##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1848303 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89945750236839 31.0 31.0 34.0 30.0 34.0 2 31.98219718303763 31.0 31.0 34.0 30.0 34.0 3 32.10379899832441 33.0 31.0 34.0 30.0 34.0 4 35.86816663718016 37.0 35.0 37.0 35.0 37.0 5 35.76133945570613 37.0 35.0 37.0 35.0 37.0 6 35.79236034351511 37.0 35.0 37.0 35.0 37.0 7 35.73397327169842 37.0 35.0 37.0 33.0 37.0 8 35.71734071740402 37.0 35.0 37.0 33.0 37.0 9 37.338585718899985 39.0 37.0 39.0 34.0 39.0 10-11 37.2249636558508 39.0 37.0 39.0 33.0 39.0 12-13 37.13375512564769 39.0 37.0 39.0 33.0 39.0 14-15 38.22683672536375 40.0 37.5 41.0 33.0 41.0 16-17 38.14521482678977 40.0 37.0 41.0 33.0 41.0 18-19 38.19576633268463 40.0 37.5 41.0 33.5 41.0 20-21 38.29616816073988 40.0 38.0 41.0 34.0 41.0 22-23 38.2078685150649 40.0 37.5 41.0 33.0 41.0 24-25 38.10547296628313 40.0 37.0 41.0 33.0 41.0 26-27 37.89090614471762 40.0 37.0 41.0 33.0 41.0 28-29 37.69855456599919 39.5 37.0 41.0 32.5 41.0 30-31 37.59552681567903 39.0 37.0 41.0 33.0 41.0 32-33 37.46058384366633 39.0 36.0 41.0 32.5 41.0 34-35 37.30767195638377 39.0 36.0 41.0 32.0 41.0 36-37 37.102175346791086 39.0 35.5 41.0 31.5 41.0 38-39 36.92000256451458 39.0 35.0 41.0 31.0 41.0 40-41 36.653445079080655 39.0 35.0 41.0 30.5 41.0 42-43 36.36627003256501 39.0 35.0 40.0 30.0 41.0 44-45 36.043678173979046 39.0 35.0 40.0 30.0 41.0 46-47 35.59121123538727 38.0 35.0 40.0 28.0 41.0 48-49 35.1413399209978 38.0 34.5 40.0 26.5 41.0 50-51 34.864184065058595 38.0 34.0 40.0 26.0 41.0 52-53 34.60589849175162 38.0 34.0 40.0 25.0 41.0 54-55 34.29031197806853 38.0 33.5 40.0 24.0 41.0 56-57 34.0048631095659 37.5 33.0 40.0 23.5 41.0 58-59 33.6133060975392 37.0 33.0 40.0 22.5 41.0 60-61 33.22432793757301 36.5 32.5 39.0 22.0 41.0 62-63 32.84921087072844 36.0 32.0 39.0 21.0 40.0 64-65 32.3885577743476 35.5 31.0 39.0 19.5 40.0 66-67 31.92165867825784 35.0 31.0 38.0 18.0 40.0 68-69 31.450713167700314 35.0 30.5 38.0 16.0 40.0 70-71 31.02656328534878 35.0 30.0 37.0 13.0 39.0 72-73 30.61158965818916 34.0 30.0 36.5 9.5 39.0 74-75 30.10954724414774 34.0 29.0 36.0 7.5 39.0 76-77 28.71864380461429 32.5 27.5 34.5 7.0 36.5 78-79 29.437195091930274 34.0 29.0 35.0 4.0 37.0 80-81 29.163201325756653 34.0 29.0 35.0 2.0 37.0 82-83 28.94343892749187 34.0 29.0 35.0 2.0 36.0 84-85 28.576208013512936 34.0 29.0 35.0 2.0 36.0 86-87 28.169991067481902 33.0 27.5 35.0 2.0 35.5 88-89 27.74235690793122 33.0 27.0 35.0 2.0 35.0 90-91 27.227525465251098 33.0 26.0 35.0 2.0 35.0 92-93 26.708156617178027 32.5 25.0 34.5 2.0 35.0 94-95 26.116250419979842 32.0 24.5 34.0 2.0 35.0 96-97 25.34195286162496 32.0 21.5 34.0 2.0 35.0 98-99 24.385115698021373 31.5 10.0 34.0 2.0 35.0 100-101 21.148414518615184 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2728551291880912 1101 2 0.15685471349097568 1101 3 0.23231063657229356 1101 4 0.19722969027875337 1101 5 0.2825947226189953 1101 6 0.27984582775810196 1101 7 0.19179077722584026 1101 8 0.2455299606906607 1101 9 0.37683279128972913 1101 10-11 0.26573385057819365 1101 12-13 0.19936060072198103 1101 14-15 0.3453005750528533 1101 16-17 0.3123928598043122 1101 18-19 0.2994910523773697 1101 20-21 0.26601765294273605 1101 22-23 0.3012361918608022 1101 24-25 0.27966174119752907 1101 26-27 0.17185103706217575 1101 28-29 0.21590374031681847 1101 30-31 0.3117043563356603 1101 32-33 0.46105775277784034 1101 34-35 0.4776698278836591 1101 