##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 186165 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05117503290092 31.0 31.0 34.0 30.0 34.0 2 32.16884484194129 33.0 31.0 34.0 30.0 34.0 3 32.22894206752075 34.0 31.0 34.0 30.0 34.0 4 35.948062202884536 37.0 35.0 37.0 35.0 37.0 5 35.850739935003894 37.0 35.0 37.0 35.0 37.0 6 35.91891064378374 37.0 35.0 37.0 35.0 37.0 7 35.85819568662208 37.0 35.0 37.0 35.0 37.0 8 35.838105981253186 37.0 35.0 37.0 35.0 37.0 9 37.47045362984449 39.0 37.0 39.0 35.0 39.0 10-11 37.397432385249644 39.0 37.0 39.0 34.0 39.0 12-13 37.31787392904144 39.0 37.0 39.0 34.0 39.0 14-15 38.394453844707655 40.0 38.0 41.0 34.0 41.0 16-17 38.34120269653265 40.0 38.0 41.0 33.5 41.0 18-19 38.50258910106626 40.0 38.0 41.0 34.0 41.0 20-21 38.54261810759273 40.0 38.0 41.0 34.0 41.0 22-23 38.43697257808933 40.0 38.0 41.0 34.0 41.0 24-25 38.30025246421185 40.0 38.0 41.0 34.0 41.0 26-27 38.05456449923455 40.0 37.0 41.0 33.5 41.0 28-29 37.89789165525206 40.0 37.0 41.0 33.0 41.0 30-31 37.78230601885424 40.0 37.0 41.0 33.0 41.0 32-33 37.486023151505385 40.0 35.5 41.0 33.0 41.0 34-35 37.23807106598984 39.0 35.0 41.0 33.0 41.0 36-37 36.99068836784573 39.0 35.0 41.0 32.5 41.0 38-39 36.72723659119598 39.0 35.0 41.0 31.0 41.0 40-41 36.473861896704534 39.0 35.0 41.0 31.0 41.0 42-43 36.07700964198426 39.0 35.0 41.0 30.0 41.0 44-45 35.59639567050735 39.0 35.0 40.0 29.0 41.0 46-47 34.950044315526554 38.5 35.0 40.0 25.0 41.0 48-49 34.373765208282975 38.0 34.0 40.0 22.0 41.0 50-51 33.952160717642954 38.0 34.0 40.0 19.0 41.0 52-53 33.65968629978782 38.0 33.5 40.0 16.0 41.0 54-55 33.3496575618403 38.0 33.0 40.0 10.0 41.0 56-57 32.98485214728869 38.0 33.0 40.0 7.5 41.0 58-59 32.62668331856149 37.0 32.5 40.0 4.0 41.0 60-61 32.22181398222007 36.5 32.0 40.0 2.0 41.0 62-63 31.82426879381194 36.0 31.0 39.0 2.0 40.5 64-65 31.42772003330379 36.0 31.0 39.0 2.0 40.0 66-67 30.997996401042087 35.0 31.0 39.0 2.0 40.0 68-69 30.672508258802672 35.0 30.0 38.0 2.0 40.0 70-71 30.184368705180887 35.0 29.5 37.5 2.0 40.0 72-73 29.6587435876776 34.5 29.0 37.0 2.0 39.0 74-75 29.166881529825694 34.0 29.0 36.5 2.0 39.0 76-77 27.774275508285662 32.5 26.5 35.0 2.0 37.0 78-79 28.468431230360167 34.0 28.0 35.0 2.0 37.5 80-81 28.148892111836275 34.0 27.0 35.0 2.0 37.0 82-83 27.83482931807805 34.0 27.0 35.0 2.0 37.0 84-85 27.5007278489512 34.0 26.0 35.0 2.0 36.0 86-87 27.153017484489567 33.0 26.0 35.0 2.0 36.0 88-89 26.732417479117988 33.0 25.5 35.0 2.0 35.0 90-91 26.249238578680202 33.0 24.5 35.0 2.0 35.0 92-93 25.73811941020063 32.5 23.5 34.5 2.0 35.0 94-95 25.104179088443047 32.0 19.0 34.0 2.0 35.0 96-97 24.36582601455698 32.0 8.5 34.0 2.0 35.0 98-99 23.537926033357508 31.0 2.0 34.0 2.0 35.0 100-101 20.490951575215533 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18406231703032816 1101 2 0.24856096575501851 1101 3 0.22210038873046045 1101 4 0.23231916827597132 1101 5 0.20392116496817891 1101 6 0.21930188785703564 1101 7 0.24988547079816215 1101 8 0.16518742882673365 1101 9 0.334917671044046 1101 10-11 0.3655349459252051 1101 12-13 0.30067735093252423 1101 14-15 0.16372779990000907 1101 16-17 0.4434720612663625 1101 18-19 0.1579614997336165 1101 20-21 0.27859621116151345 