FastQCFastQC Report
Thu 26 May 2016
SRR936803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985528
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174420.8784565113158816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115930.5838749189132564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88410.44527198810593455No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.1365883533246572No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27070.13633653113932415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.11659367180921146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.11603966300147871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139250.034.990161
GGTATCA89150.034.2318951
ATCAACG169750.028.615653
TATCAAC170900.028.3432352
TCAACGC172750.028.1462214
CAACGCA174150.027.8100815
AACGCAG176400.027.4823046
GTACATG102100.026.5378911
TACATGG105950.025.5286942
ACGCAGA193750.024.7760017
GAGTACT102100.024.61815512-13
CGCAGAG194350.024.5766188
ACATGGG105600.024.5748713
ACCGTAA1201.70484E-423.763518
GCAGAGT197750.023.6018779
CATGGGA63650.022.775294
GTACTTT109600.022.76096714-15
AGTACTT106350.021.75790212-13
AGAGTAC169900.020.2792710-11
CATGGGG44800.020.1575664
Produced by FastQC (version 0.11.5)