##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936800_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1904923 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820153885485134 31.0 31.0 34.0 30.0 34.0 2 31.958104868280767 31.0 31.0 34.0 30.0 34.0 3 32.07105116584765 33.0 31.0 34.0 30.0 34.0 4 35.82907183124987 37.0 35.0 37.0 35.0 37.0 5 35.636299734949915 37.0 35.0 37.0 33.0 37.0 6 35.72674486055342 37.0 35.0 37.0 33.0 37.0 7 35.667457424788296 37.0 35.0 37.0 33.0 37.0 8 35.62054371751509 37.0 35.0 37.0 33.0 37.0 9 37.241590342496785 39.0 37.0 39.0 33.0 39.0 10-11 37.138941311538574 39.0 37.0 39.0 33.0 39.0 12-13 37.01958005651672 39.0 37.0 39.0 33.0 39.0 14-15 38.080629505759546 40.0 37.0 41.0 33.0 41.0 16-17 38.07535422691626 40.0 37.0 41.0 33.0 41.0 18-19 38.22268800366209 40.0 37.5 41.0 33.5 41.0 20-21 38.22589102026696 40.0 38.0 41.0 33.0 41.0 22-23 38.110209179058685 40.0 37.0 41.0 33.0 41.0 24-25 37.99626651575943 40.0 37.0 41.0 33.0 41.0 26-27 37.75062797813875 40.0 37.0 41.0 32.5 41.0 28-29 37.56044601277847 39.0 36.5 41.0 32.0 41.0 30-31 37.48924024750607 39.0 36.5 41.0 32.0 41.0 32-33 37.30364376932821 39.0 36.0 41.0 32.0 41.0 34-35 37.14342207007843 39.0 36.0 41.0 31.0 41.0 36-37 36.9509106142348 39.0 35.0 41.0 31.0 41.0 38-39 36.749917713209406 39.0 35.0 40.5 31.0 41.0 40-41 36.37087903290579 39.0 35.0 40.0 30.0 41.0 42-43 36.11148088400424 38.0 35.0 40.0 30.0 41.0 44-45 35.80452070766115 38.0 35.0 40.0 29.0 41.0 46-47 35.331530985766875 38.0 34.5 40.0 27.5 41.0 48-49 34.88574997519585 38.0 34.0 40.0 26.0 41.0 50-51 34.56153477069677 38.0 34.0 40.0 24.5 41.0 52-53 34.26014411081182 38.0 33.0 40.0 24.0 41.0 54-55 33.814529773644395 37.5 33.0 40.0 23.0 41.0 56-57 33.572276674700234 37.0 33.0 40.0 22.5 41.0 58-59 33.20374576820166 36.5 32.0 39.0 22.0 40.5 60-61 32.76992088394124 36.0 31.5 39.0 20.5 40.0 62-63 32.348110396063255 35.5 31.0 39.0 19.5 40.0 64-65 31.969894583665585 35.0 31.0 38.0 18.0 40.0 66-67 31.59364472999696 35.0 30.5 38.0 16.0 40.0 68-69 31.103375307033406 35.0 30.0 37.5 12.5 39.5 70-71 30.62834796997044 34.0 29.5 37.0 8.5 39.0 72-73 30.189930249149178 34.0 29.0 36.0 7.5 39.0 74-75 29.678773367742423 34.0 29.0 36.0 7.0 38.5 76-77 28.324973240388193 32.0 27.0 34.5 3.5 36.5 78-79 29.012407588128234 33.5 29.0 35.0 2.0 37.0 80-81 28.8786998214626 34.0 29.0 35.0 2.0 36.5 82-83 28.509668369797623 33.0 28.0 35.0 2.0 36.0 84-85 28.02921981623404 33.0 27.0 35.0 2.0 36.0 86-87 27.591769851064846 33.0 26.0 35.0 2.0 35.0 88-89 27.147114345304246 32.5 26.0 34.5 2.0 35.0 90-91 26.601389137513692 32.0 25.0 34.0 2.0 35.0 92-93 26.006202612914013 32.0 24.0 34.0 2.0 35.0 94-95 25.321496196959142 31.5 21.5 34.0 2.0 35.0 96-97 24.38654922009971 31.0 16.5 34.0 2.0 35.0 98-99 23.330814946325916 31.0 2.0 34.0 2.0 35.0 100-101 20.12106526090556 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28956995881980063 1101 2 0.43620464664008907 1101 3 0.2930014066331381 1101 4 0.22545024110204537 1101 5 0.17057401488843738 1101 6 0.16785957254835893 1101 7 0.2376587053075312 1101 8 0.22285919220396977 1101 9 0.27153357941685385 1101 10-11 0.2706178091355653 1101 12-13 0.2657757886926575 1101 14-15 0.5635682514160294 1101 16-17 0.5327520192927864 1101 18-19 0.32433583593129356 1101 20-21 0.12974990735988712 1101 22-23 0.3559630418271098 1101 24-25 0.32195564580187863 1101 26-27 0.25520855538898246 1101 28-29 0.39364197479581975 1101 30-31 0.49060967751781703 1101 32-33 0.5413582959530245 1101 34-35 0.30908000711135486 1101 36-37 0.37721747128576766 1101 