##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 175397 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.023894365354025 31.0 31.0 34.0 30.0 34.0 2 32.09277809768696 33.0 31.0 34.0 30.0 34.0 3 32.18525402372902 33.0 31.0 34.0 30.0 34.0 4 35.935922507226465 37.0 35.0 37.0 35.0 37.0 5 35.84998033033632 37.0 35.0 37.0 35.0 37.0 6 35.91622433679025 37.0 35.0 37.0 35.0 37.0 7 35.87335017132562 37.0 35.0 37.0 35.0 37.0 8 35.863583755708476 37.0 35.0 37.0 35.0 37.0 9 37.483674179147876 39.0 37.0 39.0 35.0 39.0 10-11 37.409191719356656 39.0 37.0 39.0 34.0 39.0 12-13 37.331422430258215 39.0 37.0 39.0 34.0 39.0 14-15 38.419773428279846 40.0 38.0 41.0 34.0 41.0 16-17 38.372249810430056 40.0 38.0 41.0 34.0 41.0 18-19 38.441124990735304 40.0 38.0 41.0 34.0 41.0 20-21 38.53403992086524 40.0 38.0 41.0 34.0 41.0 22-23 38.443362771313076 40.0 38.0 41.0 34.0 41.0 24-25 38.321322485561325 40.0 38.0 41.0 34.0 41.0 26-27 38.070790834506866 40.0 37.0 41.0 33.5 41.0 28-29 37.89063096860266 40.0 37.0 41.0 33.0 41.0 30-31 37.685405109551475 39.5 36.5 41.0 33.0 41.0 32-33 37.44764163583186 39.0 35.5 41.0 33.0 41.0 34-35 37.2181280181531 39.0 35.0 41.0 33.0 41.0 36-37 36.95222267199553 39.0 35.0 41.0 32.0 41.0 38-39 36.70144871348997 39.0 35.0 41.0 31.0 41.0 40-41 36.39179404436791 39.0 35.0 41.0 31.0 41.0 42-43 36.03001191582524 39.0 35.0 41.0 30.0 41.0 44-45 35.5669851821867 39.0 35.0 40.5 28.5 41.0 46-47 34.92409505293705 38.5 35.0 40.0 25.0 41.0 48-49 34.320279138183665 38.0 34.5 40.0 21.5 41.0 50-51 33.965156188532305 38.0 34.0 40.0 18.5 41.0 52-53 33.68376597091171 38.0 34.0 40.0 14.0 41.0 54-55 33.36149135960136 38.0 33.0 40.0 8.5 41.0 56-57 33.047580631367694 38.0 33.0 40.0 6.0 41.0 58-59 32.70290825954834 37.0 33.0 40.0 2.0 41.0 60-61 32.31009367320992 37.0 32.0 40.0 2.0 41.0 62-63 31.95963727999909 36.0 32.0 39.0 2.0 41.0 64-65 31.508409493891 36.0 31.0 39.0 2.0 40.0 66-67 31.038546839455634 35.0 31.0 39.0 2.0 40.0 68-69 30.54993243898128 35.0 30.0 38.0 2.0 40.0 70-71 30.105740120982688 35.0 30.0 37.5 2.0 40.0 72-73 29.74633545613665 35.0 29.0 37.0 2.0 39.0 74-75 29.288305957342487 34.0 29.0 36.5 2.0 39.0 76-77 27.847480287576182 32.5 27.0 35.0 2.0 37.5 78-79 28.48606874689989 34.0 28.5 35.5 2.0 37.5 80-81 28.08052874336505 34.0 27.0 35.0 2.0 37.0 82-83 27.942188292844236 34.0 27.0 35.0 2.0 37.0 84-85 27.627493628739373 34.0 27.0 35.0 2.0 36.0 86-87 27.252852101233202 34.0 26.0 35.0 2.0 36.0 88-89 26.858666339789167 33.0 25.5 35.0 2.0 35.5 90-91 26.367212666123137 33.0 25.0 35.0 2.0 35.0 92-93 25.92119306487568 33.0 23.5 35.0 2.0 35.0 94-95 25.38972160299207 32.5 20.0 35.0 2.0 35.0 96-97 24.75882996858555 32.0 14.5 34.0 2.0 35.0 98-99 24.007879268174484 32.0 2.0 34.0 2.0 35.0 100-101 21.256221600141394 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12562106405591678 1101 2 0.09872080147254181 1101 3 0.03192366869812702 1101 4 0.03451385489479719 1101 5 0.03145594699569898 1101 6 0.22212632933167953 1101 7 0.2026233884762405 1101 8 0.2389160784258877 1101 9 0.21744379246835877 1101 10-11 0.23292135014521165 1101 12-13 0.11040087073365612 1101 14-15 0.21653398682448 1101 16-17 0.19837569478518446 1101 18-19 0.3260884299420397 1101 20-21 -0.08373848046726806 1101 22-23 0.25648224562020516 1101 24-25 0.3014150506367699 1101 26-27 0.15182252932881113 1101 28-29 0.1840938855827332 1101 30-31 0.3839933842972343 1101 32-33 0.3150654476916017 1101 34-35 