##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936802_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1848303 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07676122367383 33.0 31.0 34.0 31.0 34.0 2 32.19614478794873 33.0 31.0 34.0 31.0 34.0 3 32.204850611615086 34.0 31.0 34.0 30.0 34.0 4 35.80720206589504 37.0 35.0 37.0 35.0 37.0 5 35.81856492144416 37.0 35.0 37.0 35.0 37.0 6 35.89860753350506 37.0 35.0 37.0 35.0 37.0 7 35.84568655680373 37.0 35.0 37.0 35.0 37.0 8 35.808325258358614 37.0 35.0 37.0 35.0 37.0 9 37.49030164426504 39.0 37.0 39.0 35.0 39.0 10-11 37.33618703210459 39.0 37.0 39.0 34.5 39.0 12-13 37.27462867289616 39.0 37.0 39.0 34.0 39.0 14-15 38.4706035752796 40.0 38.0 41.0 34.0 41.0 16-17 38.42733929447715 40.0 38.0 41.0 34.0 41.0 18-19 38.42528146088601 40.0 38.0 41.0 34.0 41.0 20-21 38.368041928190344 40.0 38.0 41.0 34.0 41.0 22-23 38.26651663715311 40.0 38.0 41.0 34.0 41.0 24-25 38.12450393685451 40.0 38.0 41.0 34.0 41.0 26-27 38.18666717524129 40.0 38.0 41.0 34.0 41.0 28-29 38.140680667617815 40.0 38.0 41.0 34.0 41.0 30-31 37.96628042047219 40.0 37.5 41.0 33.5 41.0 32-33 37.73084878399267 40.0 37.0 41.0 33.0 41.0 34-35 37.55465878700625 40.0 37.0 41.0 33.0 41.0 36-37 37.37602492664894 40.0 36.0 41.0 33.0 41.0 38-39 37.208734444514775 39.5 36.0 41.0 32.0 41.0 40-41 37.02495911114141 39.0 36.0 41.0 31.5 41.0 42-43 36.78389744538639 39.0 36.0 41.0 31.0 41.0 44-45 36.476698625712345 39.0 35.0 41.0 30.5 41.0 46-47 36.04853884887922 39.0 35.0 40.5 29.5 41.0 48-49 35.663824870705724 39.0 35.0 40.0 28.5 41.0 50-51 34.981558489057264 38.0 34.0 39.5 27.0 40.5 52-53 35.1920231693613 38.5 35.0 40.0 28.0 40.5 54-55 35.55237804624025 39.0 35.0 40.5 28.0 41.0 56-57 35.4279658151288 39.0 35.0 41.0 28.0 41.0 58-59 35.1838313306855 38.5 35.0 40.5 27.5 41.0 60-61 34.89485327892667 38.0 35.0 40.0 26.5 41.0 62-63 34.568350535599414 37.5 34.0 40.0 26.0 41.0 64-65 34.23272293557929 37.0 34.0 40.0 26.0 41.0 66-67 33.85628519782741 36.5 34.0 39.0 25.5 41.0 68-69 33.4636347503629 36.0 34.0 39.0 25.0 41.0 70-71 33.046366044961246 35.5 33.0 39.0 24.0 40.5 72-73 32.66381486152433 35.0 33.0 37.5 23.0 40.0 74-75 32.24970824588826 35.0 33.0 37.0 21.5 39.0 76-77 31.839086989524986 35.0 32.0 36.5 20.0 39.0 78-79 31.428765467566734 35.0 32.0 36.0 19.0 38.5 80-81 31.02975377954805 35.0 31.5 36.0 17.5 37.0 82-83 30.653487820990392 35.0 31.0 35.0 14.0 37.0 84-85 30.332241521006026 34.5 31.0 35.0 9.0 36.5 86-87 30.03367791969174 34.0 31.0 35.0 7.0 36.0 88-89 29.708661945579266 34.0 30.5 35.0 2.0 36.0 90-91 29.382605287120132 34.0 30.0 35.0 2.0 35.5 92-93 29.042987540462793 34.0 30.0 35.0 2.0 35.0 94-95 28.659160051138798 34.0 29.0 35.0 2.0 35.0 96-97 28.22472532912623 34.0 29.0 35.0 2.0 35.0 98-99 27.677448719176454 34.0 29.0 35.0 2.0 35.0 100-101 25.51990339246325 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3308317930053022 1101 2 0.3767907882717694 1101 3 0.31336088143532237 1101 4 0.26731262032495806 1101 5 0.2482700693598474 1101 6 0.11928948717331878 1101 7 0.14464890742517156 1101 8 0.20175750289198646 1101 9 0.19430004470621753 1101 10-11 0.30161078822685994 1101 12-13 0.3403964737899656 1101 14-15 0.48471379005702175 1101 16-17 0.5078744786865599 1101 18-19 0.2536876579310672 1101 20-21 0.3391208946196187 1101 22-23 0.43024484085602666 1101 24-25 0.43845439500222483 1101 26-27 0.5077841142076451 1101 28-29 0.49688652949535594 1101 30-31 0.5538911467057801 1101 32-33 0.5507196402351937 1101 34-35 0.45796113205806677 