36-37 0.5834520716997744 1101 38-39 0.4053562602470109 1101 40-41 0.46009891086448107 1101 42-43 0.5498400308885323 1101 44-45 0.5563494195932037 1101 46-47 0.638259079185211 1101 48-49 0.6863915966786891 1101 50-51 0.6172545506231231 1101 52-53 0.7456393385527633 1101 54-55 0.802043464193396 1101 56-57 0.7735676710438639 1101 58-59 0.8299033961014928 1101 60-61 0.5632538122726629 1101 62-63 0.5365498370043369 1101 64-65 0.6211563096055812 1101 66-67 0.6410177478023193 1101 68-69 0.6433297100063236 1101 70-71 0.5651330410596245 1101 72-73 0.4700055341719711 1101 74-75 0.09478820675099442 1101 76-77 0.14920536137349316 1101 78-79 0.5987105581628285 1101 80-81 0.837081621411965 1101 82-83 0.8999793378292189 1101 84-85 0.6517175335679859 1101 86-87 0.5487378040729318 1101 88-89 0.5257255095436761 1101 90-91 0.3641022205965747 1101 92-93 0.6045424029948911 1101 94-95 0.7106758958652435 1101 96-97 1.0481597140281842 1101 98-99 1.1285512959648543 1101 100-101 1.3163626621522333 1102 1 0.33341121373510063 1102 2 0.2629454212286326 1102 3 0.23429079962424026 1102 4 0.21301067710233923 1102 5 0.19055225982791058 1102 6 0.20495518811583224 1102 7 0.18918104930778412 1102 8 0.20988404955870976 1102 9 0.2968127753730272 1102 10-11 0.3210145267860156 1102 12-13 0.3030340412790977 1102 14-15 0.42002881354702737 1102 16-17 0.414783261846857 1102 18-19 0.3918711151116341 1102 20-21 0.34517288522597767 1102 22-23 0.2760022082152034 1102 24-25 0.24045570324783938 1102 26-27 0.3787046631316002 1102 28-29 0.33359496940482103 1102 30-31 0.5219561049009869 1102 32-33 0.43696087478862466 1102 34-35 0.44889079681831134 1102 36-37 0.48368856031115826 1102 38-39 0.49189692531592044 1102 40-41 0.5413027587300192 1102 42-43 0.31453941812925734 1102 44-45 0.42714685585745116 1102 46-47 0.6326699796851969 1102 48-49 0.7170678541070217 1102 50-51 0.5985036168413203 1102 52-53 0.6485362496372886 1102 54-55 0.6594854149595619 1102 56-57 0.7359661008817042 1102 58-59 0.6076306887602669 1102 60-61 0.6927136186333485 1102 62-63 0.47321140511490256 1102 64-65 0.4991519758659706 1102 66-67 0.3748059564670605 1102 68-69 0.3547793778528181 1102 70-71 0.34766580055572405 1102 72-73 0.29390802681630035 1102 74-75 0.4033305468235646 1102 76-77 0.28178223802937197 1102 78-79 0.4074401010249247 1102 80-81 0.4798289567732148 1102 82-83 0.3946818064258757 1102 84-85 0.3231156259050927 1102 86-87 0.4131707423194193 1102 88-89 0.33768561501216254 1102 90-91 0.4191114676405725 1102 92-93 0.5027275974467074 1102 94-95 0.6620957303132897 1102 96-97 0.5596190644354948 1102 98-99 1.0294368931358022 1102 100-101 1.1957556528909024 1103 1 0.19788033062488708 1103 2 0.1761655328086995 1103 3 0.15568589010903366 1103 4 0.21425226505599682 1103 5 0.17979242666778106 1103 6 0.22669270824049903 1103 7 0.22612802186475278 1103 8 0.13684563242050274 1103 9 0.14990126512585533 1103 10-11 0.2151872254743452 1103 12-13 0.2807665823421601 1103 14-15 0.4041060239538936 1103 16-17 0.3494230494185473 1103 18-19 0.41300263625348776 1103 20-21 0.36662274697718544 1103 22-23 0.36224063957249086 1103 24-25 0.308588338681119 1103 26-27 0.30755156512935855 1103 28-29 0.24440746877181851 1103 30-31 0.3799671572579868 1103 32-33 0.3880613343866699 1103 34-35 0.5214622283402264 1103 36-37 0.5685934563023594 1103 38-39 0.45655555714239426 1103 40-41 0.47713885561598346 1103 42-43 0.5222108906203999 1103 44-45 0.49423758610856083 1103 46-47 