##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936799_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 239555 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968829705078164 33.0 31.0 34.0 30.0 34.0 2 32.06220700882887 33.0 31.0 34.0 30.0 34.0 3 32.02341424725011 34.0 31.0 34.0 30.0 34.0 4 35.62369393249984 37.0 35.0 37.0 33.0 37.0 5 35.65435077539605 37.0 35.0 37.0 35.0 37.0 6 35.75677819289933 37.0 35.0 37.0 35.0 37.0 7 35.71079710296174 37.0 35.0 37.0 35.0 37.0 8 35.68656884640271 37.0 35.0 37.0 35.0 37.0 9 37.32750725303166 39.0 37.0 39.0 35.0 39.0 10-11 37.23425100707562 39.0 37.0 39.0 34.0 39.0 12-13 37.143480620316836 39.0 37.0 39.0 34.0 39.0 14-15 38.281175512930226 40.0 38.0 41.0 33.5 41.0 16-17 38.219884786374735 40.0 38.0 41.0 33.5 41.0 18-19 38.240902924171905 40.0 38.0 41.0 34.0 41.0 20-21 38.2253073406942 40.0 38.0 41.0 34.0 41.0 22-23 38.10838429588195 40.0 38.0 41.0 34.0 41.0 24-25 37.92832126234059 40.0 38.0 41.0 33.0 41.0 26-27 37.82854250589635 40.0 37.0 41.0 33.0 41.0 28-29 37.826918661685205 40.0 37.0 41.0 33.0 41.0 30-31 37.597434827075205 40.0 37.0 41.0 33.0 41.0 32-33 37.28598234225961 40.0 36.0 41.0 33.0 41.0 34-35 37.042359792114546 39.0 35.0 41.0 32.5 41.0 36-37 36.837281626348855 39.0 35.0 41.0 31.5 41.0 38-39 36.617613909123165 39.0 35.0 41.0 31.0 41.0 40-41 36.350641815032034 39.0 35.0 41.0 30.5 41.0 42-43 36.04902840683768 39.0 35.0 41.0 30.0 41.0 44-45 35.64069211663292 39.0 35.0 40.5 29.0 41.0 46-47 35.054329903362486 38.5 35.0 40.0 25.0 41.0 48-49 34.51208490743253 38.0 35.0 40.0 22.5 41.0 50-51 33.77334015153096 37.5 33.5 39.5 20.0 40.5 52-53 33.99908997933669 38.0 34.5 40.0 20.0 40.5 54-55 34.26019494479347 39.0 35.0 40.5 18.5 41.0 56-57 34.11311181148379 38.5 35.0 41.0 15.5 41.0 58-59 33.88262194485608 38.0 34.0 40.5 11.0 41.0 60-61 33.56748972052347 38.0 34.0 40.0 7.5 41.0 62-63 33.26772140009601 38.0 34.0 40.0 4.5 41.0 64-65 32.958103567030534 37.0 33.5 40.0 2.0 41.0 66-67 32.553175679906495 36.5 33.0 39.5 2.0 41.0 68-69 32.10943207196677 36.0 33.0 39.0 2.0 41.0 70-71 31.71982634468076 35.5 32.0 39.0 2.0 40.5 72-73 31.33817912379203 35.0 32.0 38.5 2.0 40.0 74-75 30.924969213750494 35.0 31.5 37.0 2.0 39.5 76-77 30.49194130784162 35.0 31.0 37.0 2.0 39.0 78-79 30.063286092964038 35.0 31.0 36.0 2.0 39.0 80-81 29.63830226879005 35.0 30.0 36.0 2.0 38.5 82-83 29.21349585690134 35.0 30.0 35.5 2.0 37.0 84-85 28.854131619043642 34.0 29.0 35.0 2.0 37.0 86-87 28.5467721400096 34.0 29.0 35.0 2.0 36.0 88-89 28.1361065308593 34.0 29.0 35.0 2.0 36.0 90-91 27.756738536035563 34.0 28.5 35.0 2.0 36.0 92-93 27.397057043267726 34.0 27.0 35.0 2.0 35.0 94-95 26.99410365051867 34.0 26.0 35.0 2.0 35.0 96-97 26.560904593934588 34.0 25.0 35.0 2.0 35.0 98-99 26.02781824633174 34.0 24.0 35.0 2.0 35.0 100-101 24.208415603932288 31.5 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5131254502815175 1101 2 0.38226302094988895 1101 3 0.62333800251292 1101 4 0.4544628023373676 1101 5 0.31968080246380026 1101 6 0.27979123994394683 1101 7 0.3326886006076748 1101 8 0.061604937095744106 1101 9 0.19736648075960517 1101 10-11 0.24466681434300597 1101 12-13 0.5314675816292933 1101 14-15 0.7107345171090316 