38-39 0.44930114131491194 1101 40-41 0.6023766524123104 1101 42-43 0.5663222510351034 1101 44-45 0.444678691046839 1101 46-47 0.22790592382923336 1101 48-49 0.3452161688117883 1101 50-51 0.30025636271741973 1101 52-53 0.5732408380648693 1101 54-55 0.487885622159105 1101 56-57 0.5526535496190164 1101 58-59 0.5875041907157126 1101 60-61 0.47032041938659574 1101 62-63 0.5775770359210242 1101 64-65 0.5415417662113349 1101 66-67 0.5013143762417869 1101 68-69 0.20670317928194137 1101 70-71 0.13027364600152325 1101 72-73 0.27098546489904507 1101 74-75 0.11460876727135272 1101 76-77 0.16732874175495382 1101 78-79 0.3795407829281885 1101 80-81 0.2711913566493074 1101 82-83 0.44671209684706525 1101 84-85 0.39098370499266366 1101 86-87 0.036274252422387576 1101 88-89 0.2586154276223702 1101 90-91 0.3710844276262115 1101 92-93 0.19961138191618488 1101 94-95 0.10355812640984041 1101 96-97 0.13227353947342735 1101 98-99 0.2883287920840232 1101 100-101 0.6446499585720069 1102 1 -0.07966762983268083 1102 2 0.11528266082448724 1102 3 0.1715475059239182 1102 4 0.07429775883254308 1102 5 -0.04041889291298162 1102 6 0.06901205481786121 1102 7 0.04350976913731586 1102 8 0.10184855390609471 1102 9 0.20282790565798337 1102 10-11 0.2533305750930097 1102 12-13 0.23319600145861585 1102 14-15 0.25306293226708476 1102 16-17 0.22623606184598088 1102 18-19 0.22637484302348554 1102 20-21 0.2261594818279704 1102 22-23 0.03947368012497776 1102 24-25 0.023196780553647045 1102 26-27 0.2902262858854314 1102 28-29 0.044794457065322035 1102 30-31 0.2288632236171111 1102 32-33 0.05310474985373759 1102 34-35 0.27184596455816035 1102 36-37 0.24645505993824912 1102 38-39 0.3872444037262568 1102 40-41 0.3171372907101784 1102 42-43 0.286623669474821 1102 44-45 0.19312727260712137 1102 46-47 0.22480308694979811 1102 48-49 0.4269980837054064 1102 50-51 0.4549549442777021 1102 52-53 0.40635254019252187 1102 54-55 0.777114345563362 1102 56-57 0.6592581595480951 1102 58-59 0.44366553823344645 1102 60-61 0.5321555257695749 1102 62-63 0.4047046954954894 1102 64-65 0.8301055959985639 1102 66-67 0.6839384897169616 1102 68-69 0.6366677182890363 1102 70-71 0.3605484686965603 1102 72-73 0.30954929468628123 1102 74-75 0.33202898003731107 1102 76-77 0.41267448643580096 1102 78-79 0.0639379460487568 1102 80-81 0.36405483182661413 1102 82-83 0.5581925933009622 1102 84-85 0.41359008797138586 1102 86-87 0.2658841815004003 1102 88-89 0.3785179098918725 1102 90-91 0.2651447113141536 1102 92-93 0.14376031808639667 1102 94-95 0.15852266541693538 1102 96-97 0.35191006429612415 1102 98-99 0.16620999775778245 1102 100-101 0.1442066961606585 1103 1 0.14302867704591904 1103 2 0.17335466004776734 1103 3 0.14152760245919183 1103 4 0.08707723953936153 1103 5 -0.03850309774529137 1103 6 0.07124281480195549 1103 7 0.08454477241988911 1103 8 0.1851789976539564 1103 9 0.13756430611292103 1103 10-11 0.08205790918457012 1103 12-13 0.1564665267621379 1103 14-15 0.26400723724087527 1103 16-17 0.18662273461544032 1103 18-19 0.18443568844684677 1103 20-21 0.1633424943017232 1103 22-23 0.07718883865919679 1103 24-25 0.09052296620836842 1103 26-27 0.15197349107836544 1103 28-29 0.04725696180424421 1103 30-31 0.2609142167098426 1103 32-33 0.16085681417359865 1103 34-35 0.21275162999501873 1103 36-37 0.07515349817509787 1103 38-39 0.10745682293345027 1103 40-41 0.11918248151529554 1103 42-43 0.02002704635998498 1103 44-45 0.1589902877624212 1103 46-47 0.02820089271418169 1103 48-49 0.12805255123571158 1103 50-51 0.001873432539049702 1103 52-53 0.008216207238305628 