##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936804_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2471396 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.407072763733535 31.0 31.0 34.0 30.0 34.0 2 31.56296724604232 31.0 31.0 34.0 30.0 34.0 3 31.65933423862465 31.0 31.0 34.0 30.0 34.0 4 35.4703329616136 37.0 35.0 37.0 33.0 37.0 5 35.243476156795595 37.0 35.0 37.0 33.0 37.0 6 35.36912012482014 37.0 35.0 37.0 33.0 37.0 7 35.30796035924635 37.0 35.0 37.0 32.0 37.0 8 35.2543262997917 37.0 35.0 37.0 32.0 37.0 9 36.804856850136524 39.0 37.0 39.0 33.0 39.0 10-11 36.6600304038689 38.0 35.0 39.0 32.0 39.0 12-13 36.51873273243139 38.0 35.0 39.0 32.0 39.0 14-15 37.43963533161015 39.0 36.0 40.0 32.0 41.0 16-17 37.42398607912289 39.0 36.0 40.0 32.0 41.0 18-19 37.59412210750523 39.0 36.5 40.5 32.0 41.0 20-21 37.606006888414484 39.0 36.5 41.0 32.0 41.0 22-23 37.47721834137467 39.0 36.0 40.5 32.0 41.0 24-25 37.35207631638151 39.0 36.0 40.5 32.0 41.0 26-27 37.06577072229622 39.0 36.0 40.0 31.0 41.0 28-29 36.851448938170975 39.0 36.0 40.0 31.0 41.0 30-31 36.75970301805134 39.0 35.5 40.0 30.0 41.0 32-33 36.57270829927701 38.0 35.0 40.0 30.0 41.0 34-35 36.38182549457878 38.0 35.0 40.0 30.0 41.0 36-37 36.16526307398733 38.0 35.0 40.0 30.0 41.0 38-39 35.940596933878666 38.0 35.0 40.0 30.0 41.0 40-41 35.541385921155495 38.0 34.5 40.0 28.0 41.0 42-43 35.248339602394765 38.0 34.0 40.0 27.0 41.0 44-45 34.92696759240526 38.0 34.0 40.0 26.0 41.0 46-47 34.448240994158766 38.0 33.0 40.0 24.5 41.0 48-49 33.992286748056564 38.0 33.0 40.0 23.5 41.0 50-51 33.64476494256687 37.0 33.0 40.0 22.5 41.0 52-53 33.31223446181834 37.0 32.0 40.0 21.5 40.5 54-55 32.84009563825465 37.0 31.0 39.0 19.0 40.0 56-57 32.567597018041624 36.0 31.0 39.0 18.0 40.0 58-59 32.1769926794411 36.0 31.0 39.0 16.0 40.0 60-61 31.709084663081107 35.5 30.0 39.0 13.5 40.0 62-63 31.24689143302004 35.0 29.0 38.0 10.5 40.0 64-65 30.844634166276876 35.0 29.0 38.0 8.0 40.0 66-67 30.387287185056543 34.0 28.0 38.0 7.5 40.0 68-69 29.82786894532483 34.0 28.0 37.0 7.0 39.0 70-71 29.299444322156383 34.0 27.0 36.5 3.5 39.0 72-73 28.77868014676725 33.0 26.0 36.0 2.0 39.0 74-75 28.1965316363707 32.5 26.0 36.0 2.0 38.0 76-77 27.074805089916794 31.5 25.5 34.0 2.0 36.5 78-79 27.73517093173251 32.0 26.0 35.0 2.0 37.0 80-81 27.517285979260304 32.0 26.0 35.0 2.0 37.0 82-83 27.030610230007657 32.0 25.5 35.0 2.0 36.0 84-85 26.403734771764622 32.0 24.5 34.0 2.0 36.0 86-87 25.830971442860633 31.0 23.0 34.0 2.0 35.0 88-89 25.233442960982376 31.0 20.5 34.0 2.0 35.0 90-91 24.489389802362712 31.0 18.5 34.0 2.0 35.0 92-93 23.6774952294169 30.0 10.5 34.0 2.0 35.0 94-95 22.742348251757306 29.5 2.0 34.0 2.0 35.0 96-97 21.52356380766174 29.0 2.0 34.0 2.0 35.0 98-99 20.13386138846223 28.0 2.0 33.0 2.0 34.0 100-101 16.822933475655056 21.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2119548979135608 1101 2 0.3812121253731071 1101 3 0.28213664444586684 1101 4 0.224522266966531 1101 5 0.0813269652155384 1101 6 0.20986221775216762 1101 7 0.19025367510192126 1101 8 0.2826083619059574 1101 9 0.29733943010682395 1101 10-11 0.2904136043976919 1101 12-13 0.2794896738101613 1101 14-15 0.46575525231987314 1101 16-17 0.4897222180597396 1101 18-19 0.33648057779185336 1101 20-21 0.13412550779703025 1101 22-23 0.32772555321045616 1101 24-25 0.295148665617738 1101 26-27 0.3836367997932868 1101 28-29 0.3887484019942704 1101 30-31 0.549065812200837 1101 32-33 0.5849409374813987 1101 