1101 16-17 0.6848657347823348 1101 18-19 0.727766611603542 1101 20-21 0.4591405745944286 1101 22-23 0.6028850277236515 1101 24-25 0.6421140306140813 1101 26-27 0.5578468267934014 1101 28-29 0.723085908839117 1101 30-31 0.5855470084698666 1101 32-33 0.7355641162762652 1101 34-35 0.8421952372337316 1101 36-37 1.1413027550207104 1101 38-39 1.2709553039362689 1101 40-41 1.03603959870793 1101 42-43 1.0925585191053102 1101 44-45 1.351862917278666 1101 46-47 1.2798086707667053 1101 48-49 1.2665225657056425 1101 50-51 1.339722920370626 1101 52-53 1.3381896047638904 1101 54-55 1.2866304147816692 1101 56-57 0.9364364216068708 1101 58-59 1.3504300505282245 1101 60-61 1.4139333049846172 1101 62-63 0.9808937953122339 1101 64-65 0.8217946930015714 1101 66-67 0.9067119308209399 1101 68-69 0.9940631712143251 1101 70-71 0.8694069712851302 1101 72-73 1.0495663190857591 1101 74-75 1.0087253969274244 1101 76-77 0.8209590368619395 1101 78-79 1.011387991503696 1101 80-81 1.096034764028385 1101 82-83 1.0956502363963843 1101 84-85 1.1100519969108795 1101 86-87 0.8746118442064628 1101 88-89 1.1198669869262545 1101 90-91 0.9635483695192057 1101 92-93 1.0189734404268727 1101 94-95 1.1453665524877437 1101 96-97 0.8726335775179663 1101 98-99 0.9881744792057034 1101 100-101 1.1005371223731153 1102 1 0.36683492238494964 1102 2 0.5345781046307927 1102 3 0.3993676618644777 1102 4 0.4288452413732031 1102 5 0.3383938822613004 1102 6 0.11369065860657912 1102 7 0.1856178722054409 1102 8 0.3169592830472112 1102 9 0.3353622453854328 1102 10-11 0.4724794958276206 1102 12-13 0.41578085853007707 1102 14-15 0.6538004117878344 1102 16-17 0.5881384705876727 1102 18-19 0.6047325115618918 1102 20-21 0.5188288463623252 1102 22-23 0.4210895106501624 1102 24-25 0.7385215885865009 1102 26-27 0.5250666732170899 1102 28-29 0.5997907642673752 1102 30-31 0.5316281141254464 1102 32-33 0.6758641121699469 1102 34-35 0.4742691040365017 1102 36-37 0.610927730822759 1102 38-39 0.6750845004470634 1102 40-41 0.7489258126236606 1102 42-43 1.0421534601209856 1102 44-45 1.0263462544250075 1102 46-47 1.0627633428771759 1102 48-49 1.5374486578396969 1102 50-51 1.20886041751087 1102 52-53 1.590496475807896 1102 54-55 1.4604098116221493 1102 56-57 1.5913619505886842 1102 58-59 1.6783371773372053 1102 60-61 1.5177146376609443 1102 62-63 1.8277222509258593 1102 64-65 1.9578810195477487 1102 66-67 1.5245192740915599 1102 68-69 1.6231100947018753 1102 70-71 1.8158869764473593 1102 72-73 1.7254194067439208 1102 74-75 1.9876423847668576 1102 76-77 1.671852747741923 1102 78-79 1.7275445947570702 1102 80-81 1.834529739654453 1102 82-83 1.6269139852304804 1102 84-85 1.8548971007127442 1102 86-87 1.855793173363086 1102 88-89 1.9016924214627409 1102 90-91 1.7855130669128698 1102 92-93 1.937416089823536 1102 94-95 1.9770074960024004 1102 96-97 1.8636407401105757 1102 98-99 1.3738545735337055 1102 100-101 1.3393488711368207 1103 1 0.3288585900220262 1103 2 0.32558127638072065 1103 3 0.5247918101486668 1103 4 0.4392918263689367 1103 5 0.36387208706386076 1103 6 0.3478465628158247 1103 7 0.3018068225320576 1103 8 0.18524373756483925 1103 9 0.39417964507151027 1103 10-11 0.43442500946223817 1103 12-13 