##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936803_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985528 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02001180542405 33.0 31.0 34.0 30.0 34.0 2 32.12309773521199 33.0 31.0 34.0 30.0 34.0 3 32.12999212300204 34.0 31.0 34.0 30.0 34.0 4 35.73041327042479 37.0 35.0 37.0 35.0 37.0 5 35.75644211514519 37.0 35.0 37.0 35.0 37.0 6 35.82677101506501 37.0 35.0 37.0 35.0 37.0 7 35.77517617480086 37.0 35.0 37.0 35.0 37.0 8 35.75651715815642 37.0 35.0 37.0 35.0 37.0 9 37.41009796890298 39.0 37.0 39.0 35.0 39.0 10-11 37.299752005511884 39.0 37.0 39.0 34.0 39.0 12-13 37.20076297085713 39.0 37.0 39.0 34.0 39.0 14-15 38.375747156423884 40.0 38.0 41.0 34.0 41.0 16-17 38.32456580818805 40.0 38.0 41.0 34.0 41.0 18-19 38.31726296481339 40.0 38.0 41.0 34.0 41.0 20-21 38.27609079297799 40.0 38.0 41.0 34.0 41.0 22-23 38.143463350806435 40.0 38.0 41.0 34.0 41.0 24-25 37.98296448098441 40.0 38.0 41.0 33.0 41.0 26-27 37.92866154493918 40.0 37.5 41.0 33.0 41.0 28-29 37.98517724252692 40.0 38.0 41.0 33.0 41.0 30-31 37.82266706891064 40.0 37.0 41.0 33.0 41.0 32-33 37.605583250399896 40.0 37.0 41.0 33.0 41.0 34-35 37.40665707056259 40.0 36.0 41.0 33.0 41.0 36-37 37.24352489614853 40.0 36.0 41.0 32.0 41.0 38-39 37.07062302823229 39.0 36.0 41.0 32.0 41.0 40-41 36.86057184789134 39.0 35.5 41.0 31.0 41.0 42-43 36.62823616690372 39.0 35.0 41.0 31.0 41.0 44-45 36.327575083302776 39.0 35.0 41.0 30.0 41.0 46-47 35.91191008134864 39.0 35.0 40.0 29.5 41.0 48-49 35.519593025129836 39.0 35.0 40.0 27.5 41.0 50-51 34.83377293092819 38.0 34.0 39.5 26.5 40.5 52-53 35.123880146741826 38.5 35.0 40.0 27.5 40.5 54-55 35.418319459609734 39.0 35.0 40.5 28.0 41.0 56-57 35.28559859140742 39.0 35.0 41.0 27.5 41.0 58-59 35.03554772332599 38.0 35.0 40.0 26.5 41.0 60-61 34.736211979886455 38.0 34.5 40.0 26.0 41.0 62-63 34.418760400256254 37.5 34.0 40.0 26.0 41.0 64-65 34.09046283910375 37.0 34.0 40.0 26.0 41.0 66-67 33.70877670826097 36.0 34.0 39.0 25.0 41.0 68-69 33.264663354029764 36.0 33.0 39.0 23.0 41.0 70-71 32.90896527271335 35.0 33.0 38.5 23.0 40.0 72-73 32.54126937519894 35.0 33.0 37.5 22.0 40.0 74-75 32.125781656063275 35.0 32.5 37.0 21.0 39.0 76-77 31.701822638613002 35.0 32.0 36.5 20.0 39.0 78-79 31.300868836903838 35.0 32.0 36.0 18.5 38.5 80-81 30.91008462232716 35.0 31.0 35.5 17.5 37.0 82-83 30.53594484691226 35.0 31.0 35.0 12.0 37.0 84-85 30.212606923699894 34.0 31.0 35.0 7.5 36.5 86-87 29.898552928994203 34.0 31.0 35.0 4.5 36.0 88-89 29.551370466697023 34.0 30.0 35.0 2.0 36.0 90-91 29.238567020963693 34.0 30.0 35.0 2.0 35.0 92-93 28.903597934655167 34.0 29.0 35.0 2.0 35.0 94-95 28.532156433956107 34.0 29.0 35.0 2.0 35.0 96-97 28.122297947951377 34.0 29.0 35.0 2.0 35.0 98-99 27.57044272354759 34.0 28.5 35.0 2.0 35.0 100-101 25.44406198250541 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40152105625233503 1101 2 0.31323339779063986 1101 3 0.37776172756128545 1101 4 0.31816148962762725 1101 5 0.3287315518263725 1101 6 0.2742130824136808 1101 7 0.26129603628705667 1101 8 0.20962312775741054 1101 9 0.31477710126868885 1101 10-11 0.3156665461974626 1101 12-13 0.4925436060093489 1101 14-15 0.736044544286635 1101 16-17 0.6697713127779537 1101 18-19 0.726442954002195 1101 20-21 0.7485930992373397 1101 22-23 0.6967308826823029 1101 24-25 0.7381040500109961 1101 26-27 0.8048848338235146 1101 28-29 0.821492633113067 1101 30-31 0.7482999361952238 1101 32-33 0.8335491146178882 