1101 36-37 0.41876131721970467 1101 38-39 0.38484350557046554 1101 40-41 0.3971138087588457 1101 42-43 0.47243243452413 1101 44-45 0.625035093100557 1101 46-47 0.5494162375078417 1101 48-49 0.5639807850027765 1101 50-51 0.42670376341075666 1101 52-53 0.5462921495150894 1101 54-55 0.5519349796825637 1101 56-57 0.6727619908701712 1101 58-59 0.654883307742189 1101 60-61 0.7271583849445662 1101 62-63 0.6274723429416298 1101 64-65 0.6534542195735469 1101 66-67 0.6711305300935066 1101 68-69 0.6063198740208904 1101 70-71 0.592378137368641 1101 72-73 0.6961160766151764 1101 74-75 0.5150700161844028 1101 76-77 0.521862368021381 1101 78-79 0.5159976168449276 1101 80-81 0.3394881781357988 1101 82-83 0.48707195364437794 1101 84-85 0.5316322264553257 1101 86-87 0.6215613141760059 1101 88-89 0.7234711304353638 1101 90-91 0.7389664365671678 1101 92-93 0.6542594504283983 1101 94-95 0.6811731209777285 1101 96-97 0.5959534804686442 1101 98-99 0.9432563186530878 1101 100-101 0.9175896044755056 1102 1 0.28713685636952846 1102 2 0.28070843571108384 1102 3 0.23442182025880243 1102 4 0.24002069567988116 1102 5 0.16635481121146967 1102 6 0.12467521321359243 1102 7 0.14265624434594315 1102 8 0.14723178864935704 1102 9 0.1633352945847406 1102 10-11 0.35897123087862326 1102 12-13 0.3254089410790826 1102 14-15 0.3826915435798881 1102 16-17 0.4654587115092923 1102 18-19 0.3464057174347701 1102 20-21 0.3743215666845785 1102 22-23 0.37264074687883664 1102 24-25 0.3751432449183625 1102 26-27 0.4095992768994634 1102 28-29 0.25860059276018177 1102 30-31 0.3562291081576845 1102 32-33 0.33230190162505124 1102 34-35 0.2987633639677014 1102 36-37 0.13691576160440633 1102 38-39 0.2678136866561758 1102 40-41 0.2803735738099107 1102 42-43 0.2097114246171614 1102 44-45 0.19931789411460699 1102 46-47 0.12260865915797581 1102 48-49 0.2656210068365894 1102 50-51 0.30488137451268926 1102 52-53 0.14911122312815195 1102 54-55 0.19077883430208686 1102 56-57 0.24471690981940242 1102 58-59 0.3074756050666423 1102 60-61 0.26235221175649315 1102 62-63 0.24673024211561767 1102 64-65 0.26700704677217857 1102 66-67 -0.11696384868390908 1102 68-69 -0.03915147522214113 1102 70-71 0.05146048854043528 1102 72-73 -0.01944312401006698 1102 74-75 -0.22308645598250365 1102 76-77 -0.03714976474319798 1102 78-79 -0.04659686500810878 1102 80-81 -0.09149083788983248 1102 82-83 -0.1870143390042749 1102 84-85 -0.07892089181536832 1102 86-87 -0.2150741427664613 1102 88-89 -0.18486964173695597 1102 90-91 -0.10147438582351498 1102 92-93 0.036836773513826415 1102 94-95 0.003965591358117138 1102 96-97 0.09905477691988906 1102 98-99 0.31459443070055215 1102 100-101 0.3565897689599886 1103 1 0.32802724095397906 1103 2 0.31914523437623643 1103 3 0.26237681249688904 1103 4 0.29818984595273434 1103 5 0.2304181282252955 1103 6 0.14029058467617972 1103 7 0.16964410073401837 1103 8 0.1699990006389811 1103 9 0.14659490780586282 1103 10-11 0.3164283745471437 1103 12-13 0.24903430243936242 1103 14-15 0.3096021879723807 1103 16-17 0.38482857583422714 1103 18-19 0.35749421791148706 1103 20-21 0.5267802067327025 1103 22-23 0.5235742117055793 1103 24-25 0.4339851205899663 1103 26-27 0.5340480144297217 1103 28-29 0.39011269873920185 1103 30-31 0.357380734604142 1103 32-33 0.47112333041635424 1103 34-35 0.3877297340526553 1103 36-37 0.324880699255921 1103 38-39 0.4340488492134469 1103 40-41 0.3719523029377996 1103 42-43 0.3423016785489068 1103 44-45 0.4799963071459601 1103 46-47 0.3748702576885563 1103 