1101 22-23 0.22801920445221668 1101 24-25 0.4830186043991418 1101 26-27 0.23020278977886477 1101 28-29 0.41011029827352985 1101 30-31 0.5427634052743926 1101 32-33 0.9014950254275789 1101 34-35 0.9256434563729314 1101 36-37 0.6689524533793829 1101 38-39 1.087544776690109 1101 40-41 1.3423823231195868 1101 42-43 1.3787825835460197 1101 44-45 1.5142156634886774 1101 46-47 1.9184758660736634 1101 48-49 1.9001667102349984 1101 50-51 1.8192325632957065 1101 52-53 2.338731766289712 1101 54-55 2.1127580573961566 1101 56-57 2.141740816266605 1101 58-59 2.2521105780225525 1101 60-61 2.687528371960081 1101 62-63 2.529337965253756 1101 64-65 2.560311510117174 1101 66-67 2.4071125316132687 1101 68-69 2.046519862404864 1101 70-71 2.3850949234060117 1101 72-73 2.496317119481006 1101 74-75 2.5152624712988185 1101 76-77 2.4843628541482055 1101 78-79 2.386271920909099 1101 80-81 2.7116052390850705 1101 82-83 2.689525314387172 1101 84-85 2.1931792850440424 1101 86-87 2.202397616843559 1101 88-89 1.9916186195794374 1101 90-91 2.5685930828903167 1101 92-93 2.3778239312360334 1101 94-95 2.2815902908299712 1101 96-97 2.1149986685965523 1101 98-99 2.042745522428941 1101 100-101 2.040642723771139 1102 1 0.26415542662989466 1102 2 0.31392622514211865 1102 3 0.2527961001777186 1102 4 0.24023783515534802 1102 5 0.2191421189790006 1102 6 0.26925765354571496 1102 7 0.2549397847312491 1102 8 0.1271966817744783 1102 9 0.255977554149446 1102 10-11 0.27382489095862894 1102 12-13 0.2683971809567467 1102 14-15 0.4258958612769277 1102 16-17 0.41172850424761265 1102 18-19 0.42323684019278573 1102 20-21 0.5314569383445829 1102 22-23 0.4667358293017472 1102 24-25 0.5202841988067561 1102 26-27 0.3833550283250844 1102 28-29 0.2754929613241046 1102 30-31 0.5980490429011169 1102 32-33 0.5160038795413655 1102 34-35 0.6196399176280281 1102 36-37 0.704535693651458 1102 38-39 0.7567337659723492 1102 40-41 1.001814383568309 1102 42-43 1.260996039478492 1102 44-45 1.2270624677409714 1102 46-47 1.0948764984067623 1102 48-49 1.0426229472728963 1102 50-51 1.0963568979681497 1102 52-53 0.8657799599344784 1102 54-55 0.9427931547841482 1102 56-57 0.7412390106365194 1102 58-59 0.912191650088225 1102 60-61 0.8633923913361592 1102 62-63 0.8800797964092162 1102 64-65 0.8055980179270712 1102 66-67 0.8489190318815787 1102 68-69 0.8035610945842322 1102 70-71 1.0439613647919224 1102 72-73 0.9544468057799733 1102 74-75 1.114651892772038 1102 76-77 0.9859364353870568 1102 78-79 1.0949817293237665 1102 80-81 1.2842619083139475 1102 82-83 1.5366377565562495 1102 84-85 1.1738213351956013 1102 86-87 0.746040928760717 1102 88-89 1.1702454705315652 1102 90-91 1.3585598708991888 1102 92-93 1.037179850657072 1102 94-95 1.049914898159816 1102 96-97 1.2919431654131337 1102 98-99 1.5113572902060568 1102 100-101 1.9019355259059871 1103 1 0.3297225312577119 1103 2 0.14237591043696085 1103 3 0.06664143296890046 1103 4 0.19711094501837323 1103 5 0.1283731879600012 1103 6 0.2080701604285622 1103 7 -0.3459564684560448 1103 8 -0.16775363462492265 1103 9 -0.13724717837234834 1103 10-11 -0.04176127387401607 1103 12-13 0.025532050966148745 1103 14-15 0.03548356786836848 1103 16-17 0.050757873905460826 1103 18-19 