1103 54-55 0.19040366473078763 1103 56-57 -0.10001336439616892 1103 58-59 -0.17508082088886567 1103 60-61 -0.4122624308080418 1103 62-63 -0.0640874924523942 1103 64-65 -0.17787572803261753 1103 66-67 -0.07728639400611925 1103 68-69 0.019955916297302423 1103 70-71 -0.003785493410571661 1103 72-73 0.02819238002382818 1103 74-75 -0.04569763281371664 1103 76-77 0.21831403260906868 1103 78-79 0.13447532803808215 1103 80-81 0.07314581447060675 1103 82-83 0.39531850756580766 1103 84-85 0.15614160350117245 1103 86-87 0.037665298868429176 1103 88-89 0.26110249383780015 1103 90-91 0.2313328048350094 1103 92-93 0.10337402674220897 1103 94-95 0.2809529570570177 1103 96-97 0.549330878095855 1103 98-99 0.2603928576614294 1103 100-101 0.7015805715339951 1104 1 0.04046935443842514 1104 2 0.11901343653565633 1104 3 0.011218146156309672 1104 4 0.05803823834379784 1104 5 -0.1590585745908939 1104 6 -0.035331097762693275 1104 7 0.01071088585437252 1104 8 0.05045917098665598 1104 9 0.11489794306910284 1104 10-11 0.07541912304881038 1104 12-13 0.13965905213366625 1104 14-15 0.21647993747468774 1104 16-17 0.0492838741226862 1104 18-19 0.1001976527260453 1104 20-21 0.1826857359115195 1104 22-23 0.17529324170666882 1104 24-25 0.1328961343616939 1104 26-27 0.037896671822103656 1104 28-29 0.08180209061737997 1104 30-31 0.13416936524890843 1104 32-33 0.21376797026729122 1104 34-35 0.3219012699782482 1104 36-37 0.22989541588986384 1104 38-39 0.13856717052615153 1104 40-41 0.3550763993646626 1104 42-43 0.3023155098582251 1104 44-45 0.47900656638265104 1104 46-47 0.12921734092726922 1104 48-49 0.25729722440426883 1104 50-51 0.3898409051255243 1104 52-53 0.28155028242090196 1104 54-55 0.17358634339170465 1104 56-57 0.18618366962163435 1104 58-59 -0.044744310031575196 1104 60-61 0.06825762082313247 1104 62-63 0.2570071443789317 1104 64-65 0.3340484473244878 1104 66-67 0.10054567770546896 1104 68-69 0.23420972778690086 1104 70-71 0.14377033313665777 1104 72-73 0.16931036981116776 1104 74-75 0.1458283453900613 1104 76-77 0.4457360509629531 1104 78-79 0.5575558838820314 1104 80-81 0.2997786619882703 1104 82-83 0.5929323341065356 1104 84-85 0.4930825612859344 1104 86-87 0.3298892845759802 1104 88-89 0.24909581642961243 1104 90-91 0.3236222950679313 1104 92-93 0.2257391951601555 1104 94-95 0.5659294769541603 1104 96-97 0.8971513995202933 1104 98-99 0.7958443618468536 1104 100-101 1.492667309965018 1105 1 0.06242594972756521 1105 2 0.15721322140659666 1105 3 0.1278756423328673 1105 4 0.06953377920118697 1105 5 0.0529166150649516 1105 6 0.052918097472641534 1105 7 0.11779332691936872 1105 8 0.10152672237914828 1105 9 0.2099379617225452 1105 10-11 0.18713804444163173 1105 12-13 0.1238276484106251 1105 14-15 0.1915238632689551 1105 16-17 0.0686493300352069 1105 18-19 0.07901628222550272 1105 20-21 0.11976757436305974 1105 22-23 0.1474890312110304 1105 24-25 0.02411505201278885 1105 26-27 0.06845301401359194 1105 28-29 0.047543318948406466 1105 30-31 0.17762990647455723 1105 32-33 0.1526459315905342 1105 34-35 0.11766286900714107 1105 36-37 0.14138321729998182 1105 38-39 0.09432096708936655 1105 40-41 0.1761034535577295 1105 42-43 0.1881670322055342 1105 44-45 0.17178123109521692 1105 46-47 0.15618074811160199 1105 48-49 0.1279054819415677 1105 50-51 0.3220383252309915 1105 52-53 0.5590030668692307 1105 54-55 0.5816201456738455 1105 56-57 0.3154686746690132 1105 58-59 0.37488684721076027 1105 60-61 0.3987408813636506 1105 62-63 0.4291073428475016 1105 64-65 0.4337664990633847 1105 66-67 0.43369205645156583 