48-49 0.40071211123225225 1103 50-51 0.36529132095877515 1103 52-53 0.39643030070694607 1103 54-55 0.31746638210034206 1103 56-57 0.2918969541726142 1103 58-59 0.3370480178243369 1103 60-61 0.36262841145673974 1103 62-63 0.47913755445700446 1103 64-65 0.3208951811274048 1103 66-67 0.4394959336569144 1103 68-69 0.40623464135407517 1103 70-71 0.31772500277709526 1103 72-73 0.28062090157474984 1103 74-75 0.24276262673780025 1103 76-77 0.2809397268988221 1103 78-79 0.314269124716418 1103 80-81 0.22844912025125907 1103 82-83 0.1900870217975701 1103 84-85 0.17604848850474397 1103 86-87 0.22555225543494117 1103 88-89 0.08274508946977832 1103 90-91 0.2814587842948839 1103 92-93 0.4569951415127882 1103 94-95 0.431724018695931 1103 96-97 0.3820150943628704 1103 98-99 0.5445167798199151 1103 100-101 0.664632566012191 1104 1 0.3337422411731552 1104 2 0.2659049532803195 1104 3 0.3017622782728395 1104 4 0.276775159177852 1104 5 0.2730543892270063 1104 6 0.22310011368961824 1104 7 0.268410867034099 1104 8 0.22725727513795846 1104 9 0.31164614222126374 1104 10-11 0.44006999644206957 1104 12-13 0.42471490802092404 1104 14-15 0.6365798089348687 1104 16-17 0.560835210341061 1104 18-19 0.5927290365079045 1104 20-21 0.5487098939691535 1104 22-23 0.4119942460416297 1104 24-25 0.6074688689368486 1104 26-27 0.42792893226376094 1104 28-29 0.4688296961380374 1104 30-31 0.5867789486362724 1104 32-33 0.6425676809754179 1104 34-35 0.5404738815405494 1104 36-37 0.43247923362056184 1104 38-39 0.44920889123763885 1104 40-41 0.4919074143704094 1104 42-43 0.5809581848560725 1104 44-45 0.6370672059131692 1104 46-47 0.7543544936648203 1104 48-49 0.6943394375466099 1104 50-51 0.5193674602268601 1104 52-53 0.5182160423348776 1104 54-55 0.4798241408716635 1104 56-57 0.42132225648161636 1104 58-59 0.556162988672618 1104 60-61 0.6239518060925349 1104 62-63 0.5830873524665279 1104 64-65 0.5851535430926376 1104 66-67 0.6172824962151751 1104 68-69 0.6491672977969571 1104 70-71 0.610368283298726 1104 72-73 0.7537233132393908 1104 74-75 0.5867052658470087 1104 76-77 0.6116629434913108 1104 78-79 0.7085117245769226 1104 80-81 0.785205827326795 1104 82-83 0.7635659423307466 1104 84-85 0.6797430789899934 1104 86-87 0.727824559883743 1104 88-89 0.792176960837427 1104 90-91 0.8396080833225952 1104 92-93 0.7032231343078337 1104 94-95 0.9061033769845501 1104 96-97 1.0105299800881156 1104 98-99 1.2102041140405682 1104 100-101 1.2604528509501591 1105 1 0.0705049691533901 1105 2 0.16168794065529113 1105 3 0.22188027290104628 1105 4 0.17172981505916596 1105 5 0.21236589832639652 1105 6 0.06025499953518931 1105 7 0.10548841637447026 1105 8 0.19959701120956197 1105 9 0.16588557817183158 1105 10-11 0.20645260591210501 1105 12-13 0.1934122864412231 1105 14-15 0.20665882639055155 1105 16-17 0.19742308964412558 1105 18-19 0.16655951361022403 1105 20-21 0.08704121080105409 1105 22-23 0.08460144285324134 1105 24-25 0.2553481975707683 1105 26-27 0.17109780728178947 1105 28-29 0.20158690446490368 1105 30-31 0.014035550095265137 1105 32-33 0.15144582655255334 1105 34-35 0.1798608949304139 1105 36-37 -0.09521586369241675 1105 38-39 -0.11667989375472843 1105 40-41 -0.06464527788803309 1105 42-43 0.12681830634043223 1105 44-45 0.3027087910516073 1105 46-47 0.3381469991822428 1105 48-49 0.49812063872527546 1105 50-51 0.33363521454731426 1105 52-53 0.33072572271354517 1105 54-55 0.36906420424924846 1105 56-57 0.5946336841965945 1105 58-59 0.45174560419322773 1105 60-61 0.4837545703080153 