FastQCFastQC Report
Thu 26 May 2016
SRR936801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881928
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165110.877344935619216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105200.5590011945196628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.4196759918551613No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.13130151631730863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22880.12157744610845897No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.11371317074829644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.11121573195148804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134700.036.5525971
GGTATCA81950.034.4479681
ATCAACG166900.029.3596953
TCAACGC167900.029.213144
TATCAAC168500.029.0801352
CAACGCA170750.028.7255425
AACGCAG172850.028.4850166
GTACATG104100.026.9356481
TACATGG105500.026.0383262
CGCAGAG191850.025.4390058
ACGCAGA192500.025.3537797
GTACCGT1151.2775254E-424.795886
ACATGGG107100.024.540583
GCAGAGT197600.024.215839
GAGTACT102100.023.780312-13
AGTACTT106750.023.0337512-13
GTACTTT109200.022.14300214-15
CATGGGG45900.021.744824
AGAGTAC171950.020.9332810-11
CATGGGA63550.020.6415714
Produced by FastQC (version 0.11.5)