##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936800_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1904923 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01064504969492 33.0 31.0 34.0 30.0 34.0 2 32.10354329282601 33.0 31.0 34.0 30.0 34.0 3 32.12453364256718 34.0 31.0 34.0 30.0 34.0 4 35.74451355776585 37.0 35.0 37.0 35.0 37.0 5 35.751983675980604 37.0 35.0 37.0 35.0 37.0 6 35.828516953178685 37.0 35.0 37.0 35.0 37.0 7 35.780482990651066 37.0 35.0 37.0 35.0 37.0 8 35.75312965405951 37.0 35.0 37.0 35.0 37.0 9 37.41313218434551 39.0 37.0 39.0 35.0 39.0 10-11 37.30127569460813 39.0 37.0 39.0 34.0 39.0 12-13 37.21975192698078 39.0 37.0 39.0 34.0 39.0 14-15 38.39146437940011 40.0 38.0 41.0 34.0 41.0 16-17 38.34023606203505 40.0 38.0 41.0 34.0 41.0 18-19 38.33167324873499 40.0 38.0 41.0 34.0 41.0 20-21 38.28110060091667 40.0 38.0 41.0 34.0 41.0 22-23 38.17609950638425 40.0 38.0 41.0 34.0 41.0 24-25 38.13162526779298 40.0 38.0 41.0 33.5 41.0 26-27 38.13719294690652 40.0 38.0 41.0 34.0 41.0 28-29 38.06091479813095 40.0 38.0 41.0 33.5 41.0 30-31 37.877662509193286 40.0 37.5 41.0 33.0 41.0 32-33 37.65321957895411 40.0 37.0 41.0 33.0 41.0 34-35 37.47530320123175 40.0 36.5 41.0 33.0 41.0 36-37 37.2939533513953 40.0 36.0 41.0 32.5 41.0 38-39 37.12742168581092 39.0 36.0 41.0 32.0 41.0 40-41 36.93522572828403 39.0 36.0 41.0 31.0 41.0 42-43 36.68659704355504 39.0 35.0 41.0 31.0 41.0 44-45 36.38129913912531 39.0 35.0 41.0 30.0 41.0 46-47 35.96964155506548 39.0 35.0 40.0 29.5 41.0 48-49 35.663381407017496 39.0 35.0 40.0 28.0 41.0 50-51 34.93093395376086 38.0 34.0 40.0 27.0 40.5 52-53 35.09754252534092 38.5 35.0 40.0 27.5 40.5 54-55 35.466675293437056 39.0 35.0 40.5 28.0 41.0 56-57 35.33536998608342 39.0 35.0 41.0 27.5 41.0 58-59 35.11008870174805 38.0 35.0 40.0 27.0 41.0 60-61 34.816797056888916 38.0 34.5 40.0 26.0 41.0 62-63 34.498093623731776 37.5 34.0 40.0 26.0 41.0 64-65 34.14871388502317 37.0 34.0 40.0 26.0 41.0 66-67 33.77353152857097 36.0 34.0 39.0 25.5 41.0 68-69 33.37489415582677 36.0 33.5 39.0 24.5 41.0 70-71 32.955602667404406 35.0 33.0 38.5 23.5 40.0 72-73 32.58166918032907 35.0 33.0 37.5 22.5 40.0 74-75 32.20098082704655 35.0 32.5 37.0 21.5 39.0 76-77 31.765988441527558 35.0 32.0 36.5 20.0 39.0 78-79 31.340754718169713 35.0 32.0 36.0 18.5 38.5 80-81 30.940370293182454 35.0 31.0 36.0 17.5 37.0 82-83 30.575710934247738 35.0 31.0 35.0 13.0 37.0 84-85 30.242749706943535 34.0 31.0 35.0 8.5 36.5 86-87 29.914907059235468 34.0 31.0 35.0 6.0 36.0 88-89 29.573911123966692 34.0 30.0 35.0 2.0 36.0 90-91 29.239908647226162 34.0 30.0 35.0 2.0 35.0 92-93 28.881864253830734 34.0 29.0 35.0 2.0 35.0 94-95 28.538065580603522 34.0 29.0 35.0 2.0 35.0 96-97 28.089717537139293 34.0 29.0 35.0 2.0 35.0 98-99 27.570915464824566 34.0 28.5 35.0 2.0 35.0 100-101 25.43325347008777 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3866921313366589 1101 2 0.28136490808323344 1101 3 0.3376301548247156 1101 4 0.372450085945907 1101 5 0.3244847968029916 1101 6 0.2759093207782328 1101 7 0.21842296784985393 1101 8 0.308160608824096 1101 9 0.2097087224630414 1101 10-11 0.3700615204269866 1101 12-13 0.3747889359648937 1101 14-15 0.5209436596208619 1101 16-17 0.6163147793879205 1101 18-19 0.510978900872793 1101 20-21 0.5007850420745044 1101 22-23 0.5221493057173845 1101 24-25 0.5096339575062885 1101 26-27 0.511569506169991 1101 28-29 0.5122844255601251 1101 30-31 0.4781503611043405 1101 32-33 0.41276093037635064 1101 34-35 0.5279401151443395 1101 36-37 