34-35 0.34510559268698415 1101 36-37 0.572018003496872 1101 38-39 0.5319754602328217 1101 40-41 0.738766067893927 1101 42-43 0.7004011751734893 1101 44-45 0.5182380788302794 1101 46-47 0.4698718672515625 1101 48-49 0.5551195112012266 1101 50-51 0.584880883171266 1101 52-53 0.693562391709321 1101 54-55 0.5179091837446634 1101 56-57 0.7271527835348408 1101 58-59 0.7725774454417547 1101 60-61 0.7258797066532736 1101 62-63 0.6223270867641304 1101 64-65 0.759139278670343 1101 66-67 0.7856589617525884 1101 68-69 0.6026752857607356 1101 70-71 0.4637549097203184 1101 72-73 0.622792695119724 1101 74-75 0.4828591907812978 1101 76-77 0.5762412815420248 1101 78-79 0.8460892470883046 1101 80-81 0.793410682980678 1101 82-83 1.0599979754200568 1101 84-85 0.7562414599910205 1101 86-87 0.43146133303395473 1101 88-89 0.717530645553758 1101 90-91 0.8844900886775768 1101 92-93 0.7881144627596548 1101 94-95 0.7139026653283196 1101 96-97 0.6135363022213447 1101 98-99 0.946337810226499 1101 100-101 1.277127466404412 1102 1 -0.06458815514294258 1102 2 0.04927508743831055 1102 3 0.1005394254607559 1102 4 0.054570373023608454 1102 5 -0.14844949544657737 1102 6 0.13081037788194294 1102 7 0.1192111208194575 1102 8 0.20669355437679826 1102 9 0.28638947532279246 1102 10-11 0.36482189290351386 1102 12-13 0.39413032342838505 1102 14-15 0.3000369361251032 1102 16-17 0.25067957287919995 1102 18-19 0.33251340131906915 1102 20-21 0.23536725704887118 1102 22-23 0.05590150857251075 1102 24-25 0.06634329936842676 1102 26-27 0.26009909340803716 1102 28-29 0.24630319721723026 1102 30-31 0.20701688806111207 1102 32-33 0.13264593594312402 1102 34-35 0.3261089442620815 1102 36-37 0.22134815462074897 1102 38-39 0.45559590717164866 1102 40-41 0.4922213017583488 1102 42-43 0.5413788422161758 1102 44-45 0.2929311812980444 1102 46-47 0.36355765592053046 1102 48-49 0.48669318991616706 1102 50-51 0.4868493508628262 1102 52-53 0.5487217896377743 1102 54-55 0.667016051909826 1102 56-57 0.7400154435387591 1102 58-59 0.6216315810710142 1102 60-61 0.7528037158642746 1102 62-63 0.7266887375663629 1102 64-65 0.7707239467292268 1102 66-67 0.7352574952636743 1102 68-69 0.605207962452603 1102 70-71 0.4769847163638019 1102 72-73 0.55953859072212 1102 74-75 0.6480171436634663 1102 76-77 0.8505837854809428 1102 78-79 0.490334466781956 1102 80-81 0.8129891373405229 1102 82-83 1.0083492824877318 1102 84-85 0.7807044038766087 1102 86-87 0.6921119068571748 1102 88-89 0.7413181468356527 1102 90-91 0.8150061450064143 1102 92-93 0.6185470793189545 1102 94-95 0.546242976059883 1102 96-97 1.0464015493242584 1102 98-99 0.8059224293402671 1102 100-101 0.7585027425947111 1103 1 0.13407676416466074 1103 2 0.2479134101308773 1103 3 0.2715540412258193 1103 4 0.22445567338670003 1103 5 0.020863052593561804 1103 6 -0.008165955409261016 1103 7 0.008699756953127746 1103 8 0.15378421425847222 1103 9 0.18395434706870617 1103 10-11 0.17628698825757994 1103 12-13 0.2674520914742189 1103 14-15 0.24598584957821146 1103 16-17 0.2814636514978659 1103 18-19 0.24486315790800717 1103 20-21 0.3032643101092347 1103 22-23 0.29851033816302674 1103 24-25 0.3399163129163938 1103 26-27 0.25440516285966197 1103 28-29 0.26086426576284083 1103 30-31 0.47791561196708443 1103 32-33 0.47645280427612136 1103 34-35 0.59194500249572 1103 36-37 0.49552780226647997 1103 38-39 0.36037331836583775 1103 40-41 0.6488184706990126 1103 42-43 0.406031617046807 1103 44-45 0.522267953072614 1103 46-47 0.5757352167727205 