1105 62-63 0.5267319428632149 1105 64-65 0.6460805414796482 1105 66-67 0.48875007677349913 1105 68-69 0.570087312463194 1105 70-71 0.4641156843767078 1105 72-73 0.5010195714947443 1105 74-75 0.5447983606771132 1105 76-77 0.6575564070226747 1105 78-79 0.6413009753566214 1105 80-81 0.5649629651346935 1105 82-83 0.34918309935985903 1105 84-85 0.2336555711365058 1105 86-87 0.30639695782458887 1105 88-89 0.43518962889785584 1105 90-91 0.43946975111096265 1105 92-93 0.3312819475482698 1105 94-95 0.4039261306626649 1105 96-97 0.5588341907945136 1105 98-99 0.7648779340143932 1105 100-101 0.48216819594021487 1106 1 0.17874540089457014 1106 2 0.21226886847420445 1106 3 0.08021014733926535 1106 4 0.16000999022694629 1106 5 0.03431907810033863 1106 6 0.047131407383226076 1106 7 0.06929627014282147 1106 8 0.18407562191296734 1106 9 0.10218949723879689 1106 10-11 0.22413672073365376 1106 12-13 0.27403929806910554 1106 14-15 0.258369030719642 1106 16-17 0.29934234892756706 1106 18-19 0.2035161700456669 1106 20-21 0.2842669659450294 1106 22-23 0.1651070877922649 1106 24-25 0.3452511552980724 1106 26-27 0.21698618590499308 1106 28-29 0.17965739841289263 1106 30-31 0.24751341305550767 1106 32-33 0.2926872267843592 1106 34-35 0.24211423351371764 1106 36-37 0.1542136684539841 1106 38-39 0.2608192551238844 1106 40-41 0.3160470474510859 1106 42-43 0.37212858866400467 1106 44-45 0.3795323505356052 1106 46-47 0.28721995977215187 1106 48-49 0.3251878754267423 1106 50-51 0.41892290689908407 1106 52-53 0.4612825237674443 1106 54-55 0.437067969836761 1106 56-57 0.4286663472704504 1106 58-59 0.5327177280525461 1106 60-61 0.600306788076054 1106 62-63 0.6840689200842505 1106 64-65 0.6872719293082881 1106 66-67 0.719570309055797 1106 68-69 0.7833059326227598 1106 70-71 0.6292855322275202 1106 72-73 0.6203567827880434 1106 74-75 0.5709840288368255 1106 76-77 0.5980521960538354 1106 78-79 0.5353264252739294 1106 80-81 0.677748373949445 1106 82-83 0.6568284456857576 1106 84-85 0.7077661576207639 1106 86-87 0.7279714246717184 1106 88-89 0.6112992457810833 1106 90-91 0.6241346646785573 1106 92-93 0.6395373474776029 1106 94-95 0.6022643952811357 1106 96-97 0.5925856784525294 1106 98-99 0.6859034192424147 1106 100-101 0.6695142806597154 1107 1 0.09745040458457765 1107 2 0.20135622397135222 1107 3 0.13966429639673805 1107 4 0.1449701261654326 1107 5 0.148648516535566 1107 6 0.12525364875386913 1107 7 0.06497851843124636 1107 8 0.18678253137547074 1107 9 0.1982591909690683 1107 10-11 0.32220463451029957 1107 12-13 0.27012921752022834 1107 14-15 0.27487700702690887 1107 16-17 0.31662203682463286 1107 18-19 0.3226898602836812 1107 20-21 0.3630754612889433 1107 22-23 0.28129088886137055 1107 24-25 0.34135888781437984 1107 26-27 0.21095589963551475 1107 28-29 0.33844734562082834 1107 30-31 0.33080144332960515 1107 32-33 0.3021457389153923 1107 34-35 0.2788932525189054 1107 36-37 0.31137946998442345 1107 38-39 0.12942298364646376 1107 40-41 0.33082772014007844 1107 42-43 0.28110281090386025 1107 44-45 0.3603714676659777 1107 46-47 0.13919519194516283 1107 48-49 0.24282150216455278 1107 50-51 0.1308815841955706 1107 52-53 0.24204529274754805 1107 54-55 0.3769857411201585 1107 56-57 0.5082662838649554 1107 58-59 0.48096479842339335 1107 60-61 0.48277528206256903 1107 62-63 0.3625756756328471 1107 64-65 0.4640848201951471 1107 66-67 0.4830049115437163 1107 68-69 0.45897669035673516 1107 70-71 0.43372344321044665 1107 72-73 0.47509980451975764 1107 74-75 0.4655859259959172 