0.5559904161861695 1101 38-39 0.4053930173346032 1101 40-41 0.47431671448475754 1101 42-43 0.44768790451434626 1101 44-45 0.5659445295694283 1101 46-47 0.7193195984579148 1101 48-49 0.8780313494742913 1101 50-51 0.7737531286603883 1101 52-53 0.7316240665668374 1101 54-55 0.9175835849607736 1101 56-57 0.7685982621655825 1101 58-59 0.8967741377534892 1101 60-61 0.7857453734992603 1101 62-63 0.8811686414942628 1101 64-65 0.7949404967211038 1101 66-67 0.6820464500368715 1101 68-69 0.574205915394387 1101 70-71 0.6268307720320152 1101 72-73 0.5712154980526947 1101 74-75 0.5078138401223597 1101 76-77 0.5699837172890376 1101 78-79 0.630164704004418 1101 80-81 0.6576029369968666 1101 82-83 0.46365446279379796 1101 84-85 0.504046891048155 1101 86-87 0.6274990958897462 1101 88-89 0.6650208691187984 1101 90-91 0.774324367556904 1101 92-93 0.7579695216530773 1101 94-95 0.7981113641711737 1101 96-97 0.7496125922038352 1101 98-99 0.707542274377829 1101 100-101 1.0536864845061515 1102 1 0.17747227318063352 1102 2 0.13641809957259454 1102 3 0.19888902007294718 1102 4 0.15613802920831432 1102 5 0.21278266914341515 1102 6 0.1859270512746818 1102 7 0.10849388983566399 1102 8 0.014277630100686167 1102 9 0.19951368700204597 1102 10-11 0.1685987544695422 1102 12-13 0.08954957426276877 1102 14-15 0.1603496879896582 1102 16-17 0.24153641059359643 1102 18-19 0.0688246455536472 1102 20-21 0.028710573989400245 1102 22-23 -0.005332963786166545 1102 24-25 -0.06793696447952158 1102 26-27 0.03439752035438204 1102 28-29 -0.05509146096469664 1102 30-31 -0.07171666017225675 1102 32-33 0.011830079312524333 1102 34-35 -0.09528683521026693 1102 36-37 -0.0020769597003535978 1102 38-39 0.030171386128927224 1102 40-41 0.017091537179794614 1102 42-43 -0.09242734371260752 1102 44-45 -0.040216817948298456 1102 46-47 0.08456782540827135 1102 48-49 0.06752603032536086 1102 50-51 0.05921412156818917 1102 52-53 0.3672623644391777 1102 54-55 0.0889045069465908 1102 56-57 0.07843868769749918 1102 58-59 -0.08815494026068649 1102 60-61 0.08938012527230654 1102 62-63 -0.07627816701637613 1102 64-65 0.08616390097642324 1102 66-67 -0.32105638684256377 1102 68-69 -0.11661855269072419 1102 70-71 -0.10277915704599394 1102 72-73 -0.24571712606078222 1102 74-75 -0.16129076980672252 1102 76-77 -0.20350919051238137 1102 78-79 -0.1364576364211132 1102 80-81 -0.14026940342866112 1102 82-83 -0.12388986344733866 1102 84-85 -0.23974743519298158 1102 86-87 -0.22001863460670634 1102 88-89 -0.12221317343439608 1102 90-91 -0.3189823700317511 1102 92-93 -0.13121487551004307 1102 94-95 0.050992569844932945 1102 96-97 -0.26257712410822265 1102 98-99 -0.2281846759767845 1102 100-101 -0.2601433027278901 1103 1 0.18996973034541753 1103 2 0.20407658755654268 1103 3 0.14718624514622292 1103 4 0.10602163670552045 1103 5 0.06118403487709401 1103 6 0.09420062798046303 1103 7 0.10453111231987577 1103 8 0.11350830686472335 1103 9 0.07322435396460492 1103 10-11 0.20113664271424625 1103 12-13 0.08137118050242265 1103 14-15 0.31034402440213427 1103 16-17 0.33458770882656097 1103 18-19 0.32281053975319907 1103 20-21 0.2679194170456114 1103 22-23 0.25754013948993304 1103 24-25 0.19517424063943167 1103 26-27 0.31229877975131615 1103 28-29 0.23718721990486102 1103 30-31 0.10562326835095348 1103 32-33 0.23002354515067935 1103 34-35 0.27633032707809946 1103 36-37 0.15621568826953336 1103 38-39 0.3532888789858717 1103 40-41 0.3244703248484342 1103 42-43 0.31069583584854854 1103 44-45 0.138082452951501 1103 46-47 0.24284775920208546 1103 48-49 