33 20.66076943013725 27.243739606813584 31.08763285929242 21.007858103756753 34-35 20.99888336743524 27.308798170333105 30.871420539119672 20.82089792311199 36-37 20.987356580208964 27.151759681348025 30.8040086829304 21.056875055512606 38-39 20.78866382246587 27.523580787704834 30.828360837592655 20.859394552236648 40-41 21.02716189662261 27.18340390473508 30.89930788230762 20.89012631633469 42-43 20.772616208151845 27.342316213877503 30.628380137743616 21.256687440227033 44-45 20.91865100560341 27.451431443193847 30.438116464186404 21.191801087016337 46-47 21.053555846576813 27.639326876330106 29.954658816241253 21.35245846085183 48-49 21.084178909771865 27.807659734942792 29.64207201531445 21.46608933997089 50-51 21.079731460090166 27.886335665985328 29.527797295785383 21.506135578139123 52-53 21.178873245420853 28.13410521293894 29.308090310156643 21.37893123148356 54-55 21.094027103525903 27.98580582476733 29.35407316701566 21.566093904691108 56-57 21.200302843548773 28.26957931696721 29.065433872751484 21.46468396673253 58-59 21.304638906294226 27.987809677034942 29.233386658746426 21.474164757924406 60-61 21.419222901403053 27.9739956894171 29.415064866119234 21.19171654306061 62-63 21.191895137345433 27.93842748507071 29.56259680139364 21.30708057619022 64-65 21.078213087341673 27.981261310838274 29.423302779949882 21.517222821870167 66-67 21.174208752314673 28.236912231398502 28.989910047801963 21.59896896848486 68-69 21.109220722865036 28.117561909203076 29.193565231752693 21.5796521361792 70-71 21.14495373920197 28.02440037471773 29.27376984008103 21.556876045999275 72-73 21.23448197724293 28.006409530129652 29.116180826440928 21.64292766618649 74-75 21.057407827821436 28.32635063851898 28.93004719743241 21.686194336227178 76-77 21.05336537245479 28.192477551536616 28.999134943720755 21.755022132287845 78-79 21.03982540403647 28.166388253423168 28.984670239664727 21.809116102875635 80-81 21.015330313066695 28.488293581741054 28.784266755262234 21.71210934993002 82-83 21.21387625969045 28.327131889304475 28.79509858262248 21.66389326838259 84-85 21.124348542868603 28.23286423429027 28.662697460455306 21.98008976238583 86-87 20.95044574404641 28.352652315191722 28.87004445804352 21.82685748271835 88-89 21.16669881421101 28.154448272239847 28.787771762285203 21.891081151263943 90-91 21.085689339688113 28.225324142933562 28.848483278428272 21.840503238950056 92-93 21.271756016881024 28.158574131358215 28.80719669892239 21.76247315283837 94-95 21.02835062028047 28.054425809850798 28.83911150966107 22.078112060207662 96-97 21.092639809345787 28.057758772969045 28.748410860798995 22.101190556886174 98-99 20.905231164898176 28.066063940170157 28.692529546894658 22.33617534803701 100-101 20.988339912386365 28.041443782602553 28.788455472653236 22.181760832357845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1202.0 1 894.0 2 694.0 3 825.5 4 708.0 5 663.0 6 1188.0 7 2644.0 8 4687.5 9 5429.0 10 4396.5 11 3495.5 12 3465.0 13 3682.5 14 3838.5 15 4001.0 16 4181.0 17 4357.0 18 4627.5 19 4965.5 20 5479.0 21 6351.5 22 7518.0 23 9034.5 24 10872.0 25 13461.5 26 17620.0 27 22112.0 28 25072.0 29 28949.0 30 35044.5 31 40779.5 32 46729.5 33 53907.5 34 62890.5 35 69887.5 36 77170.5 37 84702.0 38 86445.5 39 86487.5 40 86364.0 41 86551.5 42 86355.0 43 88544.5 44 92162.0 45 92398.0 46 90365.0 47 89508.0 48 93386.5 49 91887.0 50 84878.5 51 79342.0 52 73447.0 53 69500.0 54 64923.5 55 58562.5 56 52617.0 57 46304.5 58 40773.0 59 35891.0 60 30366.5 61 24064.5 62 17983.5 63 13057.0 64 9524.5 65 7122.0 66 5023.0 67 3629.0 68 2476.5 69 1451.0 70 941.5 71 631.5 72 435.5 73 309.0 74 220.0 75 171.0 76 118.0 77 58.0 78 35.5 79 25.0 80 22.0 81 20.5 82 17.5 83 14.5 84 12.0 85 8.0 86 8.0 87 8.5 88 5.0 89 2.5 90 2.5 91 1.5 92 1.5 93 2.0 94 2.5 95 2.0 96 1.