##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936803_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985528 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782156685778293 31.0 31.0 34.0 30.0 34.0 2 31.77450431321039 31.0 31.0 34.0 30.0 34.0 3 31.94342411690996 31.0 31.0 34.0 30.0 34.0 4 35.77533532642199 37.0 35.0 37.0 35.0 37.0 5 35.679536627033215 37.0 35.0 37.0 33.0 37.0 6 35.72313460197993 37.0 35.0 37.0 33.0 37.0 7 35.642537400630964 37.0 35.0 37.0 33.0 37.0 8 35.62093609357309 37.0 35.0 37.0 33.0 37.0 9 37.224562937415136 39.0 37.0 39.0 33.0 39.0 10-11 37.13820001531079 39.0 37.0 39.0 33.0 39.0 12-13 37.02110093637562 39.0 37.0 39.0 33.0 39.0 14-15 38.07282294684336 40.0 37.0 41.0 33.0 41.0 16-17 37.98774985797229 40.0 37.0 41.0 33.0 41.0 18-19 38.06520280751518 40.0 37.0 41.0 33.0 41.0 20-21 38.15614410877107 40.0 37.0 41.0 33.0 41.0 22-23 38.06738106941831 40.0 37.0 41.0 33.0 41.0 24-25 37.94542988061613 40.0 37.0 41.0 33.0 41.0 26-27 37.734740582857555 40.0 37.0 41.0 32.5 41.0 28-29 37.52528319922963 39.0 36.5 41.0 32.0 41.0 30-31 37.32500423061271 39.0 36.0 41.0 32.0 41.0 32-33 37.244082178644675 39.0 36.0 41.0 32.0 41.0 34-35 37.11635141886692 39.0 35.5 41.0 31.0 41.0 36-37 36.910872322122884 39.0 35.0 41.0 31.0 41.0 38-39 36.708196258123785 39.0 35.0 41.0 30.5 41.0 40-41 36.476964817418846 39.0 35.0 40.0 30.0 41.0 42-43 36.189269554496335 39.0 35.0 40.0 30.0 41.0 44-45 35.77155648270888 38.0 35.0 40.0 28.5 41.0 46-47 35.39909409487048 38.0 35.0 40.0 27.5 41.0 48-49 34.98481587769097 38.0 34.0 40.0 26.0 41.0 50-51 34.68041196094943 38.0 34.0 40.0 24.5 41.0 52-53 34.37986973741997 38.0 33.5 40.0 24.0 41.0 54-55 34.09131626448985 38.0 33.0 40.0 23.5 41.0 56-57 33.77844885592145 37.0 33.0 40.0 23.0 41.0 58-59 33.40130761187956 37.0 32.5 40.0 22.0 41.0 60-61 33.00836276295273 36.0 32.0 39.0 21.5 40.5 62-63 32.59139407754512 36.0 31.5 39.0 20.0 40.0 64-65 32.14091113295808 35.0 31.0 39.0 18.0 40.0 66-67 31.725425680222088 35.0 31.0 38.0 17.5 40.0 68-69 31.253964184841514 35.0 30.0 37.5 14.0 40.0 70-71 30.74429345745817 34.0 29.5 37.0 11.0 39.0 72-73 30.37093584175091 34.0 29.0 36.5 8.0 39.0 74-75 29.899479382814043 34.0 29.0 36.0 7.0 39.0 76-77 28.58213180574638 32.5 27.0 34.5 6.5 36.5 78-79 29.321234704320464 34.0 29.0 35.0 2.0 37.0 80-81 29.152337060973203 34.0 29.0 35.0 2.0 37.0 82-83 28.799786505151275 34.0 29.0 35.0 2.0 36.0 84-85 28.395627762489376 33.5 28.0 35.0 2.0 36.0 86-87 27.990119756558457 33.0 27.0 35.0 2.0 35.5 88-89 27.564272828184745 33.0 26.5 35.0 2.0 35.0 90-91 27.063286944329164 33.0 25.5 35.0 2.0 35.0 92-93 26.500023167641054 32.0 25.0 34.0 2.0 35.0 94-95 25.91723788332373 32.0 24.0 34.0 2.0 35.0 96-97 25.13303791233365 32.0 20.0 34.0 2.0 35.0 98-99 24.209133540297593 31.0 9.5 34.0 2.0 35.0 100-101 21.145876059164113 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35693509272137547 1101 2 0.1958829028527198 1101 3 0.27129025923004946 1101 4 0.2737347035303799 1101 5 0.3717248578897383 1101 6 0.23317258478171254 1101 7 0.34215035532915294 1101 8 0.33522480115925646 1101 9 0.4215156846465149 1101 10-11 0.46438320905593145 1101 12-13 0.34085352964730475 1101 14-15 0.5085038673627196 1101 16-17 0.4204121552234312 1101 18-19 0.4388734463917956 1101 20-21 0.5075399337170268 1101 22-23 0.5032934122675172 1101 24-25 0.46922202715638406 1101 26-27 0.5213665413101722 1101 28-29 0.4641269197280593 1101 30-31 0.5520680358340257 1101 32-33 0.6633608858149174 1101 