0.4468256909401376 1103 48-49 0.6830349988936177 1103 50-51 0.5031663634634427 1103 52-53 0.5422478211297843 1103 54-55 0.6075790154395193 1103 56-57 0.5864227697202864 1103 58-59 0.6201236214200065 1103 60-61 0.6557576102075586 1103 62-63 0.7489306155904742 1103 64-65 0.624667839574883 1103 66-67 0.4709047052077544 1103 68-69 0.5273281303369366 1103 70-71 0.5327249616656644 1103 72-73 0.5459923833045615 1103 74-75 0.5812634855840066 1103 76-77 0.5102223074804542 1103 78-79 0.7283149240559474 1103 80-81 1.0279381068911668 1103 82-83 0.9165509920677017 1103 84-85 0.8387003151317707 1103 86-87 0.7699165744504555 1103 88-89 0.5060025027332493 1103 90-91 0.647754000130476 1103 92-93 0.7133700834664332 1103 94-95 0.9614473123946254 1103 96-97 1.23704400863652 1103 98-99 1.3812413958581793 1103 100-101 1.4715173617385275 1104 1 0.29664992493299636 1104 2 0.22625278695632645 1104 3 0.22764536233001564 1104 4 0.22706859542874014 1104 5 0.11468077388090592 1104 6 0.1534355843367763 1104 7 0.19837071908953163 1104 8 0.22806545530236377 1104 9 0.2438026456179756 1104 10-11 0.24587555656782456 1104 12-13 0.2555591328034197 1104 14-15 0.32637660735780827 1104 16-17 0.2872572719920754 1104 18-19 0.33520292345789926 1104 20-21 0.34676629262456515 1104 22-23 0.3695011461778108 1104 24-25 0.2325376478492558 1104 26-27 0.4390480803164678 1104 28-29 0.33561315161354344 1104 30-31 0.451853707272015 1104 32-33 0.40262628272199663 1104 34-35 0.4591550505124573 1104 36-37 0.4878337281062457 1104 38-39 0.4988613452062154 1104 40-41 0.5373870416003328 1104 42-43 0.6860880977039159 1104 44-45 0.6879471468562954 1104 46-47 0.7188852140450592 1104 48-49 0.6820476200914314 1104 50-51 0.6555290328686993 1104 52-53 0.5999869579934511 1104 54-55 0.5511529833024369 1104 56-57 0.5147060011881024 1104 58-59 0.7406102973611937 1104 60-61 0.7876564095363392 1104 62-63 0.7682468194098675 1104 64-65 0.7841110330746659 1104 66-67 0.6104902323514771 1104 68-69 0.6246031224033608 1104 70-71 0.6749571003204728 1104 72-73 0.7197902425428886 1104 74-75 0.5910320313071402 1104 76-77 0.74985326455311 1104 78-79 1.0094762899919019 1104 80-81 1.0240664629034413 1104 82-83 0.9105623820720261 1104 84-85 0.7742418311985944 1104 86-87 0.8226193320208814 1104 88-89 0.883129594019934 1104 90-91 1.019717855763897 1104 92-93 1.0219928213377578 1104 94-95 1.2622726261368697 1104 96-97 1.3334937273608212 1104 98-99 1.4466628165241708 1104 100-101 1.16907563940892 1105 1 0.17082833108940676 1105 2 0.1449263146279911 1105 3 0.10634933885380349 1105 4 0.12117617157270644 1105 5 0.15145119290202302 1105 6 0.13778634011470103 1105 7 0.12952418560782775 1105 8 0.08877197008268922 1105 9 0.06655291496419125 1105 10-11 0.06757485902664229 1105 12-13 0.17729821136558854 1105 14-15 0.20895902026954616 1105 16-17 0.21048598373420901 1105 18-19 0.20948604281625194 1105 20-21 0.2854245958701682 1105 22-23 0.07377274175773607 1105 24-25 0.15322096593899204 1105 26-27 0.19715174233847677 1105 28-29 0.08082952658523368 1105 30-31 0.17216133612411255 1105 32-33 0.06653333574735854 1105 34-35 0.1737341322021706 1105 36-37 0.23145554364781873 1105 38-39 0.39141793028181127 1105 40-41 0.480600102819956 1105 42-43 0.47766802711251444 1105 44-45 0.42989674689021484 1105 46-47 0.37001161065605714 1105 48-49 0.4735137817485935 1105 50-51 0.3724637887676323 1105 52-53 0.3296927831366787 1105 54-55 0.2315572124801193 1105 56-57 0.13945851904527018 1105 58-59 