##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 239555 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.925745653399012 31.0 31.0 34.0 30.0 34.0 2 31.923311974285653 31.0 31.0 34.0 30.0 34.0 3 32.06746676128655 33.0 31.0 34.0 30.0 34.0 4 35.87779424349314 37.0 35.0 37.0 35.0 37.0 5 35.80531401974494 37.0 35.0 37.0 35.0 37.0 6 35.872167143244766 37.0 35.0 37.0 35.0 37.0 7 35.810294086952894 37.0 35.0 37.0 35.0 37.0 8 35.80112291540565 37.0 35.0 37.0 33.0 37.0 9 37.418592807497234 39.0 37.0 39.0 34.0 39.0 10-11 37.362515914925595 39.0 37.0 39.0 34.0 39.0 12-13 37.27610152157125 39.0 37.0 39.0 34.0 39.0 14-15 38.33284214481017 40.0 38.0 41.0 33.5 41.0 16-17 38.277894429254246 40.0 38.0 41.0 33.5 41.0 18-19 38.37382855711632 40.0 38.0 41.0 33.5 41.0 20-21 38.46856254304856 40.0 38.0 41.0 34.0 41.0 22-23 38.36904886143057 40.0 38.0 41.0 34.0 41.0 24-25 38.22558076433387 40.0 37.5 41.0 34.0 41.0 26-27 38.0029847007994 40.0 37.0 41.0 33.0 41.0 28-29 37.791674145812024 40.0 37.0 41.0 33.0 41.0 30-31 37.53763436371605 39.0 36.0 41.0 33.0 41.0 32-33 37.35612281104548 39.0 35.5 41.0 33.0 41.0 34-35 37.171380267579465 39.0 35.0 41.0 32.5 41.0 36-37 36.903942727139906 39.0 35.0 41.0 31.5 41.0 38-39 36.66887771075535 39.0 35.0 41.0 31.0 41.0 40-41 36.392792886810966 39.0 35.0 41.0 30.5 41.0 42-43 36.044242449541855 39.0 35.0 41.0 30.0 41.0 44-45 35.48211058003381 39.0 35.0 40.0 28.0 41.0 46-47 34.96057481580431 38.0 35.0 40.0 25.0 41.0 48-49 34.43177140948843 38.0 34.5 40.0 22.5 41.0 50-51 34.03917680699631 38.0 34.0 40.0 19.5 41.0 52-53 33.75196301475653 38.0 33.5 40.0 17.5 41.0 54-55 33.50734278140719 38.0 33.0 40.0 14.0 41.0 56-57 33.19237753334308 38.0 33.0 40.0 10.0 41.0 58-59 32.83988854334078 37.0 33.0 40.0 7.5 41.0 60-61 32.461950282816055 37.0 32.0 40.0 4.0 41.0 62-63 32.086454050218116 36.0 32.0 39.0 2.0 41.0 64-65 31.653968817181855 36.0 31.0 39.0 2.0 40.0 66-67 31.236546930767467 35.0 31.0 39.0 2.0 40.0 68-69 30.76741249399929 35.0 30.5 38.0 2.0 40.0 70-71 30.2662248752896 35.0 29.5 37.5 2.0 39.5 72-73 29.92804366429421 34.5 29.0 37.0 2.0 39.0 74-75 29.51106009058463 34.0 29.0 36.5 2.0 39.0 76-77 28.14971092233516 32.5 27.0 35.0 2.0 37.5 78-79 28.849406190645155 34.0 29.0 36.0 2.0 37.5 80-81 28.654269374465155 34.0 29.0 35.0 2.0 37.0 82-83 28.305253490847612 34.0 28.5 35.0 2.0 37.0 84-85 27.918162843605852 34.0 27.5 35.0 2.0 36.0 86-87 27.555611446223207 34.0 27.0 35.0 2.0 36.0 88-89 27.157435661956544 33.0 26.0 35.0 2.0 35.5 90-91 26.690499050322472 33.0 25.0 35.0 2.0 35.0 92-93 26.21733422387343 33.0 24.5 35.0 2.0 35.0 94-95 25.697345077330887 32.5 23.5 35.0 2.0 35.0 96-97 25.013760931727578 32.0 18.5 34.0 2.0 35.0 98-99 24.31078666694496 32.0 4.5 34.0 2.0 35.0 100-101 21.638842854459313 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.330391777797324 1101 2 0.0913307498493694 1101 3 0.2573579258629053 1101 4 0.28527499604956574 1101 5 0.2798454594459798 1101 6 0.29758686742555085 1101 7 0.37529131270541427 1101 8 0.23284111700105115 1101 9 0.478519954466357 1101 10-11 0.24099060492482494 1101 12-13 0.24969600802078418 1101 14-15 0.646182739139654 1101 16-17 0.41071603141942603 1101 18-19 0.42741074700181514 1101 20-21 0.1812508249469147 1101 22-23 