0.3634824945601949 1101 36-37 0.3216339395736014 1101 38-39 0.33117056030301484 1101 40-41 0.4327797593070173 1101 42-43 0.590078943220945 1101 44-45 0.5818804464468883 1101 46-47 0.38816011061686595 1101 48-49 0.41342102265034697 1101 50-51 0.3155394414272408 1101 52-53 0.47764150620458423 1101 54-55 0.6075609715014494 1101 56-57 0.3751766534416703 1101 58-59 0.5837947564753776 1101 60-61 0.7066352026175515 1101 62-63 0.5527290819389741 1101 64-65 0.6829928532620144 1101 66-67 0.5942947642348742 1101 68-69 0.41957358525988653 1101 70-71 0.7936236723404555 1101 72-73 0.9473992126239246 1101 74-75 0.9698486237557127 1101 76-77 0.700319986176364 1101 78-79 1.3103875941740775 1101 80-81 1.374556812200968 1101 82-83 1.2056177007352566 1101 84-85 1.1032324509164724 1101 86-87 1.4594645567452105 1101 88-89 1.24216340917895 1101 90-91 0.8601452002042649 1101 92-93 1.2888996219366504 1101 94-95 0.8180384278871777 1101 96-97 0.7800855136948819 1101 98-99 1.101513242225593 1101 100-101 0.9856614049587122 1102 1 0.30854469615757907 1102 2 0.2527211715915989 1102 3 0.47600484814418564 1102 4 0.1987541855344901 1102 5 0.3210201318084813 1102 6 0.057739493232730865 1102 7 0.3010596354690236 1102 8 0.1608286686424094 1102 9 0.38123689591663634 1102 10-11 0.23179557135303241 1102 12-13 0.09431611347007163 1102 14-15 0.22869008453591988 1102 16-17 0.44083451904032245 1102 18-19 0.47051582492075994 1102 20-21 0.2335345131790163 1102 22-23 0.3119731160168513 1102 24-25 0.3497618521912713 1102 26-27 0.39595845555174947 1102 28-29 0.1247900178386061 1102 30-31 0.45248468649943874 1102 32-33 0.4227546710584562 1102 34-35 0.45677562885172307 1102 36-37 0.7939482631810222 1102 38-39 0.64102960662958 1102 40-41 0.998009387337369 1102 42-43 0.6426512706529408 1102 44-45 0.877513041598327 1102 46-47 1.0462433565609786 1102 48-49 1.3050031273940448 1102 50-51 1.2614288066730595 1102 52-53 1.4754516351293958 1102 54-55 1.4551451735864518 1102 56-57 1.6617143438987654 1102 58-59 1.710533544952341 1102 60-61 1.7988449675919505 1102 62-63 1.806565211727385 1102 64-65 1.7049455397094881 1102 66-67 1.8319805948437278 1102 68-69 1.8648653624482172 1102 70-71 1.7913644039424526 1102 72-73 1.871042880503758 1102 74-75 1.9069206735984103 1102 76-77 1.7614937262294141 1102 78-79 1.75699792049571 1102 80-81 1.8581867548325128 1102 82-83 2.1254252388266757 1102 84-85 1.9748049942512438 1102 86-87 1.9283475682805857 1102 88-89 2.377029395237802 1102 90-91 2.3237231719518405 1102 92-93 2.299058618914014 1102 94-95 1.8887350227918702 1102 96-97 1.9689618939305227 1102 98-99 2.198765879323858 1102 100-101 2.9064135177216492 1103 1 0.2164181687630169 1103 2 0.17124698948675032 1103 3 0.10430599122656758 1103 4 0.24569939641347815 1103 5 0.19119159416559484 1103 6 0.17158458586182235 1103 7 0.11507224879184719 1103 8 0.23494179438147 1103 9 -0.019610561710479146 1103 10-11 0.31096388037943967 1103 12-13 0.19003940870066316 1103 14-15 0.21292835808735333 1103 16-17 -0.07185763704955406 1103 18-19 0.22994132748284102 1103 20-21 0.03635772957633776 1103 22-23 0.0816272350550804 1103 24-25 0.4296396606264281 1103 26-27 0.38875999280046614 1103 28-29 0.4129748818512198 1103 30-31 -0.025456779799334583 1103 32-33 0.26187568012428386 1103 34-35 0.27032509672717 1103 36-37 0.5399228394522098 1103 38-39 0.23902921246324382 1103 40-41 0.5100265335730754 1103 42-43 0.057255133483053555 1103 44-45 