FastQCFastQC Report
Thu 26 May 2016
SRR936801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881928
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148680.7900408517222762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108880.5785556089287156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85270.45309916213585216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33230.1765742366339201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30900.16419331664123177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.12593467975395445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.12008961022950931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91300.033.8229641
GTATCAA139800.033.3373341
TATCAAC175650.026.5517942
ATCAACG177750.026.1038133
TCAACGC179150.025.8998244
CAACGCA181700.025.542455
AACGCAG185100.025.0732736
GTACATG101250.024.3054581
TACATGG105050.023.011892
ACGCAGA204150.022.5009067
CATATAG3400.022.3491023
ACATGGG103550.022.2438873
CGCAGAG205600.022.2035888
GAGTACT111150.021.8552212-13
AGTACTT114850.021.85410312-13
CGATTTA1005.3762415E-721.3818422-23
GCAGAGT209900.021.3413649
CATGGGG44050.020.7001914
GTACTTT117500.020.61348214-15
AGAGTAC180350.019.89945610-11
Produced by FastQC (version 0.11.5)