0.07848279018305249 1101 24-25 0.36746839674944454 1101 26-27 0.48854366551106665 1101 28-29 0.6152690820409887 1101 30-31 0.597261221903473 1101 32-33 0.8067417857224584 1101 34-35 0.7687836628823135 1101 36-37 0.5806841776158436 1101 38-39 0.7007280092386807 1101 40-41 0.7304851475159353 1101 42-43 0.6958423688635591 1101 44-45 0.9711698662922856 1101 46-47 1.022984827248031 1101 48-49 1.1031350171073413 1101 50-51 1.509479488816332 1101 52-53 1.251804617598161 1101 54-55 1.0737423935645012 1101 56-57 1.1388584993609356 1101 58-59 1.461303207090971 1101 60-61 1.1610055561037598 1101 62-63 1.1430836559717328 1101 64-65 1.184302265802902 1101 66-67 1.0794003226988522 1101 68-69 1.2967273188157407 1101 70-71 1.4320569817857027 1101 72-73 1.2490649487671526 1101 74-75 1.1803266275173314 1101 76-77 0.9476220344685657 1101 78-79 0.98800191861908 1101 80-81 1.069871028757703 1101 82-83 0.8631293068012837 1101 84-85 1.0217824304928378 1101 86-87 0.9758009424078651 1101 88-89 1.0723930210097947 1101 90-91 1.2657335691755414 1101 92-93 1.1535088201705435 1101 94-95 1.052719618167302 1101 96-97 1.4182053501310037 1101 98-99 1.47197032166698 1101 100-101 1.4070891995840782 1102 1 0.3605673522419437 1102 2 0.18788789513678594 1102 3 0.41690013001732495 1102 4 0.19554020555980145 1102 5 0.26884639216688555 1102 6 0.22570869602781585 1102 7 0.5467066433520387 1102 8 0.5054420004461377 1102 9 0.5225338220903808 1102 10-11 0.6550130958176013 1102 12-13 0.48407879902677564 1102 14-15 0.5168425071913205 1102 16-17 0.654695875262 1102 18-19 0.6778521762352838 1102 20-21 0.5393247269466883 1102 22-23 0.5352285914724391 1102 24-25 0.8194420693820064 1102 26-27 0.7653887223835767 1102 28-29 0.7758671966541968 1102 30-31 0.8293298091539398 1102 32-33 0.8173214219026477 1102 34-35 1.0216126229864884 1102 36-37 0.919690096580581 1102 38-39 1.0622952150063085 1102 40-41 1.098546202605128 1102 42-43 1.0952962285096035 1102 44-45 1.2330532585806253 1102 46-47 1.2201262429893092 1102 48-49 1.4502157179972457 1102 50-51 1.457115129314026 1102 52-53 1.6802021561535554 1102 54-55 1.3161941003264346 1102 56-57 1.2994536808895418 1102 58-59 1.3849608809202394 1102 60-61 1.5964688954792479 1102 62-63 1.5733231716607996 1102 64-65 1.5357561958429677 1102 66-67 1.7203643689477275 1102 68-69 1.5497351970809952 1102 70-71 1.2121489633190023 1102 72-73 1.556830583574655 1102 74-75 1.3762860101616816 1102 76-77 1.611710519765012 1102 78-79 1.82311856739393 1102 80-81 1.9529494770386222 1102 82-83 1.8042945626542526 1102 84-85 1.5736335001887944 1102 86-87 1.5599511398044577 1102 88-89 1.71501132697745 1102 90-91 2.0537859423342972 1102 92-93 1.7340395911611601 1102 94-95 1.7958254027967655 1102 96-97 2.0034466550016887 1102 98-99 2.2553561572147913 1102 100-101 1.9056813189723556 1103 1 -0.03775103413841663 1103 2 -0.29786528149777425 1103 3 -0.12290305215313779 1103 4 0.056816851190014006 1103 5 0.19110921105149714 1103 6 0.09094514444943513 1103 7 0.09541938180315412 1103 8 0.23468397175059152 1103 9 0.15345154546428574 1103 10-11 0.11182324020711576 1103 12-13 0.1823945802737228 1103 14-15 0.22095796670429024 1103 16-17 0.2080392383589853 1103 18-19 0.42399495057211567 1103 20-21 0.2880433269479852 1103 22-23 