1101 34-35 0.991070920000034 1101 36-37 0.9287195740894347 1101 38-39 0.9112937827167897 1101 40-41 0.9751700415939197 1101 42-43 0.9350665837687444 1101 44-45 0.9997844914907859 1101 46-47 1.0202575628828612 1101 48-49 1.0411974822216337 1101 50-51 1.055537352507237 1101 52-53 1.2170442827979358 1101 54-55 1.1363876413466159 1101 56-57 1.2371578880413736 1101 58-59 1.2166927084291927 1101 60-61 1.275333513875978 1101 62-63 1.293000200022881 1101 64-65 1.2754917500699676 1101 66-67 1.2574820301149998 1101 68-69 1.1610349869789545 1101 70-71 1.1252460243627382 1101 72-73 1.1203087862920214 1101 74-75 1.1435289540719964 1101 76-77 1.1102277712613962 1101 78-79 1.1417899425406084 1101 80-81 1.1864333787756927 1101 82-83 1.111498118914156 1101 84-85 1.127344255968147 1101 86-87 1.2010700188976848 1101 88-89 1.0763707166713168 1101 90-91 1.1948926898611596 1101 92-93 1.0896593250584914 1101 94-95 1.1995898617151717 1101 96-97 1.0754299544522148 1101 98-99 1.1201421294489293 1101 100-101 1.358154178777525 1102 1 0.34929114070891387 1102 2 0.3760820807977794 1102 3 0.18453891103832376 1102 4 0.18947321578394138 1102 5 0.1889417944734788 1102 6 0.16135075957382128 1102 7 0.19893637676251075 1102 8 0.16350630140746603 1102 9 0.10169650168371192 1102 10-11 0.29010683999787545 1102 12-13 0.2987973707608731 1102 14-15 0.45022608730651115 1102 16-17 0.20483356718212065 1102 18-19 0.30902514384974467 1102 20-21 0.33372674594517093 1102 22-23 0.390756714790939 1102 24-25 0.31910474855965987 1102 26-27 0.3371327886370423 1102 28-29 0.4646456341916334 1102 30-31 0.3931678702888064 1102 32-33 0.4369301768533731 1102 34-35 0.3182875505040599 1102 36-37 0.41414909872944605 1102 38-39 0.44761782020189145 1102 40-41 0.5273052470271509 1102 42-43 0.5397077536721753 1102 44-45 0.6653828031475584 1102 46-47 0.5047525027414466 1102 48-49 0.5920357209013503 1102 50-51 0.3813037652470044 1102 52-53 0.6782499967052118 1102 54-55 0.6319680004605388 1102 56-57 0.6872508833171054 1102 58-59 0.528155233580101 1102 60-61 0.6175343481922226 1102 62-63 0.5444891493930868 1102 64-65 0.5357328597373794 1102 66-67 0.4962765724473641 1102 68-69 0.55486475634752 1102 70-71 0.5712288373850782 1102 72-73 0.5163925774401648 1102 74-75 0.5802836909572591 1102 76-77 0.6898409322019639 1102 78-79 0.6571572246028872 1102 80-81 0.642462657898708 1102 82-83 0.5980711621894805 1102 84-85 0.7252377493737185 1102 86-87 0.7974360284345288 1102 88-89 0.7909318885615946 1102 90-91 0.6063944196757838 1102 92-93 0.6315514497502157 1102 94-95 0.7148270528872835 1102 96-97 0.4490013199492715 1102 98-99 0.4866328054800384 1102 100-101 0.7677893999793497 1103 1 0.3047776247734646 1103 2 0.37022467329881437 1103 3 0.3394059147278625 1103 4 0.2476799595373791 1103 5 0.23743299397412443 1103 6 0.18731636562787202 1103 7 0.24032043141954773 1103 8 0.23789956754222885 1103 9 0.2809174504566485 1103 10-11 0.37415153157868986 1103 12-13 0.17670212652229367 1103 14-15 0.3483806051330802 1103 16-17 0.479499222368176 1103 18-19 0.49101952070764554 1103 20-21 0.438948604741654 1103 22-23 0.3655074239603948 1103 24-25 0.5804443140349989 1103 26-27 0.5366531336116651 1103 28-29 0.5515159952345954 1103 30-31 0.5375961584248188 1103 32-33 0.5480725963108739 1103 34-35 0.5014844446817648 1103 36-37 0.6971707670235432 1103 38-39 0.6422295035754999 1103 40-41 0.6505534710134526 1103 42-43 0.7919905376953125 1103 44-45 0.7841367236649575 1103 46-47 0.7003583173129542 