0.20019855883028015 1103 20-21 0.3383092937623431 1103 22-23 0.2659132755731477 1103 24-25 0.035502713466179614 1103 26-27 0.11928647935729941 1103 28-29 0.3649192068025968 1103 30-31 0.033180812450531505 1103 32-33 0.1955933650852586 1103 34-35 0.3928488937138255 1103 36-37 -0.022251094661669413 1103 38-39 0.07076122960971531 1103 40-41 0.12263913980947194 1103 42-43 0.31512278560503404 1103 44-45 0.36641797868152537 1103 46-47 0.33604315236662785 1103 48-49 0.562678518358986 1103 50-51 0.6835552172816435 1103 52-53 0.4406018529093103 1103 54-55 0.5991927244470858 1103 56-57 0.774263992211651 1103 58-59 0.7499130661343756 1103 60-61 1.002481059409046 1103 62-63 1.0311552155219452 1103 64-65 1.3199101139566167 1103 66-67 1.1401208413303578 1103 68-69 0.9895886937608331 1103 70-71 0.815310912006165 1103 72-73 1.1935888424263936 1103 74-75 1.1599219970525354 1103 76-77 1.0475263106062478 1103 78-79 1.066471898493674 1103 80-81 0.8000861139269624 1103 82-83 1.027433795422123 1103 84-85 1.2328105513552643 1103 86-87 1.0906367253502118 1103 88-89 1.2619165845793567 1103 90-91 1.3482334754804164 1103 92-93 1.5355364374402996 1103 94-95 1.713523460850947 1103 96-97 1.3006303832162125 1103 98-99 1.4390301662638976 1103 100-101 0.9325123300602662 1104 1 0.22199730676262774 1104 2 0.08636830299809617 1104 3 0.1659055429309717 1104 4 0.17039846082217736 1104 5 0.21569664459003235 1104 6 0.22138317844220268 1104 7 0.15761492152245182 1104 8 0.21688668696226898 1104 9 0.0843302845900169 1104 10-11 0.1802538329434995 1104 12-13 0.24537514753956202 1104 14-15 0.28498378939180213 1104 16-17 0.5072474298860499 1104 18-19 0.27089695197493313 1104 20-21 0.3087980500973657 1104 22-23 0.3990596647411877 1104 24-25 0.20207212594281287 1104 26-27 0.01718038584198922 1104 28-29 0.2542817596170295 1104 30-31 0.38078349666617584 1104 32-33 0.271835247061901 1104 34-35 0.414325860679817 1104 36-37 0.9324068407399295 1104 38-39 0.7031674063313886 1104 40-41 0.6083157588215826 1104 42-43 0.7315382122807819 1104 44-45 0.7361459911300656 1104 46-47 0.6420804763635601 1104 48-49 0.8653767818313938 1104 50-51 0.9529439206671739 1104 52-53 1.0223385966229088 1104 54-55 1.3223532695027913 1104 56-57 1.4894550966950675 1104 58-59 1.614324516194344 1104 60-61 1.5288045268451036 1104 62-63 1.772340203797409 1104 64-65 1.6126074048871288 1104 66-67 1.805517800516018 1104 68-69 1.8206905837339242 1104 70-71 1.7904840784945328 1104 72-73 1.4885932188649456 1104 74-75 1.5051797746862334 1104 76-77 1.3630837392054644 1104 78-79 1.396044736479002 1104 80-81 1.7966930232968643 1104 82-83 1.9743059926104003 1104 84-85 2.124723325152825 1104 86-87 1.5642970488824837 1104 88-89 1.8785351644520638 1104 90-91 1.7424317227437918 1104 92-93 1.2669743946700684 1104 94-95 0.8432011172410903 1104 96-97 1.2353620156818224 1104 98-99 1.1263255272390325 1104 100-101 0.700199085120186 1105 1 0.04976330597738965 1105 2 0.16998039565612544 1105 3 -0.15827192075179397 1105 4 0.10508240271460068 1105 5 0.19369428887743112 1105 6 0.15355436022468893 1105 7 0.26395178842469136 1105 8 0.11477439799485722 1105 9 0.19225333131164746 1105 10-11 0.31699509717593344 1105 12-13 0.2676523363891832 1105 14-15 0.1718546178174023 