0.27625170931610654 1103 46-47 0.43553681339976436 1103 48-49 0.08915271351938259 1103 50-51 -0.04792771677983154 1103 52-53 0.03780874983179672 1103 54-55 0.20218399897076011 1103 56-57 0.16471493514826818 1103 58-59 0.22386129426254797 1103 60-61 0.19898785288954812 1103 62-63 0.32481018610657486 1103 64-65 0.3111635136945097 1103 66-67 0.7649821715090752 1103 68-69 0.7017297692278781 1103 70-71 1.2890289683886458 1103 72-73 1.119421871437936 1103 74-75 1.1843250882613994 1103 76-77 0.8871293194497518 1103 78-79 0.4399699346856991 1103 80-81 0.8446372869570702 1103 82-83 0.9886101028392851 1103 84-85 1.0471788610230206 1103 86-87 0.5074616794354938 1103 88-89 0.1488711374530176 1103 90-91 0.07741355429319086 1103 92-93 0.43364178801137143 1103 94-95 0.38333987103782974 1103 96-97 0.956338716949837 1103 98-99 1.4462284335217284 1103 100-101 0.7163317282064767 1104 1 0.311265626390135 1104 2 0.2605351250799721 1104 3 0.23386531326046622 1104 4 0.16861465065076686 1104 5 -0.11891010074965891 1104 6 0.30385577230249794 1104 7 0.105106147096933 1104 8 0.12043331980519412 1104 9 -0.08076310408336695 1104 10-11 0.312353710887912 1104 12-13 0.4129207646328723 1104 14-15 0.25872496825684976 1104 16-17 0.15153219345892666 1104 18-19 0.40817861561843216 1104 20-21 0.3274763736441315 1104 22-23 0.2057289299703342 1104 24-25 0.17743627079591562 1104 26-27 0.279132874156403 1104 28-29 -0.0014657961148785148 1104 30-31 0.10535677952270106 1104 32-33 0.36363839198868675 1104 34-35 0.4704267916424243 1104 36-37 0.5234482631810238 1104 38-39 0.5102156531412092 1104 40-41 0.3614163640815562 1104 42-43 0.651628014838991 1104 44-45 0.43486187880763083 1104 46-47 -0.15476827134600057 1104 48-49 0.08589847623125024 1104 50-51 0.262207876440506 1104 52-53 0.1988934955945112 1104 54-55 0.5403195921911035 1104 56-57 0.6319352741313224 1104 58-59 0.23633587053372906 1104 60-61 0.7619031071268374 1104 62-63 0.7564373047506443 1104 64-65 0.9190618187792587 1104 66-67 0.7762364087972102 1104 68-69 1.0738653624482204 1104 70-71 0.6226899853378001 1104 72-73 0.18765915957352775 1104 74-75 0.00703695266817661 1104 76-77 0.4609937262294146 1104 78-79 1.0982072228212907 1104 80-81 0.8445355920418187 1104 82-83 1.1081694248731857 1104 84-85 0.888839877972174 1104 86-87 1.23698710316431 1104 88-89 0.8002270696564047 1104 90-91 0.62836270683556 1104 92-93 1.1333028049605254 1104 94-95 1.4537466506988466 1104 96-97 1.1441014288142455 1104 98-99 1.1289402979285121 1104 100-101 1.425518168884441 1105 1 0.3134967821690289 1105 2 0.24974115523072626 1105 3 0.4002773735619698 1105 4 0.05016238934423711 1105 5 0.12458738668753 1105 6 0.0825291391366676 1105 7 0.12152825764969322 1105 8 0.008453420307709791 1105 9 -0.12253194830447 1105 10-11 0.35254466566178166 1105 12-13 0.1749810661404041 1105 14-15 0.4621320034327212 1105 16-17 0.6385673693383254 1105 18-19 0.3299072588345098 1105 20-21 0.41774773042806146 1105 22-23 0.16029174404068414 1105 24-25 0.31251164768034556 1105 26-27 0.5436956882267481 1105 28-29 0.39990103805596533 1105 30-31 0.010894467964909893 1105 32-33 0.5443218090741127 1105 34-35 0.5168288016926752 1105 36-37 0.4927547958443341 1105 38-39 0.5629794722366839 1105 40-41 0.8762304344333174 1105 42-43 1.3900802761455253 1105 44-45 1.5015050948880315 1105 46-47 1.472407608050986 1105 48-49 1.259828124472456 1105 50-51 1.146238027194272 1105 52-53 1.044401033282945 1105 54-55 