1105 68-69 0.4081357859347641 1105 70-71 0.3156595184308415 1105 72-73 0.32689585525568887 1105 74-75 0.2525131797363507 1105 76-77 0.08905879851793586 1105 78-79 0.4418973278147611 1105 80-81 0.3346745102966793 1105 82-83 0.28576657090053814 1105 84-85 0.037593442003526434 1105 86-87 0.07774528044197027 1105 88-89 0.5133165092302754 1105 90-91 0.5175084468138778 1105 92-93 0.5760385916408133 1105 94-95 0.5899096521531924 1105 96-97 0.6038364898788302 1105 98-99 0.8180480089579945 1105 100-101 0.9961177643079893 1106 1 -0.0037350467739010185 1106 2 0.105922831817292 1106 3 0.08081342231603372 1106 4 0.21082322300718914 1106 5 0.008820440564043963 1106 6 0.14850677415770264 1106 7 0.17392578149478055 1106 8 0.17908462353485533 1106 9 0.37740465741050855 1106 10-11 0.2595696057916612 1106 12-13 0.27871218727216274 1106 14-15 0.4204964878089612 1106 16-17 0.41960046845167653 1106 18-19 0.2214101115725171 1106 20-21 0.18943509482588183 1106 22-23 0.27448775418765337 1106 24-25 0.3540065330964808 1106 26-27 0.5126028908674201 1106 28-29 0.609912976838288 1106 30-31 0.4958608296152036 1106 32-33 0.525698816423926 1106 34-35 0.5511526020759945 1106 36-37 0.5178188969100574 1106 38-39 0.6409271381742272 1106 40-41 0.6619880697230229 1106 42-43 0.6627860305303344 1106 44-45 0.5375396003056991 1106 46-47 0.7320319206885557 1106 48-49 0.43044860253411343 1106 50-51 0.684524780880011 1106 52-53 0.5658577284796209 1106 54-55 0.6821086492244106 1106 56-57 0.754888015548751 1106 58-59 0.7295107774283593 1106 60-61 0.4986436686604421 1106 62-63 0.44896069461281485 1106 64-65 0.520450237334483 1106 66-67 0.5700226159190969 1106 68-69 0.6873763608155379 1106 70-71 0.5207118150647609 1106 72-73 0.6355514782828777 1106 74-75 0.4434001042174991 1106 76-77 0.7526689440519618 1106 78-79 0.6096141810253037 1106 80-81 0.6050029130327985 1106 82-83 0.7622501442857619 1106 84-85 0.7440685162950196 1106 86-87 0.7013571059289738 1106 88-89 0.979275112424947 1106 90-91 0.7947756167754534 1106 92-93 0.9938539099616719 1106 94-95 1.0797499313795136 1106 96-97 1.0915729411012443 1106 98-99 1.1074835015707372 1106 100-101 1.4642081587341735 1107 1 0.3104281041186745 1107 2 0.38928656099723113 1107 3 0.29714500487779105 1107 4 0.18584900291790518 1107 5 0.1024235926826691 1107 6 0.20140065579853683 1107 7 0.18647966752896394 1107 8 0.12435166546069354 1107 9 0.32397802743779636 1107 10-11 0.2813841903975671 1107 12-13 0.3065515716778151 1107 14-15 0.3636146564975178 1107 16-17 0.4421437506046786 1107 18-19 0.4491242636305657 1107 20-21 0.41549590257452707 1107 22-23 0.34689628020438334 1107 24-25 0.39280520750186554 1107 26-27 0.5843392782352979 1107 28-29 0.470996295028435 1107 30-31 0.48881651006253435 1107 32-33 0.4884615325062853 1107 34-35 0.6044000680549075 1107 36-37 0.6056920852920555 1107 38-39 0.579612913720787 1107 40-41 0.8100085447739787 1107 42-43 0.8237705348517963 1107 44-45 0.5996149980607086 1107 46-47 0.5128760421756056 1107 48-49 0.6526159687066411 1107 50-51 0.38358801956658795 1107 52-53 0.5094174214706229 1107 54-55 0.7940562238693829 1107 56-57 0.5689446167749495 1107 58-59 0.5747940937210458 1107 60-61 0.5519444094725401 1107 62-63 0.5933705225788941 1107 64-65 0.7316761934170479 1107 66-67 0.7525261389911115 1107 68-69 0.5616433757390595 1107 70-71 0.5328548280703878 1107 72-73 0.7153017141882181 1107 74-75 0.7605061422995156 1107 76-77 0.46197116780188097 1107 78-79 0.930087115585561 1107 80-81 0.9231817457009264 1107 82-83 0.910465838759702 1107 84-85 0.7889605909702802 1107 86-87 