1103 48-49 0.7728016045975608 1103 50-51 0.71707918178749 1103 52-53 0.7772768586226562 1103 54-55 0.8285163808040394 1103 56-57 0.7341833391755515 1103 58-59 0.707321430299686 1103 60-61 0.7501323302265916 1103 62-63 0.7713264235867285 1103 64-65 0.8026890009285381 1103 66-67 0.8057476347801185 1103 68-69 0.8942337229995658 1103 70-71 0.8464975376736916 1103 72-73 0.7720848596988787 1103 74-75 0.8050326619662869 1103 76-77 0.8541835174413457 1103 78-79 0.8429866593067636 1103 80-81 0.7834792268163859 1103 82-83 0.8665157480901406 1103 84-85 0.9049856504484453 1103 86-87 0.9359954178998855 1103 88-89 0.9518696106598696 1103 90-91 0.9579676866139657 1103 92-93 0.7905766159956009 1103 94-95 0.8808206008357935 1103 96-97 0.7859889102563642 1103 98-99 0.9453991982641234 1103 100-101 1.2373386640808484 1104 1 0.3529974654973955 1104 2 0.3287143358320108 1104 3 0.3703815857076478 1104 4 0.3794706308923139 1104 5 0.25126211298255896 1104 6 0.2858121091394352 1104 7 0.34230880920857487 1104 8 0.27096213758838417 1104 9 0.37712222072490675 1104 10-11 0.3816506192355291 1104 12-13 0.45979572998514584 1104 14-15 0.47123404530517377 1104 16-17 0.3913602924396571 1104 18-19 0.47038995874628853 1104 20-21 0.4965721599691548 1104 22-23 0.45890092022047924 1104 24-25 0.3857354526589205 1104 26-27 0.5355366180594103 1104 28-29 0.5731539468248243 1104 30-31 0.6894155612240382 1104 32-33 0.6234210446204003 1104 34-35 0.6345864743064951 1104 36-37 0.5544328615120477 1104 38-39 0.523218216446871 1104 40-41 0.6504960199097454 1104 42-43 0.7139184160348933 1104 44-45 0.6688469913654913 1104 46-47 0.7183760637133716 1104 48-49 0.6235495295815525 1104 50-51 0.6952619041880794 1104 52-53 0.7209393242551059 1104 54-55 0.6759964673676251 1104 56-57 0.7552938208888165 1104 58-59 0.8520041293270069 1104 60-61 0.7462432342300218 1104 62-63 0.7389793898510533 1104 64-65 0.5965183988448999 1104 66-67 0.5229543483687848 1104 68-69 0.6243729255507375 1104 70-71 0.5666042062049783 1104 72-73 0.628973276495735 1104 74-75 0.4813765169373099 1104 76-77 0.5653431340882982 1104 78-79 0.6946491862203992 1104 80-81 0.741687820872972 1104 82-83 0.5852601871179282 1104 84-85 0.6524964954490997 1104 86-87 0.5806231209988653 1104 88-89 0.6627167325854515 1104 90-91 0.6792651152007672 1104 92-93 0.6386880754579067 1104 94-95 0.47119786504762473 1104 96-97 0.47932493577133073 1104 98-99 0.453758579371371 1104 100-101 0.7411935840362389 1105 1 0.27560505562826876 1105 2 0.26241215175027577 1105 3 0.27166692480485466 1105 4 0.2690601887060211 1105 5 0.2859095572252883 1105 6 0.2674480838915727 1105 7 0.32351201786009653 1105 8 0.3100281950535404 1105 9 0.2396349669151192 1105 10-11 0.38057088366670655 1105 12-13 0.2948332779926659 1105 14-15 0.38710562315373664 1105 16-17 0.4397023861160818 1105 18-19 0.4446447351247045 1105 20-21 0.4613490813297716 1105 22-23 0.38946415420387126 1105 24-25 0.5074809386350339 1105 26-27 0.47340109569051236 1105 28-29 0.4551782221496623 1105 30-31 0.389185267834776 1105 32-33 0.4736204657759302 1105 34-35 0.2559923260809356 1105 36-37 0.38442147167895513 1105 38-39 0.44892937757178686 1105 40-41 0.5341050236639475 1105 42-43 0.5151152005669175 1105 44-45 0.44348800752566575 1105 46-47 0.4833009459015116 1105 48-49 0.5769815985800051 1105 50-51 0.4888090921397463 1105 52-53 0.4387085537134823 1105 54-55 0.532863538028586 1105 56-57 0.5719996425836342 1105 58-59 0.6599988489464863 1105 60-61 0.5548023926029941 