34-35 0.6337245012066006 1101 36-37 0.5339343320922794 1101 38-39 0.5381767680173581 1101 40-41 0.5585511751919157 1101 42-43 0.5942607347928188 1101 44-45 0.636365381715116 1101 46-47 0.5450802339492711 1101 48-49 0.20951959859171865 1101 50-51 0.3659330639391527 1101 52-53 0.13965625613712263 1101 54-55 0.3165355336496418 1101 56-57 0.5448227243728709 1101 58-59 0.46031497045596126 1101 60-61 0.49331029229664125 1101 62-63 0.5696441221944539 1101 64-65 0.5119495321603722 1101 66-67 0.5873827695861955 1101 68-69 0.43962456189465016 1101 70-71 0.2135005589270058 1101 72-73 0.3593583862896246 1101 74-75 0.49653451242303603 1101 76-77 0.2543570484704034 1101 78-79 0.45070874143119966 1101 80-81 0.7582297036939707 1101 82-83 0.7136209715541106 1101 84-85 0.68867548296042 1101 86-87 0.5863061299440346 1101 88-89 0.6520808897097119 1101 90-91 0.7834485284037989 1101 92-93 1.033873527843717 1101 94-95 1.111976984164098 1101 96-97 0.9081446799233994 1101 98-99 0.7948104654740753 1101 100-101 0.9678914818422655 1102 1 0.2812099739838736 1102 2 0.12843747795221816 1102 3 0.20716077227697838 1102 4 0.15253651124185552 1102 5 0.2078714395742054 1102 6 0.2428671156030049 1102 7 0.22082325479491516 1102 8 0.2697551809300407 1102 9 0.38915596993189894 1102 10-11 0.3662540737424749 1102 12-13 0.35338956088173745 1102 14-15 0.44298546304969477 1102 16-17 0.4517570624410254 1102 18-19 0.4156064935777408 1102 20-21 0.28114443296959735 1102 22-23 0.37837806989983136 1102 24-25 0.39242779783058523 1102 26-27 0.4199087546213889 1102 28-29 0.4237895782812586 1102 30-31 0.3228706532627399 1102 32-33 0.4305751628564778 1102 34-35 0.32057818601135324 1102 36-37 0.4683906980308521 1102 38-39 0.597160864603147 1102 40-41 0.5522825682524726 1102 42-43 0.6068936443189443 1102 44-45 0.595291745540699 1102 46-47 0.7000384905421271 1102 48-49 0.6953392973485251 1102 50-51 0.7528370702655067 1102 52-53 0.7518527501482097 1102 54-55 0.809772679881263 1102 56-57 0.726081515363326 1102 58-59 0.5941808617283542 1102 60-61 0.5891504192653514 1102 62-63 0.8185026731869343 1102 64-65 0.6676837020701072 1102 66-67 0.6140050818295641 1102 68-69 0.41901895332157935 1102 70-71 0.4201446160597726 1102 72-73 0.4667934551668367 1102 74-75 0.2263963440712331 1102 76-77 0.310347773384148 1102 78-79 0.39915795946668453 1102 80-81 0.35508542658933706 1102 82-83 0.5302532298373528 1102 84-85 0.5278771309360479 1102 86-87 0.36867668556616806 1102 88-89 0.665895655262279 1102 90-91 0.8493875599283705 1102 92-93 0.825784870507853 1102 94-95 1.1776761359714953 1102 96-97 1.1675126037674062 1102 98-99 1.142334283933426 1102 100-101 1.1793667401318189 1103 1 0.05024205810774873 1103 2 -0.15906198128676152 1103 3 0.03255193562388925 1103 4 0.16903667501519237 1103 5 0.24469378876368353 1103 6 0.16050457705446775 1103 7 0.2875589586185612 1103 8 0.32498233145936695 1103 9 0.21356372073685748 1103 10-11 0.2576835523252541 1103 12-13 0.40954836539816597 1103 14-15 0.30258854832307946 1103 16-17 0.44435336811623927 1103 18-19 0.46991859920845513 1103 20-21 0.37180736349261423 1103 22-23 0.40165580398755907 1103 24-25 0.47726740811184953 1103 26-27 0.4594404130890908 1103 28-29 0.35600779157594786 1103 30-31 0.49208472278297677 1103 32-33 0.5507845030353309 1103 34-35 0.5020535288538426 1103 36-37 0.619300200129004 1103 38-39 0.8391808735334223 1103 40-41 0.6794921564088838 1103 42-43 0.6534723845311632 1103 44-45 0.7669142294569227 1103 46-47 0.696732412851766 1103 