1103 48-49 0.49507962300596375 1103 50-51 0.3542403050302738 1103 52-53 0.4005607200745587 1103 54-55 0.583823690272645 1103 56-57 0.30050481894269865 1103 58-59 0.2901338574521617 1103 60-61 0.121854645722415 1103 62-63 0.5067402818063051 1103 64-65 0.4621562056710964 1103 66-67 0.5458553675113436 1103 68-69 0.5873679360040036 1103 70-71 0.349832717749603 1103 72-73 0.5851784214427589 1103 74-75 0.42329633006607637 1103 76-77 0.615464447047458 1103 78-79 0.6444282582416001 1103 80-81 0.5803100383367052 1103 82-83 0.8725300830389067 1103 84-85 0.623123102360843 1103 86-87 0.4547193673001999 1103 88-89 0.7479457342092921 1103 90-91 0.5842723571176585 1103 92-93 0.46207699594318896 1103 94-95 0.8116370679340719 1103 96-97 0.9280004730633209 1103 98-99 0.8526730341530744 1103 100-101 1.3409280231629772 1104 1 -0.04594808604903733 1104 2 0.09934570519689956 1104 3 0.057073547234249844 1104 4 0.06922489516330188 1104 5 -0.19639599378757566 1104 6 -0.007500225669886618 1104 7 0.06539779751516761 1104 8 0.055679123915382434 1104 9 0.00998448384422801 1104 10-11 0.1436333084228778 1104 12-13 0.22802097561506685 1104 14-15 0.24266598024284036 1104 16-17 0.1389318730972704 1104 18-19 0.1848324832810775 1104 20-21 0.19063255326427964 1104 22-23 0.12208987790730674 1104 24-25 0.11387293778791019 1104 26-27 0.15110425001400785 1104 28-29 0.041929805313507984 1104 30-31 0.2558943973331793 1104 32-33 0.18093868833356197 1104 34-35 0.4000725772456306 1104 36-37 0.25171979350190554 1104 38-39 0.3158886980743816 1104 40-41 0.26448586505152605 1104 42-43 0.35751253360290036 1104 44-45 0.5133655072580154 1104 46-47 0.2084728088202823 1104 48-49 0.3672991356913258 1104 50-51 0.4463714442009348 1104 52-53 0.478708935565372 1104 54-55 0.5642770195335842 1104 56-57 0.44228284295332543 1104 58-59 0.043589864649391075 1104 60-61 0.3842755939257998 1104 62-63 0.5685206485202379 1104 64-65 0.5305822024694002 1104 66-67 0.35727941921901163 1104 68-69 0.4925920719473318 1104 70-71 0.5309894124863526 1104 72-73 0.5850195843107748 1104 74-75 0.4710413857260711 1104 76-77 0.6035514311126846 1104 78-79 0.7616148864423415 1104 80-81 0.7716250318919613 1104 82-83 0.8137895124774452 1104 84-85 0.8408283944444257 1104 86-87 0.5871640636763793 1104 88-89 0.74443508703866 1104 90-91 0.835032002921249 1104 92-93 0.6583995178009125 1104 94-95 0.6622303563894647 1104 96-97 0.7213524402063314 1104 98-99 0.8021990988066037 1104 100-101 1.331634260414134 1105 1 0.048666848717868305 1105 2 0.2213368838895846 1105 3 0.1780426619283162 1105 4 0.12563643846286965 1105 5 0.01093643136958633 1105 6 0.12320812735603681 1105 7 0.16923910958098531 1105 8 0.12433958357348018 1105 9 0.2007904926343187 1105 10-11 0.16305459730142502 1105 12-13 0.1765201437705599 1105 14-15 0.20924990142758304 1105 16-17 -0.03626272388857643 1105 18-19 0.1384359965864448 1105 20-21 0.15095678971589876 1105 22-23 0.10458736725379225 1105 24-25 0.08881749368146785 1105 26-27 0.10565483693870448 1105 28-29 0.1641123009250478 1105 30-31 0.251661281874334 1105 32-33 0.1946149825455734 1105 34-35 0.1345661519723791 1105 36-37 0.30059913239647784 1105 38-39 0.2167911676599914 1105 40-41 0.2798293843951427 1105 42-43 0.4759556309031012 1105 44-45 0.3820288202086033 1105 46-47 0.3741856954109295 1105 48-49 0.4861214526331068 1105 50-51 0.6227991756675806 1105 52-53 0.6529259750378671 1105 54-55 0.7682018330818963 1105 56-57 0.578887878502421 1105 58-59 0.5448829914336102 1105 