0.29311105579994745 1103 50-51 0.25275219936970217 1103 52-53 0.28228976926779126 1103 54-55 0.4430289856653715 1103 56-57 0.3851719419717696 1103 58-59 0.4367327704478825 1103 60-61 0.34117571368437893 1103 62-63 0.3308326404956219 1103 64-65 0.41456500086824377 1103 66-67 0.4244832490234316 1103 68-69 0.5089423158493247 1103 70-71 0.4083272536727449 1103 72-73 0.16362328560112616 1103 74-75 0.19825254854699637 1103 76-77 0.34480496753175416 1103 78-79 0.5064575045444215 1103 80-81 0.33677919708730286 1103 82-83 0.44238913643245326 1103 84-85 0.34852477762845524 1103 86-87 0.41918021048376986 1103 88-89 0.2589147704752861 1103 90-91 0.45781160021245526 1103 92-93 0.5713415985484112 1103 94-95 0.6754077177454754 1103 96-97 0.6426632547279922 1103 98-99 0.5330682367253736 1103 100-101 0.7736951879375908 1104 1 0.06512797080897315 1104 2 0.03648748486337894 1104 3 0.02532048659483621 1104 4 0.17176688069648804 1104 5 0.1979116345186327 1104 6 0.028581766042243828 1104 7 0.008009830901464454 1104 8 0.03947786848343071 1104 9 0.08527347738748858 1104 10-11 0.09445087916493122 1104 12-13 -0.0659562597179999 1104 14-15 -0.14566460093397637 1104 16-17 0.1627212141618486 1104 18-19 0.2076784972758361 1104 20-21 0.09792045142822303 1104 22-23 0.06943173466279262 1104 24-25 0.015502650426256537 1104 26-27 0.02052611754683653 1104 28-29 0.1257079246693138 1104 30-31 0.2680839555978167 1104 32-33 -0.050583042814956514 1104 34-35 0.09418912448307992 1104 36-37 0.13653020176651154 1104 38-39 0.20707794103028476 1104 40-41 0.09017396409707601 1104 42-43 0.13066469729921693 1104 44-45 0.10092345266604497 1104 46-47 0.11633138966708856 1104 48-49 0.040911271968703034 1104 50-51 -0.02998146928715073 1104 52-53 0.14143381670178456 1104 54-55 0.35284630910764037 1104 56-57 0.28472000663425945 1104 58-59 0.2952495919755833 1104 60-61 0.33183491367642404 1104 62-63 0.2488668049486833 1104 64-65 0.14799179191320633 1104 66-67 0.0020337430510650734 1104 68-69 0.0012341578208889814 1104 70-71 0.07558472297634466 1104 72-73 0.06463306099478672 1104 74-75 0.17510025511425908 1104 76-77 0.0472060162159984 1104 78-79 0.13036459136545275 1104 80-81 0.04733102778200049 1104 82-83 0.04315941688015812 1104 84-85 0.09936332541645498 1104 86-87 0.192280736986433 1104 88-89 0.04631673595520169 1104 90-91 -0.045150921294958124 1104 92-93 0.17424928439360343 1104 94-95 0.1955616399962281 1104 96-97 0.12106454279246037 1104 98-99 0.16395425139448605 1104 100-101 0.18145282807683571 1105 1 0.04589317961059436 1105 2 0.1991157223844553 1105 3 0.19926997971027305 1105 4 0.03515913754688427 1105 5 0.09481018395322138 1105 6 0.12529216616576377 1105 7 0.08102550948556342 1105 8 0.10714289102629238 1105 9 0.07709270438015636 1105 10-11 0.13708942413309444 1105 12-13 0.11295061523497907 1105 14-15 0.26954581553494705 1105 16-17 0.28190801290983103 1105 18-19 0.1351255116018848 1105 20-21 0.21443891325935738 1105 22-23 0.2180415290613169 1105 24-25 0.1595899884148153 1105 26-27 0.24410191154913718 1105 28-29 0.25411244450651793 1105 30-31 0.2583410155294956 1105 32-33 0.32983077932585303 1105 34-35 0.10011748201482362 1105 36-37 0.11441970372815291 1105 38-39 0.28507187629700326 1105 40-41 0.31315610507632385 1105 42-43 0.4689781909959905 1105 44-45 0.48114132696874634 1105 46-47 0.4670163799839102 1105 48-49 0.5108457840349558 1105 50-51 0.43270179117459406 1105 52-53 0.5679386074787303 1105 54-55 0.42309341213208285 1105 56-57 0.41233453174532286 1105 58-59 0.36232883879542044 1105 