0.2815839234695332 1103 14-15 0.5025105451055296 1103 16-17 0.5469122342890458 1103 18-19 0.6551320738994804 1103 20-21 0.5549783289176986 1103 22-23 0.2708547230011007 1103 24-25 0.4752891390446763 1103 26-27 0.6729600972090637 1103 28-29 0.7158820220909163 1103 30-31 0.8937318896150472 1103 32-33 0.8743459706721239 1103 34-35 1.051182411709263 1103 36-37 0.9048030621631611 1103 38-39 1.0674501569732584 1103 40-41 0.9660018108782253 1103 42-43 1.0770524786369648 1103 44-45 1.4276247236486128 1103 46-47 1.6734163846960826 1103 48-49 1.9965350375509203 1103 50-51 1.730408127272959 1103 52-53 1.6273688828860386 1103 54-55 1.9120096891808842 1103 56-57 1.9642933118360233 1103 58-59 1.7819465896842317 1103 60-61 1.5957709476197195 1103 62-63 1.8485340104980992 1103 64-65 1.7567549506289382 1103 66-67 1.9000199481698345 1103 68-69 2.03149732173274 1103 70-71 1.6729661008620127 1103 72-73 1.7072420883380026 1103 74-75 1.772393236319843 1103 76-77 1.5123274030502074 1103 78-79 1.8560949031397413 1103 80-81 1.7827494566067088 1103 82-83 1.4560400280979486 1103 84-85 1.8312131815500372 1103 86-87 1.7202122611756216 1103 88-89 1.6769768694675378 1103 90-91 1.980552892889957 1103 92-93 2.1648474934042916 1103 94-95 2.1493500970978587 1103 96-97 1.8384849205681704 1103 98-99 1.7909331769216799 1103 100-101 2.043073560637481 1104 1 0.5134631740234958 1104 2 0.5220806501292152 1104 3 0.2518418863359564 1104 4 0.49938006002058444 1104 5 0.5550742506232069 1104 6 0.3463228171138226 1104 7 0.49175648307981135 1104 8 0.5438744406418436 1104 9 0.5827092703577179 1104 10-11 0.6185269967138325 1104 12-13 0.5672662982790726 1104 14-15 0.6533214809725862 1104 16-17 0.5371115976664953 1104 18-19 0.5047000800263675 1104 20-21 0.6644766097176955 1104 22-23 0.7643169763896438 1104 24-25 0.6407631356461678 1104 26-27 0.748154155398602 1104 28-29 0.6430453819900777 1104 30-31 0.718441300938629 1104 32-33 0.6237775576811941 1104 34-35 0.9109220187264739 1104 36-37 0.8089825929133099 1104 38-39 0.4916577693195805 1104 40-41 0.5520814764519386 1104 42-43 0.7848921901623811 1104 44-45 1.0536528531111529 1104 46-47 0.8541078940021052 1104 48-49 0.7486232073807528 1104 50-51 0.7860248453959571 1104 52-53 0.9535222626497415 1104 54-55 1.0709938055280404 1104 56-57 1.18702743815534 1104 58-59 0.9853506854488572 1104 60-61 1.0772227342314977 1104 62-63 1.1701879526914993 1104 64-65 1.0789713225186262 1104 66-67 0.8562222313950656 1104 68-69 0.9006825565571113 1104 70-71 0.8884883627069442 1104 72-73 0.7329503109803888 1104 74-75 0.4362836542729127 1104 76-77 0.7047820696211424 1104 78-79 0.8388111593523959 1104 80-81 0.7763792422452092 1104 82-83 0.9077069739850323 1104 84-85 0.8398730033276323 1104 86-87 1.1665740191473617 1104 88-89 0.7218595570039312 1104 90-91 0.7122819054867868 1104 92-93 1.1017157572117426 1104 94-95 1.2399292002418782 1104 96-97 1.2059669108512985 1104 98-99 1.1710518854873655 1104 100-101 1.2869606279455574 1105 1 0.577028655720337 1105 2 0.40783458167408426 1105 3 0.3195701802437121 1105 4 0.35916597253545035 1105 5 0.3059662818112585 1105 6 0.29907820233914606 1105 7 0.2287724735648098 1105 8 0.2919912636892903 1105 9 0.2173729415616279 