60-61 0.5306060890866604 1105 62-63 0.7908874863356736 1105 64-65 0.7083941465955945 1105 66-67 0.7546775822839464 1105 68-69 0.6767727112116617 1105 70-71 0.4738968589944328 1105 72-73 0.5620100972352127 1105 74-75 0.6694970228809147 1105 76-77 0.5923054348673276 1105 78-79 0.8857494486911826 1105 80-81 0.7705640704445749 1105 82-83 0.7995982843276899 1105 84-85 0.6110267787492667 1105 86-87 0.6971580010395364 1105 88-89 0.990514736332436 1105 90-91 1.053458858402717 1105 92-93 0.8041356989640107 1105 94-95 0.9032301695841625 1105 96-97 1.075400555009164 1105 98-99 1.0428618820690438 1105 100-101 1.263088786004264 1106 1 0.15025295173027686 1106 2 0.2526390544976209 1106 3 0.239691084176485 1106 4 0.23952557982934053 1106 5 0.21956642586169295 1106 6 0.18870081210942402 1106 7 0.19551309203806255 1106 8 0.3185954466855776 1106 9 0.4799654425078117 1106 10-11 0.45259103292391245 1106 12-13 0.4484891892421672 1106 14-15 0.5459724549780773 1106 16-17 0.4913117123289794 1106 18-19 0.4085134023288006 1106 20-21 0.44992248616947705 1106 22-23 0.3786780012677369 1106 24-25 0.47510683454407143 1106 26-27 0.6980554170933502 1106 28-29 0.5597198124826974 1106 30-31 0.6472095583069901 1106 32-33 0.7261739034330859 1106 34-35 0.577036027512591 1106 36-37 0.4682087057248978 1106 38-39 0.6290235511338196 1106 40-41 0.6304634527101314 1106 42-43 0.7594889264813247 1106 44-45 0.5845803198089072 1106 46-47 0.5286794467849845 1106 48-49 0.4712567378900445 1106 50-51 0.6383925795548038 1106 52-53 0.44626200405492256 1106 54-55 0.7292406579191706 1106 56-57 0.7066359027682338 1106 58-59 0.7628307681937088 1106 60-61 0.7446062817464778 1106 62-63 0.7351202380383448 1106 64-65 0.8009060110998085 1106 66-67 0.8408355571185311 1106 68-69 0.41599929857706286 1106 70-71 0.5572639854810397 1106 72-73 0.5561223200245387 1106 74-75 0.6093399662878696 1106 76-77 0.7897093049990715 1106 78-79 0.8397723099535845 1106 80-81 0.8187719457253131 1106 82-83 0.9844033289074652 1106 84-85 0.8927119384277411 1106 86-87 0.8597219069997983 1106 88-89 1.219848475183607 1106 90-91 1.021848859358247 1106 92-93 1.2688465189102054 1106 94-95 1.4038679667988347 1106 96-97 1.3660207020987194 1106 98-99 1.4720582342729394 1106 100-101 1.5455142876383796 1107 1 0.32015976357952525 1107 2 0.37902850881842554 1107 3 0.3223000433022989 1107 4 0.2539671063965514 1107 5 0.1488393321582464 1107 6 0.21299813896423103 1107 7 0.2999265519431944 1107 8 0.24260505737875349 1107 9 0.43958906210266235 1107 10-11 0.45786307266922677 1107 12-13 0.4774779177042916 1107 14-15 0.5715081963532 1107 16-17 0.5333601895807263 1107 18-19 0.43489759660763383 1107 20-21 0.45291969333121784 1107 22-23 0.405200068296125 1107 24-25 0.43454113329845967 1107 26-27 0.6508125806129996 1107 28-29 0.5431188895622441 1107 30-31 0.4862553243324612 1107 32-33 0.40279370827739314 1107 34-35 0.5705831219020112 1107 36-37 0.5710827221614423 1107 38-39 0.6052888996448118 1107 40-41 0.8173599289836204 1107 42-43 0.6468506860033258 1107 44-45 0.6465704566879467 1107 46-47 0.523276719399 1107 48-49 0.7286110696362869 1107 50-51 0.6420150945408452 1107 52-53 0.8157293854908048 1107 54-55 1.0444069857333815 1107 56-57 0.9529339787723146 1107 58-59 0.8735041525304723 1107 60-61 0.9190037866428611 1107 62-63 0.8956104224340002 1107 64-65 0.9321748999715247 1107 66-67 1.1823953656258936 1107 68-69 0.8577413512704801 1107 70-71 0.6635568258454079 1107 72-73 1.0539601445254085 1107 74-75 