0.3379157865904219 1103 24-25 0.22771857824271535 1103 26-27 0.3516512026008485 1103 28-29 0.2950963376765827 1103 30-31 0.3546093725335808 1103 32-33 0.5994020117048535 1103 34-35 0.6173903530034934 1103 36-37 0.5987962753057658 1103 38-39 0.4626553747009794 1103 40-41 0.5609052811694397 1103 42-43 0.8465190130064499 1103 44-45 1.031621086485508 1103 46-47 1.3715170585914578 1103 48-49 1.3621491828898087 1103 50-51 1.2450019102157839 1103 52-53 1.6812083889352536 1103 54-55 1.7937866965372677 1103 56-57 1.7684167726618796 1103 58-59 1.7610184022660462 1103 60-61 1.7421935459029072 1103 62-63 1.9904896983015483 1103 64-65 1.5137916647344163 1103 66-67 1.6143028747734576 1103 68-69 1.7484873381750248 1103 70-71 2.2552639213437864 1103 72-73 2.672456173800196 1103 74-75 2.266521970120781 1103 76-77 1.9527876121756123 1103 78-79 1.9203095822887803 1103 80-81 2.287979403508732 1103 82-83 2.299764886928237 1103 84-85 2.4458810139145903 1103 86-87 2.4737440186722672 1103 88-89 2.746403184632957 1103 90-91 2.798486124042352 1103 92-93 2.5316011862843517 1103 94-95 2.562799475206454 1103 96-97 3.0007242905825997 1103 98-99 2.867979331179086 1103 100-101 2.295038046403757 1104 1 0.18922325365007708 1104 2 0.16394135437486668 1104 3 0.13543965500846866 1104 4 0.13431248338492452 1104 5 0.17244930949664194 1104 6 0.27191986303514426 1104 7 0.2986280232761658 1104 8 0.29160504810541 1104 9 0.1857134701705121 1104 10-11 0.25770793015279025 1104 12-13 0.10211073277593385 1104 14-15 0.3883306621386424 1104 16-17 0.4022729378699452 1104 18-19 0.3532803856374116 1104 20-21 0.26872127411948554 1104 22-23 0.290404438274912 1104 24-25 0.21954539776261583 1104 26-27 0.04545011603454441 1104 28-29 0.14380673823821866 1104 30-31 0.38483298819864586 1104 32-33 0.41126728386498 1104 34-35 0.002319630460831945 1104 36-37 -0.16365894970601147 1104 38-39 0.01970808353791398 1104 40-41 0.5768208449154599 1104 42-43 0.068351656266465 1104 44-45 0.08008577308053333 1104 46-47 0.5611476100916661 1104 48-49 0.7841549428081223 1104 50-51 1.3078584148548345 1104 52-53 0.7845638205701277 1104 54-55 0.6870487089984749 1104 56-57 0.4878959866962944 1104 58-59 0.48713907335237394 1104 60-61 0.6704618208791686 1104 62-63 0.5174504239555198 1104 64-65 0.8404994964682224 1104 66-67 0.7420143044617689 1104 68-69 0.6591048939592739 1104 70-71 0.6986898760781735 1104 72-73 0.6119491095236533 1104 74-75 0.6276417237685976 1104 76-77 0.8154031894837672 1104 78-79 0.6641248500611603 1104 80-81 1.1516001743166342 1104 82-83 0.92881657461621 1104 84-85 1.2865578442044203 1104 86-87 1.3204480210974978 1104 88-89 1.1007618153360355 1104 90-91 1.1243961831573053 1104 92-93 1.3748867330378154 1104 94-95 1.562344744813707 1104 96-97 1.8211097743120277 1104 98-99 1.6485998387220278 1104 100-101 1.3214762309923884 1105 1 0.3024781763407063 1105 2 0.1581266325564492 1105 3 0.11370052457368018 1105 4 0.3421887883935142 1105 5 0.2980605160514145 1105 6 0.04065251798426317 1105 7 0.3116450709833103 1105 8 0.22440536527205523 1105 9 0.0950566709105658 1105 10-11 0.28384114014353656 1105 12-13 0.3374417754612793 1105 14-15 0.49167412718423265 1105 16-17 0.33209981774307806 1105 18-19 0.6457675007424726 1105 20-21 0.5308132524464355 