FastQCFastQC Report
Thu 26 May 2016
SRR936797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186165
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19351.0394005317863186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17180.9228372680149329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.8583783203072543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14960.8035882147557275No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.6859506351892138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.6005425294765396No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5000.26857894878199445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.24977842236725487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4420.2374237907232831No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4290.23044073805495124No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3790.2035828431767518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.18800526414739613No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3470.18639379045470417No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3330.1788735798888083No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3260.17511347460586038No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3130.16813042193752853No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2770.14879273762522494No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2750.14771842183009695No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC2680.14395831654714905No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT2470.13267800069830527No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2410.12945505331292134No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2150.11548894797625761No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2130.11441463218112963No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2110.11334031638600167No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1870.10044852684446594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT500.001606208238.029461
GCCTGAT702.0471256E-433.9275177
ATAAGGT600.003943125731.6656823
TATTATA1205.34008E-627.7149142
GTGCACT700.00837510327.1420144
AAACTAT908.9209125E-426.388076
TCTAAAC1501.0524273E-625.3325463
AACTTAC1351.3352024E-524.6288647
AATCTAA1401.7585204E-523.7684141
AGTACTT15950.022.93032312-13
CGGTGTT1100.002868932221.5902374
GAGTACT15450.021.3666512-13
CATGGGT1604.961717E-520.7806034
ACTTACT1604.961717E-520.7806038
GTACATG9450.020.6244431
TACATGG9650.020.1861422
ATCTAAA1656.274883E-520.1563032
ATGTCCA1200.00474964519.791054
CTTACTT1707.910153E-519.5582169
CTAAACT2451.7369348E-719.3871524
Produced by FastQC (version 0.11.5)