1105 16-17 0.13328590652523786 1105 18-19 -0.04891459101681761 1105 20-21 0.2597364089509284 1105 22-23 0.23039849473726548 1105 24-25 -0.10902328041760967 1105 26-27 0.4615698636590295 1105 28-29 0.4886908683724229 1105 30-31 0.32317061877061093 1105 32-33 0.395572977725422 1105 34-35 0.7434503208290053 1105 36-37 0.2550373864800193 1105 38-39 0.1867245323620068 1105 40-41 0.406431188739127 1105 42-43 0.5023195236274702 1105 44-45 0.38607139356430054 1105 46-47 0.6813030912045548 1105 48-49 1.3347682227422766 1105 50-51 0.9343809053550345 1105 52-53 0.967411231094836 1105 54-55 0.9431070975357656 1105 56-57 0.6732242368599657 1105 58-59 1.2843677042587345 1105 60-61 1.4230722928443136 1105 62-63 1.3049982328512009 1105 64-65 0.8265767806232844 1105 66-67 1.0351055508104814 1105 68-69 0.9516682044641982 1105 70-71 0.4569622881529547 1105 72-73 0.92465917881783 1105 74-75 0.9311554323226687 1105 76-77 0.5706455766612955 1105 78-79 0.7448001349870488 1105 80-81 0.8340310680554062 1105 82-83 0.8120209513854277 1105 84-85 0.7478564229148965 1105 86-87 0.7026244929343086 1105 88-89 0.5320491024183021 1105 90-91 0.7674179810869397 1105 92-93 0.7431816973791392 1105 94-95 0.9899148981598174 1105 96-97 1.0935559693528099 1105 98-99 1.2153400541334172 1105 100-101 1.7707997918339693 1106 1 0.15584517732241565 1106 2 0.22916168220583444 1106 3 0.31096784533007593 1106 4 -0.05117490722692253 1106 5 0.14351885028094102 1106 6 0.39086430174516096 1106 7 0.42114477088082936 1106 8 0.3886925266498267 1106 9 0.5703819862824062 1106 10-11 0.4404453895735969 1106 12-13 0.37075175159385765 1106 14-15 0.48600666460102815 1106 16-17 0.23264263167143184 1106 18-19 0.2793310230182726 1106 20-21 -0.1280998483590139 1106 22-23 0.011334167251888516 1106 24-25 0.14992408800343782 1106 26-27 0.21022483441927164 1106 28-29 0.5663516870858771 1106 30-31 0.6042817298817269 1106 32-33 0.326391691175715 1106 34-35 0.40842108106293296 1106 36-37 0.7476104858952226 1106 38-39 0.9416368130637665 1106 40-41 1.0608171536514064 1106 42-43 0.9187522721654773 1106 44-45 0.6206912766052355 1106 46-47 0.644460985941393 1106 48-49 1.1703237782978206 1106 50-51 1.7313984492146872 1106 52-53 1.8258907632585775 1106 54-55 1.190300079991907 1106 56-57 1.58182072808804 1106 58-59 1.5630226750189635 1106 60-61 1.2996804799788144 1106 62-63 1.5726005720324885 1106 64-65 0.863769763079425 1106 66-67 0.9244037964245173 1106 68-69 0.698159432534375 1106 70-71 0.9504710600827799 1106 72-73 0.7227293542564261 1106 74-75 0.5760092334922575 1106 76-77 0.6116982082402416 1106 78-79 0.9708820063320793 1106 80-81 1.0989433487571567 1106 82-83 1.064477091736304 1106 84-85 1.4738213351955984 1106 86-87 1.3943788788992215 1106 88-89 1.7130432544650809 1106 90-91 1.3959559927828487 1106 92-93 1.652830820186157 1106 94-95 1.6078096350019209 1106 96-97 0.9639653260779539 1106 98-99 1.569667539513535 1106 100-101 1.8371155813076534 1107 1 -0.033332823860313 1107 2 0.0917282108870836 1107 3 0.09571060109589524 1107 4 0.09057553629762083 1107 5 0.04552949486993896 1107 6 -0.09103989695383774 1107 7 0.1375847472261853 1107 8 0.30034243794492 1107 9 0.5517539555313746 1107 10-11 0.3797416639674438 1107 12-13 