1.452128637417232 1105 56-57 1.5463774851865963 1105 58-59 1.65444642329755 1105 60-61 1.5587272277298503 1105 62-63 1.484949867564719 1105 64-65 1.8415442308395562 1105 66-67 1.382246459048467 1105 68-69 0.9647397343075141 1105 70-71 0.9335141059408159 1105 72-73 0.5827043857041794 1105 74-75 0.8852279074420473 1105 76-77 0.8924610629128331 1105 78-79 1.0414434037258147 1105 80-81 1.5041637327453365 1105 82-83 1.365988520350573 1105 84-85 1.1378951543540836 1105 86-87 1.3316504197472199 1105 88-89 0.8910310897569076 1105 90-91 1.3124531590968687 1105 92-93 1.248478684357508 1105 94-95 1.423626047683772 1105 96-97 1.4900411273067071 1105 98-99 1.6109503481797667 1105 100-101 1.5181714352160967 1106 1 0.2329097981692776 1106 2 0.08215475698181152 1106 3 0.24915365681874846 1106 4 0.26331403715383317 1106 5 -0.008038935105489031 1106 6 0.03742632444973992 1106 7 0.15121657654478327 1106 8 0.37621246090949256 1106 9 0.3122184910086574 1106 10-11 0.4566849992314772 1106 12-13 0.2966630959212111 1106 14-15 0.5856329437169663 1106 16-17 0.418648757876106 1106 18-19 0.4725221739006358 1106 20-21 0.41316962517787914 1106 22-23 0.32564304039978254 1106 24-25 -0.3896802936212609 1106 26-27 0.172237168634922 1106 28-29 0.22653420388511591 1106 30-31 0.43587211694600825 1106 32-33 0.6309512754242732 1106 34-35 0.4302918223172725 1106 36-37 0.24371820183132797 1106 38-39 1.2402770028344605 1106 40-41 1.0588151370876844 1106 42-43 1.1615912050230364 1106 44-45 0.7452545168444402 1106 46-47 1.4679065752797698 1106 48-49 1.7732849792987366 1106 50-51 0.8267109439251641 1106 52-53 1.1478014710546276 1106 54-55 1.4890925983260743 1106 56-57 0.4959720839472723 1106 58-59 1.1495137846441565 1106 60-61 1.357706788108434 1106 62-63 1.0560569366524781 1106 64-65 0.8150004690860051 1106 66-67 1.3514020529689894 1106 68-69 1.3303561599942277 1106 70-71 1.3367881448470023 1106 72-73 1.2613892209232205 1106 74-75 0.9556259097233948 1106 76-77 0.9014477139594739 1106 78-79 1.4316305357047305 1106 80-81 1.6331368190356805 1106 82-83 1.231003780701407 1106 84-85 0.7642631908556119 1106 86-87 0.7867171645140019 1106 88-89 1.0461779899018033 1106 90-91 1.0409884737067259 1106 92-93 1.5537936025065342 1106 94-95 1.9900779390424042 1106 96-97 1.3407517355627085 1106 98-99 1.1844004206278989 1106 100-101 1.1881316658169538 1107 1 0.03562005676988278 1107 2 -0.02943955846433255 1107 3 -0.3669448133218154 1107 4 -0.25811952656442116 1107 5 -0.1419987083445946 1107 6 -0.1432834682038333 1107 7 -0.3071976503714211 1107 8 -0.1879717434859387 1107 9 -0.020661838260579657 1107 10-11 -0.07362097265639278 1107 12-13 0.1933005114683084 1107 14-15 0.1997123100289997 1107 16-17 -0.20517666730057016 1107 18-19 -0.38227708058410315 1107 20-21 -0.626397044077379 1107 22-23 -0.35860018395371895 1107 24-25 -0.1076270203433225 1107 26-27 -0.00684180938790746 1107 28-29 -0.6089088340895685 1107 30-31 -0.49980777743932947 1107 32-33 -0.31767806370751117 1107 34-35 -0.37369979063605996 1107 36-37 -0.6008302178316356 1107 38-39 0.20325362782475054 1107 40-41 0.12037838939800594 1107 42-43 0.36291915407949205 1107 44-45 0.7475454231114327 1107 46-47 0.6144469185274204 1107 48-49 0.9694680964844125 1107 50-51 0.3864104080860713 1107 52-53 -0.09571409934219588 1107 54-55 0.40361073143160553 1107 56-57 0.3864162867895544 1107 58-59 0.02663966800209039 1107 60-61 0.2489663982660808 1107 62-63 0.6881335072822914 