0.8439823044470209 1107 76-77 0.6885581556595675 1107 78-79 1.0110019274604163 1107 80-81 1.0741632514995523 1107 82-83 1.226638835898914 1107 84-85 1.147820110241863 1107 86-87 1.3714657641045207 1107 88-89 1.3493739583923485 1107 90-91 1.3484126000181114 1107 92-93 1.4785748309640958 1107 94-95 1.677208081248125 1107 96-97 1.614451806462153 1107 98-99 1.9384432446716922 1107 100-101 2.2615862374207047 1108 1 0.1325076875452993 1108 2 0.15530529749259614 1108 3 0.08817228534992694 1108 4 0.11707402925513577 1108 5 -0.05391485555259834 1108 6 0.0971251385677192 1108 7 0.09412596844789789 1108 8 0.0709420903712612 1108 9 0.07583239675768993 1108 10-11 0.15227941757929386 1108 12-13 0.1057580474469546 1108 14-15 0.14274572963929444 1108 16-17 0.24569266218807684 1108 18-19 0.17503459233271457 1108 20-21 0.19006730485725143 1108 22-23 0.12634259130117442 1108 24-25 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-2.394145982227453 2316 92-93 -2.5420245328710465 2316 94-95 -2.6502424928630504 2316 96-97 -2.442888487497285 2316 98-99 -2.7346945254266863 2316 100-101 -2.7345926957008224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 57.0 5 117.0 6 296.0 7 632.0 8 1463.0 9 2829.0 10 4462.0 11 6808.0 12 10012.0 13 13859.0 14 17913.0 15 23685.0 16 30001.0 17 37369.0 18 32655.0 19 24668.0 20 24400.0 21 25796.0 22 28332.0 23 32206.0 24 36973.0 25 43912.0 26 52085.0 27 63049.0 28 75297.0 29 89431.0 30 106877.0 31 126690.0 32 151983.0 33 184537.0 34 226721.0 35 281099.0 36 334988.0 37 292390.0 38 86139.0 39 1638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.17270112919035 22.456245998541466 16.52647903215493 20.844573840113252 2 16.230884895020687 23.396661897331978 38.90870716500819 21.463746042639148 3 19.6571319436689 26.99402117352678 31.342346891706196 22.00649999109812 4 13.706585265979228 19.63283099915999 37.76513355204912 28.89545018281166 5 12.708140708804317 39.19837173684775 34.53852052222575 13.554967032122184 6 29.263460813240776 39.2083664455231 17.617694614703595 13.910478126532535 7 26.159883239843474 34.608242888316745 21.78963183081916 17.442242041020624 8 25.471150718055707 36.16118177742458 20.172606899096703 18.19506060542301 9 26.155956453743734 16.823170719371046 21.081615848538984 35.93925697834624 10-11 23.991035835616792 28.319480164247253 27.816889725483087 19.87259427465287 12-13 25.246002603120793 25.817066526063883 28.532235218043233 20.40469565277209 14-15 22.303667230667166 26.68098012672106 27.46399868734835 23.551353955263423 16-17 20.68606164289333 30.005086194199553 28.013216012326637 21.29563615058048 18-19 20.65581405417566 28.058257985952416 31.33103624220655 19.95489171766537 20-21 21.649849749359717 27.052014256866784 31.504300953661136 19.79383504011236 22-23 21.322460253216494 27.101557500545752 31.288598378992 20.287383867245754 24-25 21.058961937296232 27.411115033791816 31.375738073729988 20.154184955181968 26-27 20.978691756990294 27.557416046267065 31.27528181497788 20.188610381764764 28-29 20.678718302042956 27.555526871783066 31.471819441137157 20.293935385036825 30-31 21.051219634570906 27.23183577217087 31.46822686449278 20.248717728765442 32-33 20.899460890757293 27.427422972038222 31.28025244078083 20.392863696423653 34-35 21.131437454782642 27.431702568103212 31.210133867660222 20.226726109453928 36-37 21.20033099184255 27.24414897060728 31.110481678104364 20.44503835944581 38-39 20.964907009432494 27.640715015408556 31.103558748886922 