0.9691109440887864 1107 88-89 1.0939568536800302 1107 90-91 1.1597625936330154 1107 92-93 1.265344649263099 1107 94-95 1.4266920654233743 1107 96-97 1.2963329379777342 1107 98-99 1.7221002913168633 1107 100-101 2.0846143902841128 1108 1 0.023480686758844627 1108 2 0.13256000797310818 1108 3 0.02608987456005707 1108 4 0.06105756004006224 1108 5 0.0033289649266663446 1108 6 0.012590846771246333 1108 7 0.08936023975346785 1108 8 0.10428237762936732 1108 9 0.14009391583489617 1108 10-11 0.15762873744899508 1108 12-13 0.1581874328172077 1108 14-15 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2316 58-59 -1.8079966958091092 2316 60-61 -1.6376139777765246 2316 62-63 -1.5885355527314609 2316 64-65 -1.853737489107818 2316 66-67 -2.0068859783681425 2316 68-69 -2.026452849086855 2316 70-71 -1.9589329142821619 2316 72-73 -1.9971882939662002 2316 74-75 -2.0323327220903487 2316 76-77 -2.0497590242024977 2316 78-79 -2.018398046859044 2316 80-81 -2.0185914447691324 2316 82-83 -2.0571664492522252 2316 84-85 -1.7831076070640783 2316 86-87 -1.7120635135350604 2316 88-89 -2.689744501455646 2316 90-91 -2.7451611397496762 2316 92-93 -2.6666341854596993 2316 94-95 -2.8811493203986664 2316 96-97 -2.581711389604589 2316 98-99 -2.6938298958592384 2316 100-101 -3.1634174173145198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 28.0 5 72.0 6 177.0 7 401.0 8 884.0 9 1658.0 10 2773.0 11 4397.0 12 6403.0 13 8680.0 14 11064.0 15 15036.0 16 19465.0 17 25929.0 18 22065.0 19 15032.0 20 13835.0 21 14154.0 22 15722.0 23 17451.0 24 20277.0 25 23964.0 26 28595.0 27 35219.0 28 43056.0 29 52617.0 30 64642.0 31 77632.0 32 96826.0 33 121527.0 34 159380.0 35 215852.0 36 293712.0 37 334419.0 38 138317.0 39 3653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47976166598584 20.128124484787516 15.528149897561319 21.863963951665326 2 15.987591810679044 21.896705866171516 38.647551461490046 23.4681508616594 3 18.935083329002442 26.215077911449253 30.50203184492359 24.347806914624716 4 13.493668772963527 17.863609185253157 36.66237427969529 31.980347762088023 5 13.570754739267452 37.95676689854442 33.53891026377998 14.933568098408143 6 30.759038554314266 37.10706417004781 17.206364771699434 14.92753250393848 7 27.520172773750485 33.96217896928951 21.038742329726357 17.47890592723365 8 26.69131508202694 33.96625480399995 19.90022693830669 19.442203175666418 9 27.158568338827877 16.256798185750583 20.537345400331773 36.04728807508977 10-11 24.735573231981878 27.057058751733994 26.987397125079895 21.219970891204234 12-13 26.204723847262567 24.815199510506087 27.73265988546451 21.24741675676683 14-15 23.352330384905535 25.38613134739248 26.867843622907127 24.39369464479486 16-17 21.448767220512323 28.865786176134158 27.278110453808367 22.407336149545152 18-19 21.522893296729748 26.82449885519736 30.66866290779083 20.983944940282065 20-21 22.55374169444154 25.87612256607831 30.702310201223266 20.867825538256884 22-23 22.35026942408682 25.796755886737156 30.633002895432902 21.219971793743124 24-25 22.110105493200596 26.013545525391102 30.75384946108988 21.12249952031842 26-27 21.891499238155983 26.232737113287058 30.70324567861863 21.172517969938333 28-29 21.69435506329795 26.213417417376426 30.846684072476556 21.245543446849066 30-31 21.78796728266707 26.15040082984982 30.703891968336777 21.357739919146336 32-33 21.774956428616424 26.325409990970748 30.448680259538456 21.450953320874365 34-35 22.091417868333785 26.07890187687376 30.43188622322267 21.39779403156978 36-37 22.272604144902548 26.018481868282432 