0.34576195254349784 1105 60-61 0.42790669432855566 1105 62-63 0.5498229629515308 1105 64-65 0.3250517645501745 1105 66-67 0.42883967106527265 1105 68-69 0.40087969138102864 1105 70-71 0.4772897866263506 1105 72-73 0.36721077182711426 1105 74-75 0.3097654468344757 1105 76-77 0.2694786277737009 1105 78-79 0.3698435254760213 1105 80-81 0.46924169826314355 1105 82-83 0.7079362644476745 1105 84-85 0.3052057274074649 1105 86-87 0.1962331603845442 1105 88-89 0.3349656755688315 1105 90-91 0.47853542107862523 1105 92-93 0.7143508667737031 1105 94-95 0.7635112885093349 1105 96-97 0.7575024086498416 1105 98-99 1.1131328396803823 1105 100-101 1.3299330136364347 1106 1 0.13593557460428585 1106 2 0.11846637773866675 1106 3 0.12550217937841524 1106 4 0.1734948749456322 1106 5 0.03615728009105368 1106 6 0.17791241045674866 1106 7 0.08822042532802499 1106 8 0.09368970834059809 1106 9 0.2517937928639711 1106 10-11 0.16671245598396212 1106 12-13 0.13982244306337321 1106 14-15 0.17491516436655985 1106 16-17 0.1553127758138615 1106 18-19 0.2044246188352119 1106 20-21 0.23333208091918323 1106 22-23 0.32506241550675696 1106 24-25 0.26157898900998333 1106 26-27 0.3393501960846663 1106 28-29 0.27795967631374907 1106 30-31 0.10271960290564408 1106 32-33 0.29784847318263985 1106 34-35 0.4434676562577664 1106 36-37 0.3798035020557933 1106 38-39 0.5014014674956329 1106 40-41 0.5733182569447663 1106 42-43 0.38956191820406616 1106 44-45 0.3941911778136955 1106 46-47 0.40005069894682066 1106 48-49 0.5149336898385926 1106 50-51 0.26775576059990414 1106 52-53 0.5724241621220969 1106 54-55 0.6904163478385072 1106 56-57 0.5605189818643126 1106 58-59 0.5399426585489593 1106 60-61 0.43748733410978247 1106 62-63 0.41787649665427296 1106 64-65 0.3465952956612455 1106 66-67 0.2625500961418332 1106 68-69 0.2981855951764416 1106 70-71 0.5303621182858684 1106 72-73 0.23731110532456157 1106 74-75 0.45287653810644457 1106 76-77 0.22975396692863725 1106 78-79 0.4405136938992307 1106 80-81 0.8066367627140707 1106 82-83 0.729515772509508 1106 84-85 0.5074725862176983 1106 86-87 0.6728832310170709 1106 88-89 0.5450466392536519 1106 90-91 0.5815698547958696 1106 92-93 0.6433818897871824 1106 94-95 0.7976227677549659 1106 96-97 0.8034501936578025 1106 98-99 0.8207461620784997 1106 100-101 0.8148090363978504 1107 1 0.055575651475553656 1107 2 -0.008357009327738751 1107 3 0.06490190613952507 1107 4 0.08136682922152261 1107 5 0.08553622419117346 1107 6 0.0487473945050354 1107 7 0.07495555429348855 1107 8 0.10023882831880826 1107 9 0.14158300567756044 1107 10-11 0.09437912257546799 1107 12-13 0.08418040269625493 1107 14-15 0.05620731960539871 1107 16-17 0.15214396680666908 1107 18-19 0.28317031748731125 1107 20-21 0.19924869728461658 1107 22-23 0.028149171673177875 1107 24-25 0.12655494024458136 1107 26-27 0.2190775073693274 1107 28-29 0.20906460270858673 1107 30-31 0.20054658933862157 1107 32-33 0.2731812110936005 1107 34-35 0.28967077886554193 1107 36-37 0.27521645738536193 1107 38-39 0.2564630274195707 1107 40-41 0.3355784372806312 1107 42-43 0.2805824873337528 1107 44-45 0.2732891692891286 1107 46-47 0.43182695241727487 1107 48-49 0.5020195558027112 1107 50-51 0.49368078292823725 1107 52-53 0.5026944674944005 1107 54-55 0.5355174279641517 1107 56-57 0.46277982060713896 1107 58-59 0.48701124809467444 1107 60-61 0.5744192974455302 1107 62-63 0.60389551785115 1107 64-65 0.6611979278911235 1107 66-67 0.5980710166849654 1107 