1107 64-65 -0.21759640906884314 1107 66-67 0.2966921049997424 1107 68-69 0.5689792864988483 1107 70-71 0.509829225844129 1107 72-73 0.6027730836241574 1107 74-75 -0.0567605156862534 1107 76-77 0.04076587812814836 1107 78-79 1.0055996278845853 1107 80-81 0.9133457186240967 1107 82-83 0.4502706906959695 1107 84-85 0.11354873873166937 1107 86-87 0.34329090063265966 1107 88-89 0.3030118797829857 1107 90-91 0.2892994156963198 1107 92-93 0.7765939442010286 1107 94-95 0.33951880259758127 1107 96-97 0.4843799098269024 1107 98-99 0.5886871333715469 1107 100-101 1.0520751309097562 1108 1 -0.06909800997350146 1108 2 0.35701997356482096 1108 3 0.372410767805917 1108 4 0.25425101428713504 1108 5 0.33872626288670915 1108 6 -0.1551745307278054 1108 7 -0.13410597411519376 1108 8 -0.030415165043287118 1108 9 0.21457022924997204 1108 10-11 0.3317779533121552 1108 12-13 0.2023147040268043 1108 14-15 -0.08821442568254412 1108 16-17 0.12925946618619832 1108 18-19 0.5932695247093349 1108 20-21 0.49150667667444026 1108 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-1.2032603288717496 2316 66-67 -1.3452669469074863 2316 68-69 -1.661141348961177 2316 70-71 -1.1789610213736061 2316 72-73 -1.7531864780103668 2316 74-75 -1.608667745318396 2316 76-77 -1.6902680187370294 2316 78-79 -0.9057927771787071 2316 80-81 -1.6516657502400598 2316 82-83 -2.2736695013013097 2316 84-85 -2.4825560951821863 2316 86-87 -2.5835632324061635 2316 88-89 -2.6724574941019874 2316 90-91 -2.1076507159832296 2316 92-93 -1.8672341077911554 2316 94-95 -2.138159389569612 2316 96-97 -2.7757509201790462 2316 98-99 -2.9655563463667924 2316 100-101 -2.300401294202807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 7.0 6 24.0 7 63.0 8 82.0 9 177.0 10 352.0 11 506.0 12 845.0 13 1189.0 14 1384.0 15 2122.0 16 2765.0 17 3707.0 18 3168.0 19 1780.0 20 1450.0 21 1462.0 22 1532.0 23 1571.0 24 1686.0 25 1837.0 26 2099.0 27 2529.0 28 2946.0 29 3743.0 30 4415.0 31 5366.0 32 6739.0 33 8677.0 34 11723.0 35 16707.0 36 23840.0 37 33523.0 38 23854.0 39 1524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34223013048636 21.103659092982937 16.373528606624692 19.180582169906014 2 15.729664389732129 24.00816503403998 39.0661527408742 21.196017835353683 3 19.582261807689896 26.90665039658328 32.85340388771362 20.657683908013205 4 12.41982474044596 20.366368866058142 38.0194644150128 29.194341978483095 5 11.85848276648516 39.147589588619326 34.75211677167375 14.24181087322177 6 26.82600044470544 39.150612610249894 18.153104100982343 15.870282844062327 7 24.010672930551834 37.49265950956972 21.46444922091028 17.032218338968168 8 24.609314868555334 33.73717908516109 20.963870533703542 20.689635512580033 9 25.226201132288466 18.16849775081672 22.340746991111594 34.26455412578323 10-11 23.689116689567097 27.896144175784077 27.726814027605943 20.68792510704288 12-13 26.10449437561646 27.209986487796257 27.87134329549536 18.814175841091924 14-15 22.28544387874365 28.26160082555574 29.145310353084717 20.307644942615894 16-17 18.514569804500645 31.160738211029837 29.69206998979458 20.63262199467494 18-19 18.3822816710284 29.497573964159667 33.70536350626322 18.414780858548713 20-21 20.304682845340956 27.093910619143593 33.58638049436844 19.015026041147006 22-23 19.52142853070463 27.885881742561164 33.6701882016226 18.922501525111603 24-25 20.033353763701413 28.091851133885427 34.023404649571674 17.851390452841486 26-27 