1107 76-77 0.3568091778170981 1107 78-79 0.46429930815167353 1107 80-81 0.46845996531682843 1107 82-83 0.3998785881113598 1107 84-85 0.5342355607500444 1107 86-87 0.34539097895275006 1107 88-89 0.32025833818962823 1107 90-91 0.5032812474900332 1107 92-93 0.48622717263221915 1107 94-95 0.5115593654352324 1107 96-97 0.46741698985264435 1107 98-99 0.4501849315130215 1107 100-101 0.4351256848095275 1108 1 0.11527228201913431 1108 2 0.09746046748780657 1108 3 0.10815963613148227 1108 4 0.06692006937871753 1108 5 0.04066622835940592 1108 6 0.058483498855707694 1108 7 0.09781930912761538 1108 8 0.130995214055055 1108 9 0.017201550105156116 1108 10-11 -0.00372225611191368 1108 12-13 0.1880537568907883 1108 14-15 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-2.0302234305642948 2316 94-95 -2.72990261766067 2316 96-97 -2.825201970296682 2316 98-99 -2.680910581714258 2316 100-101 -2.7123013756016583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2812.0 3 897.0 4 840.0 5 851.0 6 993.0 7 1168.0 8 1435.0 9 1910.0 10 2478.0 11 3368.0 12 4912.0 13 6579.0 14 7522.0 15 9937.0 16 14177.0 17 20030.0 18 17674.0 19 9669.0 20 8698.0 21 8797.0 22 9483.0 23 10136.0 24 11692.0 25 13139.0 26 15461.0 27 18422.0 28 22824.0 29 28365.0 30 35675.0 31 46276.0 32 60522.0 33 82534.0 34 117725.0 35 181309.0 36 276003.0 37 400763.0 38 338111.0 39 55096.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92950200861469 18.916159957392036 14.796037113094046 22.358300920899225 2 15.437501420828204 20.696351962691487 40.07525788708502 23.790888729395284 3 18.444680515997337 26.06731430606238 30.228661696175912 25.259343481764375 4 12.618555562652439 17.014353870153716 37.42463920461004 32.9424513625838 5 13.471329510312627 37.39453487062656 33.773258634280715 15.360876984780102 6 30.474701910458364 36.52522662601186 17.797992091369373 15.202079372160407 7 27.469162537680607 33.652463532102146 21.329118447039257 17.54925548317799 8 26.342152977909112 33.55494786012947 20.26549348123035 19.837405680731067 9 27.128119476033604 16.254165392122097 20.677074169019498 35.9406409628248 10-11 24.46042990184122 26.68471076606298 27.374683702621045 21.48017562947476 12-13 26.08599840979312 24.600560010547234 27.836687864913088 21.476753714746557 14-15 23.266996397789892 25.115582299551097 26.97453602629692 24.64288527636209 16-17 21.45104689261484 28.588322386896216 27.27198907566778 22.68864164482116 18-19 21.456336378340108 26.66634549387354 30.666137633728773 21.211180494057583 20-21 22.48600100905333 25.759577886574608 30.52156256022381 21.232858544148247 22-23 22.244555841941835 25.677186288636776 30.568925510252402 21.509332359168983 24-25 21.937653479887572 25.919824108937306 30.644704815358757 21.497817595816365 26-27 21.687660034068454 26.106764773998886 30.606062409769464 21.599512782163195 28-29 21.567867748023186 26.04660475246721 30.771501430610325 21.614026068899275 30-31 21.644335068131127 26.04112217500138 30.604922799874895 21.709619956992597 32-33 21.5582705205715 26.176506633790574 30.41146789102517 21.853754954612757 34-35 21.939081943724496 26.046738256457097 30.296295374439563 21.717884425378845 36-37 22.134331854957175 25.83772093805916 30.15814314367663 21.869804063307036 38-39 21.85188301430546 26.139354664864495 30.205631011278633 21.80313130955141 40-41 21.76506697349494 26.115158537710414 30.232098850936058 21.887675637858592 42-43 21.64936293575738 26.21782383929917 29.860656653525087 22.27215657141837 44-45 21.83436591553548 