FastQCFastQC Report
Thu 26 May 2016
SRR936804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471396
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139210.5632848802862835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116540.4715553476658536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83730.33879637257647094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.12106517935612099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91450.042.2860831
GTATCAA146900.035.8969881
TATCAAC192350.027.1147382
ATCAACG192200.027.1106383
TCAACGC193850.026.9288854
CAACGCA197550.026.3764345
AACGCAG202350.025.776316
ACGCAGA230650.022.4694757
CGCAGAG231250.022.3299128
GAGTACT133400.022.16646212-13
GTACATG127450.021.3585411
AGTACTT140350.021.28879412-13
GCAGAGT241550.021.0040689
GTACTTT142850.020.74869714-15
AGAGTAC209900.020.69714710-11
TACATGG130950.020.2406272
ACATGGG129800.019.43153
TCGTGCG650.00756640318.269382-83
CAGAGTA238500.018.23514410-11
ACTTTTT167900.017.93579716-17
Produced by FastQC (version 0.11.5)