48-49 0.5367097557284666 1103 50-51 0.3929029619958797 1103 52-53 0.5773412504788453 1103 54-55 0.5734712187449915 1103 56-57 0.6492119500115834 1103 58-59 0.5895055964464504 1103 60-61 0.6571860739569928 1103 62-63 0.7840285787298882 1103 64-65 0.6062845370051235 1103 66-67 0.7021694067434971 1103 68-69 0.8413973438931812 1103 70-71 0.5316369851492553 1103 72-73 0.6697369139670251 1103 74-75 0.49380411727915074 1103 76-77 0.6074044111484262 1103 78-79 0.7331349593554428 1103 80-81 0.8977486707537565 1103 82-83 0.8119701190400583 1103 84-85 0.7489374358325591 1103 86-87 0.6211742289661046 1103 88-89 0.9510233946031015 1103 90-91 1.1879266515425577 1103 92-93 1.1608774796952304 1103 94-95 1.0777103777318437 1103 96-97 1.3996564817346417 1103 98-99 1.3614041054513883 1103 100-101 1.5177806841954968 1104 1 0.22393177029441347 1104 2 0.13323528899033477 1104 3 0.1476566459073041 1104 4 0.08616311418988687 1104 5 0.13670574802112867 1104 6 0.08861213180585992 1104 7 0.2307065248719553 1104 8 0.19111458173955498 1104 9 0.12982868797696057 1104 10-11 0.2629112099347424 1104 12-13 0.2775828855567255 1104 14-15 0.3768742985244984 1104 16-17 0.348398240079824 1104 18-19 0.38740359219450937 1104 20-21 0.4269053289182523 1104 22-23 0.3875609367968238 1104 24-25 0.370348252519328 1104 26-27 0.2573866490695451 1104 28-29 0.367454899438286 1104 30-31 0.3292156559106729 1104 32-33 0.36327951902932654 1104 34-35 0.2961103742166813 1104 36-37 0.2826211174768005 1104 38-39 0.3682046800923118 1104 40-41 0.5574235866997768 1104 42-43 0.5256612578335123 1104 44-45 0.38194282930379586 1104 46-47 0.5249721738865958 1104 48-49 0.5230113502568443 1104 50-51 0.4919350140157448 1104 52-53 0.5501354521795534 1104 54-55 0.5251889183595821 1104 56-57 0.40011643661869556 1104 58-59 0.4883736820342577 1104 60-61 0.43088057864598284 1104 62-63 0.5270667816515058 1104 64-65 0.3626121131813633 1104 66-67 0.47401231637379837 1104 68-69 0.43843873972127057 1104 70-71 0.4563728966655205 1104 72-73 0.5407920854895103 1104 74-75 0.6834849759070103 1104 76-77 0.5483130538764129 1104 78-79 0.6354684758775946 1104 80-81 0.7332205927789559 1104 82-83 0.7890621543333012 1104 84-85 0.8585290989212204 1104 86-87 0.6733644810949393 1104 88-89 0.8485192335718814 1104 90-91 0.9735618525507128 1104 92-93 1.0502259665019054 1104 94-95 1.0045551866858098 1104 96-97 1.181413094896815 1104 98-99 0.9884217194687501 1104 100-101 1.0439706291327866 1105 1 0.14429012598931124 1105 2 -0.04573905535118783 1105 3 0.051221734998861734 1105 4 0.14230123949628393 1105 5 0.100432260453438 1105 6 0.06953852074924072 1105 7 0.2531271378542286 1105 8 0.18582330280872128 1105 9 0.14292006067378082 1105 10-11 0.2789916212052148 1105 12-13 0.314066415279477 1105 14-15 0.3876221159685329 1105 16-17 0.4457081945323793 1105 18-19 0.35871629225470514 1105 20-21 0.28417544273335693 1105 22-23 0.29713798719465956 1105 24-25 0.2616149771259231 1105 26-27 0.27612171576586064 1105 28-29 0.3102545751014958 1105 30-31 0.46744756860606174 1105 32-33 0.5170899215057645 1105 34-35 0.5567085861634808 1105 36-37 0.3895001763928363 1105 38-39 0.5806031712762945 1105 40-41 0.42198860326734433 1105 42-43 0.3270929527191484 1105 44-45 0.5910446494181443 1105 46-47 0.46276214599854626 1105 48-49 0.5312791841237967 1105 50-51 0.5798318103973159 1105 52-53 0.666780480541199 1105 54-55 0.5914019336019081 1105 56-57 0.8110378558854379 1105 58-59 0.7213872162788562 1105 60-61 0.6950953796429289 1105 