26.337419837589454 29.62858962310344 22.199624623771626 46-47 22.05502502367104 26.555768970647907 29.2067090491005 22.18249695658055 48-49 22.16664840656985 26.74684878069894 28.70984976674417 22.376653045987037 50-51 22.121283033874896 26.999901255141832 28.527064883487828 22.351750827495437 52-53 22.213895838563623 27.051392483962843 28.34919013444374 22.38552154302979 54-55 22.02440518945066 27.059270712014165 28.306583936293826 22.609740162241348 56-57 22.079784837715092 27.29121993389821 28.105384062784527 22.523611165602176 58-59 22.257263534718223 26.877351017457592 28.214265616816082 22.651119831008106 60-61 22.176069239575195 26.848571709189674 28.675261186589218 22.300097864645917 62-63 22.098939889175767 26.823809705467056 28.76744091038096 22.30980949497622 64-65 22.10522613581947 26.93349710380577 28.575467435738577 22.38580932463618 66-67 21.97399891774892 27.073863636363637 28.330167748917752 22.621969696969696 68-69 21.8296765904687 26.96592941917576 28.691038217682518 22.51335577267302 70-71 21.912806036709117 26.965294983307274 28.536838314455316 22.585060665528296 72-73 21.98378291651549 27.090332493772284 28.128650081615724 22.7972345080965 74-75 21.9090595722736 27.48021193133823 27.97243117669946 22.638297319688718 76-77 22.112928504013368 27.1576459662825 28.08589995583995 22.643525573864178 78-79 21.92774077477893 27.22781146115118 28.103191278622297 22.741256485447593 80-81 21.905056736646326 27.38453436928598 27.901175702703384 22.809233191364306 82-83 21.8736867115026 27.26092635734328 27.958767104715893 22.906619826438227 84-85 21.861359530970798 27.16023360546176 27.807859940624557 23.170546922942883 86-87 22.00695507611782 27.077742403505894 27.925940236782136 22.989362283594147 88-89 22.075688259569397 26.883521057706982 27.88421264533893 23.156578037384694 90-91 21.851944947778748 26.96503079485994 28.029802681830102 23.153221575531216 92-93 21.904308838082283 27.07244663777168 27.830513785732787 23.192730738413257 94-95 21.888591669647578 26.922741817737077 27.83460299404341 23.354063518571934 96-97 21.915409697826828 26.902718906343072 27.690227238100036 23.49164415773007 98-99 21.792388253167683 27.05296056736099 27.602333039840264 23.552318139631065 100-101 22.01269285272474 26.869651251081084 27.650143941659977 23.467511954534196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2216.0 1 1541.0 2 1055.5 3 1224.0 4 1060.5 5 1129.0 6 1971.0 7 4098.5 8 6842.5 9 7511.5 10 5700.5 11 4239.5 12 4022.5 13 4035.5 14 4036.0 15 4015.0 16 3953.0 17 3976.0 18 4093.0 19 4275.0 20 4556.5 21 4988.5 22 5587.0 23 6403.5 24 7290.0 25 8655.5 26 10801.0 27 12769.0 28 14194.5 29 16184.0 30 19031.5 31 22321.0 32 26156.0 33 30147.5 34 33970.0 35 37394.5 36 42000.0 37 46508.0 38 48334.0 39 48725.5 40 49270.5 41 50873.5 42 52310.0 43 54771.0 44 58898.0 45 62064.5 46 63001.5 47 64583.0 48 70317.0 49 70675.0 50 66341.0 51 63988.0 52 63040.0 53 63734.0 54 62834.5 55 59277.5 56 55061.0 57 51210.0 58 47758.5 59 43434.5 60 37042.0 61 30251.5 62 24245.5 63 18714.5 64 13733.5 65 10104.5 66 7201.0 67 5450.0 68 4039.5 69 2736.5 70 1921.5 71 1172.5 72 719.5 73 568.0 74 386.0 75 232.5 76 161.5 77 95.5 78 45.5 79 24.5 80 19.0 81 14.0 82 14.0 83 11.5 84 10.0 85 8.5 86 5.5 87 4.5 88 6.0 89 5.5 90 2.0 91 0.5 92 1.5 93 2.0 94 1.0 95 0.5 96 1.5 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041172902927712605 2 0.042687806057772996 3 0.025104109012429238 4 0.04636685651649107 5 0.06746729297090359 6 0.06530314564224589 