1105 62-63 0.3537595014745847 1105 64-65 0.4875377294603638 1105 66-67 0.6480062149103816 1105 68-69 0.7154930764006409 1105 70-71 0.7790081465958067 1105 72-73 0.7886435578690012 1105 74-75 0.8420848821237357 1105 76-77 0.5982404912427945 1105 78-79 0.6724567463905444 1105 80-81 0.7118424158621615 1105 82-83 0.7436344489485514 1105 84-85 0.7205017052871554 1105 86-87 0.9237182625652984 1105 88-89 1.0171949700385028 1105 90-91 0.9893982026658925 1105 92-93 0.9096176863686871 1105 94-95 0.992674972632404 1105 96-97 0.9912493855896827 1105 98-99 0.9522704505180393 1105 100-101 1.1040421004450884 1106 1 0.4878478374866262 1106 2 0.4682042547482581 1106 3 0.4254203945394366 1106 4 0.29348098629903063 1106 5 0.40922881204502914 1106 6 0.4330001733770672 1106 7 0.45371357535908174 1106 8 0.37036487516278527 1106 9 0.5499634723308304 1106 10-11 0.5050332425292154 1106 12-13 0.4985873453255323 1106 14-15 0.7242751992220207 1106 16-17 0.6600714898787672 1106 18-19 0.7420036664114988 1106 20-21 0.6915325530240182 1106 22-23 0.7057867959400923 1106 24-25 0.7466066230260182 1106 26-27 0.7796463134947942 1106 28-29 0.6849306337970162 1106 30-31 0.6040703361537965 1106 32-33 0.7212461401317682 1106 34-35 0.6817423175679878 1106 36-37 0.6463758616246977 1106 38-39 0.7548597851090122 1106 40-41 0.713517672496927 1106 42-43 0.9318326630879099 1106 44-45 0.9681397261168527 1106 46-47 0.7726175573072496 1106 48-49 0.7894868726667639 1106 50-51 0.6500075089778576 1106 52-53 0.7560605303092771 1106 54-55 0.682107621298151 1106 56-57 0.7227758325540421 1106 58-59 0.8574757664307882 1106 60-61 0.9071087950004255 1106 62-63 0.9724562771724976 1106 64-65 0.8932431468805149 1106 66-67 1.053155527661076 1106 68-69 0.9220243972033515 1106 70-71 1.0076056149890533 1106 72-73 1.1250223873746563 1106 74-75 1.0014540844337532 1106 76-77 0.9405973331906701 1106 78-79 0.9455909599748757 1106 80-81 1.1398850424351714 1106 82-83 1.168908053122447 1106 84-85 1.2591181682855606 1106 86-87 1.2322263482956188 1106 88-89 1.3388822403155203 1106 90-91 1.2645516219315986 1106 92-93 1.361609571660484 1106 94-95 1.2841464332550778 1106 96-97 1.2443678902318922 1106 98-99 1.449092580687573 1106 100-101 1.568694951090393 1107 1 0.24435673417674053 1107 2 0.24167501660883062 1107 3 0.3284606703248656 1107 4 0.21676726833728566 1107 5 0.22853237140788707 1107 6 0.16365868546482432 1107 7 0.26382144495876503 1107 8 0.2897855950213071 1107 9 0.25625653272070537 1107 10-11 0.3900550298342438 1107 12-13 0.338902468455359 1107 14-15 0.41531264629316667 1107 16-17 0.3576178928478697 1107 18-19 0.43301482519677137 1107 20-21 0.5141495021476601 1107 22-23 0.5210429066047695 1107 24-25 0.44321730511703805 1107 26-27 0.5616774648767162 1107 28-29 0.48637746052855135 1107 30-31 0.5344044839503255 1107 32-33 0.6282682623164035 1107 34-35 0.6861767487806603 1107 36-37 0.5976337087372343 1107 38-39 0.7068711113340882 1107 40-41 0.7542889092281584 1107 42-43 0.7438488017271183 1107 44-45 0.8100024240575863 1107 46-47 0.8055053870861073 1107 48-49 0.6962849523430066 1107 50-51 0.6350998009919238 1107 52-53 0.7095943272164007 1107 54-55 0.7366379337048201 1107 56-57 0.6600843099578384 1107 58-59 0.6507101089737901 1107 60-61 0.7118700772340674 1107 62-63 0.6544433305469539 1107 64-65 0.7309869914546923 1107 66-67 0.65922864272585 1107 68-69 0.6664044471889881 1107 70-71 0.723561870334045 1107 72-73 0.6601829697241328 1107 74-75 0.7490057805847457 