62-63 0.6030384346792985 1105 64-65 0.658645130222915 1105 66-67 0.6026508508660875 1105 68-69 0.5744730368310478 1105 70-71 0.3801658379016679 1105 72-73 0.5720166352165919 1105 74-75 0.561753955098176 1105 76-77 0.465132001105502 1105 78-79 0.6594667828585621 1105 80-81 1.0317448881172488 1105 82-83 1.0637429992417395 1105 84-85 1.0714897500994596 1105 86-87 1.013130949886044 1105 88-89 0.9588204934681386 1105 90-91 1.1462383999173973 1105 92-93 1.1006353901818997 1105 94-95 1.1940984163336132 1105 96-97 1.41700816611894 1105 98-99 1.2716657452555857 1105 100-101 1.226183288492365 1106 1 0.3788118229790136 1106 2 0.22684965247669453 1106 3 0.28164323121809787 1106 4 0.2551056960911424 1106 5 0.2519218474724312 1106 6 0.3172143708102553 1106 7 0.29767233962483175 1106 8 0.35733491301588316 1106 9 0.46673354055174343 1106 10-11 0.47349193545169754 1106 12-13 0.46774456507910145 1106 14-15 0.6406094872509414 1106 16-17 0.717841261855547 1106 18-19 0.6332629801282224 1106 20-21 0.5868475865574467 1106 22-23 0.695608518946635 1106 24-25 0.6404820299989424 1106 26-27 0.666242446405306 1106 28-29 0.6712737350045899 1106 30-31 0.7212471097858781 1106 32-33 0.7293714908755362 1106 34-35 0.6383547191275198 1106 36-37 0.6256840986536432 1106 38-39 0.7571090118411306 1106 40-41 0.7524578622477236 1106 42-43 0.6517403640924968 1106 44-45 0.7370251695180841 1106 46-47 0.5877596372132885 1106 48-49 0.5574794715573006 1106 50-51 0.7091035951663187 1106 52-53 0.738055915395428 1106 54-55 0.9754511784068072 1106 56-57 0.8458612610816516 1106 58-59 0.7907002736661042 1106 60-61 0.77953274762163 1106 62-63 0.8509993278474468 1106 64-65 0.9940404031528232 1106 66-67 0.9155997683900843 1106 68-69 0.9838243675696781 1106 70-71 1.0050254304929815 1106 72-73 1.1147436723896824 1106 74-75 1.0284853202359159 1106 76-77 0.9635351733827129 1106 78-79 1.0348266103172783 1106 80-81 0.9477332807289471 1106 82-83 1.2066863669944787 1106 84-85 1.185718745703614 1106 86-87 1.2595836402848022 1106 88-89 1.5895845826370198 1106 90-91 1.6690548152432463 1106 92-93 1.7221241901752222 1106 94-95 1.7373869703870533 1106 96-97 2.0278377295091197 1106 98-99 2.2197028829447127 1106 100-101 2.0835715314113337 1107 1 0.263774994511504 1107 2 0.21135993131488462 1107 3 0.15887480472831328 1107 4 0.1788320108784447 1107 5 0.22652760015461126 1107 6 0.25118293333599695 1107 7 0.20276049656258266 1107 8 0.22330610058090627 1107 9 0.31298827688379305 1107 10-11 0.3041101151513246 1107 12-13 0.21971115385191808 1107 14-15 0.3924915272124494 1107 16-17 0.3851193087922127 1107 18-19 0.2650750506196644 1107 20-21 0.2921945054874513 1107 22-23 0.31166194621678045 1107 24-25 0.3748422169347023 1107 26-27 0.3649037332097933 1107 28-29 0.35146027762844056 1107 30-31 0.38534640561506706 1107 32-33 0.40478599911999424 1107 34-35 0.4869819140513769 1107 36-37 0.4702733181354901 1107 38-39 0.506957262118874 1107 40-41 0.5060456803981523 1107 42-43 0.44925886832218964 1107 44-45 0.5743749266356346 1107 46-47 0.34054992560999864 1107 48-49 0.3118315446491593 1107 50-51 0.28292737791802125 1107 52-53 0.3979719397161219 1107 54-55 0.393106320146849 1107 56-57 0.4143975459026805 1107 58-59 0.4089345629220347 1107 60-61 0.45257313945445077 1107 62-63 0.2998430340410394 1107 64-65 0.3637966302201363 1107 66-67 0.3491710462352451 1107 68-69 0.35163218267445373 1107 70-71 0.5917907469127641 1107 72-73 0.5871703393216023 1107 74-75 0.6250414837376823 1107 76-77 