60-61 0.5898884682883647 1105 62-63 0.4195562774492245 1105 64-65 0.596610330114764 1105 66-67 0.6026508901214456 1105 68-69 0.5070097941984244 1105 70-71 0.5939857019671919 1105 72-73 0.5387140853273777 1105 74-75 0.7594114896013977 1105 76-77 0.8355064335947411 1105 78-79 0.7376481005135602 1105 80-81 0.6002343839450646 1105 82-83 0.5185882472468322 1105 84-85 0.5964057931224787 1105 86-87 0.7177758154463092 1105 88-89 0.7004471391429874 1105 90-91 0.7004310949866301 1105 92-93 0.7448438748153556 1105 94-95 0.5824892015268048 1105 96-97 0.6309563638312312 1105 98-99 0.66373171766881 1105 100-101 0.8644168079408843 1106 1 0.25146630485432553 1106 2 0.25307309131033406 1106 3 0.193906006453048 1106 4 0.18162080676371062 1106 5 0.20800813639716864 1106 6 0.29889738412562394 1106 7 0.30965868851370715 1106 8 0.31380755384159187 1106 9 0.3611505120371987 1106 10-11 0.4268058446153873 1106 12-13 0.31726874218673373 1106 14-15 0.2702755586057819 1106 16-17 0.369651114845837 1106 18-19 0.4300655493727561 1106 20-21 0.46072239517567226 1106 22-23 0.3237993195733466 1106 24-25 0.2558915654544762 1106 26-27 0.15739161361013032 1106 28-29 0.17775858162954705 1106 30-31 0.31202317879069597 1106 32-33 0.28755437394153915 1106 34-35 0.35968803161856755 1106 36-37 0.26461818866105347 1106 38-39 0.24838671287298553 1106 40-41 0.4464694430824352 1106 42-43 0.3722688258843405 1106 44-45 0.33308229886257124 1106 46-47 0.2390515112988041 1106 48-49 0.22466255514805766 1106 50-51 0.33391429399790695 1106 52-53 0.32326295716634235 1106 54-55 0.316183299117462 1106 56-57 0.27062758291565814 1106 58-59 0.34761402700845423 1106 60-61 0.37448124811821515 1106 62-63 0.34702204030983097 1106 64-65 0.2180594860571503 1106 66-67 0.20075776881630958 1106 68-69 0.3621449440402955 1106 70-71 0.4612212637646209 1106 72-73 0.3356808632855248 1106 74-75 0.5807462230394975 1106 76-77 0.5366444181953192 1106 78-79 0.5625546774215024 1106 80-81 0.6124876759403257 1106 82-83 0.4196063030705055 1106 84-85 0.5875085203037287 1106 86-87 0.7489767313445803 1106 88-89 0.7946866710709486 1106 90-91 0.9183362195342006 1106 92-93 0.9016994433412435 1106 94-95 1.0347669845589316 1106 96-97 1.0634577749485494 1106 98-99 1.1180536234351095 1106 100-101 1.436364033232941 1107 1 0.27776290847424434 1107 2 0.37820399779256775 1107 3 0.2662649065977689 1107 4 0.18158504694417843 1107 5 0.24997848785780974 1107 6 0.2612233195550644 1107 7 0.31638664313230436 1107 8 0.2786578406134552 1107 9 0.28688466478125463 1107 10-11 0.31334103191291973 1107 12-13 0.32515159301792806 1107 14-15 0.36085518149294415 1107 16-17 0.4197309176267794 1107 18-19 0.473771346769297 1107 20-21 0.4839407351464686 1107 22-23 0.5390690544325238 1107 24-25 0.5618960040508583 1107 26-27 0.570234716307219 1107 28-29 0.4979250034509235 1107 30-31 0.5505094291400852 1107 32-33 0.5281942411777365 1107 34-35 0.4324391950898345 1107 36-37 0.41668061800803713 1107 38-39 0.5477577049452478 1107 40-41 0.6154491862393527 1107 42-43 0.6025511168189368 1107 44-45 0.5822319084602228 1107 46-47 0.7226618372750906 1107 48-49 0.7007089495000471 1107 50-51 0.5868445922712908 1107 52-53 0.6717672114901774 1107 54-55 0.7303407576305929 1107 56-57 0.6918129406561562 1107 58-59 0.6608696812562513 1107 60-61 0.7632335442270417 1107 62-63 0.6927472458010797 1107 64-65 0.7619670710067865 1107 66-67 0.6914233612288072 1107 68-69 0.6993903286517522 1107 70-71 0.7356278879427904 1107 72-73 0.7933416018498676 1107 74-75 0.8145661818354242 1107 76-77 