30.26089640683419 21.448017579980828 38-39 22.06214572103726 26.263302933877377 30.297955268646948 21.376596076438418 40-41 21.953446106785595 26.215299878918195 30.256277132180227 21.574976882115983 42-43 21.882328052104995 26.416579567783057 29.82417137070632 21.876921009405628 44-45 22.09331839298318 26.452294165310303 29.622866400144677 21.83152104156184 46-47 22.420999942517255 26.735959183577044 29.08973318820034 21.75330768570535 48-49 22.458773235537528 26.941346401938347 28.58923582940294 22.01064453312118 50-51 22.40503209234532 27.072825856143663 28.475660187826772 22.046481863684242 52-53 22.505909048554596 27.23278042224926 28.226602989368306 22.034707539827842 54-55 22.299300286678253 27.191519027278265 28.161584483992268 22.34759620205121 56-57 22.369460950691593 27.536847376935025 27.938885574369714 22.154806098003668 58-59 22.583534960242446 27.04618721721268 28.087359003339245 22.282918819205626 60-61 22.42540116510726 27.096460738657214 28.441840968161397 22.03629712807413 62-63 22.374605167767935 27.068285699736943 28.626957625058864 21.930151507436257 64-65 22.330998941426195 27.127390813742192 28.41605283997267 22.125557404858938 66-67 22.233397946635623 27.314628285402687 28.156237327240348 22.295736440721342 68-69 22.164059951089797 27.16361505039977 28.456504329972997 22.215820668537436 70-71 22.224517458024305 27.189628707257867 28.261961707417456 22.32389212730037 72-73 22.297180423153073 27.261972787740756 27.922527715825417 22.518319073280754 74-75 22.27339840381761 27.634875574682095 27.666688978046917 22.425037043453383 76-77 22.376180034573576 27.410294274361746 27.82989128694441 22.383634404120272 78-79 22.16846945926721 27.48335227556085 27.698138762096995 22.65003950307494 80-81 22.14755746409888 27.69581890770309 27.61240179853377 22.544221829664256 82-83 22.114014176965867 27.489591431875652 27.692434489409273 22.703959901749208 84-85 22.096497822425864 27.472922065104637 27.432709389397292 22.997870723072207 86-87 22.16699660299447 27.42049111954194 27.582467020803573 22.830045256660014 88-89 22.226191566749865 27.319671380270584 27.465901332711073 22.98823572026848 90-91 22.062919019598475 27.33778222713822 27.596641911120468 23.00265684214284 92-93 21.9439578397657 27.530876576113577 27.424126854471282 23.10103872964944 94-95 21.89765675567989 27.39895523336114 27.349766893464984 23.35362111749399 96-97 21.98354730170446 27.38467926792553 27.153199183900806 23.4785742464692 98-99 21.787423408213748 27.53878935631402 27.024878944030778 23.648908291441458 100-101 21.701184507512135 27.738400937169683 27.044474350019648 23.515940205298534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3278.0 1 2248.0 2 1323.0 3 1379.5 4 1168.0 5 1181.0 6 1940.5 7 3872.0 8 5990.5 9 6544.5 10 5526.0 11 4516.5 12 4230.0 13 4187.5 14 4255.0 15 4225.5 16 4259.0 17 4395.0 18 4448.5 19 4517.5 20 4838.5 21 5397.5 22 6052.0 23 6894.5 24 7769.0 25 9194.0 26 11332.5 27 13386.5 28 15016.0 29 17106.5 30 20044.0 31 23279.5 32 26744.0 33 30883.0 34 35281.5 35 38689.5 36 43322.5 37 47919.0 38 50117.5 39 50735.0 40 51008.0 41 52495.0 42 54185.0 43 57004.0 44 61164.0 45 63999.0 46 64829.0 47 67068.0 48 72899.0 49 72418.5 50 67532.0 51 65493.5 52 64811.0 53 65041.5 54 64113.0 55 61080.5 56 56589.0 57 52220.5 58 48809.0 59 44602.0 60 37890.5 61 30707.5 62 24435.5 63 18943.0 64 14095.5 65 10260.0 66 7395.0 67 5724.0 68 4238.0 69 2830.0 70 1873.5 71 1161.0 72 733.5 73 528.5 74 374.5 75 233.5 76 137.0 77 72.5 78 34.5 79 13.0 80 8.5 81 5.5 82 3.0 83 4.5 84 3.5 85 2.0 86 2.