1105 22-23 0.513874769978365 1105 24-25 0.6802299491039676 1105 26-27 0.5652399931324652 1105 28-29 0.6584096191685731 1105 30-31 0.5075698720363633 1105 32-33 0.835642862033346 1105 34-35 0.6188453341743241 1105 36-37 0.3925720533335024 1105 38-39 0.4359562664373371 1105 40-41 0.2072225893320052 1105 42-43 0.3942073454431565 1105 44-45 0.3715487042289425 1105 46-47 -0.16820351981450443 1105 48-49 -0.09069638532720603 1105 50-51 -0.20261469205675553 1105 52-53 -0.11037472839247187 1105 54-55 -0.31057254116672084 1105 56-57 -0.07523999850258889 1105 58-59 -0.22338028702822754 1105 60-61 -0.4346987447346784 1105 62-63 -0.21024879634314786 1105 64-65 -0.2906621263677778 1105 66-67 -0.31382024027194433 1105 68-69 -0.17405223568259487 1105 70-71 -0.022666011327668656 1105 72-73 -0.3665430273866157 1105 74-75 -0.45148474643095327 1105 76-77 -0.23950628586974432 1105 78-79 0.1031033091598772 1105 80-81 0.21139930210836155 1105 82-83 0.48296617155026667 1105 84-85 0.3412690904050315 1105 86-87 0.7273794338105937 1105 88-89 0.74646024271809 1105 90-91 0.7683105481632495 1105 92-93 0.6103168902996075 1105 94-95 0.8619020330388949 1105 96-97 1.1282328085395932 1105 98-99 1.1870562943847744 1105 100-101 1.039495260991064 1106 1 0.12235395273822292 1106 2 0.35403760564133435 1106 3 0.33633251215132276 1106 4 0.3621810244183621 1106 5 0.2618933317864034 1106 6 0.38864035442318823 1106 7 0.3181474001758602 1106 8 0.2503499822492756 1106 9 0.3603905218422412 1106 10-11 0.28437491343546384 1106 12-13 0.545111935916772 1106 14-15 0.5768675795651816 1106 16-17 0.5710846779915286 1106 18-19 0.8159117812807679 1106 20-21 0.47699919965140225 1106 22-23 0.6052632275352963 1106 24-25 0.6143655598700093 1106 26-27 0.7106366028633175 1106 28-29 0.6339045725847186 1106 30-31 1.0179541888065486 1106 32-33 0.5789787833584015 1106 34-35 0.6971967834496908 1106 36-37 1.0153399104763636 1106 38-39 1.0516071463545202 1106 40-41 1.1557215541353258 1106 42-43 1.3155970969959512 1106 44-45 1.2390111783490312 1106 46-47 1.3360019667279346 1106 48-49 1.6995630608425643 1106 50-51 1.6099338327879664 1106 52-53 1.956573382373577 1106 54-55 1.6133405023115444 1106 56-57 1.9044280915595166 1106 58-59 1.9667885794314017 1106 60-61 1.837460934354354 1106 62-63 1.9372990815036424 1106 64-65 2.5525055754955766 1106 66-67 2.275433124117285 1106 68-69 2.3470560717708224 1106 70-71 2.00643272055639 1106 72-73 2.312691574062658 1106 74-75 2.0416409005669784 1106 76-77 2.080822389078495 1106 78-79 2.3911306124725087 1106 80-81 2.296673629230515 1106 82-83 2.5934410680305966 1106 84-85 2.541045876119316 1106 86-87 2.6942369648664943 1106 88-89 2.8673841557615667 1106 90-91 2.637519916693268 1106 92-93 2.6302929513762123 1106 94-95 2.4140338912169526 1106 96-97 2.797988890320134 1106 98-99 3.2679957353785625 1106 100-101 2.6594163272436546 1107 1 0.24397557435984396 1107 2 0.1817403083440361 1107 3 0.01960953917834729 1107 4 0.17045805144538662 1107 5 0.18390909755217422 1107 6 0.1734094985673309 1107 7 0.21721834612180402 1107 8 0.34804142369071656 1107 9 0.43443331463503654 1107 10-11 0.1578968728949235 1107 12-13 0.049405291772622206 1107 14-15 0.549910935421039 1107 16-17 0.4278555113248643 1107 18-19 0.5352952272267189 