FastQCFastQC Report
Thu 26 May 2016
SRR936800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904923
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153260.8045469554412435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110740.5813358335218799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85080.4466322260794793No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33470.1757026399492263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32260.16935067716647864No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23850.12520191104837308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.12016233727032537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145600.034.161241
GGTATCA93050.032.827891
ATCAACG183800.026.9530163
TATCAAC184850.026.8256072
TCAACGC184850.026.7742184
CAACGCA187200.026.4634845
AACGCAG191500.025.8699446
GTACATG100650.023.929991
ACGCAGA210600.023.3658377
GAGTACT112900.023.3306812-13
AGTACTT114750.023.24432212-13
CGCAGAG210850.023.1828758
TACATGG104500.022.6350572
GCAGAGT214200.022.376769
ACATGGG103350.022.1975573
GTACTTT121150.021.58451714-15
AGAGTAC182650.020.67630810-11
TACTTTT132600.020.0968414-15
TATAGGG3553.6379788E-1220.0692632
CATGGGG42350.019.8512734
Produced by FastQC (version 0.11.5)