FastQCFastQC Report
Thu 26 May 2016
SRR936803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985528
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164060.8262789545148697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118050.5945521795713785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94720.477051947894968No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.18372946641900795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34040.17144054377475412No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.12540744829586892No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.1218315732641393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98900.035.3532521
GTATCAA149100.032.8176461
TATCAAC186700.026.2298952
ATCAACG187650.025.9698893
TCAACGC188700.025.8002094
CAACGCA190800.025.5162435
AACGCAG195650.024.9079956
GTACATG105850.024.0110261
GAGTACT116550.023.71821412-13
TACATGG108200.023.0469932
ACGCAGA217000.022.3698187
ACATGGG107600.022.292073
AGTACTT120400.022.19051212-13
GTACTTT124100.022.08387814-15
CGCAGAG219100.022.0470188
GCAGAGT222050.021.1124029
AGAGTAC189900.020.07203310-11
TACTTTT133400.019.86779414-15
ACTTTTT140550.019.82021916-17
CATGGGG46150.019.657714
Produced by FastQC (version 0.11.5)