0.6554668958362022 1107 78-79 0.7130210455845081 1107 80-81 0.8613766168971537 1107 82-83 0.6037095857085184 1107 84-85 0.6525786904792668 1107 86-87 0.7399345675863245 1107 88-89 0.748944053157782 1107 90-91 1.0709986800428268 1107 92-93 1.3639197700959933 1107 94-95 1.431077047601196 1107 96-97 1.333530824902681 1107 98-99 1.2660989839419337 1107 100-101 1.0381904898126066 1108 1 0.03411224093111187 1108 2 -0.13193871135595003 1108 3 -0.02931831074544533 1108 4 0.11460613337428072 1108 5 0.09247062497072278 1108 6 0.08805372563902125 1108 7 0.03016198654379565 1108 8 0.052481326990637456 1108 9 0.11494660973773563 1108 10-11 0.10439730065666453 1108 12-13 0.06297890261498651 1108 14-15 0.03466486461952201 1108 16-17 0.0919673376117629 1108 18-19 0.18047123196221548 1108 20-21 0.08179431645935153 1108 22-23 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-1.6336471011123699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 29.0 5 79.0 6 198.0 7 424.0 8 939.0 9 1701.0 10 2881.0 11 4688.0 12 6703.0 13 9195.0 14 11432.0 15 15139.0 16 19862.0 17 26232.0 18 23014.0 19 15106.0 20 13906.0 21 14859.0 22 15430.0 23 17097.0 24 19843.0 25 23622.0 26 29210.0 27 35585.0 28 43769.0 29 53201.0 30 64426.0 31 78602.0 32 97143.0 33 121554.0 34 159189.0 35 216753.0 36 294607.0 37 357755.0 38 183216.0 39 8124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37780322083595 20.148635368347033 15.572159025941676 21.901402384875336 2 15.995440882570996 22.006253424878317 38.6054802082326 23.392825484318085 3 18.923002614477184 26.299041593405736 30.448863072612557 24.329092719504526 4 13.50170508887565 17.92929534576966 36.59224981579198 31.97674974956271 5 13.588848770311603 38.00200755173878 33.45323577954805 14.955907898401563 6 30.80004613394053 37.10780484537323 17.14263697776354 14.949512042922697 7 27.493190728108598 33.94517730296425 20.952310921830364 17.609321047096792 8 26.64354267479482 34.02576040227083 19.87254775555923 19.458149167375126 9 27.141798050694828 16.207779492407056 20.43834184156557 36.212080615332546 10-11 24.67690559657602 27.086543543991986 26.98916383135657 21.247387028075423 12-13 26.197604601928315 24.797640713457035 27.71053663393713 21.294218050677518 14-15 23.364817821758244 25.356580214431627 26.842633294519143 24.43596866929099 16-17 21.486753145762737 28.82339105769347 27.238094854366196 22.451760942177597 18-19 21.53535482753202 26.784160183084804 30.675467684162598 21.005017305220576 20-21 22.539331660192186 25.86491374660222 30.70151051102324 20.894244082182357 22-23 22.320158668122534 25.735774061106163 30.683198625252327 21.260868645518975 24-25 22.085662458816124 26.030399573296908 30.72622378089058 21.157714186996383 26-27 21.847558656952835 26.21759922638549 30.70763566584487 21.2272064508168 28-29 21.682621819315514 26.18213100131405 30.870311731129448 21.264935448240987 30-31 21.793617028778726 26.14583747848509 30.681661915798053 21.37888357693813 32-33 21.773552615144105 26.248494091196626 30.495182099126065 21.4827711945332 34-35 22.116692185265023 26.10731410036868 30.427352579728833 21.348641134637468 36-37 22.348442876598625 25.96045946120186 30.230057981642506 21.46103968055701 38-39 22.13202295363767 26.228965733987312 30.25166955807423 21.387341754300785 40-41 21.984944902655105 26.21131179705461 30.31818379850718 21.485559501783104 42-43 21.91801382133599 26.365626263862506 29.854982243597394 21.861377671204107 44-45 22.121016995846666 26.442793542933078 29.59832325576066 