1107 76-77 0.6552426082975877 1107 78-79 0.6465881149192185 1107 80-81 0.6555404271642331 1107 82-83 0.6119510477324006 1107 84-85 0.7602645506977055 1107 86-87 0.8049409311656497 1107 88-89 0.8608644575927009 1107 90-91 0.8482093550808685 1107 92-93 0.8184000372070805 1107 94-95 0.7961370888351915 1107 96-97 0.7970878633846894 1107 98-99 0.9337031576254553 1107 100-101 1.0735318772745472 1108 1 0.11255991467258752 1108 2 0.18316685571677027 1108 3 0.07705094935437984 1108 4 0.09717354239991494 1108 5 0.061730197669923825 1108 6 0.1011958913175377 1108 7 0.09534311692853237 1108 8 -0.16324522516720208 1108 9 0.009447660331289853 1108 10-11 -0.001853791803931415 1108 12-13 -0.04774213783055359 1108 14-15 0.18811575920592105 1108 16-17 0.11843363689462194 1108 18-19 0.21046768428944773 1108 20-21 0.24229323424250993 1108 22-23 0.15063507293245237 1108 24-25 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1008.0 6 1162.0 7 1395.0 8 1611.0 9 2044.0 10 2720.0 11 3805.0 12 5477.0 13 7308.0 14 8202.0 15 10878.0 16 15092.0 17 21597.0 18 18363.0 19 10446.0 20 9445.0 21 9484.0 22 10438.0 23 11426.0 24 12932.0 25 14640.0 26 17405.0 27 21046.0 28 25637.0 29 32138.0 30 40786.0 31 52028.0 32 68566.0 33 92854.0 34 131954.0 35 198306.0 36 297364.0 37 419818.0 38 345479.0 39 56542.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.958385825581274 18.965793531611254 14.715836365285575 22.359984277521896 2 15.457196982299287 20.794177930618694 39.930798150181325 23.817826936900698 3 18.525891495058772 26.061234044762106 30.17856359895032 25.234310861228803 4 12.752889754413104 17.00390343129815 37.30320851866774 32.93999829562101 5 13.611951495071214 37.4832308319776 33.54337750831716 15.361440164634033 6 30.630124224072176 36.42743244203826 17.78525130911432 15.157192024775243 7 27.602016868408967 33.61092751040978 21.242659963266416 17.544395657914837 8 26.447744972458352 33.53850310754072 20.21935522247814 19.79439669752279 9 27.19244508645747 16.243769994630284 20.62989545439649 35.93388946451576 10-11 24.537737723001595 26.709548647697563 27.27493640049488 21.477777228805962 12-13 26.094123215653482 24.570845711632817 27.829517326342764 21.505513746370944 14-15 23.309573918413125 25.114214061574526 26.915073534044943 24.66113848596741 16-17 21.457942087657518 28.578583978442314 27.251949191208535 22.711524742691637 18-19 21.530654909445488 26.63729881640624 30.490991173767227 21.34105510038104 20-21 22.53566165575717 25.781446867358397 30.427793714759165 21.255097762125267 22-23 22.34700082378405 25.723482230179222 30.414817730534168 21.51469921550256 24-25 22.01083765836834 25.931967564631115 30.51136452397906 21.545830253021478 26-27 21.777916293572638 26.11523831071422 30.454777476391147 21.652067919321997 28-29 21.64603317032657 26.087356405253608 30.61228706627388 21.65432335814594 30-31 21.758356709125202 26.09471516469541 30.441377948133706 21.705550178045684 32-33 21.623046106876693 26.263084845249324 30.2344734733098 21.879395574564185 34-35 22.024576553644348 26.080461168747966 30.110598774170256 21.784363503437426 36-37 22.199855537530638 25.904181626661515 30.030532624105078 21.86543021170277 38-39 21.967544601545754 26.223061014042877 29.9755794680088 21.833814916402574 40-41 21.842929541464805 26.22320188736723 30.070176579099016 21.863691992068944 42-43 21.73252788923674 26.3009328957563 29.7299074663156 22.236631748691362 44-45 21.900287604778228 26.383858660491327 29.509746718342818 