1107 20-21 0.5937644023541111 1107 22-23 0.27460444375152093 1107 24-25 0.3919556499601029 1107 26-27 0.5044851388993479 1107 28-29 0.49363148699913495 1107 30-31 0.35959270232006446 1107 32-33 0.2667603148899289 1107 34-35 0.42946029696319954 1107 36-37 0.5875527483142022 1107 38-39 0.34848214635452024 1107 40-41 0.4090999325136977 1107 42-43 0.5664416915905477 1107 44-45 0.2734987909616393 1107 46-47 0.4404501649261334 1107 48-49 0.9182426779596824 1107 50-51 1.3346663778330097 1107 52-53 1.569819440932136 1107 54-55 1.397124286095334 1107 56-57 1.590027753721678 1107 58-59 0.9869884668187936 1107 60-61 1.0670498983183165 1107 62-63 1.2406493067288658 1107 64-65 1.2439470169370175 1107 66-67 1.076812628621795 1107 68-69 1.09501496816722 1107 70-71 1.046762112448281 1107 72-73 1.2757405605491492 1107 74-75 1.3308441663327422 1107 76-77 0.8209687854748928 1107 78-79 1.147113157517552 1107 80-81 0.858047503104391 1107 82-83 0.9623881401026715 1107 84-85 0.9693257184616577 1107 86-87 1.237381672073699 1107 88-89 1.4864719935994053 1107 90-91 1.6249917635401196 1107 92-93 1.6281110820068463 1107 94-95 1.651435355180915 1107 96-97 1.4064489128426558 1107 98-99 1.574583573216401 1107 100-101 1.0300525885049154 1108 1 -0.1938853635010922 1108 2 -0.22181709521336757 1108 3 -0.20399868442987312 1108 4 -0.10443222344488845 1108 5 0.19592110956418196 1108 6 -0.04338421822638594 1108 7 0.13024675915021788 1108 8 0.004890580539878897 1108 9 0.2869396671413895 1108 10-11 0.1108305758286221 1108 12-13 0.10166909403642421 1108 14-15 0.18337131888142721 1108 16-17 0.04324012670947752 1108 18-19 0.09148603341752448 1108 20-21 0.006446315036015449 1108 22-23 -0.0847741656270884 1108 24-25 -0.1340318760274215 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132.0 9 251.0 10 458.0 11 756.0 12 1103.0 13 1508.0 14 1902.0 15 2795.0 16 3560.0 17 4695.0 18 4018.0 19 2186.0 20 1857.0 21 1830.0 22 1812.0 23 2013.0 24 2199.0 25 2510.0 26 2927.0 27 3514.0 28 4278.0 29 5196.0 30 6274.0 31 7554.0 32 9705.0 33 12603.0 34 16536.0 35 23230.0 36 32445.0 37 45781.0 38 31686.0 39 2143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88225396334971 21.21524631847921 16.32728897276489 19.57521074540619 2 15.78857288366249 23.71875952294482 38.92165825516691 21.57100933822578 3 19.644668567444928 26.686175386452156 32.73749639951409 20.931659646588827 4 12.491077205652147 19.908997933668676 38.028010269040514 29.571914591638667 5 11.751151111871794 39.24007831317757 34.83851037983561 14.170260195115029 6 27.32411063893736 38.8601317448258 18.117000759745196 15.698756856491647 7 24.36809918390349 36.82786833921229 21.502368975809315 17.30166350107491 8 24.777191041723196 33.76886310033186 20.735947903404227 20.717997954540714 9 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33.751750652776934 18.854243415353984 34-35 19.973658998833233 27.91874782144057 33.42593732845475 18.681655851271444 36-37 19.980504584762265 27.664286444841963 33.6464984439152 18.708710526480576 38-39 19.56802157387424 28.30631728790352 33.47269243453322 18.652968703689016 40-41 19.521089071504758 28.52258566815631 32.98106606941954 18.975259190919395 42-43 19.42141515341265 28.763097474431227 32.27029847630975 