21.83786620545959 46-47 22.380008526705637 26.6977549783395 29.102104655868356 21.82013183908651 48-49 22.48034334521853 26.900008990072394 28.571532897998942 22.04811476671014 50-51 22.387946569322185 27.05718786860509 28.475829578660765 22.079035983411952 52-53 22.57966467053805 27.18397288354126 28.178855810345954 22.05750663557474 54-55 22.35727888155055 27.16414257027202 28.14230232444523 22.3362762237322 56-57 22.396061285677664 27.443703692884636 27.934834232425647 22.225400789012053 58-59 22.593922043935464 26.98069456455115 28.111742968910146 22.31364042260324 60-61 22.50060815517199 27.007000205992103 28.484024028047163 22.00836761078875 62-63 22.45702402585106 26.996698107505914 28.611910786450757 21.93436708019227 64-65 22.348917048828685 27.059916440173083 28.4518919065929 22.139274604405337 66-67 22.258686111388116 27.243133557556032 28.163469025336358 22.334711305719495 68-69 22.159778698607802 27.12760211077409 28.458457513179745 22.254161677438365 70-71 22.294789109880035 27.11069543589112 28.259157718779093 22.33535773544975 72-73 22.348292834020178 27.18965111286547 27.947736810494945 22.514319242619404 74-75 22.290418777156 27.613183253345852 27.668433291245258 22.42796467825289 76-77 22.40189351997234 27.346244047809414 27.850346649233686 22.40151578298456 78-79 22.23286313804049 27.388332290366897 27.79109780926683 22.587706762325784 80-81 22.226587348956123 27.642811416955393 27.577838184416198 22.55276304967229 82-83 22.137359597460886 27.463558862984648 27.719112070960588 22.679969468593875 84-85 22.078598093749182 27.490168564791777 27.465338146975927 22.965895194483114 86-87 22.154478198657564 27.42645795989619 27.57563975165916 22.84342408978709 88-89 22.232527452049414 27.238224987817976 27.5342450229979 22.995002537134713 90-91 22.063288566824536 27.269645879464093 27.64675026674312 23.020315286968255 92-93 21.958130500902406 27.508522298745447 27.41439109475497 23.11895610559718 94-95 21.92857450440652 27.332212386305173 27.39154182763387 23.347671281654435 96-97 22.06924356779922 27.310672861248698 27.191559197853838 23.42852437309824 98-99 21.809993744591992 27.485215176969625 27.101127081484496 23.603663996953888 100-101 21.746128958173006 27.652061533750487 27.073470218261082 23.528339289815424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3856.0 1 2562.0 2 1385.0 3 1499.0 4 1246.5 5 1181.0 6 2092.0 7 4446.5 8 6813.5 9 7327.0 10 6035.0 11 4587.0 12 4296.5 13 4362.5 14 4383.0 15 4385.0 16 4354.0 17 4465.5 18 4616.0 19 4788.0 20 5112.5 21 5576.0 22 6247.5 23 7079.5 24 7903.5 25 9397.0 26 11610.0 27 13807.5 28 15446.0 29 17538.0 30 20639.0 31 23997.5 32 27755.5 33 31872.5 34 36391.0 35 40020.5 36 44781.0 37 49711.5 38 51817.5 39 52315.0 40 52734.0 41 54461.0 42 56344.0 43 59371.5 44 63235.0 45 66343.5 46 67247.5 47 69410.5 48 75833.5 49 75731.5 50 71007.5 51 68407.5 52 67526.0 53 68410.0 54 67294.0 55 63384.5 56 59068.0 57 54934.0 58 50993.5 59 46534.5 60 39983.5 61 32544.0 62 25806.0 63 20055.0 64 15004.0 65 10996.0 66 7916.5 67 6051.0 68 4490.5 69 3034.5 70 2066.5 71 1245.0 72 748.0 73 571.0 74 385.0 75 244.0 76 164.0 77 80.5 78 41.5 79 22.5 80 12.5 81 6.5 82 4.0 83 2.5 84 2.0 85 2.0 86 1.5 87 0.5 88 1.