68-69 0.7071568861524149 1107 70-71 0.5161787469851546 1107 72-73 0.6299833289159587 1107 74-75 0.7730504883250156 1107 76-77 0.6789411838220971 1107 78-79 0.7082713469219151 1107 80-81 0.6606559877465266 1107 82-83 0.6883035550918031 1107 84-85 0.7843341811815172 1107 86-87 0.7635622177107102 1107 88-89 0.6374883302141079 1107 90-91 0.4209128052302269 1107 92-93 0.7972490047718708 1107 94-95 0.7334975369461176 1107 96-97 0.6836611634844978 1107 98-99 0.7243299027635643 1107 100-101 0.8712117397447017 1108 1 0.05765751820980114 1108 2 0.0047621301423141915 1108 3 0.06306994788949538 1108 4 0.004048429386720898 1108 5 0.0635313715081196 1108 6 0.10170804741147776 1108 7 0.010582648596987099 1108 8 0.07561779752257536 1108 9 0.04631343303280033 1108 10-11 0.09919285275878309 1108 12-13 0.05583878601128589 1108 14-15 0.08978833219233451 1108 16-17 0.1889787174613602 1108 18-19 0.10663742812284482 1108 20-21 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-1.0774157720473347 2316 92-93 -1.1088822871064714 2316 94-95 -1.254543673133007 2316 96-97 -1.4637558467438048 2316 98-99 -1.605454633684893 2316 100-101 -1.8139621067726175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 20.0 5 50.0 6 150.0 7 349.0 8 764.0 9 1468.0 10 2367.0 11 3945.0 12 5862.0 13 7987.0 14 9952.0 15 13510.0 16 18328.0 17 24255.0 18 21098.0 19 13471.0 20 12403.0 21 12955.0 22 13810.0 23 15231.0 24 17665.0 25 20781.0 26 25402.0 27 31162.0 28 37992.0 29 47061.0 30 56802.0 31 70152.0 32 87117.0 33 111283.0 34 147396.0 35 201813.0 36 281524.0 37 348390.0 38 178336.0 39 7442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.574919161717155 20.10791065171215 15.466074980044105 21.85109520652659 2 16.00125312876385 21.85941366992502 38.70560888747488 23.43372431383625 3 18.988555452568516 26.135675594073792 30.564749538587403 24.31101941477029 4 13.484369175400355 17.832141158673657 36.67667043769339 32.0068192282326 5 13.596952315819033 37.99812035668856 33.471177449907294 14.933749877585118 6 30.725860424400114 37.12767874098565 17.19810009506017 14.948360739554067 7 27.547864175949506 33.903910776533934 21.007053497180927 17.541171550335633 8 26.66857111631589 33.9923161949096 19.913239333594113 19.4258733551804 9 27.076704497806364 16.297399611859756 20.56575200684304 36.06014388349084 10-11 24.717808714263843 27.07740559854093 27.003932796733004 21.200852890462222 12-13 26.23599931180078 24.80014088606732 27.741462163650745 21.22239763848116 14-15 23.36567651516012 25.38512354305544 26.86329027221186 24.385909669572577 16-17 21.51181003527023 28.849332440982284 27.22535452829382 22.413502995453662 18-19 21.56044734934153 26.77151529579812 30.696318620077694 20.971718734782655 20-21 22.53610513108653 25.889136025164976 30.689591366690056 20.885167477058435 22-23 22.33432505384669 25.824743021030645 30.622062508149366 21.2188694169733 24-25 22.060597108440774 26.052087239145834 30.783077869524355 21.104237782889037 26-27 21.90605660951608 26.23175408339251 30.71068276351831 21.151506543573095 28-29 21.685220871647683 26.198723205205653 30.880583677620272 21.23547224552639 30-31 21.768068887825105 26.156093022702017 30.75768002318559 21.318158066287292 32-33 21.759804056828713 26.31782549392926 30.522736949971623 21.399633499270408 34-35 22.070758293851682 26.134124075496363 30.416808807818757 21.378308822833194 36-37 22.312968459865353 26.024689794782006 30.26684642755006 21.395495317802578 