22.206107016387627 46-47 22.125845743277946 26.583223607230007 29.13901262377092 22.151918025721127 48-49 22.259019708908536 26.753846983252693 28.566454900573728 22.420678407265044 50-51 22.177817900074658 26.963761975817135 28.491638967840476 22.366781156267727 52-53 22.27456477100709 27.105633673310788 28.218648989552285 22.40115256612984 54-55 22.083054632852267 27.053937518186387 28.21471714892872 22.648290700032625 56-57 22.1791118479066 27.375073693545705 27.92368120046156 22.522133258086136 58-59 22.342477081001707 26.891456692377737 28.134421505989703 22.631644720630852 60-61 22.260327640531646 26.886331581973373 28.504126621964744 22.349214155530237 62-63 22.228294542294467 26.907810842946372 28.619046071271903 22.24484854348726 64-65 22.099152583790616 26.997302919930533 28.430665190737926 22.47287930554093 66-67 22.054251026019365 27.146174499891153 28.13613704716061 22.663437426928876 68-69 21.93851160979031 27.088637318167535 28.501599982061453 22.471251089980694 70-71 22.015277727750963 27.0504998245633 28.311251147013433 22.6229713006723 72-73 22.05941504772512 27.13308504231752 27.992003709505052 22.815496200452305 74-75 22.01200924345998 27.503676376128418 27.838970947182606 22.645343433228998 76-77 22.202535631735074 27.198800833873925 27.95351971633171 22.645143818059292 78-79 21.981968498163514 27.24693925322541 27.981609188451394 22.789483060159686 80-81 21.985690356773702 27.46122865626852 27.76529786816496 22.787783118792813 82-83 21.973059934841928 27.290925995449516 27.879288754059782 22.85672531564877 84-85 22.00101193814881 27.2536663859647 27.608676260928856 23.136645414957634 86-87 22.049889457629288 27.184150436399207 27.80892775160295 22.957032354368554 88-89 22.164254451294724 27.01030969407635 27.740375963840208 23.08505989078872 90-91 21.983714050228574 27.050742346921417 27.866400483584407 23.09914311926559 92-93 21.98722231028069 27.15434202306617 27.695124615612187 23.163311051040957 94-95 21.924344758064514 26.982409274193547 27.744027217741934 23.34921875 96-97 22.0112026970352 26.959816675807335 27.59324491079782 23.43573571635965 98-99 21.85999360130374 27.099461909478865 27.504082383340396 23.536462105876993 100-101 22.05856957491012 26.966216221326366 27.554543902979507 23.420670300784007 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2739.0 1 1891.0 2 1265.5 3 1412.5 4 1187.0 5 1291.5 6 2305.5 7 4706.0 8 7428.5 9 7909.0 10 6008.0 11 4528.0 12 4464.5 13 4473.5 14 4360.0 15 4244.0 16 4151.0 17 4250.0 18 4393.0 19 4582.5 20 4837.0 21 5280.5 22 5977.0 23 6903.0 24 7777.0 25 9157.5 26 11249.5 27 13343.0 28 15014.5 29 17043.5 30 20177.5 31 23811.5 32 27744.5 33 31850.5 34 36188.5 35 39772.0 36 44624.5 37 49621.5 38 51792.5 39 52185.5 40 52480.0 41 54067.0 42 55750.5 43 58637.5 44 62824.0 45 66010.0 46 67158.5 47 69169.0 48 75361.0 49 75616.5 50 71286.0 51 69020.0 52 67918.0 53 68556.0 54 67505.5 55 64008.0 56 59868.5 57 55592.5 58 51782.5 59 47365.0 60 40573.0 61 33152.0 62 26516.0 63 20532.0 64 15174.5 65 11261.5 66 8130.5 67 6075.0 68 4471.0 69 3051.0 70 2113.5 71 1343.5 72 800.0 73 572.5 74 405.0 75 273.0 76 194.0 77 100.0 78 52.5 79 36.5 80 31.0 81 30.0 82 21.0 83 12.5 84 9.5 85 9.5 86 8.5 87 3.5 88 3.0 89 4.5 90 5.0 91 4.5 92 3.0 93 2.5 94 1.5 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05580379626980833 2 0.08894359585964036 3 0.06572559037193129 4 0.12092501339694027 5 0.09957049208069592 6 0.09745518572389812 7 0.10359964704602503 