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035364627926888284 2 0.025410739517260687 3 0.0225378692852137 4 0.04450925711622095 5 0.08889712534939767 6 0.101764346952087 7 0.048919719866828304 8 0.07728425553816548 9 0.06360777471518121 10-11 0.06273782105336417 12-13 0.06146323778139966 14-15 0.05604120100542365 16-17 0.04400347010353663 18-19 0.0535931918640315 20-21 0.052217451189530126 22-23 0.028202683827278186 24-25 0.007890277397875532 26-27 0.0029537961540764815 28-29 0.0055029626980055 30-31 0.0039653701794451395 32-33 0.0050781016073506635 34-35 0.00511856456836541 36-37 0.0044711571921294685 38-39 0.0020838424922594357 40-41 0.005664814542064485 42-43 0.002650323946465884 44-45 0.0017803702846488382 46-47 9.104166228317923E-4 48-49 2.023148050737316E-4 50-51 0.001274583271964509 52-53 0.004552083114158961 54-55 0.009771805085061237 56-57 0.006190833035256187 58-59 0.0037023609328492883 60-61 0.0014768980770382407 62-63 0.006615694125911024 64-65 0.0015578239990677333 66-67 0.019118749079467638 68-69 0.04062481285880531 70-71 0.051387960488727825 72-73 0.0030751850371207204 74-75 0.011612869811232196 76-77 0.018552267625261187 78-79 0.022193934116588356 80-81 0.07066856141225444 82-83 0.024298008089355165 84-85 0.008294907008022996 86-87 0.025876063568930272 88-89 0.04109013691047489 90-91 0.037205692653059244 92-93 0.03763055374371408 94-95 0.008719768098677833 96-97 0.013170693810299927 98-99 0.03194550772114222 100-101 0.06834194115390654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2471396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.29811704894879 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3833524300486 37.64427802400894 2 14.751173592554794 15.134148591204767 3 5.130090755019203 7.8949198806811545 4 2.29900569927819 4.717386538310918 5 1.2651370887230988 3.2449575230141923 6 0.7108698840627844 2.1879771911535224 7 0.47550557007457933 1.7074778273779023 8 0.32633745085744376 1.3392397401232579 9 0.2185227224216866 1.0088823773378364 >10 1.2500027252962314 11.926370619610156 >50 0.11900787378311113 4.235318773998447 >100 0.06784323195374901 6.2948259215859075 >500 0.0023632319449812655 0.8087209866948492 >1k 5.514207871615279E-4 0.42935394411750366 >5k 7.877439816593256E-5 0.31754007462356176 >10k+ 1.5754879633186512E-4 1.1086019861571383 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16093 0.6511704316103125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11341 0.458890440868238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7858 0.3179579476538766 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2511 0.10160249510802802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0462961014746324E-5 2 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 3 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 4 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 5 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 6 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 7 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 8 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 9 0.0 4.0462961014746324E-5 0.0 0.0 4.0462961014746324E-5 10-11 0.0 4.0462961014746324E-5 0.0 2.0231480507373162E-5 4.0462961014746324E-5 12-13 4.0462961014746324E-5 4.0462961014746324E-5 0.0 4.0462961014746324E-5 4.0462961014746324E-5 14-15 4.0462961014746324E-5 8.092592202949265E-5 0.0 4.0462961014746324E-5 4.0462961014746324E-5 16-17 4.0462961014746324E-5 1.2138888304423896E-4 0.0 1.0115740253686581E-4 4.0462961014746324E-5 18-19 4.0462961014746324E-5 1.2138888304423896E-4 0.0 1.4162036355161212E-4 8.092592202949265E-5 20-21 4.0462961014746324E-5 1.2138888304423896E-4 0.0 2.630092465958511E-4 8.092592202949265E-5 22-23 4.0462961014746324E-5 1.2138888304423896E-4 0.0 5.462499736990753E-4 8.092592202949265E-5 24-25 4.0462961014746324E-5 