1105 10-11 0.41666364267656775 1105 12-13 0.29671654276169335 1105 14-15 0.5230188511324982 1105 16-17 0.5988269406029261 1105 18-19 0.3685496901756977 1105 20-21 0.3651175506454152 1105 22-23 0.514977740232645 1105 24-25 0.3894085697680083 1105 26-27 0.19321164185294037 1105 28-29 0.23667561854353636 1105 30-31 0.06937297795726494 1105 32-33 0.7012521182732456 1105 34-35 0.5323043366860318 1105 36-37 0.40988123173979574 1105 38-39 0.38101286380881305 1105 40-41 0.6418792827159763 1105 42-43 0.3279409545340002 1105 44-45 0.9021727421028274 1105 46-47 0.7024956302251795 1105 48-49 0.8885729893286864 1105 50-51 1.0632483157276624 1105 52-53 0.8686471470160697 1105 54-55 1.2470873696882094 1105 56-57 1.4125130995799466 1105 58-59 1.175089022967022 1105 60-61 0.9735158490722498 1105 62-63 0.9176109737037876 1105 64-65 0.8978229149594839 1105 66-67 1.002614460812275 1105 68-69 1.3159726318841898 1105 70-71 0.7026485318624864 1105 72-73 0.5612508263761846 1105 74-75 0.8021816323355537 1105 76-77 0.6838358557979625 1105 78-79 1.0427889576872715 1105 80-81 1.0596156635482323 1105 82-83 1.0546608526687926 1105 84-85 0.9169511056138759 1105 86-87 0.888590670396205 1105 88-89 0.640618642506773 1105 90-91 0.43558704622948596 1105 92-93 0.4741552973201095 1105 94-95 0.47258414936306536 1105 96-97 0.5209199966184457 1105 98-99 0.6920311375026955 1105 100-101 1.1906542978279404 1106 1 0.4942129207306927 1106 2 0.3964474181130093 1106 3 0.5113403665791161 1106 4 0.3383649890986007 1106 5 0.5728205778775148 1106 6 0.46525962055860504 1106 7 0.612830444579302 1106 8 0.27558986617376036 1106 9 0.36883515688253965 1106 10-11 0.6542412824281172 1106 12-13 0.6714092187451328 1106 14-15 1.034519239331253 1106 16-17 1.0958992749714582 1106 18-19 1.31523706077612 1106 20-21 1.1340260651381655 1106 22-23 1.085856363421044 1106 24-25 1.0297100708032048 1106 26-27 1.0772210880227178 1106 28-29 1.0455039052930175 1106 30-31 0.9022210628433882 1106 32-33 0.9728941990185831 1106 34-35 0.6022609878247565 1106 36-37 0.6148178259426942 1106 38-39 0.814210327576923 1106 40-41 0.6245073883060428 1106 42-43 0.5717621243062538 1106 44-45 0.9000861737798473 1106 46-47 0.7475428610740309 1106 48-49 2.0672576425377898 1106 50-51 1.6271231863280775 1106 52-53 1.746914486560577 1106 54-55 1.9636214017793066 1106 56-57 2.3172911792900877 1106 58-59 2.092580611993938 1106 60-61 2.511801951556727 1106 62-63 2.3709462480309114 1106 64-65 2.0885093797628045 1106 66-67 2.170914797251193 1106 68-69 2.3843926629400904 1106 70-71 2.0926265339328864 1106 72-73 2.25352243610703 1106 74-75 1.8200064252962207 1106 76-77 2.059304929296932 1106 78-79 2.143332435948139 1106 80-81 1.9710901718298075 1106 82-83 1.5641231973892893 1106 84-85 1.547256488636652 1106 86-87 1.3965163950338848 1106 88-89 1.5530636476827588 1106 90-91 1.8379388837036075 1106 92-93 2.0159998884174186 1106 94-95 1.8607182073340773 1106 96-97 1.818943417943494 1106 98-99 2.0724497451631514 1106 100-101 2.5593137181177994 1107 1 -0.04182311530534477 1107 2 0.2117627334283192 1107 3 0.16787189811065062 1107 4 -0.05591992081131281 1107 5 -0.14747784554591448 1107 6 -0.2539126767386932 1107 7 0.180398012146874 