0.31800189943537305 1107 14-15 0.39703564153775517 1107 16-17 0.44769289778616184 1107 18-19 0.20168465991950768 1107 20-21 0.31208347511821444 1107 22-23 0.3813223399307759 1107 24-25 0.2822126746385649 1107 26-27 0.040059251923700856 1107 28-29 -0.11283222775740853 1107 30-31 0.44416937033116 1107 32-33 0.8340498815955826 1107 34-35 0.6276700461723337 1107 36-37 0.6115667248071119 1107 38-39 0.4842151690661325 1107 40-41 0.8622364321848224 1107 42-43 1.520496802029463 1107 44-45 1.0647480477465692 1107 46-47 1.3436626417663433 1107 48-49 2.0029080479607515 1107 50-51 2.195916485879387 1107 52-53 1.7813313309699907 1107 54-55 1.9134816293709704 1107 56-57 1.9591418398562297 1107 58-59 2.007463834096434 1107 60-61 2.069639084354929 1107 62-63 2.0004539132507055 1107 64-65 2.4144794023461316 1107 66-67 2.085255363544565 1107 68-69 2.2012936726289922 1107 70-71 1.9748649098758015 1107 72-73 2.093161051477008 1107 74-75 2.1447259691516294 1107 76-77 1.807499509245563 1107 78-79 1.6052995107673205 1107 80-81 1.74376889577076 1107 82-83 2.0245303146813107 1107 84-85 2.2082684079336268 1107 86-87 1.807418500424948 1107 88-89 1.6877919238914565 1107 90-91 2.09997728196085 1107 92-93 2.008537502622584 1107 94-95 2.2549710779350995 1107 96-97 1.443356219040698 1107 98-99 1.0034049729848569 1107 100-101 0.7388896794744149 1108 1 -0.005636552479796109 1108 2 -0.11653057391217203 1108 3 -0.3219520339860473 1108 4 -0.2477555307602799 1108 5 -0.08715496802278011 1108 6 -0.44443573177814244 1108 7 -0.20976371051374088 1108 8 -0.2657493104269406 1108 9 -0.16763343443498968 1108 10-11 -0.40248119440227015 1108 12-13 -0.2188727874607821 1108 14-15 -0.10269598373956512 1108 16-17 -0.21390446856322853 1108 18-19 -0.02645174600620237 1108 20-21 -0.13068114235834116 1108 22-23 -0.13526321793081308 1108 24-25 -0.2767939810545599 1108 26-27 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0.03416143995031007 2316 54-55 0.2575933130746151 2316 56-57 0.5002417807196196 2316 58-59 0.4739249306580646 2316 60-61 0.25456769802393 2316 62-63 0.5350065870700824 2316 64-65 0.37880735706438884 2316 66-67 -0.06995710583112569 2316 68-69 -0.5375548531799126 2316 70-71 -0.23561916548112904 2316 72-73 -0.03328568333755655 2316 74-75 -0.1544418943272916 2316 76-77 0.21921700523272492 2316 78-79 0.011483510091476745 2316 80-81 -0.049928831693968334 2316 82-83 0.15733423459344564 2316 84-85 -0.0028703941277079537 2316 86-87 -0.1405835271158189 2316 88-89 -0.41740787335446683 2316 90-91 -0.2205853606006123 2316 92-93 0.4517030006372842 2316 94-95 -0.1117392371785293 2316 96-97 -0.2864106137716682 2316 98-99 -0.7397309567270689 2316 100-101 -0.6425836668126514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 7.0 6 16.0 7 42.0 8 90.0 9 162.0 10 358.0 11 565.0 12 812.0 13 1229.0 14 1523.0 15 2127.0 16 2962.0 17 3802.0 18 3245.0 19 1809.0 20 1556.0 21 1565.0 22 1607.0 23 1617.0 24 1899.0 25 2080.0 26 2315.0 27 2708.0 28 3319.0 29 4112.0 30 4865.0 31 6037.0 32 7541.0 33 9621.0 34 13148.0 35 18355.0 36 26018.0 37 35690.0 38 22284.0 39 1075.