FastQCFastQC Report
Thu 26 May 2016
SRR936802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848303
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149320.8078761977879169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110250.5964931074612766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85490.46253238781736544No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32710.17697314780098283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32650.1766485257016842No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.12979473603624514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.1240056419320858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.035.0415731
GTATCAA143400.033.9303551
TATCAAC179050.027.1915742
ATCAACG179250.027.1869983
TCAACGC179650.027.123534
CAACGCA182250.026.7626445
AACGCAG186350.026.1483336
GAGTACT109050.024.36724312-13
GTACATG100750.024.0998021
CGCAGAG203850.023.6472918
ACGCAGA205100.023.5494827
AGTACTT111800.023.13066112-13
GCAGAGT208250.022.9196059
GTACTTT116150.022.79687114-15
TACATGG103150.022.4256252
ACATGGG102900.021.3716813
AGAGTAC179850.020.4124510-11
CATGGGA60350.020.06734
ACTTTTT133600.020.03312916-17
CGTATAG2151.0760941E-619.8903141
Produced by FastQC (version 0.11.5)