1107 8 0.042340992299884306 1107 9 -0.08414907735169663 1107 10-11 -0.025938897752062928 1107 12-13 0.18597847550189073 1107 14-15 0.30908286545737695 1107 16-17 0.24743362181830975 1107 18-19 0.4999217454607958 1107 20-21 0.5559714480210545 1107 22-23 0.48070433639401955 1107 24-25 0.6258530888212306 1107 26-27 0.5535020564911832 1107 28-29 0.34442000889661273 1107 30-31 0.4510667835641158 1107 32-33 0.6349155954149808 1107 34-35 0.6523032175544827 1107 36-37 0.6943504836003527 1107 38-39 0.3734727149643149 1107 40-41 0.4439049108285644 1107 42-43 0.6634006378197697 1107 44-45 0.8013389890951643 1107 46-47 1.035479234947907 1107 48-49 1.2517311785738272 1107 50-51 1.3635392899316798 1107 52-53 1.27154849556959 1107 54-55 1.4957863792567778 1107 56-57 1.6142365621729766 1107 58-59 1.5070654092912363 1107 60-61 1.1157574695747385 1107 62-63 1.2060391534363077 1107 64-65 1.4533038617447787 1107 66-67 1.552038108062014 1107 68-69 1.4273262214986495 1107 70-71 1.225087119518463 1107 72-73 1.394640116287217 1107 74-75 1.4997643081791026 1107 76-77 1.3993246365041365 1107 78-79 1.7824090125247167 1107 80-81 1.9421628069649408 1107 82-83 1.7929379496415443 1107 84-85 1.8113330652132262 1107 86-87 1.8502044580969503 1107 88-89 1.7687871837187927 1107 90-91 1.6741579152351385 1107 92-93 1.574065766795794 1107 94-95 1.6864501893160622 1107 96-97 2.2132987107362894 1107 98-99 2.4466755334514403 1107 100-101 2.648840745144824 1108 1 0.03267445919222922 1108 2 0.20841323007882195 1108 3 0.05087028110903091 1108 4 -0.14173650662790038 1108 5 -0.018926216994280765 1108 6 0.03483761201160007 1108 7 -0.045289213540350204 1108 8 -0.09233214237325171 1108 9 0.12871763551501658 1108 10-11 0.11150623969307105 1108 12-13 -0.36137657185316385 1108 14-15 -0.04567573930122393 1108 16-17 0.18904831343299833 1108 18-19 -0.28519028965123283 1108 20-21 0.13890053095013144 1108 22-23 -0.08842248273280262 1108 24-25 0.22535643832458163 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-1.5136793988772617 2316 82-83 -1.8936577369541432 2316 84-85 -1.909182905302746 2316 86-87 -1.2254848675923746 2316 88-89 -1.4995720846404765 2316 90-91 -1.7210099799327558 2316 92-93 -2.1174439247138963 2316 94-95 -1.5228045199386493 2316 96-97 -1.55424155680398 2316 98-99 -1.8416127548368522 2316 100-101 -1.4185903222862422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 758.0 3 184.0 4 169.0 5 163.0 6 188.0 7 207.0 8 283.0 9 350.0 10 464.0 11 647.0 12 999.0 13 1301.0 14 1550.0 15 2180.0 16 2976.0 17 4316.0 18 3696.0 19 1956.0 20 1670.0 21 1661.0 22 1643.0 23 1767.0 24 1882.0 25 2210.0 26 2332.0 27 2678.0 28 3134.0 29 3848.0 30 4768.0 31 5829.0 32 7599.0 33 10100.0 34 14092.0 35 21837.0 36 30294.0 37 45359.0 38 45218.0 39 9247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55149945702114 19.980786901679057 16.089299139587336 20.37841450171247 2 15.318661758499804 21.974771130953624 40.48360207078958 22.222965039756986 3 18.787385129490392 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31.93440829458643 19.91991030622407 48-49 19.721659602012902 28.058299054102026 31.794282850639995 20.425758493245077 50-51 19.567463970988896 28.601074346174965 