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013447304696785942 2 0.002820408475730385 3 0.002266399667997631 4 5.036443706661402E-5 5 0.0012591109266653505 6 2.518221853330701E-4 7 0.0 8 0.0 9 0.0 10-11 7.554665559992103E-5 12-13 6.295554633326752E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0020649419197311747 22-23 0.0 24-25 0.0036514216873295164 26-27 0.0021656707938644028 28-29 0.0033744172834631395 30-31 0.013950949067452083 32-33 8.058309930658243E-4 34-35 0.002417492979197473 36-37 0.0036262394687962096 38-39 0.002392310760664166 40-41 0.004406888243328726 42-43 0.002367128542130859 44-45 6.799199003992893E-4 46-47 7.554665559992103E-5 48-49 2.266399667997631E-4 50-51 1.0072887413322804E-4 52-53 9.065598671990524E-4 54-55 0.003273688409329911 56-57 3.5255105946629814E-4 58-59 0.0012591109266653505 60-61 7.554665559992102E-4 62-63 0.0 64-65 2.266399667997631E-4 66-67 1.0072887413322804E-4 68-69 2.518221853330701E-5 70-71 1.7627552973314907E-4 72-73 2.518221853330701E-5 74-75 4.532799335995262E-4 76-77 9.821065227989733E-4 78-79 2.770044038663771E-4 80-81 0.004784621521328332 82-83 0.0015864797675983415 84-85 0.003021866223996841 86-87 0.0015612975490650347 88-89 0.0010324709598655874 90-91 1.7627552973314907E-4 92-93 7.554665559992103E-5 94-95 2.266399667997631E-4 96-97 0.0014857508934651136 98-99 0.002316764105064245 100-101 8.058309930658243E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1985528.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.182120705248714 #Duplication Level Percentage of deduplicated Percentage of total 1 65.02074858592047 26.126715680250857 2 16.067905497153784 12.912850363343253 3 6.857897951427365 8.266946498055965 4 3.610363009798386 5.802881689979352 5 2.2062408663890514 4.432571839904868 6 1.3558881119317652 3.2689475865872377 7 0.9303004634712347 2.6167011860744984 8 0.6450932423038611 2.07369716227152 9 0.4896160167780256 1.7706428896857935 >10 2.549082372959547 18.4183924446408 >50 0.17974062395602627 4.982121516507975 >100 0.08322665476601612 5.821490701916019 >500 0.002262543761368514 0.575282364591818 >1k 0.0012569687563158411 1.0274775366893198 >5k 1.2569687563158412E-4 0.4784086529774978 >10k+ 2.5139375126316825E-4 1.4248718865232464 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16406 0.8262789545148697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11805 0.5945521795713785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9472 0.477051947894968 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3648 0.18372946641900795 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3404 0.17144054377475412 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2490 0.12540744829586892 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2419 0.1218315732641393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.036443706661402E-5 0.0 2 0.0 0.0 0.0 1.0072887413322804E-4 0.0 3 0.0 0.0 0.0 2.518221853330701E-4 0.0 4 0.0 0.0 0.0 3.021866223996841E-4 0.0 5 0.0 0.0 0.0 3.021866223996841E-4 0.0 6 0.0 0.0 0.0 3.021866223996841E-4 0.0 7 1.0072887413322804E-4 0.0 0.0 4.5327993359952615E-4 0.0 8 1.0072887413322804E-4 0.0 0.0 4.5327993359952615E-4 0.0 9 1.0072887413322804E-4 0.0 0.0 4.5327993359952615E-4 0.0 10-11 1.0072887413322804E-4 0.0 0.0 5.036443706661402E-4 0.0 12-13 2.0145774826645609E-4 0.0 0.0 6.043732447993682E-4 5.036443706661402E-5 14-15 2.518221853330701E-4 0.0 0.0 6.295554633326752E-4 5.036443706661402E-5 16-17 2.770044038663771E-4 0.0 0.0 0.0010072887413322803 5.036443706661402E-5 18-19 3.5255105946629814E-4 0.0 0.0 0.0013094753637319645 5.036443706661402E-5 20-21 4.0291549653291217E-4 0.0 0.0 0.0015864797675983417 5.036443706661402E-5 22-23 4.0291549653291217E-4 0.0 0.0 