19.54518889584638 44-45 20.318050702767238 28.49327706186105 31.69048185578974 19.498190379581974 46-47 20.69491638628451 28.751972415405298 31.214882656937476 19.338228541372718 48-49 20.528479889795662 28.790674375404397 31.0183465175012 19.66249921729874 50-51 20.079313727536473 29.30203919767903 30.918578197073742 19.700068877710756 52-53 20.44374406445351 29.967334926581856 29.84585842351885 19.743062585445777 54-55 20.12673789759528 28.868981150033708 30.166625269516324 20.83765568285469 56-57 20.699551457190147 29.67520825201886 29.462729619897015 20.162510670893976 58-59 21.18783982634287 28.997401404702515 29.5818244434936 20.232934325461017 60-61 20.167020104194496 28.990156625701307 30.940255142933477 19.90256812717072 62-63 20.195362234142472 28.881259001064475 30.757237377637704 20.16614138715535 64-65 19.980839433197872 29.588465255296807 30.36824605674283 20.062449254762484 66-67 20.218947632067792 29.11335601427647 29.65707248857256 21.010623865083176 68-69 19.898770637223183 29.05073991358978 30.038821982425745 21.011667466761285 70-71 20.685855022854877 29.11335601427647 29.582350608419777 20.618438354448873 72-73 20.375279163448894 28.584876124480807 30.224791801465216 20.815052910605083 74-75 19.638701328518803 30.14287056073303 29.49896160549358 20.719466505254587 76-77 19.987142773654373 29.271062056989237 29.406730841480417 21.33506432787597 78-79 19.814947777954032 29.47331290648455 29.32052898302679 21.391210332534627 80-81 19.923728335945995 29.137069823848776 29.2637717945132 21.67543004569203 82-83 19.53408571470316 29.19342685571518 29.79037822922515 21.4821092003565 84-85 19.631928731851083 29.231337613652492 29.202950581516703 21.933783072979722 86-87 19.274183785498703 29.221961085530847 28.781553823611674 22.722301305358776 88-89 19.462040838476582 28.98914021293782 28.801499456280123 22.747319492305476 90-91 19.70591304710818 28.402871991818163 29.084970048631835 22.80624491244182 92-93 19.600550187953054 29.03201567910434 29.072507508729746 22.294926624212863 94-95 19.457327127382023 29.104172319509093 28.7224228256559 22.716077727452983 96-97 19.485452962945182 28.91916318271095 28.538658863162464 23.0567249911814 98-99 19.98801912765056 28.898114578049956 27.60797917749433 23.50588711680516 100-101 19.367611207587494 28.502162369756878 28.054034197168047 24.076192225487578 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1722.0 1 1085.0 2 442.5 3 415.5 4 339.5 5 339.5 6 534.0 7 908.5 8 1194.0 9 1203.0 10 997.5 11 760.0 12 703.5 13 724.0 14 708.5 15 683.5 16 694.5 17 730.0 18 772.0 19 817.0 20 910.5 21 1002.5 22 1144.5 23 1335.5 24 1530.0 25 1777.5 26 2235.0 27 2986.0 28 3392.0 29 3713.0 30 4083.0 31 4488.0 32 4977.0 33 5296.5 34 6097.5 35 6895.5 36 7402.0 37 7558.5 38 7204.0 39 6894.5 40 6857.5 41 6867.5 42 6912.5 43 6951.5 44 7094.0 45 7241.5 46 7332.0 47 8568.5 48 11818.5 49 11429.0 50 8019.5 51 6769.0 52 6041.5 53 5532.5 54 5156.5 55 4762.5 56 4364.5 57 3901.5 58 3626.5 59 3316.0 60 2749.0 61 2198.5 62 1719.0 63 1314.0 64 933.0 65 653.5 66 466.0 67 337.0 68 245.5 69 161.5 70 94.0 71 66.0 72 65.0 73 52.5 74 37.0 75 29.5 76 15.0 77 7.5 78 4.5 79 1.5 80 3.0 81 2.