8 0.0909078089052383 9 0.1099455661164184 10-11 0.10183689174869355 12-13 0.08629946291364311 14-15 0.08828885817777438 16-17 0.10138361181509402 18-19 0.0812126547699151 20-21 0.06668251467619696 22-23 0.0700821141781934 24-25 0.05796946706367273 26-27 0.050213343755414176 28-29 0.06318218630006728 30-31 0.04658710428661796 32-33 0.04696483756461757 34-35 0.041878029420889556 36-37 0.04681374425341773 38-39 0.0467130153792845 40-41 0.059253760208871395 42-43 0.05618152954780794 44-45 0.043313415877288056 46-47 0.03331607511956517 48-49 0.039838269719691684 50-51 0.024477116414374413 52-53 0.03724450121076107 54-55 0.043842242466487505 56-57 0.048249130709816235 58-59 0.052328650112211966 60-61 0.04910532613994867 62-63 0.05608080067367471 64-65 0.03973754084555846 66-67 0.05842274699727226 68-69 0.04908014392141535 70-71 0.02425047644757465 72-73 0.018005286251314513 74-75 0.02805299144610401 76-77 0.04107219842782373 78-79 0.05880048027527187 80-81 0.039410172004625474 82-83 0.05655926282580754 84-85 0.06129351991006926 86-87 0.06149497765833571 88-89 0.08395751659004556 90-91 0.05940485352007123 92-93 0.06882300325152806 94-95 0.07695685983778622 96-97 0.08662683175457611 98-99 0.11641739627947831 100-101 0.11052475714268446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1985528.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.52624227571529 #Duplication Level Percentage of deduplicated Percentage of total 1 65.51299153233472 26.549953670462813 2 15.831259140249973 12.831628868948053 3 6.7448683477931395 8.200325063414049 4 3.579968488133572 5.803306811581093 5 2.1186209800201885 4.292987356335576 6 1.3349092470024693 3.2459313336068845 7 0.93906218452927 2.663966312153696 8 0.6765940208214648 2.1935850568088835 9 0.47825666680072965 1.7443750993868172 >10 2.5176246189071354 18.341856967095428 >50 0.18220734399329794 5.069415045274994 >100 0.07990428073885437 5.6931266736805135 >500 0.0021154509159679127 0.5537736150125758 >1k 0.001244382891745831 0.9056752893189008 >5k 1.244382891745831E-4 0.4458530671456319 >10k+ 2.488765783491662E-4 1.4642397697741683 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17442 0.8784565113158816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11593 0.5838749189132564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8841 0.44527198810593455 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2712 0.1365883533246572 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2707 0.13633653113932415 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2315 0.11659367180921146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2304 0.11603966300147871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.036443706661402E-5 0.0 2 5.036443706661402E-5 0.0 0.0 5.036443706661402E-5 0.0 3 5.036443706661402E-5 0.0 0.0 1.0072887413322804E-4 0.0 4 5.036443706661402E-5 0.0 0.0 1.0072887413322804E-4 0.0 5 5.036443706661402E-5 0.0 0.0 1.0072887413322804E-4 0.0 6 1.0072887413322804E-4 0.0 0.0 1.0072887413322804E-4 0.0 7 1.0072887413322804E-4 0.0 0.0 2.0145774826645609E-4 0.0 8 1.0072887413322804E-4 0.0 0.0 2.0145774826645609E-4 0.0 9 1.0072887413322804E-4 0.0 0.0 3.5255105946629814E-4 0.0 10-11 1.0072887413322804E-4 0.0 0.0 4.0291549653291217E-4 0.0 12-13 1.7627552973314907E-4 0.0 0.0 4.5327993359952615E-4 1.2591109266653504E-4 14-15 2.518221853330701E-4 0.0 0.0 4.5327993359952615E-4 1.7627552973314907E-4 16-17 2.518221853330701E-4 0.0 0.0 7.051021189325963E-4 