1.8208332456635845E-4 0.0 7.283332982654338E-4 8.092592202949265E-5 26-27 4.0462961014746324E-5 2.023148050737316E-4 0.0 0.0012138888304423895 8.092592202949265E-5 28-29 4.0462961014746324E-5 2.2254628558110478E-4 0.0 0.0018815276871857038 8.092592202949265E-5 30-31 4.0462961014746324E-5 2.4277776608847793E-4 0.0 0.003884444257415647 1.0115740253686581E-4 32-33 4.0462961014746324E-5 2.4277776608847793E-4 0.0 0.007546342229250189 1.2138888304423896E-4 34-35 4.0462961014746324E-5 2.4277776608847793E-4 0.0 0.013919258589072735 1.2138888304423896E-4 36-37 4.0462961014746324E-5 2.8324072710322423E-4 0.0 0.023974304401237195 1.2138888304423896E-4 38-39 4.0462961014746324E-5 3.237036881179706E-4 0.0 0.03783286854878781 1.2138888304423896E-4 40-41 4.0462961014746324E-5 3.4393516862534374E-4 0.0 0.05290532152678082 1.618518440589853E-4 42-43 4.0462961014746324E-5 3.641666491327169E-4 0.0 0.0701830058800775 1.618518440589853E-4 44-45 4.0462961014746324E-5 3.641666491327169E-4 0.0 0.09083934747810549 2.023148050737316E-4 46-47 4.0462961014746324E-5 3.641666491327169E-4 0.0 0.11566337406065236 2.4277776608847793E-4 48-49 4.0462961014746324E-5 4.0462961014746325E-4 0.0 0.14218684500581857 2.4277776608847793E-4 50-51 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.1751034637913147 2.4277776608847793E-4 52-53 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.21002299914704078 2.630092465958511E-4 54-55 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.2494136916948963 2.8324072710322423E-4 56-57 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.29833341156172466 2.8324072710322423E-4 58-59 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.3555480384365759 2.8324072710322423E-4 60-61 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.41972229460596355 2.8324072710322423E-4 62-63 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.48444280074905033 2.8324072710322423E-4 64-65 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.5563859454332694 2.8324072710322423E-4 66-67 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.6425113579531568 2.8324072710322423E-4 68-69 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.7414028346731969 2.8324072710322423E-4 70-71 4.0462961014746324E-5 4.8555553217695586E-4 0.0 0.8544765792289055 3.2370368811797054E-4 72-73 4.0462961014746324E-5 4.8555553217695586E-4 0.0 0.9723654161453689 3.641666491327169E-4 74-75 4.0462961014746324E-5 4.8555553217695586E-4 0.0 1.0953728176301976 3.641666491327169E-4 76-77 4.0462961014746324E-5 4.8555553217695586E-4 0.0 1.2321578573405476 3.641666491327169E-4 78-79 4.0462961014746324E-5 4.8555553217695586E-4 0.0 1.384804377768678 3.641666491327169E-4 80-81 4.0462961014746324E-5 4.8555553217695586E-4 0.0 1.559260434183757 3.641666491327169E-4 82-83 8.092592202949265E-5 4.8555553217695586E-4 0.0 1.740574962490835 3.641666491327169E-4 84-85 8.092592202949265E-5 4.8555553217695586E-4 0.0 1.92961791635173 3.641666491327169E-4 86-87 8.092592202949265E-5 4.8555553217695586E-4 0.0 2.1336523972685884 3.641666491327169E-4 88-89 8.092592202949265E-5 4.8555553217695586E-4 0.0 2.3614386363011026 3.641666491327169E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7780 0.0 42.464226 1 GTATCAA 13595 0.0 40.385063 1 ATCAACG 17000 0.0 32.13426 3 TCAACGC 17070 0.0 31.975279 4 TATCAAC 17240 0.0 31.797222 2 CAACGCA 17295 0.0 31.475548 5 AACGCAG 17720 0.0 30.768059 6 ACGCAGA 20170 0.0 26.887705 7 CGCAGAG 20165 0.0 26.752407 8 GCAGAGT 21470 0.0 24.435604 9 GTACATG 12785 0.0 24.35334 1 TACATGG 12760 0.0 23.88386 2 GAGTACT 11325 0.0 23.31015 12-13 ACATGGG 12820 0.0 22.733675 3 CATTCCG 445 0.0 22.425665 9 AGTACTT 11910 0.0 22.085392 12-13 GTACTTT 12240 0.0 21.392021 14-15 AGAGTAC 18840 0.0 21.312769 10-11 CATGGGA 8510 0.0 19.722008 4 CAGAGTA 21170 0.0 19.573103 10-11 >>END_MODULE