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02003715221974077 2 8.348813424891987E-4 3 0.0016697626849783973 4 0.0 5 0.0016697626849783973 6 4.1744067124459933E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012523220137337981 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0025046440274675962 22-23 0.0 24-25 0.003965686376823693 26-27 0.001878483020600697 28-29 0.0025046440274675962 30-31 0.014192982822316378 32-33 6.261610068668991E-4 34-35 0.001878483020600697 36-37 0.004800567719312893 38-39 0.0025046440274675962 40-41 0.004800567719312893 42-43 0.0020872033562229966 44-45 8.348813424891987E-4 46-47 4.1744067124459933E-4 48-49 0.0 50-51 0.0 52-53 0.0010436016781114983 54-55 0.0022959236918452964 56-57 6.261610068668991E-4 58-59 0.0010436016781114983 60-61 0.0012523220137337981 62-63 0.0 64-65 2.0872033562229967E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0010436016781114983 76-77 4.1744067124459933E-4 78-79 4.1744067124459933E-4 80-81 0.006887771075535889 82-83 0.001878483020600697 84-85 0.003756966041201394 86-87 0.0016697626849783973 88-89 6.261610068668991E-4 90-91 0.0 92-93 2.0872033562229967E-4 94-95 0.0 96-97 0.001878483020600697 98-99 0.002713364363089896 100-101 0.0012523220137337981 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 239555.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.162918654826825 #Duplication Level Percentage of deduplicated Percentage of total 1 82.32624785070752 51.17639848300644 2 11.402167070333562 14.175839681637806 3 3.078649660347172 5.741335452086146 4 1.1592831445150014 2.8825769524159153 5 0.5807717197729118 1.80512325866337 6 0.33212449885168377 1.2387496923235408 7 0.18425934607049016 0.8017869118820027 8 0.11894895721869486 0.5915371481329764 9 0.09674757240490535 0.5412700326112291 >10 0.6153345617147227 7.262643068134336 >50 0.05441219785431411 2.3864859482728056 >100 0.04433586491833002 5.650313449882648 >500 0.0026870221162624253 0.9604405391124153 >1k 0.004030533174393638 4.785499381838383 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2493 1.0406795934127862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2222 0.9275531715054998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1964 0.8198534783243931 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1823 0.7609943436789046 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1590 0.663730667278913 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1368 0.571058838262612 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 594 0.24795975871929202 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 581 0.24253302999311224 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 579 0.24169814865062303 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 546 0.22792260649955126 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.20663313226607669 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 443 0.1849262173613575 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 437 0.18242157333388992 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 431 0.17991692930642234 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 425 0.17741228527895472 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 414 0.17282043789526413 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 