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04005587942938 21.030008328184188 16.323241006904333 19.606694785482095 2 15.695406929895245 23.97904915390814 39.01477303250067 21.310770883695945 3 19.51494641849972 26.871323825638548 32.86117154137459 20.75255821448715 4 12.498589960518895 20.096151263663952 38.07160314774528 29.33365562807187 5 11.950151747106062 39.26785378562028 34.5779281819891 14.204066285284558 6 27.042677194961456 39.312437891118094 17.963634410334915 15.681250503585527 7 24.095291811027852 37.05530040555421 21.538957376520827 17.310450406897107 8 24.669513603523754 33.78669459887734 20.90511105739532 20.638680740203583 9 25.116966132194555 18.18601777992641 22.455348749764994 34.24166733811404 10-11 23.688776908076047 27.956463579076303 27.793148278474078 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19.756966268594176 28.042085018829205 33.67929646999415 18.521652242582476 38-39 19.473029636374868 28.763642322021926 33.262687393057355 18.50064064854585 40-41 19.371733373441554 28.984008637591792 32.91980683594484 18.724451153021814 42-43 19.228909838046892 29.144038890231784 32.22759380119786 19.39945747052346 44-45 20.085408105712673 28.96543388929176 31.59052453468697 19.358633470308597 46-47 20.628316967834813 29.088867879933822 31.206892847167012 19.075922305064353 48-49 20.328644676445073 29.163320503764268 31.02759207879307 19.480442740997592 50-51 19.937631947913815 29.71668466261623 30.84425177139235 19.501431618077604 52-53 20.47672763408804 30.223726264335397 29.67367657722988 19.62586952434668 54-55 19.868929952728838 29.373925655350234 30.01745810055866 20.73968629136227 56-57 20.6197307176833 30.070127281956793 29.357842518458437 19.952299481901466 58-59 20.841726425482772 29.55872478715118 29.444310154970054 20.155238632395992 60-61 20.13455986033707 29.16537970858793 30.982340696971733 19.71771973410327 62-63 19.923988074454083 29.14399290913486 30.616421799038434 20.31559721737262 64-65 19.777079472510945 29.826766578035613 30.518894529046815 19.877259420406627 66-67 19.90180437153186 29.35796818848404 29.838471414221175 20.901756025762925 68-69 19.696774366825128 29.324523943813286 30.054252947653964 20.924448741707625 70-71 20.514493979754356 29.39915719246791 29.684658915415753 20.401689912361984 72-73 20.01584615797814 28.883248730964468 30.179410737786373 20.921494373271024 74-75 19.44914457604813 30.27636773829667 29.395160207342947 20.879327478312252 76-77 19.73356968280826 29.361856417693982 29.448607418150562 21.45596648134719 78-79 19.5629577703111 29.497664649590007 29.41064296668735 21.528734613411547 80-81 19.648204899561936 29.23997969483158 29.119384183992757 21.992431221613725 82-83 19.41021777029223 29.248159280957793 29.630942883314127 21.71068006543585 84-85 19.28443841577244 29.385836500624507 29.195396124041416 22.13432895956164 86-87 19.0419145592995 29.312257423349138 28.65768657417977 22.988141443171592 88-89 19.263825101388555 29.007869363199312 28.52308436064781 23.205221174764322 90-91 19.47959863346297 28.49799101867171 28.938194280311986 23.08421606755334 92-93 19.34681599656219 29.13410146912685 28.811269572690897 22.707812961620068 94-95 19.2243439959176 29.459350576101844 28.216635780087557 23.099669647893 96-97 19.281810246424495 29.275498556368763 28.019069361444977 23.423621835761768 98-99 