7 0.06487031617651436 8 0.0677378113869858 9 0.0669803598219556 10-11 0.07285060945093959 12-13 0.07431140889778354 14-15 0.06722382639642958 16-17 0.05683591921887267 18-19 0.049072040677313196 20-21 0.055564482663286265 22-23 0.030595632858898134 24-25 0.018449355976806834 26-27 0.014012853953058563 28-29 0.017069712054787554 30-31 0.01347181712089414 32-33 0.008196708007291012 34-35 0.009792766662176062 36-37 0.005626783054510001 38-39 0.008791848522671878 40-41 0.0079261895912088 42-43 0.0053292127968195685 44-45 0.0012984883971946157 46-47 0.002867495210471443 48-49 5.680886737726444E-4 50-51 0.005707938579334665 52-53 0.005139849905562021 54-55 0.007060530659745724 56-57 0.02139800671210294 58-59 0.00903531509714587 60-61 0.018530511501631496 62-63 0.013066039496770822 64-65 0.010171492444691156 66-67 0.016393416014582024 68-69 0.027403515549128038 70-71 0.018530511501631496 72-73 0.016961504688354667 74-75 0.018719874392889045 76-77 0.025915664260675875 78-79 0.03814309666759184 80-81 0.03243515808825718 82-83 0.032651572821122944 84-85 0.021695576969793375 86-87 0.024022035348100393 88-89 0.020613503305464527 90-91 0.018855133600930152 92-93 0.031244877057495443 94-95 0.023480998515935967 96-97 0.038061941142767175 98-99 0.0548340829398643 100-101 0.06495147170133901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1848303.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.63711670684812 #Duplication Level Percentage of deduplicated Percentage of total 1 66.33890642736611 27.621607891209198 2 15.590189436969935 12.98261074137976 3 6.701463325077602 8.370888317187557 4 3.4886102484718546 5.810226882413161 5 2.0003713856421474 4.164484842051079 6 1.3256862288591587 3.3118651336602087 7 0.8648597796383205 2.5207187305901786 8 0.6179722448770159 2.0584465985229787 9 0.4460462783817744 1.6714872854673486 >10 2.375033946014407 17.840091258848883 >50 0.17415065404410912 4.982659402933662 >100 0.07308222092483109 5.325690010938484 >500 0.0020730421330533697 0.5744296340800017 >1k 0.0011660861998425206 0.8313261327172075 >5k 1.295651333158356E-4 0.43929193372590664 >10k+ 2.591302666316712E-4 1.4941752042743994 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16496 0.8924943583384326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11201 0.6060153557073705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8143 0.4405662924314898 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2452 0.13266223124671658 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2353 0.12730596660828877 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2137 0.11561957103353726 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2096 0.11340132002166312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.4103683216442324E-5 0.0 0.0 0.0 0.0 3 5.4103683216442324E-5 0.0 0.0 0.0 0.0 4 5.4103683216442324E-5 0.0 0.0 0.0 0.0 5 5.4103683216442324E-5 0.0 0.0 5.4103683216442324E-5 0.0 6 1.0820736643288465E-4 0.0 0.0 5.4103683216442324E-5 5.4103683216442324E-5 7 1.6231104964932697E-4 0.0 0.0 1.0820736643288465E-4 5.4103683216442324E-5 8 1.6231104964932697E-4 0.0 0.0 1.0820736643288465E-4 5.4103683216442324E-5 9 1.6231104964932697E-4 0.0 0.0 2.164147328657693E-4 5.4103683216442324E-5 10-11 2.164147328657693E-4 0.0 0.0 2.164147328657693E-4 5.4103683216442324E-5 12-13 2.7051841608221163E-4 0.0 0.0 3.2462209929865393E-4 1.0820736643288465E-4 14-15 3.787257825150963E-4 0.0 0.0 3.7872578251509624E-4 1.3525920804110581E-4 16-17 3.787257825150963E-4 