19.56496329555983 27.530183165847056 34.90243033283444 18.002423205758678 28-29 18.531537310241607 28.910840282232197 34.222507305252655 18.335115102273537 30-31 19.397307616633352 28.151403079046343 33.83814333334284 18.613145970977467 32-33 18.90750074117996 28.336165020867064 34.074893384113665 18.681440853839312 34-35 19.533443752975487 28.149881835778817 33.74593409600807 18.570740315237625 36-37 19.77402901757077 28.11636678802462 33.90391529081718 18.205688903587426 38-39 19.255878395329212 28.72744999657901 33.65595137637695 18.36072023171483 40-41 19.202260196256194 28.96974051076786 33.2401087904757 18.587890502500244 42-43 19.05666025229848 29.355569809707077 32.32841565105837 19.25935428693607 44-45 20.05216870732785 28.950496743019226 31.65186652793021 19.34546802172271 46-47 20.375897624484374 29.07519377856078 31.382291818111845 19.166616778843 48-49 20.142763069748085 29.3562263570046 31.03756937891793 19.463441194329388 50-51 19.81316031039221 29.695081275550056 31.05547034910571 19.43628806495202 52-53 20.2056512765545 30.45742727801407 29.890133071826856 19.44678837360457 54-55 19.578950369166737 29.461216112203882 30.19584366715129 20.763989851478094 56-57 20.380110207444275 30.11462600450978 29.549335363020358 19.955928425025586 58-59 20.803781158065178 29.534881069123582 29.67057401537093 19.990763757440305 60-61 19.9362018734642 29.245654144597683 31.05754374362161 19.760600238316506 62-63 19.828731392213093 29.110247039573085 30.822933117442147 20.238088450771677 64-65 19.626368049992447 29.95042377834098 30.525724613924105 19.897483557742472 66-67 19.739902848411596 29.27005176856941 29.973032452279412 21.017012930739583 68-69 19.515497743683596 29.349502413104023 30.13543599786769 20.99956384534469 70-71 20.40799454943 29.27771395340219 29.825336598353996 20.488954898813816 72-73 19.937733783413922 28.69691405893623 30.394979928838612 20.97037222881124 74-75 19.316941059083458 30.105623262775282 29.737010904425915 20.840424773715345 76-77 19.47499558125082 29.322534480497637 29.63526789023257 21.567202048018974 78-79 19.473636192572403 29.558712002417703 29.287005411323292 21.6806463936866 80-81 19.62975421472431 29.284423336773145 29.087726700000573 21.99809574850197 82-83 19.06613565806391 29.212980252523668 29.81250302897266 21.908381060439762 84-85 19.10015308173196 29.30189598994279 29.29106339066059 22.306887537664664 86-87 18.882021927398988 29.320056785463834 28.680935249747712 23.116986037389463 88-89 18.93036024299369 28.998748542579484 28.702275714029152 23.36861550039767 90-91 19.378379842243145 28.418469011639342 29.05046565923493 23.152685486882575 92-93 19.157451830566743 29.088094688320464 28.789057934451375 22.965395546661423 94-95 19.089659969440582 29.449930443111587 28.1836529909462 23.27675659650163 96-97 19.167089516924463 29.205459614474595 28.011881617131422 23.615569251469523 98-99 19.82388524319116 29.330604286276273 26.96083741455099 23.884673055981573 100-101 19.404376382585692 28.595007867910326 27.30449953248649 24.69611621701749 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1244.0 1 802.0 2 334.0 3 299.0 4 274.5 5 280.5 6 377.5 7 646.5 8 871.0 9 859.5 10 724.5 11 625.5 12 580.5 13 548.0 14 574.0 15 555.0 16 540.5 17 558.0 18 583.5 19 619.5 20 675.0 21 778.5 22 905.0 23 1058.5 24 1187.5 25 1402.5 26 1725.5 27 2181.0 28 2522.0 29 2800.5 30 3091.0 31 3429.0 32 3724.5 33 3894.0 34 4479.0 35 