0.8035491430891817 1107 78-79 0.6905000276597413 1107 80-81 0.773289732202926 1107 82-83 0.8655965691016689 1107 84-85 0.7141975332086474 1107 86-87 0.8004726959584154 1107 88-89 0.7690034578249296 1107 90-91 0.7554783385443393 1107 92-93 0.9046229910364509 1107 94-95 0.9717216929230084 1107 96-97 0.8468298967391945 1107 98-99 1.0958022581762847 1107 100-101 1.1554567847175221 1108 1 -0.027253991682666623 1108 2 0.07053678184736611 1108 3 0.08999477029045266 1108 4 0.03325547232572035 1108 5 0.10156994273354769 1108 6 0.14216930654110627 1108 7 0.09785064851635639 1108 8 0.06205439721819772 1108 9 0.06296414707217934 1108 10-11 -0.03235071691119629 1108 12-13 0.08528090780702513 1108 14-15 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14 7886.0 15 10623.0 16 14760.0 17 21206.0 18 18411.0 19 9550.0 20 9034.0 21 9279.0 22 9911.0 23 11007.0 24 12118.0 25 14263.0 26 16633.0 27 20069.0 28 24212.0 29 30607.0 30 38595.0 31 49672.0 32 65303.0 33 88241.0 34 125031.0 35 188411.0 36 282781.0 37 406080.0 38 338179.0 39 54026.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9111703462842 18.984610407955998 14.788207999479067 22.316011246280734 2 15.369662472957929 20.740690176639852 40.125496208859296 23.76415114154292 3 18.47450774481491 26.062588605933318 30.20294040430559 25.25996324494618 4 12.731156276374438 16.965981800029304 37.35899276759403 32.94386915600222 5 13.50705496748105 37.45753200665406 33.70910436013489 15.326308665729998 6 30.567735615850943 36.50752944935384 17.826107976760024 15.098626958035197 7 27.55878464721333 33.6216300820246 21.259692395930337 17.559892874831732 8 26.340817065283577 33.52947635592183 20.303377215080808 19.826329363713786 9 27.170808606802595 16.244365450288576 20.660181955047573 35.92464398786126 10-11 24.464547145538432 26.685862931003236 27.38196608759191 21.467623835866426 12-13 26.030160467808734 24.633736481474866 27.81732682525041 21.51877622546599 14-15 23.33152028465302 25.099282143028795 26.939399613875786 24.629797958442403 16-17 21.468387697881344 28.576924480840077 27.247049456319505 22.70763836495907 18-19 21.466760861515187 26.679657852094827 30.60886989818027 21.244711388209712 20-21 22.509769523289282 25.773762001806837 30.50704351114566 21.20942496375822 22-23 22.293156851825742 25.689853494827986 30.478925588479548 21.53806406486672 24-25 21.960946137926474 25.928125101719175 30.591535704873174 21.51939305548118 26-27 21.71681007102847 26.11571918293637 30.563844251838706 21.603626494196455 28-29 21.61331331023905 26.049447173076658 30.676283421807167 21.660956094877125 30-31 21.63450849537977 26.087216729896294 30.535882607865954 21.74239216685798 32-33 21.593758941203955 26.237331380377523 30.30942799919152 21.85948167922701 34-35 21.980563848927 26.032036942441756 30.2300345285413 21.757364680089943 36-37 22.102038362820917 25.880073267034614 30.094607712148118 21.92328065799635 38-39 21.904922646525897 26.161877030825632 30.12453149431756 21.80866882833091 40-41 21.75022594170376 26.17051977222871 30.189014048940955 21.890240237126573 42-43 21.697462439516585 26.269055852369284 29.79269878484572 22.24078292326842 44-45 21.84983530532102 26.337840771257742 29.606116053511794 22.20620786990945 46-47 22.06948993763172 26.546430786586868 29.169463190743635 22.21461608503778 48-49 22.197231379795475 26.732119984041326 28.677556012850935 22.393092623312267 50-51 22.117223505176938 26.96810473670147 28.50299570946579 22.411676048655803 52-53 22.26300182248239 27.068969002049805 