20.29081922627202 40-41 21.223644032151263 27.346820936719375 31.01895883697391 20.410576194155453 42-43 21.05310116225809 27.518171915791722 30.746367639989707 20.68235928196048 44-45 21.2341338452207 27.594859060353805 30.454297341117925 20.71670975330757 46-47 21.44810785669039 27.898666762698976 29.866162389148943 20.78706299146169 48-49 21.414000406653333 28.1562924545575 29.51562732346238 20.914079815326783 50-51 21.402183413181405 28.154558623591868 29.395555483620356 21.04770247960637 52-53 21.559225333947023 28.38290231701827 29.1399669214156 20.9179054276191 54-55 21.49004044677583 28.23910049219146 29.12926540303537 21.141593657997344 56-57 21.58298430558686 28.4762334055869 28.91117100545483 21.029611283371416 58-59 21.700465405354226 28.210536239871104 29.078912548950107 21.010085805824566 60-61 21.688156638496466 28.301191273663974 29.196856855159727 20.813795232679837 62-63 21.595729700946347 28.231169751832567 29.310377616537377 20.86272293068371 64-65 21.48287131010448 28.23778235324227 29.1873070042035 21.092039332449758 66-67 21.61466636670125 28.414386039307338 28.84038009287059 21.130567501120822 68-69 21.501287837236635 28.344011865380292 28.94604112703309 21.208659170349982 70-71 21.51150345939345 28.246425994795636 29.057772012234423 21.18429853357649 72-73 21.626149656670943 28.238885809200493 28.8957428815363 21.239221652592267 74-75 21.478342078940297 28.552653301695923 28.60137188953667 21.367632729827104 76-77 21.420744729492746 28.454244851286358 28.798119603753996 21.326890815466896 78-79 21.460446222841938 28.427703697739144 28.643999112239943 21.467850967178975 80-81 21.425208605578206 28.643124477891746 28.594042002540714 21.337624913989334 82-83 21.575972274767892 28.513632988656205 28.58161079066681 21.32878394590909 84-85 21.40639007408304 28.52230270235056 28.414487070520405 21.656820153046 86-87 21.27840711373528 28.568925640166576 28.558890793351754 21.59377645274639 88-89 21.497124532768186 28.38123081125099 28.47043352031036 21.65121113567046 90-91 21.41369676549129 28.503606003889754 28.5015625983136 21.58113463230535 92-93 21.53355836134719 28.406536224870475 28.428204140493875 21.631701273288456 94-95 21.335249389413917 28.381975207534527 28.388368355374855 21.894407047676697 96-97 21.466785897814592 28.371038160711176 28.24408531231412 21.91809062916011 98-99 21.219994990650967 28.31451590387391 28.195634906665468 22.269854198809654 100-101 21.250951655784963 28.564078628264184 28.09520543524684 22.08976428070401 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1440.0 1 1051.0 2 668.5 3 711.5 4 674.0 5 670.5 6 1062.0 7 2304.5 8 3796.0 9 4436.0 10 4091.0 11 3463.0 12 3327.0 13 3560.5 14 3874.0 15 4196.5 16 4502.0 17 4800.5 18 5144.5 19 5564.0 20 6165.0 21 7094.0 22 8349.5 23 9952.5 24 11806.0 25 14513.5 26 18647.5 27 22987.0 28 26263.5 29 30597.5 30 36707.5 31 41992.5 32 47802.5 33 55331.5 34 63717.5 35 70458.5 36 77852.5 37 85382.5 38 87635.0 39 87374.0 40 87172.0 41 87616.0 42 87443.5 43 88442.5 44 90827.0 45 91368.5 46 90270.0 47 89733.0 48 92386.0 49 90734.5 50 84280.5 51 78302.5 52 72331.5 53 68036.5 54 63181.5 55 56854.0 56 50576.0 57 44523.5 58 39137.0 59 34152.5 60 28879.0 61 22945.5 62 17513.5 63 13073.0 64 9498.5 65 6992.5 66 5036.5 67 3699.0 68 2548.0 69 1551.5 70 972.0 71 656.0 72 450.5 73 322.5 74 222.5 75 164.0 76 117.5 77 58.5 78 29.5 79 16.5 80 9.5 81 5.5 82 3.5 83 4.0 84 4.0 85 2.