5045.5 36 5471.5 37 5619.5 38 5320.5 39 5058.5 40 4982.0 41 5027.5 42 5041.5 43 5127.5 44 5228.0 45 5322.5 46 5440.5 47 6381.0 48 8850.5 49 8400.0 50 5772.5 51 4932.5 52 4387.0 53 3950.5 54 3629.5 55 3304.0 56 3032.5 57 2657.0 58 2387.0 59 2236.0 60 1925.5 61 1515.5 62 1202.5 63 889.0 64 608.5 65 463.0 66 323.0 67 214.0 68 152.0 69 112.0 70 78.0 71 48.5 72 36.0 73 28.0 74 19.0 75 17.5 76 14.0 77 7.5 78 2.5 79 2.5 80 3.0 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03021716448970051 2 0.008552027685764295 3 0.01368324429722287 4 0.0 5 0.006841622148611435 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004561081432407624 20-21 8.552027685764294E-4 22-23 0.0 24-25 0.0025656083057292887 26-27 0.0054162842009840536 28-29 0.0054162842009840536 30-31 0.028791826542073125 32-33 5.70135179050953E-4 34-35 0.003135743484780241 36-37 0.011687771170544536 38-39 0.005131216611458577 40-41 0.007981892506713342 42-43 0.0054162842009840536 44-45 0.0025656083057292887 46-47 0.0019954731266783356 48-49 8.552027685764294E-4 50-51 0.0034208110743057176 52-53 0.0017104055371528588 54-55 0.001140270358101906 56-57 2.850675895254765E-4 58-59 5.70135179050953E-4 60-61 0.0 62-63 0.0 64-65 0.005986419380035007 66-67 5.70135179050953E-4 68-69 2.850675895254765E-4 70-71 0.0014253379476273824 72-73 0.011972838760070013 74-75 0.0054162842009840536 76-77 0.004561081432407624 78-79 0.01368324429722287 80-81 0.0 82-83 0.004276013842882147 84-85 2.850675895254765E-4 86-87 0.0 88-89 8.552027685764294E-4 90-91 2.850675895254765E-4 92-93 2.850675895254765E-4 94-95 5.70135179050953E-4 96-97 0.0 98-99 0.0 100-101 5.70135179050953E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 175397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.607632281660585 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61027053648445 50.894231699002326 2 11.368013170377788 14.007127503474184 3 2.947846483918177 5.448295266120513 4 1.085053820468674 2.673903871089801 5 0.4829991844402075 1.4878218073667129 6 0.27941062546051876 1.0328296241376256 7 0.18361178308791998 0.791832105054242 8 0.14070241400840922 0.6934674066697635 9 0.11276149905282648 0.6252272072257816 >10 0.669583759691093 7.884502284710377 >50 0.06186914022180389 2.612798027671195 >100 0.05089235727922579 6.009435463643749 >500 9.978893584161918E-4 0.32583128345076084 >1k 0.0059873361504971506 5.5126964503829665 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1861 1.0610215682138235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.9618180470589577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1618 0.9224787197044418 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1452 0.8278362799819838 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.7143793793508441 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.6248681562398445 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 530 0.30217164489700504 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.2628323175424893 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 443 0.25256988431957217 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 417 0.2377463696642474 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.20410839410024118 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 349 0.19897717748878258 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 320 0.18244325729630495 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 