2.0145774826645609E-4 18-19 3.2736884093299113E-4 0.0 0.0 9.569243042656663E-4 2.0145774826645609E-4 20-21 4.0291549653291217E-4 0.0 0.0 0.0012087464895987365 2.0145774826645609E-4 22-23 4.5327993359952615E-4 0.0 0.0 0.0016116619861316487 2.0145774826645609E-4 24-25 8.058309930658243E-4 0.0 0.0 0.002316764105064245 2.0145774826645609E-4 26-27 8.058309930658243E-4 0.0 0.0 0.002921137349863613 2.0145774826645609E-4 28-29 9.317420857323593E-4 0.0 0.0 0.00513717258079463 2.0145774826645609E-4 30-31 9.569243042656663E-4 0.0 0.0 0.011508273869721303 2.0145774826645609E-4 32-33 9.569243042656663E-4 0.0 0.0 0.021178245786511195 3.021866223996841E-4 34-35 0.0010576531783988944 0.0 0.0 0.033064252934232104 3.021866223996841E-4 36-37 0.0011583820525321224 0.0 0.0 0.04588200216768537 3.021866223996841E-4 38-39 0.0013094753637319645 0.0 0.0 0.06308145742593406 3.021866223996841E-4 40-41 0.0013094753637319645 0.0 0.0 0.08579581854297699 3.021866223996841E-4 42-43 0.0014102042378651926 0.0 0.0 0.11271561015508216 3.021866223996841E-4 44-45 0.0016116619861316487 0.0 0.0 0.145099943188915 3.2736884093299113E-4 46-47 0.0017375730787981838 0.0 0.0 0.18473675516034022 3.7773327799960516E-4 48-49 0.0017627552973314908 0.0 0.0 0.23044248179829244 4.0291549653291217E-4 50-51 0.0017627552973314908 0.0 0.0 0.28342586959237037 4.0291549653291217E-4 52-53 0.0017627552973314908 0.0 0.0 0.3482449000971026 4.0291549653291217E-4 54-55 0.0017627552973314908 0.0 0.0 0.4207445072544935 4.0291549653291217E-4 56-57 0.0017627552973314908 0.0 0.0 0.506666236890137 4.2809771506621913E-4 58-59 0.0018131197343981046 0.0 0.0 0.6059345423484332 4.5327993359952615E-4 60-61 0.0018383019529314116 0.0 0.0 0.7254745337260415 4.5327993359952615E-4 62-63 0.0019138486085313326 0.0 0.0 0.8389707926556563 5.036443706661402E-4 64-65 0.0019138486085313326 0.0 0.0 0.962716214528327 5.036443706661402E-4 66-67 0.0021404885753310956 0.0 0.0 1.114967907780701 5.036443706661402E-4 68-69 0.002216035230931017 0.0 0.0 1.2926284595331823 5.036443706661402E-4 70-71 0.0022412174494643236 0.0 0.0 1.4826534805855167 5.288265891994472E-4 72-73 0.002266399667997631 0.0 0.0 1.6805353538202432 6.043732447993682E-4 74-75 0.002291581886530938 0.0 0.0 1.9028187968137442 6.799199003992893E-4 76-77 0.002316764105064245 0.0 0.0 2.1270916350713764 7.051021189325963E-4 78-79 0.002316764105064245 0.0 0.0 2.3718376169965874 7.051021189325963E-4 80-81 0.002392310760664166 0.0 0.0 2.645517968016568 7.051021189325963E-4 82-83 0.002518221853330701 0.0 0.0 2.963695299184902 7.051021189325963E-4 84-85 0.0025937685089306222 0.0 0.0 3.2956724861094884 7.051021189325963E-4 86-87 0.002669315164530543 0.0 0.0 3.6562566732879116 7.051021189325963E-4 88-89 0.002719679601597157 0.0 0.0 4.053757992836163 7.051021189325963E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13925 0.0 34.99016 1 GGTATCA 8915 0.0 34.231895 1 ATCAACG 16975 0.0 28.61565 3 TATCAAC 17090 0.0 28.343235 2 TCAACGC 17275 0.0 28.146221 4 CAACGCA 17415 0.0 27.810081 5 AACGCAG 17640 0.0 27.482304 6 GTACATG 10210 0.0 26.537891 1 TACATGG 10595 0.0 25.528694 2 ACGCAGA 19375 0.0 24.776001 7 GAGTACT 10210 0.0 24.618155 12-13 CGCAGAG 19435 0.0 24.576618 8 ACATGGG 10560 0.0 24.574871 3 ACCGTAA 120 1.70484E-4 23.76351 8 GCAGAGT 19775 0.0 23.601877 9 CATGGGA 6365 0.0 22.77529 4 GTACTTT 10960 0.0 22.760967 14-15 AGTACTT 10635 0.0 21.757902 12-13 AGAGTAC 16990 0.0 20.27927 10-11 CATGGGG 4480 0.0 20.157566 4 >>END_MODULE