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01937086170937093 2 0.0027297691297758493 3 2.099822407519884E-4 4 0.0 5 0.002099822407519884 6 0.0 7 2.099822407519884E-4 8 0.0 9 1.574866805639913E-4 10-11 2.624778009399855E-5 12-13 0.0051708126785177146 14-15 0.003464706972407809 16-17 0.0 18-19 4.987078217859724E-4 20-21 0.0012598934445119303 22-23 0.0013911323449819232 24-25 7.086900625379609E-4 26-27 2.8872558103398404E-4 28-29 1.8373446065798985E-4 30-31 0.0 32-33 1.574866805639913E-4 34-35 0.0 36-37 0.001522371245451916 38-39 0.009134227472711495 40-41 6.561945023499637E-4 42-43 0.0 44-45 7.61185622725958E-4 46-47 1.8373446065798985E-4 48-49 7.874334028199564E-5 50-51 0.0015486190255459143 52-53 2.8872558103398404E-4 54-55 0.0 56-57 2.3623002084598697E-4 58-59 1.049911203759942E-4 60-61 0.0017848490463919014 62-63 0.0 64-65 7.874334028199564E-5 66-67 2.624778009399855E-5 68-69 1.8373446065798985E-4 70-71 5.774511620679681E-4 72-73 4.987078217859724E-4 74-75 0.0034384591923138103 76-77 0.0 78-79 4.199644815039768E-4 80-81 0.0019948312871438897 82-83 1.049911203759942E-4 84-85 0.002677273569587852 86-87 5.24955601879971E-4 88-89 0.002441043548741865 90-91 0.001574866805639913 92-93 0.0 94-95 0.0 96-97 3.9371670140997823E-4 98-99 9.974156435719448E-4 100-101 0.0020210790672378883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1904923.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.9784345773931 #Duplication Level Percentage of deduplicated Percentage of total 1 65.69999897108491 26.922831095713967 2 15.760000746050634 12.91640319023405 3 6.849548291548353 8.420512996497267 4 3.5241202395090134 5.776517227103481 5 2.0872936826461586 4.2767013809060765 6 1.3443268192531734 3.3053045168040676 7 0.9073281433713835 2.602662087436025 8 0.6657928130476253 2.18265177852565 9 0.4872561423647568 1.797029455709441 >10 2.414805619124336 17.803053902690817 >50 0.17771321118826702 5.024500597711326 >100 0.07808521281249663 5.595023665451416 >500 0.0020579906200075115 0.5253433394420188 >1k 0.0012862441375046947 1.011524433353244 >5k 1.2862441375046947E-4 0.44844197170389244 >10k+ 2.5724882750093893E-4 1.3914983607172966 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15326 0.8045469554412435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11074 0.5813358335218799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8508 0.4466322260794793 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3347 0.1757026399492263 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3226 0.16935067716647864 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2385 0.12520191104837308 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2289 0.12016233727032537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.049911203759942E-4 2 5.24955601879971E-5 0.0 0.0 5.24955601879971E-5 1.049911203759942E-4 3 5.24955601879971E-5 0.0 0.0 5.24955601879971E-5 1.049911203759942E-4 4 5.24955601879971E-5 0.0 0.0 1.049911203759942E-4 1.049911203759942E-4 5 5.24955601879971E-5 0.0 0.0 1.049911203759942E-4 1.049911203759942E-4 6 1.049911203759942E-4 0.0 0.0 1.049911203759942E-4 1.049911203759942E-4 7 1.049911203759942E-4 0.0 0.0 1.574866805639913E-4 1.049911203759942E-4 8 1.049911203759942E-4 0.0 0.0 1.574866805639913E-4 1.049911203759942E-4 9 1.049911203759942E-4 0.0 0.0 2.624778009399855E-4 1.049911203759942E-4 10-11 1.049911203759942E-4 0.0 0.0 2.624778009399855E-4 1.049911203759942E-4 12-13 1.8373446065798985E-4 0.0 0.0 4.199644815039768E-4 1.049911203759942E-4 14-15 2.099822407519884E-4 