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017075369548222946 2 0.0021040739727668087 3 3.2370368811797054E-4 4 0.0 5 0.0020636110117520623 6 0.0 7 2.023148050737316E-4 8 0.0 9 4.046296101474632E-5 10-11 0.0 12-13 0.005320879373439141 14-15 0.002528935063421645 16-17 0.0 18-19 3.8439812964009005E-4 20-21 0.0010318055058760312 22-23 0.0013150462329792555 24-25 5.462499736990754E-4 26-27 2.023148050737316E-4 28-29 1.011574025368658E-4 30-31 0.0 32-33 1.8208332456635845E-4 34-35 0.0 36-37 0.0014566665965308676 38-39 0.008274675527515624 40-41 6.271758957285679E-4 42-43 0.0 44-45 7.687962592801801E-4 46-47 2.2254628558110478E-4 48-49 1.4162036355161212E-4 50-51 0.0014566665965308676 52-53 3.4393516862534374E-4 54-55 0.0 56-57 2.8324072710322423E-4 58-59 8.092592202949263E-5 60-61 0.002002916570229943 62-63 0.0 64-65 6.069444152211948E-5 66-67 0.0 68-69 1.8208332456635845E-4 70-71 3.641666491327169E-4 72-73 2.4277776608847793E-4 74-75 0.002427777660884779 76-77 8.092592202949263E-5 78-79 3.034722076105974E-4 80-81 0.0018410647261709577 82-83 4.046296101474632E-5 84-85 0.0019219906482004503 86-87 3.2370368811797054E-4 88-89 0.00236708321936266 90-91 0.001274583271964509 92-93 0.0 94-95 0.0 96-97 2.4277776608847793E-4 98-99 6.878703372506875E-4 100-101 0.0018410647261709577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2471396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.92836409820306 #Duplication Level Percentage of deduplicated Percentage of total 1 74.82396640394454 39.6031013709969 2 14.099829643503469 14.925618341879765 3 4.842043709553249 7.688443573159446 4 2.105367284112645 4.4573458469583604 5 1.1290543188551672 2.9879499037507466 6 0.6688374522356173 2.124028331666475 7 0.44010068237907246 1.6305666409779016 8 0.3104992046980684 1.3147371966769477 9 0.21851428230874395 1.0409043145225259 >10 1.1877558925769784 11.686191371138737 >50 0.10827915790566078 3.9706018467678414 >100 0.06261461908844868 5.90537315975352 >500 0.002372141804536144 0.7885170157290875 >1k 5.356449236041065E-4 0.5016878254570662 >5k 7.652070337201522E-5 0.3391161833012041 >10k+ 1.5304140674403044E-4 1.0358170772636206 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13921 0.5632848802862835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11654 0.4715553476658536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8373 0.33879637257647094 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2992 0.12106517935612099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.0462961014746324E-5 0.0 0.0 0.0 4 0.0 4.0462961014746324E-5 0.0 0.0 0.0 5 0.0 4.0462961014746324E-5 0.0 0.0 0.0 6 0.0 4.0462961014746324E-5 0.0 0.0 0.0 7 0.0 8.092592202949265E-5 0.0 0.0 0.0 8 0.0 8.092592202949265E-5 0.0 0.0 0.0 9 0.0 8.092592202949265E-5 0.0 0.0 0.0 10-11 0.0 8.092592202949265E-5 0.0 2.0231480507373162E-5 0.0 12-13 0.0 8.092592202949265E-5 0.0 4.0462961014746324E-5 0.0 14-15 0.0 1.0115740253686581E-4 0.0 4.0462961014746324E-5 6.069444152211948E-5 16-17 0.0 1.2138888304423896E-4 0.0 1.0115740253686581E-4 1.2138888304423896E-4 18-19 0.0 1.2138888304423896E-4 0.0 1.4162036355161212E-4 1.2138888304423896E-4 20-21 0.0 1.2138888304423896E-4 0.0 2.630092465958511E-4 1.2138888304423896E-4 22-23 0.0 1.2138888304423896E-4 0.0 5.462499736990753E-4 1.4162036355161212E-4 24-25 0.0 1.2138888304423896E-4 0.0 7.283332982654338E-4 1.618518440589853E-4 26-27 0.0 1.2138888304423896E-4 0.0 0.0012138888304423895 1.618518440589853E-4 28-29 0.0 1.2138888304423896E-4 0.0 0.0018612962066783308 