28.259711746966047 22.408317428501757 54-55 22.0327116787823 27.050688244145277 28.289727211920816 22.626872865151608 56-57 22.149673306756178 27.30284546389758 28.02949544787201 22.51798578147423 58-59 22.26408962932514 26.866354511679035 28.220653572536662 22.648902286459162 60-61 22.180755648253093 26.847713846691732 28.584751488413186 22.386779016641984 62-63 22.15095672918162 26.87102544085352 28.711955400412965 22.2660624295519 64-65 22.11182719719625 26.913713303723362 28.496781812364212 22.477677686716174 66-67 21.945887564651624 27.137917428116946 28.250315234510058 22.665879772721365 68-69 21.873591867730887 27.01878244940702 28.598779067263692 22.5088466155984 70-71 21.967011455070796 26.948226063398135 28.478212569851035 22.606549911680034 72-73 21.992870885863304 27.092129888469785 28.111965939843508 22.803033285823403 74-75 21.94773836737394 27.50211845942061 27.892287757667145 22.657855415538307 76-77 22.115109968580406 27.172015978627957 28.034295873447924 22.678578179343717 78-79 21.892389229418963 27.244862141109934 28.083653917595253 22.779094711875853 80-81 21.931469035140186 27.462241355352717 27.799330130293885 22.80695947921321 82-83 21.8874712242909 27.248076018282024 27.959224610162657 22.905228147264417 84-85 21.902544347244007 27.250779317088536 27.688876930245378 23.15779940542208 86-87 21.96511440150076 27.162220527287932 27.88555780331265 22.98710726789866 88-89 22.111708829348146 26.95438195508308 27.806966361411966 23.12694285415681 90-91 21.871881005766586 27.019243923631397 27.956140228799107 23.152734841802904 92-93 21.899771551610954 27.122968253551456 27.810204028044012 23.167056166793582 94-95 21.884965490592208 26.92243074755158 27.844020416884984 23.348583344971225 96-97 21.890517354760515 26.968639175815245 27.63423073823329 23.50661273119095 98-99 21.79722212448819 27.0609438517094 27.59981431116848 23.54201971263393 100-101 21.864069856259253 26.980440919831516 27.67673934079512 23.478749883114112 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2209.0 1 1521.0 2 1094.0 3 1254.5 4 1033.5 5 1135.0 6 2022.5 7 4207.5 8 6889.5 9 7513.5 10 5712.5 11 4219.0 12 4080.0 13 4163.5 14 4221.0 15 4209.5 16 4059.5 17 4063.0 18 4167.0 19 4381.0 20 4737.0 21 5097.5 22 5669.5 23 6624.5 24 7531.5 25 8804.0 26 10952.0 27 13099.0 28 14702.0 29 16727.0 30 19587.5 31 23127.0 32 27039.0 33 30800.0 34 34835.5 35 38537.5 36 43144.5 37 47664.0 38 49986.0 39 50532.5 40 50902.5 41 52467.0 42 53774.0 43 56604.0 44 60921.5 45 64277.5 46 65075.5 47 66684.0 48 72796.0 49 72851.0 50 68278.0 51 66034.5 52 64808.0 53 65526.0 54 64611.5 55 60972.0 56 56815.0 57 52602.0 58 49108.5 59 44763.5 60 38476.0 61 31373.5 62 24796.0 63 19316.0 64 14303.0 65 10505.5 66 7582.5 67 5791.0 68 4324.0 69 2885.0 70 1935.5 71 1155.5 72 731.0 73 572.5 74 390.5 75 245.5 76 161.0 77 77.5 78 44.0 79 32.0 80 22.5 81 15.0 82 9.5 83 9.5 84 12.5 85 10.5 86 5.5 87 3.0 88 3.5 89 3.0 90 1.0 91 0.0 92 1.5 93 3.0 94 2.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03406961856201012 2 0.0253553555708026 3 0.022205621959522772 4 0.04483120840054952 5 0.090554841324295 6 0.10472864257505421 7 0.05155064010461315 8 0.08136811829139551 9 0.06645937919800433 10-11 0.06582943247574836 12-13 0.0650419990729284 14-15 0.05939872635271872 16-17 0.04774471199098336 18-19 0.0560652582807809 20-21 0.05475286927608098 22-23 0.0308411416104483 24-25 0.00887174967177151 26-27 0.0027822646899638465 28-29 0.006430706123029645 30-31 0.004908334877577729 32-33 0.006194476102183657 34-35 