38-39 22.067960227351122 26.26163018297442 30.301687440092266 21.368722149582194 40-41 21.972758232969973 26.23633880520616 30.283753526560535 21.507149435263333 42-43 21.870540382240392 26.48142161285479 29.82081044485596 21.827227560048858 44-45 22.113954993247688 26.48050378302573 29.604854522317254 21.800686701409326 46-47 22.40705242136849 26.701250302309237 29.12862439693703 21.76307287938524 48-49 22.457700939474144 26.965245299924035 28.604482683247234 21.972571077354583 50-51 22.35856496103731 27.12563421737137 28.468720960588236 22.047079861003088 52-53 22.49331664726993 27.310389455100477 28.16898588880611 22.02730800882349 54-55 22.327762443606 27.24596020545716 28.122239815264937 22.3040375356719 56-57 22.36232153793881 27.52895646358909 27.982402131264973 22.12631986720713 58-59 22.556080997183066 27.057682886284134 28.097564960843464 22.28867115568934 60-61 22.4370138445915 27.091202037761125 28.453397521943103 22.018386595704275 62-63 22.423905833570487 27.022099202403073 28.63154938965602 21.92244557437042 64-65 22.30139006797487 27.144471083220655 28.455407283802526 22.09873156500195 66-67 22.232523009725995 27.30709310664507 28.13220512207335 22.328178761555588 68-69 22.11410692296206 27.242241993113613 28.448063176438254 22.19558790748608 70-71 22.204427467286354 27.18618032880018 28.259258157106643 22.35013404680682 72-73 22.294382542282776 27.257635789835362 27.958482189357035 22.489499478524827 74-75 22.27626238087148 27.648448718600765 27.68083133230243 22.394457568225324 76-77 22.323546900590237 27.423428463526633 27.877157661969044 22.37586697391409 78-79 22.125111498471824 27.467271461612736 27.821983372828434 22.585633667087006 80-81 22.301246876856435 27.69520932230625 27.52505277828575 22.47849102255156 82-83 22.138370020324903 27.5133905467704 27.700001974867245 22.648237458037453 84-85 22.036338492702438 27.497879118405788 27.4877345954743 22.978047793417474 86-87 22.140850282664694 27.482777445040124 27.612950906858885 22.763421365436294 88-89 22.16831004718728 27.33821590798115 27.517761846393405 22.975712198438163 90-91 21.992860439335104 27.37945228380998 27.632875307107696 22.994811969747218 92-93 21.88838392035925 27.613969593680682 27.478737217984094 23.018909267975978 94-95 21.885551123536995 27.449091526368257 27.35338156704093 23.311975783053818 96-97 21.99244601514043 27.475009414076535 27.092305561446867 23.440239009336167 98-99 21.773368684708636 27.625633588459458 27.04631793027274 23.554679796559167 100-101 21.667707267033563 27.80878237854192 27.01204679780035 23.51146355662417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3399.0 1 2268.0 2 1276.0 3 1362.5 4 1154.5 5 1169.0 6 1938.5 7 3934.5 8 6026.5 9 6513.0 10 5420.0 11 4332.0 12 4131.0 13 4141.0 14 4133.5 15 4061.5 16 4105.5 17 4293.5 18 4370.5 19 4444.0 20 4737.5 21 5264.5 22 5990.0 23 6788.5 24 7602.0 25 8928.0 26 10999.0 27 13089.5 28 14626.5 29 16533.5 30 19294.5 31 22528.0 32 26067.0 33 30041.0 34 34069.0 35 37560.0 36 42172.5 37 46426.0 38 48319.0 39 48835.5 40 49369.0 41 50966.5 42 52885.0 43 55343.5 44 58844.5 45 61942.0 46 62773.5 47 64733.5 48 70427.5 49 70569.0 50 66164.5 51 63893.5 52 62964.0 53 63329.5 54 62502.0 55 59328.0 56 54967.0 57 50363.5 58 46882.5 59 42969.5 60 36623.5 61 29664.0 62 23711.5 63 18327.0 64 13547.5 65 9946.0 66 7139.5 67 5522.0 68 4082.0 69 2757.5 70 1856.5 71 1112.5 72 696.0 73 516.0 74 365.5 75 239.0 76 158.5 77 79.0 78 38.0 79 21.5 80 10.5 81 7.5 82 5.0 83 3.5 84 2.0 85 1.5 86 2.