31.727242170488257 20.104219512347882 52-53 19.77930660096671 29.20063890425832 30.51101901053357 20.509035484241405 54-55 19.483486217459486 28.179610484795457 31.19065865112559 21.14624464661946 56-57 20.061724528191572 28.92950958651248 30.23249015456189 20.776275730734053 58-59 20.572407096093805 28.316087333326372 30.384772008227962 20.72673356235186 60-61 19.93814553549427 27.942890077808823 31.639540080105917 20.47942430659099 62-63 19.859374934741115 27.854580233971372 31.64899282047855 20.637052010808958 64-65 19.5424042360389 28.571339087731605 31.298205613252655 20.588051062976838 66-67 19.659799304562114 28.284482128499377 30.67319640377165 21.382522163166854 68-69 19.503592750599836 27.98607587271159 31.201488469995674 21.308842906692895 70-71 20.027891615187567 28.08263934847191 30.811466460123967 21.078002576216555 72-73 19.651960436385497 27.614158730423817 31.469273066597637 21.264607766593045 74-75 19.317771389322093 28.901707554870782 30.668136429020294 21.112384626786838 76-77 19.469680676690633 28.165723222507356 30.572810851104258 21.791785249697757 78-79 19.241813849260797 28.253628137093358 30.7967178124876 21.707840201158245 80-81 19.54941223143257 28.171549078153387 30.412168792934246 21.8668698974798 82-83 19.28732382012476 28.218643689058837 30.80341573334057 21.690616757475834 84-85 19.508471638337372 27.950872224763124 30.324559919637956 22.21609621726155 86-87 19.351355641230207 28.122402436018994 30.143437744092694 22.3828041786581 88-89 19.487459082999624 27.88100594719592 29.872178110893167 22.75935685891129 90-91 19.317199293265556 27.480984582997444 30.551436650780456 22.650379472956548 92-93 19.789332091243477 27.764259142832675 30.39854792454722 22.047860841376632 94-95 19.385725982988824 27.91220359941848 30.15724479053523 22.544825627057467 96-97 19.349602667268307 27.72534322696013 30.05711396889858 22.86794013687298 98-99 19.937769567688658 27.815415092447086 29.131281554047277 23.11553378581698 100-101 20.016799769316602 27.31780399433321 29.623175323562723 23.042220912787467 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1326.0 1 852.5 2 385.0 3 362.5 4 298.0 5 317.0 6 501.0 7 932.5 8 1408.5 9 1514.5 10 1147.0 11 806.0 12 713.5 13 657.5 14 698.5 15 704.0 16 692.0 17 721.5 18 756.5 19 829.5 20 897.0 21 976.5 22 1163.0 23 1405.5 24 1554.5 25 1784.5 26 2245.0 27 2889.5 28 3253.5 29 3656.0 30 4125.5 31 4594.0 32 5060.5 33 5351.5 34 6117.5 35 6876.5 36 7495.0 37 7744.0 38 7273.5 39 6914.0 40 6887.0 41 6893.0 42 6976.5 43 7005.5 44 7095.5 45 7173.0 46 7441.0 47 8658.0 48 11632.0 49 11442.0 50 8093.5 51 6657.5 52 5982.0 53 5433.0 54 5061.0 55 4781.5 56 4408.0 57 3898.5 58 3513.0 59 3130.5 60 2660.0 61 2235.0 62 1773.5 63 1295.5 64 878.0 65 649.5 66 475.0 67 312.5 68 218.5 69 150.0 70 104.5 71 67.0 72 50.0 73 52.0 74 42.0 75 32.0 76 27.5 77 14.5 78 4.5 79 2.0 80 2.5 81 2.5 82 1.5 83 0.5 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05635449061802091 2 0.09434159170127945 3 0.0647033040429129 4 0.12064035398968921 5 0.10269040512617145 6 0.09601135438625785 7 0.10561248982488362 8 0.09141950700256726 9 0.10644737116737285 10-11 0.10039448143432615 12-13 0.08724510029012127 