FastQCFastQC Report
Thu 26 May 2016
SRR936802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848303
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164960.8924943583384326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112010.6060153557073705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81430.4405662924314898No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.13266223124671658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23530.12730596660828877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.11561957103353726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20960.11340132002166312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135400.037.8374331
GGTATCA81600.036.109641
TATCAAC166200.030.6832982
ATCAACG165450.030.678763
TCAACGC166750.030.4395854
CAACGCA168200.030.1779945
AACGCAG170950.029.6893146
CGCAGAG188000.026.669588
ACGCAGA189550.026.6513587
GTACATG101750.025.8759881
GCAGAGT194550.024.9899859
GAGTACT99850.024.65256512-13
TACATGG105750.024.1338862
ACATGGG104800.023.218923
GTACTTT107950.022.75751114-15
AGTACTT103200.022.5169512-13
CATGGGA61400.021.2087734
AGAGTAC166450.020.99948910-11
ACTTTTT124700.020.11792816-17
ATGGGAT26300.020.059145
Produced by FastQC (version 0.11.5)