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02586156057745943 2 0.006979375134921061 3 0.009414040879660964 4 0.0 5 0.004328294657315386 6 0.0 7 0.0 8 0.0 9 1.0820736643288465E-4 10-11 0.0 12-13 5.4103683216442324E-5 14-15 0.0 16-17 1.0820736643288465E-4 18-19 0.004490605706964713 20-21 0.0010820736643288465 22-23 0.0 24-25 0.005193953588778463 26-27 0.006248975411499089 28-29 0.008629537473022551 30-31 0.032651572821122944 32-33 6.492441985973079E-4 34-35 0.004409450182140049 36-37 0.015825327340809377 38-39 0.008034396957641684 40-41 0.01000918139504183 42-43 0.0065194938275813 44-45 0.002542873111172789 46-47 0.0016231104964932698 48-49 0.0018936289125754814 50-51 0.0032732728345947603 52-53 0.0029215988936878855 54-55 0.0018665770709672604 56-57 5.951405153808656E-4 58-59 8.927107730712984E-4 60-61 0.0 62-63 5.4103683216442324E-5 64-65 0.006492441985973079 66-67 4.5988130733975975E-4 68-69 0.0010279699811124043 70-71 0.003679050458718078 72-73 0.00976571482056784 74-75 0.004815227806263367 76-77 0.0034626357258523087 78-79 0.009928025870217166 80-81 2.7051841608221163E-4 82-83 0.0041930354492742804 84-85 8.115552482466349E-4 86-87 0.0 88-89 0.0015960586548850486 90-91 5.410368321644233E-4 92-93 1.6231104964932697E-4 94-95 5.680886737726444E-4 96-97 3.787257825150963E-4 98-99 8.115552482466348E-5 100-101 5.951405153808656E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1848303.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.140170125766886 #Duplication Level Percentage of deduplicated Percentage of total 1 65.81197895917322 27.07516010693777 2 15.92467019491281 13.102872820308844 3 6.785455687891001 8.37464404142065 4 3.4566780758554834 5.688332964428125 5 2.0632819058807845 4.244188431267601 6 1.2580461305721298 3.1053739102680096 7 0.8966126488154522 2.5820757836427526 8 0.6231709653836695 2.050988762665804 9 0.4883307977665923 1.8081010887992162 >10 2.433829575274129 17.94706474292212 >50 0.17587477546468164 5.000884150968113 >100 0.07851952208739454 5.644916552010492 >500 0.0018411352933085474 0.4739990062390666 >1k 0.001315096638077534 1.034509188254445 >5k 1.315096638077534E-4 0.46252910676153186 >10k+ 2.630193276155068E-4 1.404359343105519 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14932 0.8078761977879169 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11025 0.5964931074612766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8549 0.46253238781736544 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3271 0.17697314780098283 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3265 0.1766485257016842 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2399 0.12979473603624514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2292 0.1240056419320858 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.4103683216442324E-5 0.0 5 0.0 0.0 0.0 5.4103683216442324E-5 0.0 6 0.0 0.0 0.0 5.4103683216442324E-5 0.0 7 5.4103683216442324E-5 0.0 0.0 1.0820736643288465E-4 0.0 8 5.4103683216442324E-5 0.0 0.0 1.0820736643288465E-4 0.0 9 5.4103683216442324E-5 0.0 0.0 1.6231104964932697E-4 0.0 10-11 5.4103683216442324E-5 0.0 0.0 1.6231104964932697E-4 0.0 12-13 1.6231104964932697E-4 0.0 0.0 1.6231104964932697E-4 0.0 14-15 2.164147328657693E-4 0.0 0.0 2.164147328657693E-4 2.7051841608221162E-5 16-17 2.4346657447399048E-4 0.0 0.0 2.975702576904328E-4 5.4103683216442324E-5 18-19 