300 0.1710405537152859 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 297 0.16933014817813302 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 280 0.15963785013426685 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 277 0.15792744459711397 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 267 0.15222609280660446 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 249 0.14196365958368728 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 237 0.13512203743507584 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 223 0.1271401449283625 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 215 0.12257906349595489 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 203 0.11573744134734346 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 201 0.11459717098924155 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 192 0.10946595437778296 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 182 0.10376460258727345 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 179 0.10205419705012059 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 176 0.10034379151296771 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.70135179050953E-4 0.0 18-19 0.0 0.0 0.0 5.70135179050953E-4 0.0 20-21 0.0 0.0 0.0 5.70135179050953E-4 0.0 22-23 0.0 0.0 0.0 5.70135179050953E-4 0.0 24-25 0.0 0.0 0.0 0.001710405537152859 0.0 26-27 0.0 0.0 0.0 0.0037058786638311946 0.0 28-29 0.0 0.0 0.0 0.013113109118171918 0.0 30-31 0.0 0.0 0.0 0.02451581269919098 0.0 32-33 0.0 0.0 0.0 0.04219000324977052 0.0 34-35 0.0 0.0 0.0 0.05672845031556982 0.0 36-37 0.0 0.0 0.0 0.0667058159489615 0.0 38-39 0.0 0.0 0.0 0.08095919542523533 0.0 40-41 0.0 0.0 0.0 0.10918088678825749 0.0 42-43 0.0 0.0 0.0 0.1624885260295216 0.0 44-45 0.0 0.0 0.0 0.24829387047669002 0.0 46-47 0.0 0.0 0.0 0.32868293072287436 0.0 48-49 0.0 0.0 0.0 0.47834341522374957 0.0 50-51 0.0 0.0 0.0 0.6399767384846947 0.0 52-53 0.0 0.0 0.0 0.8030353996932673 0.0 54-55 0.0 0.0 0.0 1.0270985250602918 0.0 56-57 0.0 0.0 0.0 1.285654828759899 0.0 58-59 0.0 0.0 0.0 1.6103468132294165 0.0 60-61 0.0 0.0 0.0 1.9433057577951731 0.0 62-63 0.0 0.0 0.0 2.391146940939697 0.0 64-65 0.0 0.0 0.0 2.8073456216468924 0.0 66-67 0.0 0.0 0.0 3.2871143748182696 0.0 68-69 0.0 0.0 0.0 3.937923681704932 0.0 70-71 0.0 0.0 0.0 4.454466153925096 0.0 72-73 0.0 0.0 0.0 5.055673700234326 0.0 74-75 0.0 0.0 0.0 5.796279297821513 0.0 76-77 0.0 0.0 0.0 6.457351037931094 0.0 78-79 0.0 0.0 0.0 7.078798383096633 0.0 80-81 0.0 0.0 0.0 7.9015034464671565 0.0 82-83 0.0 0.0 0.0 8.891543184889137 0.0 84-85 0.0 0.0 0.0 9.935460697731433 0.0 86-87 0.0 0.0 0.0 10.916948408467647 0.0 88-89 0.0 0.0 0.0 11.902712133046744 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAGT 70 0.008352799 27.155981 1 CCTTGAC 35 0.008387323 27.140495 42-43 TACAGTG 90 8.906897E-4 26.39412 5 CATGGGG 365 0.0 23.429298 4 TAGGCAT 105 0.0021863384 22.62353 5 GGGGTGA 175 4.020005E-6 21.712397 7 GAGTACT 1560 0.0 21.160017 12-13 TACATGG 970 0.0 21.066881 2 ACATGGG 985 0.0 20.746065 3 AGAACTA 115 0.0037066287 20.656267 5 AGTACTT 1595 0.0 20.39791 12-13 GTACATG 1005 0.0 20.333208 1 GTACTTT 1660 0.0 19.885317 14-15 GACAGAG 120 0.004750101 19.789944 7 ATGGGGT 200 1.2724406E-5 19.003765 5 TACTTTT 1765 0.0 18.16414 14-15 CCAGAAG 185 1.5064634E-4 17.981663 3 ATGGGGA 185 1.5097603E-4 17.976534 5 TTATAGG 160 0.0011817161 17.821114 2 AGAGTAC 2700 0.0 17.679016 10-11 >>END_MODULE