1.618518440589853E-4 30-31 0.0 1.2138888304423896E-4 0.0 0.003884444257415647 1.618518440589853E-4 32-33 0.0 1.2138888304423896E-4 0.0 0.007566573709757562 1.618518440589853E-4 34-35 0.0 1.2138888304423896E-4 0.0 0.013919258589072735 1.618518440589853E-4 36-37 0.0 1.8208332456635845E-4 0.0 0.023670832193626597 1.618518440589853E-4 38-39 0.0 2.4277776608847793E-4 0.0 0.03732708153610348 1.618518440589853E-4 40-41 0.0 3.2370368811797054E-4 0.0 0.05209606230648589 1.618518440589853E-4 42-43 2.0231480507373162E-5 4.046296101474632E-4 0.0 0.06907027445217198 1.618518440589853E-4 44-45 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.08922082903751563 1.618518440589853E-4 46-47 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.11325582788027495 1.618518440589853E-4 48-49 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.13917235441021997 1.618518440589853E-4 50-51 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.17057161215766312 1.618518440589853E-4 52-53 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.20387262907279935 1.618518440589853E-4 54-55 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.24186734946564614 1.618518440589853E-4 56-57 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.2889662360868109 1.618518440589853E-4 58-59 4.0462961014746324E-5 4.046296101474632E-4 0.0 0.3440767889888953 1.618518440589853E-4 60-61 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.4060053508219646 1.618518440589853E-4 62-63 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.46829807930416656 1.618518440589853E-4 64-65 4.0462961014746324E-5 4.4509257116220956E-4 0.0 0.5363770112114772 1.618518440589853E-4 66-67 4.0462961014746324E-5 4.8555553217695586E-4 0.0 0.6188202942790229 1.618518440589853E-4 68-69 4.0462961014746324E-5 4.8555553217695586E-4 0.0 0.7132406138069334 1.618518440589853E-4 70-71 4.0462961014746324E-5 5.05787012684329E-4 0.0 0.8211957937942767 1.618518440589853E-4 72-73 4.0462961014746324E-5 5.260184931917022E-4 0.0 0.9350383346092654 1.618518440589853E-4 74-75 4.0462961014746324E-5 5.260184931917022E-4 0.0 1.0538173566680533 1.618518440589853E-4 76-77 4.0462961014746324E-5 5.260184931917022E-4 0.0 1.1859491558617072 1.618518440589853E-4 78-79 4.0462961014746324E-5 5.260184931917022E-4 0.0 1.3322227599300152 1.618518440589853E-4 80-81 4.0462961014746324E-5 5.664814542064485E-4 0.0 1.498808770427726 1.618518440589853E-4 82-83 4.0462961014746324E-5 5.664814542064485E-4 0.0 1.6716867713632295 1.618518440589853E-4 84-85 4.0462961014746324E-5 5.664814542064485E-4 0.0 1.850512827567901 1.618518440589853E-4 86-87 4.0462961014746324E-5 5.664814542064485E-4 0.0 2.0412349943109076 1.8208332456635845E-4 88-89 4.0462961014746324E-5 5.664814542064485E-4 0.0 2.2532406785476713 2.023148050737316E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9145 0.0 42.286083 1 GTATCAA 14690 0.0 35.896988 1 TATCAAC 19235 0.0 27.114738 2 ATCAACG 19220 0.0 27.110638 3 TCAACGC 19385 0.0 26.928885 4 CAACGCA 19755 0.0 26.376434 5 AACGCAG 20235 0.0 25.77631 6 ACGCAGA 23065 0.0 22.469475 7 CGCAGAG 23125 0.0 22.329912 8 GAGTACT 13340 0.0 22.166462 12-13 GTACATG 12745 0.0 21.358541 1 AGTACTT 14035 0.0 21.288794 12-13 GCAGAGT 24155 0.0 21.004068 9 GTACTTT 14285 0.0 20.748697 14-15 AGAGTAC 20990 0.0 20.697147 10-11 TACATGG 13095 0.0 20.240627 2 ACATGGG 12980 0.0 19.4315 3 TCGTGCG 65 0.007566403 18.2693 82-83 CAGAGTA 23850 0.0 18.235144 10-11 ACTTTTT 16790 0.0 17.935797 16-17 >>END_MODULE