3.787257825150963E-4 0.0 0.0 3.7872578251509624E-4 5.4103683216442324E-5 20-21 4.8693314894798095E-4 0.0 0.0 6.492441985973079E-4 5.4103683216442324E-5 22-23 4.8693314894798095E-4 0.0 0.0 0.0010820736643288465 5.4103683216442324E-5 24-25 5.410368321644233E-4 0.0 0.0 0.0015960586548850486 5.4103683216442324E-5 26-27 5.680886737726445E-4 0.0 0.0 0.0027592878440385587 5.4103683216442324E-5 28-29 8.656589314630772E-4 0.0 0.0 0.004815227806263367 5.4103683216442324E-5 30-31 9.197626146795195E-4 0.0 0.0 0.011280617950628225 5.4103683216442324E-5 32-33 9.197626146795195E-4 0.0 0.0 0.021966095385875586 5.4103683216442324E-5 34-35 9.468144562877407E-4 0.0 0.0 0.03367954280223535 5.4103683216442324E-5 36-37 9.738662978959619E-4 0.0 0.0 0.04585287152593487 5.4103683216442324E-5 38-39 0.0012443847139781735 0.0 0.0 0.06294963542233065 5.4103683216442324E-5 40-41 0.0012443847139781735 0.0 0.0 0.08494278264981445 5.4103683216442324E-5 42-43 0.0014066957636275005 0.0 0.0 0.11334721633844666 5.4103683216442324E-5 44-45 0.0015149031300603852 0.0 0.0 0.14681034440781626 8.115552482466348E-5 46-47 0.0017313178629261544 0.0 0.0 0.18706348472084933 1.0820736643288465E-4 48-49 0.0017313178629261544 0.0 0.0 0.23337623755412396 1.0820736643288465E-4 50-51 0.0017313178629261544 0.0 0.0 0.28696593578001006 1.0820736643288465E-4 52-53 0.0017313178629261544 0.0 0.0 0.35567761346489185 1.0820736643288465E-4 54-55 0.0017854215461425968 0.0 0.0 0.42952914105533563 1.0820736643288465E-4 56-57 0.001839525229359039 0.0 0.0 0.5182862333719093 1.0820736643288465E-4 58-59 0.001839525229359039 0.0 0.0 0.628955317391142 1.0820736643288465E-4 60-61 0.001839525229359039 0.0 0.0 0.7561530766329979 1.0820736643288465E-4 62-63 0.001839525229359039 0.0 0.0 0.8787790746430645 2.164147328657693E-4 64-65 0.001839525229359039 0.0 0.0 1.0121176019299867 2.164147328657693E-4 66-67 0.0018665770709672602 0.0 0.0 1.1723997634586971 2.164147328657693E-4 68-69 0.0020018362790083662 0.0 0.0 1.3660909493735605 2.7051841608221163E-4 70-71 0.002055939962224808 0.0 0.0 1.570575820090104 2.7051841608221163E-4 72-73 0.0020829918038330294 0.0 0.0 1.7786585857405415 2.7051841608221163E-4 74-75 0.0021100436454412506 0.0 0.0 2.0227744044131293 2.7051841608221163E-4 76-77 0.0021100436454412506 0.0 0.0 2.265591734688522 2.7051841608221163E-4 78-79 0.0021100436454412506 0.0 0.0 2.5264796951582076 3.787257825150963E-4 80-81 0.002488769427956347 0.0 0.0 2.80641215212008 3.787257825150963E-4 82-83 0.002488769427956347 0.0 0.0 3.140718810714477 4.8693314894798095E-4 84-85 0.002651080477605674 0.0 0.0 3.4972079794276154 4.8693314894798095E-4 86-87 0.0027592878440385587 0.0 0.0 3.8813170784227475 5.13984990556202E-4 88-89 0.0027592878440385587 0.0 0.0 4.30346106671904 5.410368321644233E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9180 0.0 35.041573 1 GTATCAA 14340 0.0 33.930355 1 TATCAAC 17905 0.0 27.191574 2 ATCAACG 17925 0.0 27.186998 3 TCAACGC 17965 0.0 27.12353 4 CAACGCA 18225 0.0 26.762644 5 AACGCAG 18635 0.0 26.148333 6 GAGTACT 10905 0.0 24.367243 12-13 GTACATG 10075 0.0 24.099802 1 CGCAGAG 20385 0.0 23.647291 8 ACGCAGA 20510 0.0 23.549482 7 AGTACTT 11180 0.0 23.130661 12-13 GCAGAGT 20825 0.0 22.919605 9 GTACTTT 11615 0.0 22.796871 14-15 TACATGG 10315 0.0 22.425625 2 ACATGGG 10290 0.0 21.371681 3 AGAGTAC 17985 0.0 20.41245 10-11 CATGGGA 6035 0.0 20.0673 4 ACTTTTT 13360 0.0 20.033129 16-17 CGTATAG 215 1.0760941E-6 19.890314 1 >>END_MODULE