0.006404458342935646 36-37 0.005774511620679682 38-39 0.003333468071937816 40-41 0.006168228322089659 42-43 0.00325472473165582 44-45 0.0018110968264859 46-47 0.0014961234653579174 48-49 1.574866805639913E-4 50-51 0.0016798579260159073 52-53 0.0054332904794577 54-55 0.011601518801547358 56-57 0.008110564049045551 58-59 0.00524955601879971 60-61 0.002204813527895878 62-63 0.007979325148575559 64-65 0.002441043548741865 66-67 0.020394525133036873 68-69 0.04225892595133767 70-71 0.05512033819739696 72-73 0.003044742490903832 74-75 0.012441447764555313 76-77 0.0198958173112509 78-79 0.024016718786008674 80-81 0.07446495212667388 82-83 0.028295106941330434 84-85 0.01105031541957339 86-87 0.02916128368443239 88-89 0.04357131495603759 90-91 0.04008036020353579 92-93 0.04057906802532176 94-95 0.011811501042299347 96-97 0.015827411396681125 98-99 0.034752060844454084 100-101 0.07123647517511206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1904923.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.05890809337968 #Duplication Level Percentage of deduplicated Percentage of total 1 65.9924938003157 27.095797378000903 2 15.710100444476078 12.900791405750129 3 6.648155413510171 8.18898006341456 4 3.4446138296747133 5.657283305991946 5 2.096922193400349 4.304866780889652 6 1.3678810154468024 3.3698220537545476 7 0.89840687197483 2.5821323630812683 8 0.6620685947473894 2.1747050866596878 9 0.4741820877085796 1.7522458882878122 >10 2.4454706112638 18.04369971325977 >50 0.17636380218643835 4.986200022852853 >100 0.07976275175878224 5.654963956208982 >500 0.001917098323070718 0.5193022575257494 >1k 0.0012780655487138121 0.8808617314902212 >5k 1.278065548713812E-4 0.43051290629964106 >10k+ 2.556131097427624E-4 1.4578350865322194 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16756 0.8796156065100794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11025 0.578763551072668 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8204 0.43067357578232823 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2503 0.13139638715055674 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2444 0.1282991490994649 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2158 0.11328541888569774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2111 0.11081812755686189 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.24955601879971E-5 2 0.0 0.0 0.0 5.24955601879971E-5 5.24955601879971E-5 3 0.0 0.0 0.0 5.24955601879971E-5 5.24955601879971E-5 4 0.0 0.0 0.0 1.049911203759942E-4 5.24955601879971E-5 5 5.24955601879971E-5 0.0 0.0 1.574866805639913E-4 5.24955601879971E-5 6 5.24955601879971E-5 0.0 0.0 1.574866805639913E-4 5.24955601879971E-5 7 5.24955601879971E-5 0.0 0.0 2.099822407519884E-4 5.24955601879971E-5 8 1.049911203759942E-4 0.0 0.0 2.099822407519884E-4 5.24955601879971E-5 9 1.049911203759942E-4 0.0 0.0 2.624778009399855E-4 5.24955601879971E-5 10-11 2.099822407519884E-4 0.0 0.0 2.624778009399855E-4 7.874334028199566E-5 12-13 2.8872558103398404E-4 0.0 0.0 3.149733611279826E-4 2.624778009399855E-4 14-15 5.24955601879971E-4 0.0 0.0 3.937167014099783E-4 4.1996448150397677E-4 16-17 5.24955601879971E-4 0.0 0.0 6.299467222559653E-4 4.724600416919739E-4 18-19 5.24955601879971E-4 0.0 0.0 9.186723032899493E-4 5.774511620679681E-4 20-21 5.24955601879971E-4 0.0 0.0 0.0010761589838539406 6.824422824439623E-4 22-23 5.24955601879971E-4 0.0 0.0 0.0014961234653579174 6.824422824439623E-4 24-25 6.824422824439623E-4 0.0 0.0 0.002152317967707881 6.824422824439623E-4 26-27 