0.0021404885753310956 5.036443706661402E-5 24-25 4.0291549653291217E-4 0.0 0.0 0.002820408475730385 5.036443706661402E-5 26-27 4.2809771506621913E-4 0.0 0.0 0.003424781720529753 7.554665559992103E-5 28-29 5.288265891994472E-4 0.0 0.0 0.005766728044127305 1.0072887413322804E-4 30-31 5.540088077327542E-4 0.0 0.0 0.012288922644253821 1.0072887413322804E-4 32-33 5.540088077327542E-4 0.0 0.0 0.022009258998110327 1.5109331119984206E-4 34-35 6.043732447993682E-4 0.0 0.0 0.03379453727169801 2.0145774826645609E-4 36-37 7.051021189325963E-4 0.0 0.0 0.046511557631018045 2.0145774826645609E-4 38-39 9.569243042656663E-4 0.0 0.0 0.06350955514100028 2.0145774826645609E-4 40-41 9.569243042656663E-4 0.0 0.0 0.08617355182097658 2.0145774826645609E-4 42-43 0.0010324709598655874 0.0 0.0 0.11284152124774871 2.0145774826645609E-4 44-45 0.0012087464895987365 0.0 0.0 0.14482293878504862 2.0145774826645609E-4 46-47 0.0013598398007985785 0.0 0.0 0.18413238191554085 2.0145774826645609E-4 48-49 0.0014605686749318066 0.0 0.0 0.2299388374276263 2.0145774826645609E-4 50-51 0.0014605686749318066 0.0 0.0 0.28251930972517136 2.518221853330701E-4 52-53 0.0014605686749318066 0.0 0.0 0.3469354247333707 2.518221853330701E-4 54-55 0.0014605686749318066 0.0 0.0 0.41862920089769573 2.518221853330701E-4 56-57 0.0014605686749318066 0.0 0.0 0.5033925484808071 2.770044038663771E-4 58-59 0.0015109331119984206 0.0 0.0 0.601653565197771 3.021866223996841E-4 60-61 0.0015612975490650347 0.0 0.0 0.7199596278672474 3.021866223996841E-4 62-63 0.0015612975490650347 0.0 0.0 0.8324989624925965 3.021866223996841E-4 64-65 0.0015612975490650347 2.518221853330701E-5 0.0 0.9553881889351346 3.5255105946629814E-4 66-67 0.0017123908602648767 5.036443706661402E-5 0.0 1.1054993936121777 3.5255105946629814E-4 68-69 0.0017123908602648767 5.036443706661402E-5 0.0 1.2806417235113279 3.5255105946629814E-4 70-71 0.0017123908602648767 5.036443706661402E-5 0.0 1.4684507093327317 3.7773327799960516E-4 72-73 0.0017375730787981838 5.036443706661402E-5 0.0 1.6643431873033268 4.2809771506621913E-4 74-75 0.0017627552973314908 5.036443706661402E-5 0.0 1.8846372350326965 4.5327993359952615E-4 76-77 0.0017627552973314908 5.036443706661402E-5 0.0 2.1068199491520643 4.5327993359952615E-4 78-79 0.0017627552973314908 5.036443706661402E-5 0.0 2.3492491669722106 4.5327993359952615E-4 80-81 0.0020649419197311747 5.036443706661402E-5 0.0 2.6185981764044626 4.5327993359952615E-4 82-83 0.0020649419197311747 5.036443706661402E-5 0.0 2.9325952592962676 5.036443706661402E-4 84-85 0.002090124138264482 5.036443706661402E-5 0.0 3.258956811487927 5.036443706661402E-4 86-87 0.0021153063567977888 5.036443706661402E-5 0.0 3.611910786450758 5.791910262660612E-4 88-89 0.0021153063567977888 5.036443706661402E-5 0.0 4.00120270275715 6.547376818659823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9890 0.0 35.353252 1 GTATCAA 14910 0.0 32.817646 1 TATCAAC 18670 0.0 26.229895 2 ATCAACG 18765 0.0 25.969889 3 TCAACGC 18870 0.0 25.800209 4 CAACGCA 19080 0.0 25.516243 5 AACGCAG 19565 0.0 24.907995 6 GTACATG 10585 0.0 24.011026 1 GAGTACT 11655 0.0 23.718214 12-13 TACATGG 10820 0.0 23.046993 2 ACGCAGA 21700 0.0 22.369818 7 ACATGGG 10760 0.0 22.29207 3 AGTACTT 12040 0.0 22.190512 12-13 GTACTTT 12410 0.0 22.083878 14-15 CGCAGAG 21910 0.0 22.047018 8 GCAGAGT 22205 0.0 21.112402 9 AGAGTAC 18990 0.0 20.072033 10-11 TACTTTT 13340 0.0 19.867794 14-15 ACTTTTT 14055 0.0 19.820219 16-17 CATGGGG 4615 0.0 19.65771 4 >>END_MODULE