0.0 0.0 5.24955601879971E-4 1.3123890046999275E-4 16-17 2.624778009399855E-4 0.0 0.0 6.299467222559653E-4 1.574866805639913E-4 18-19 3.937167014099783E-4 0.0 0.0 9.186723032899493E-4 1.574866805639913E-4 20-21 5.24955601879971E-4 0.0 0.0 0.0010236634236659434 1.8373446065798985E-4 22-23 5.24955601879971E-4 0.0 0.0 0.0014173801250759216 2.099822407519884E-4 24-25 6.299467222559651E-4 0.0 0.0 0.00217856574780188 2.099822407519884E-4 26-27 6.299467222559651E-4 0.0 0.0 0.00307099027099783 2.099822407519884E-4 28-29 8.924245231959507E-4 0.0 0.0 0.005407042699363701 2.099822407519884E-4 30-31 9.449200833839478E-4 0.0 0.0 0.011155306539949384 2.099822407519884E-4 32-33 9.449200833839478E-4 0.0 0.0 0.021496931896984813 2.099822407519884E-4 34-35 0.0010761589838539404 0.0 0.0 0.0343845919231381 2.099822407519884E-4 36-37 0.0013648845648879247 0.0 0.0 0.047718464210889364 2.099822407519884E-4 38-39 0.001522371245451916 0.0 0.0 0.06388709674879246 2.099822407519884E-4 40-41 0.001522371245451916 0.0 0.0 0.08525278974530728 2.099822407519884E-4 42-43 0.001574866805639913 0.0 0.0 0.11268171994353578 2.099822407519884E-4 44-45 0.0017323534862039042 0.0 0.0 0.14520271947999996 2.099822407519884E-4 46-47 0.0018898401667678956 0.0 0.0 0.18528307968353575 2.099822407519884E-4 48-49 0.0019423357269558927 0.0 0.0 0.23048175700540124 2.099822407519884E-4 50-51 0.0019948312871438897 0.0 0.0 0.28512963516110623 2.624778009399855E-4 52-53 0.0020210790672378883 0.0 0.0 0.3537938278870064 3.149733611279826E-4 54-55 0.002099822407519884 0.0 0.0 0.4276025855113304 3.149733611279826E-4 56-57 0.002099822407519884 0.0 0.0 0.5166875511503615 3.149733611279826E-4 58-59 0.002099822407519884 0.0 0.0 0.6213374503851337 3.149733611279826E-4 60-61 0.002099822407519884 0.0 0.0 0.7450169901880548 3.674689213159797E-4 62-63 0.00217856574780188 0.0 0.0 0.8673053976459941 3.674689213159797E-4 64-65 0.0022048135278958784 0.0 0.0 0.9999879260211568 4.199644815039768E-4 66-67 0.0022048135278958784 0.0 0.0 1.1640365516086477 4.199644815039768E-4 68-69 0.0022048135278958784 0.0 0.0 1.3523643737830873 4.199644815039768E-4 70-71 0.002257309088083875 0.0 0.0 1.5515325291363484 4.199644815039768E-4 72-73 0.002257309088083875 0.0 0.0 1.755005320425025 4.199644815039768E-4 74-75 0.002257309088083875 0.0 0.0 1.9928627036368398 4.4621226159797535E-4 76-77 0.002257309088083875 0.0 0.0 2.2291189722629206 4.724600416919739E-4 78-79 0.002257309088083875 0.0 0.0 2.480835183364367 4.987078217859724E-4 80-81 0.002546034669117859 0.0 0.0 2.7540745741428916 5.24955601879971E-4 82-83 0.002624778009399855 0.0 0.0 3.07781469382227 5.774511620679681E-4 84-85 0.002756016909869848 0.0 0.0 3.427855089155835 5.774511620679681E-4 86-87 0.0028872558103398404 0.0 0.0 3.800888539851742 6.036989421619667E-4 88-89 0.0028872558103398404 0.0 0.0 4.219225658989891 6.299467222559651E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14560 0.0 34.16124 1 GGTATCA 9305 0.0 32.82789 1 ATCAACG 18380 0.0 26.953016 3 TATCAAC 18485 0.0 26.825607 2 TCAACGC 18485 0.0 26.774218 4 CAACGCA 18720 0.0 26.463484 5 AACGCAG 19150 0.0 25.869944 6 GTACATG 10065 0.0 23.92999 1 ACGCAGA 21060 0.0 23.365837 7 GAGTACT 11290 0.0 23.33068 12-13 AGTACTT 11475 0.0 23.244322 12-13 CGCAGAG 21085 0.0 23.182875 8 TACATGG 10450 0.0 22.635057 2 GCAGAGT 21420 0.0 22.37676 9 ACATGGG 10335 0.0 22.197557 3 GTACTTT 12115 0.0 21.584517 14-15 AGAGTAC 18265 0.0 20.676308 10-11 TACTTTT 13260 0.0 20.09684 14-15 TATAGGG 355 3.6379788E-12 20.069263 2 CATGGGG 4235 0.0 19.851273 4 >>END_MODULE