6.824422824439623E-4 0.0 0.0 0.0031234858311858273 6.824422824439623E-4 28-29 7.874334028199566E-4 0.0 0.0 0.005459538259551698 6.824422824439623E-4 30-31 8.924245231959507E-4 0.0 0.0 0.011155306539949384 6.824422824439623E-4 32-33 8.924245231959507E-4 0.0 0.0 0.021496931896984813 7.349378426319594E-4 34-35 9.186723032899493E-4 0.0 0.0 0.03464706972407809 7.349378426319594E-4 36-37 0.0011024067639479392 0.0 0.0 0.04829591537295733 7.349378426319594E-4 38-39 0.0012073978843239333 0.0 0.0 0.06480576905208242 7.349378426319594E-4 40-41 0.0012073978843239333 5.24955601879971E-5 0.0 0.08632894872916122 7.349378426319594E-4 42-43 0.0012598934445119305 5.24955601879971E-5 0.0 0.1139153656079537 7.349378426319594E-4 44-45 0.0014173801250759216 5.24955601879971E-5 0.0 0.1466988429453579 7.349378426319594E-4 46-47 0.0016011145857339117 5.24955601879971E-5 0.0 0.18764537989199565 7.349378426319594E-4 48-49 0.0016798579260159073 5.24955601879971E-5 0.0 0.23371023395696308 7.349378426319594E-4 50-51 0.0016798579260159073 5.24955601879971E-5 0.0 0.28872558103398405 7.349378426319594E-4 52-53 0.0017061057061099057 5.24955601879971E-5 0.0 0.35828219828308017 7.349378426319594E-4 54-55 0.0017323534862039042 5.24955601879971E-5 0.0 0.4332983537917281 7.349378426319594E-4 56-57 0.0018373446065798986 5.24955601879971E-5 0.0 0.5231182572733911 7.349378426319594E-4 58-59 0.0018373446065798986 5.24955601879971E-5 0.0 0.6288180677119233 7.349378426319594E-4 60-61 0.0018373446065798986 5.24955601879971E-5 0.0 0.7537312531792624 7.349378426319594E-4 62-63 0.0018373446065798986 5.24955601879971E-5 0.0 0.8777520141234055 7.349378426319594E-4 64-65 0.0018373446065798986 5.24955601879971E-5 0.0 1.01143195814214 7.349378426319594E-4 66-67 0.0018373446065798986 5.24955601879971E-5 0.0 1.1773704238963991 7.349378426319594E-4 68-69 0.0018373446065798986 5.24955601879971E-5 0.0 1.3679030595987345 7.349378426319594E-4 70-71 0.0019423357269558927 5.24955601879971E-5 0.0 1.5691185417993272 7.349378426319594E-4 72-73 0.0020735746274258855 5.24955601879971E-5 0.0 1.7748486421760878 7.349378426319594E-4 74-75 0.0021523179677078812 5.24955601879971E-5 0.0 2.015357051177397 7.61185622725958E-4 76-77 0.0022048135278958784 5.24955601879971E-5 0.0 2.2535819033105273 7.874334028199565E-4 78-79 0.002231061307989877 5.24955601879971E-5 0.0 2.5076604146204335 7.874334028199565E-4 80-81 0.0022835568681778737 5.24955601879971E-5 0.0 2.783682070088922 7.874334028199565E-4 82-83 0.0023623002084598695 5.24955601879971E-5 0.0 3.1104669322592042 8.399289630079536E-4 84-85 0.0024410435487418653 5.24955601879971E-5 0.0 3.4673842459773967 8.399289630079536E-4 86-87 0.002493539108929862 5.24955601879971E-5 0.0 3.8493156941251696 8.399289630079536E-4 88-89 0.0025197868890238606 5.24955601879971E-5 0.0 4.277994438620354 8.399289630079536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13615 0.0 36.026814 1 GGTATCA 8515 0.0 35.165737 1 ATCAACG 16920 0.0 28.99195 3 TATCAAC 16970 0.0 28.906525 2 TCAACGC 17030 0.0 28.748861 4 CAACGCA 17285 0.0 28.296494 5 AACGCAG 17615 0.0 27.764198 6 GTACATG 10345 0.0 25.637018 1 ACGCAGA 19370 0.0 24.949615 7 TACATGG 10410 0.0 24.885355 2 CGCAGAG 19425 0.0 24.732195 8 GAGTACT 10100 0.0 24.584438 12-13 GCAGAGT 19900 0.0 23.759163 9 ACATGGG 10480 0.0 23.7213 3 AGTACTT 10430 0.0 23.009249 12-13 GTACTTT 10745 0.0 22.778479 14-15 CATGGGA 6180 0.0 22.305275 4 AGAGTAC 17295 0.0 20.564903 10-11 GTACCGT 140 4.8338828E-4 20.369276 6 ACTTTTT 12285 0.0 20.135563 16-17 >>END_MODULE