0.0 0.0 4.8693314894798095E-4 1.6231104964932697E-4 18-19 4.5988130733975975E-4 0.0 0.0 6.221923569890867E-4 1.6231104964932697E-4 20-21 4.8693314894798095E-4 0.0 0.0 7.033478818137503E-4 1.6231104964932697E-4 22-23 5.410368321644233E-4 0.0 0.0 0.0010550218227206253 1.6231104964932697E-4 24-25 8.115552482466349E-4 0.0 0.0 0.0017313178629261546 1.6231104964932697E-4 26-27 8.115552482466349E-4 0.0 0.0 0.0028674952104714435 1.6231104964932697E-4 28-29 8.927107730712983E-4 0.0 0.0 0.0049504870143044725 1.8936289125754812E-4 30-31 9.197626146795195E-4 0.0 0.0 0.011442929000277553 2.164147328657693E-4 32-33 9.197626146795195E-4 0.0 0.0 0.022453028534823567 2.164147328657693E-4 34-35 0.001027969981112404 0.0 0.0 0.03427468331761621 2.164147328657693E-4 36-37 0.0011902810307617311 0.0 0.0 0.046610323090965064 2.164147328657693E-4 38-39 0.0011902810307617311 0.0 0.0 0.06349067225449506 2.164147328657693E-4 40-41 0.0011902810307617311 0.0 0.0 0.08537561211554598 2.164147328657693E-4 42-43 0.0012714365555863945 0.0 0.0 0.11361773475452888 2.164147328657693E-4 44-45 0.0014066957636275005 0.0 0.0 0.14708086282389846 2.7051841608221163E-4 46-47 0.001487851288452164 0.0 0.0 0.18722579577049867 3.2462209929865393E-4 48-49 0.0015149031300603852 0.0 0.0 0.23361970412859795 3.5167394090687514E-4 50-51 0.0015149031300603852 0.0 0.0 0.28715529867126766 3.787257825150963E-4 52-53 0.0015149031300603852 0.0 0.0 0.3557046653065001 3.787257825150963E-4 54-55 0.0015149031300603852 0.0 0.0 0.4296644002633767 3.787257825150963E-4 56-57 0.0016231104964932698 0.0 0.0 0.5189895812537231 3.787257825150963E-4 58-59 0.0016772141797097122 0.0 0.0 0.6302267539467283 3.787257825150963E-4 60-61 0.0017042660213179334 0.0 0.0 0.7583983794864803 3.787257825150963E-4 62-63 0.0017313178629261544 0.0 0.0 0.8832967321916374 4.328294657315386E-4 64-65 0.0017854215461425968 0.0 0.0 1.0186911994407843 4.328294657315386E-4 66-67 0.0017854215461425968 0.0 0.0 1.1801906938418647 4.328294657315386E-4 68-69 0.0017854215461425968 0.0 0.0 1.3771822044329312 4.328294657315386E-4 70-71 0.0018124733877508178 0.0 0.0 1.5864281992725218 4.5988130733975975E-4 72-73 0.0018665770709672602 0.0 0.0 1.7997860740365623 5.13984990556202E-4 74-75 0.0018936289125754814 0.0 0.0 2.04877122419863 5.680886737726445E-4 76-77 0.0018936289125754814 0.0 0.0 2.296484937805111 5.951405153808656E-4 78-79 0.0018936289125754814 0.0 0.0 2.5617011929321114 5.951405153808656E-4 80-81 0.0020288881206165874 0.0 0.0 2.8461513074425566 5.951405153808656E-4 82-83 0.002055939962224808 0.0 0.0 3.184894468060702 5.951405153808656E-4 84-85 0.002137095487049472 0.0 0.0 3.545928346164022 5.951405153808656E-4 86-87 0.0022182510118741354 0.0 0.0 3.9382341531664453 5.951405153808656E-4 88-89 0.0022182510118741354 0.0 0.0 4.369494612084707 5.951405153808656E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13540 0.0 37.837433 1 GGTATCA 8160 0.0 36.10964 1 TATCAAC 16620 0.0 30.683298 2 ATCAACG 16545 0.0 30.67876 3 TCAACGC 16675 0.0 30.439585 4 CAACGCA 16820 0.0 30.177994 5 AACGCAG 17095 0.0 29.689314 6 CGCAGAG 18800 0.0 26.66958 8 ACGCAGA 18955 0.0 26.651358 7 GTACATG 10175 0.0 25.875988 1 GCAGAGT 19455 0.0 24.989985 9 GAGTACT 9985 0.0 24.652565 12-13 TACATGG 10575 0.0 24.133886 2 ACATGGG 10480 0.0 23.21892 3 GTACTTT 10795 0.0 22.757511 14-15 AGTACTT 10320 0.0 22.51695 12-13 CATGGGA 6140 0.0 21.208773 4 AGAGTAC 16645 0.0 20.999489 10-11 ACTTTTT 12470 0.0 20.117928 16-17 ATGGGAT 2630 0.0 20.05914 5 >>END_MODULE