FastQCFastQC Report
Thu 26 May 2016
SRR936800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904923
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167560.8796156065100794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110250.578763551072668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82040.43067357578232823No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.13139638715055674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24440.1282991490994649No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.11328541888569774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21110.11081812755686189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136150.036.0268141
GGTATCA85150.035.1657371
ATCAACG169200.028.991953
TATCAAC169700.028.9065252
TCAACGC170300.028.7488614
CAACGCA172850.028.2964945
AACGCAG176150.027.7641986
GTACATG103450.025.6370181
ACGCAGA193700.024.9496157
TACATGG104100.024.8853552
CGCAGAG194250.024.7321958
GAGTACT101000.024.58443812-13
GCAGAGT199000.023.7591639
ACATGGG104800.023.72133
AGTACTT104300.023.00924912-13
GTACTTT107450.022.77847914-15
CATGGGA61800.022.3052754
AGAGTAC172950.020.56490310-11
GTACCGT1404.8338828E-420.3692766
ACTTTTT122850.020.13556316-17
Produced by FastQC (version 0.11.5)