14-15 0.08974974431758886 16-17 0.10289912546179374 18-19 0.08474045626265367 20-21 0.07117363444720419 22-23 0.06908643109098118 24-25 0.062407380351067604 26-27 0.053014965248064115 28-29 0.0665817870635136 30-31 0.045918473836905935 32-33 0.049257999206862725 34-35 0.04341382980943834 36-37 0.048423117864373526 38-39 0.04696207551501743 40-41 0.05969401598797771 42-43 0.05677193128926551 44-45 0.04362255014506063 46-47 0.03068188933647805 48-49 0.04174406712445994 50-51 0.025255160610298262 52-53 0.03443885537767945 54-55 0.043205109473816034 56-57 0.04341382980943834 58-59 0.053223685583686425 60-61 0.050927761891841124 62-63 0.051762643234330316 64-65 0.03589989772703554 66-67 0.05739809229613241 68-69 0.04779695685750663 70-71 0.023585397925319863 72-73 0.017532508192273172 74-75 0.025255160610298262 76-77 0.039865584103859236 78-79 0.05906785498111081 80-81 0.03756966041201394 82-83 0.05572832961115401 84-85 0.05781553296737701 86-87 0.0617812193442007 88-89 0.08286197324205298 90-91 0.0601114566592223 92-93 0.07242595646093798 94-95 0.07639164283776168 96-97 0.08724510029012127 98-99 0.11813570996222161 100-101 0.11103921855106344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 239555.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.284602831407966 #Duplication Level Percentage of deduplicated Percentage of total 1 82.98261334691554 52.51521727571844 2 11.053138120872823 13.989869120402634 3 2.9636779086130915 5.626655381003919 4 1.1341752550916457 2.871033222387424 5 0.5532030809023452 1.7504618630008084 6 0.2986239864483974 1.133898022699115 7 0.16849498603854235 0.7464196789372949 8 0.11488078257694213 0.5816147758674493 9 0.08514376618357104 0.48494604838478017 >10 0.5325460496320532 6.45511917016452 >50 0.06401025303683308 2.755422369165633 >100 0.04355336804568023 5.666197439351214 >500 0.0019796985475309197 0.9438094201749516 >1k 0.003959397095061839 4.479336212741828 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2978 1.243138318966417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2374 0.991004153534679 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1515 0.632422616935568 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1417 0.5915134311535973 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1389 0.5798250923587485 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1053 0.43956502682056314 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 989 0.41284882386090876 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 697 0.29095614785748575 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 574 0.23961094529440005 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 477 0.19911920018367388 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 473 0.19744943749869548 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 438 0.18283901400513453 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 391 0.16321930245663835 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 366 0.15278328567552335 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 362 0.15111352299054498 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 343 0.1431821502368976 