397 0.16572394648410596 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 357 0.14902631963432197 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 335 0.1398426248669408 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 327 0.136503099496984 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 280 0.11688338794848781 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 262 0.10936945586608503 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 260 0.10853457452359584 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 248 0.10352528646866063 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 245 0.10227296445492684 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 244 0.10185552378368225 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 243 0.10143808311243764 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 240 0.10018576109870383 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 240 0.10018576109870383 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.174406712445994E-4 0.0 20-21 0.0 0.0 0.0 4.174406712445994E-4 0.0 22-23 0.0 0.0 0.0 4.174406712445994E-4 0.0 24-25 0.0 0.0 0.0 0.002087203356222997 0.0 26-27 0.0 0.0 0.0 0.005218008390557492 0.0 28-29 0.0 0.0 0.0 0.011062177787981884 0.0 30-31 0.0 0.0 0.0 0.02045459289098537 0.0 32-33 0.0 0.0 0.0 0.03214293168583415 0.0 34-35 0.0 0.0 0.0 0.04028302477510384 0.0 36-37 0.0 0.0 0.0 0.05238880424119722 0.0 38-39 0.0 0.0 0.0 0.0672079480703805 0.0 40-41 0.0 0.0 0.0 0.08912358331072197 0.0 42-43 0.0 0.0 0.0 0.1350420571476279 0.0 44-45 0.0 0.0 0.0 0.1949447934712279 0.0 46-47 0.0 0.0 0.0 0.24962952140427042 0.0 48-49 0.0 0.0 0.0 0.3596251382772223 0.0 50-51 0.0 0.0 0.0 0.46941203481455196 0.0 52-53 0.0 0.0 0.0 0.5827471770574607 0.0 54-55 0.0 0.0 0.0 0.7574460979733255 0.0 56-57 0.0 0.0 0.0 0.9467554423827513 0.0 58-59 0.0 0.0 0.0 1.1746780488823025 0.0 60-61 0.0 0.0 0.0 1.432656383711465 0.0 62-63 0.0 0.0 0.0 1.767652522385256 0.0 64-65 0.0 0.0 0.0 2.0680010853457453 0.0 66-67 0.0 0.0 0.0 2.4351401557053705 0.0 68-69 0.0 0.0 0.0 2.887645843334516 0.0 70-71 0.0 0.0 0.0 3.315313811024608 0.0 72-73 0.0 0.0 0.0 3.769071820667488 0.0 74-75 0.0 0.0 0.0 4.329068481142118 0.0 76-77 0.0 0.0 0.0 4.817891507169543 0.0 78-79 0.0 0.0 0.0 5.32403832105362 0.0 80-81 0.0 0.0 0.0 5.9771242512157965 0.0 82-83 0.0 0.0 0.0 6.72538665442174 0.0 84-85 0.0 0.0 0.0 7.53856108200622 0.0 86-87 0.0 0.0 0.0 8.343177975830184 0.0 88-89 0.0 0.0 0.0 9.164283776168311 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGTAT 40 5.38064E-4 47.498123 8 TTAGGAT 60 8.2730294E-5 39.581764 4 GTCTAGA 60 0.0039414987 31.672026 1 TATAAGG 75 3.071943E-4 31.665417 2 GACTATC 30 0.0039494378 31.665413 40-41 ATAGCCC 85 6.3937483E-4 27.940071 3 GGACGTG 85 6.3937483E-4 27.940071 6 GTGTTAG 70 0.008371634 27.147448 1 ACGGGGC 35 0.008388392 27.141783 32-33 TAGAGCC 90 8.9288614E-4 26.387846 4 TACATGG 1280 0.0 25.233377 2 GTACATG 1320 0.0 24.833748 1 ACATGGG 1310 0.0 23.93035 3 TAGCCCT 145 2.3250897E-5 22.930128 4 CATGGGG 520 0.0 22.835636 4 CTATTAT 130 2.9287543E-4 21.926786 1 GAGTACT 1930 0.0 21.780228 12-13 GTATAGC 110 0.0028679746 21.594563 1 CGTTAGG 110 5.6243152E-8 21.590055 92-93 TCTAAAC 245 7.4469426E-9 21.325686 3 >>END_MODULE