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 330 0.1377554215107178 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 327 0.136503099496984 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 326 0.1360856588257394 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 306 0.1277368454008474 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 301 0.12564964204462442 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 301 0.12564964204462442 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 301 0.12564964204462442 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 291 0.12147523533217841 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.10811713385235124 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 254 0.10602993049612823 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 252 0.10519504915363904 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 243 0.10143808311243764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.174406712445994E-4 0.0 20-21 0.0 0.0 0.0 4.174406712445994E-4 0.0 22-23 0.0 0.0 0.0 4.174406712445994E-4 0.0 24-25 0.0 0.0 0.0 0.002087203356222997 0.0 26-27 0.0 0.0 0.0 0.005218008390557492 0.0 28-29 0.0 0.0 0.0 0.011270898123604184 0.0 30-31 0.0 0.0 0.0 0.020245872555363066 0.0 32-33 0.0 0.0 0.0 0.03235165202145645 0.0 34-35 0.0 0.0 0.0 0.041535346788837635 0.0 36-37 0.0 0.0 0.0 0.054267287261797914 0.0 38-39 0.0 0.0 0.0 0.06783410907724739 0.0 40-41 0.0 0.0 0.0 0.08974974431758886 0.0 42-43 0.0 0.0 0.0 0.1346246164763833 0.0 44-45 0.0 0.0 0.0 0.19348375112187183 0.0 46-47 0.0 0.0 0.0 0.24837719939053662 0.0 48-49 0.0 0.0 0.0 0.3600425789484669 0.0 50-51 0.0 0.0 0.0 0.4681597128008182 0.0 52-53 0.0 0.0 0.0 0.5833733380643276 0.0 54-55 0.0 0.0 0.0 0.7589071403226817 0.0 56-57 0.0 0.0 0.0 0.9482164847321074 0.0 58-59 0.0 0.0 0.0 1.1761390912316587 0.0 60-61 0.0 0.0 0.0 1.4370395107595333 0.0 62-63 0.0 0.0 0.0 1.7709920477552128 0.0 64-65 0.0 0.0 0.0 2.0702970090375907 0.0 66-67 0.0 0.0 0.0 2.437436079397216 0.0 68-69 0.0 0.0 0.0 2.890359207697606 0.0 70-71 0.0 0.0 0.0 3.3196969380726764 0.0 72-73 0.0 0.0 0.0 3.774081108722423 0.0 74-75 0.0 0.0 0.0 4.3292772014777405 0.0 76-77 0.0 0.0 0.0 4.817891507169543 0.0 78-79 0.0 0.0 0.0 5.321533677026153 0.0 80-81 0.0 0.0 0.0 5.969192878462149 0.0 82-83 0.0 0.0 0.0 6.715994239318737 0.0 84-85 0.0 0.0 0.0 7.532716912608795 0.0 86-87 0.0 0.0 0.0 8.340464611467095 0.0 88-89 0.0 0.0 0.0 9.162196572812089 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 80 1.0674536E-5 35.657005 9 GTGTAGG 55 0.002566871 34.56926 1 TTTGCGT 60 0.0039314697 31.688488 3 TATGACA 105 6.851154E-5 27.161562 4 TAGGCAT 125 7.3077554E-6 26.6239 5 TTAGGCA 125 7.319646E-6 26.61833 4 GAGTACT 1815 0.0 24.999878 12-13 TTTAGGC 115 1.2716459E-4 24.799688 3 TCCAGAA 280 1.8189894E-12 23.766369 2 GTAGGAC 100 0.0016432282 23.766365 3 GCCGCCC 60 1.7068596E-4 23.753946 82-83 GTACATG 1305 0.0 23.311071 1 GTACTTT 1945 0.0 23.206793 14-15 CATGGGG 520 0.0 22.852276 4 TACATGG 1340 0.0 22.702202 2 GTATCAA 3935 0.0 21.984646 1 ATGGGAC 200 5.3033546E-7 21.394203 5 GCATTCC 180 5.1007137E-6 21.13008 8 GGCATTC 135 3.7609416E-4 21.13008 7 CTTTAGG 135 3.7662612E-4 21.12566 2 >>END_MODULE