19.880318970356385 29.402427462176657 26.920442733862803 23.796810833604155 100-101 19.400315312532733 28.754139484165886 27.27265402372633 24.572891179575056 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1303.0 1 837.5 2 358.0 3 319.5 4 291.0 5 301.5 6 390.0 7 612.0 8 858.0 9 912.0 10 764.0 11 639.0 12 585.0 13 557.5 14 560.5 15 569.0 16 562.0 17 571.5 18 609.0 19 643.0 20 684.0 21 760.5 22 892.0 23 1050.0 24 1239.5 25 1467.5 26 1774.5 27 2255.0 28 2668.5 29 3102.0 30 3345.5 31 3572.0 32 3956.5 33 4201.5 34 4723.5 35 5375.5 36 5885.0 37 5947.5 38 5599.5 39 5299.5 40 5324.5 41 5427.5 42 5446.0 43 5504.5 44 5632.0 45 5636.5 46 5710.5 47 6794.5 48 9258.0 49 8807.5 50 6089.5 51 5215.0 52 4755.0 53 4239.0 54 3881.5 55 3612.0 56 3282.5 57 2919.0 58 2673.0 59 2418.5 60 2031.5 61 1622.0 62 1266.0 63 928.0 64 662.0 65 503.0 66 356.0 67 235.0 68 156.0 69 114.5 70 78.5 71 49.0 72 38.5 73 34.5 74 26.0 75 20.5 76 13.5 77 5.5 78 3.5 79 2.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026857894878199447 2 0.008057368463459834 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011817473746407757 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.685789487819945E-4 22-23 0.002148631590255956 24-25 0.010474579002497784 26-27 0.004028684231729917 28-29 0.0 30-31 0.0026857894878199447 32-33 0.0024172105390379505 34-35 0.0 36-37 0.009668842156151802 38-39 0.01369752638788172 40-41 0.001074315795127978 42-43 0.0 44-45 0.0 46-47 5.37157897563989E-4 48-49 0.0034915263341659284 50-51 0.006445894770767868 52-53 0.0 54-55 0.0026857894878199447 56-57 0.001880052641473961 58-59 0.0 60-61 0.0013428947439099723 62-63 0.005371578975639889 64-65 0.0 66-67 0.003222947385383934 68-69 0.0 70-71 8.057368463459835E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0024172105390379505 80-81 0.0029543684366019392 82-83 0.015309000080573685 84-85 0.009400263207369806 86-87 0.006714473719549862 88-89 0.0 90-91 5.37157897563989E-4 92-93 0.0 94-95 0.0 96-97 0.0013428947439099723 98-99 0.001880052641473961 100-101 2.685789487819945E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 186165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.98405002065099 #Duplication Level Percentage of deduplicated Percentage of total 1 81.208226710668 48.71198333101075 2 12.174816681577184 14.605896256399639 3 3.224519702242217 5.802592533356153 4 1.1921152604142327 2.8603160564427488 5 0.6054316755899061 1.8158121956335735 6 0.3026606400179531 1.0892886582071488 7 0.22300553053954103 0.9363742429135948 8 0.1353953588107363 0.6497249580373757 9 0.1204619053294834 0.6503213659379992 >10 0.6849402405925256 7.936426681597991 >50 0.06670202878001687 2.7469949286222977 >100 0.05575094942807379 6.734914957609191 >500 0.0 0.0 >1k 0.005973316010150764 5.459353834231532 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1935 1.0394005317863186 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1718 0.9228372680149329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1598 0.8583783203072543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1496 0.8035882147557275 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 0.6859506351892138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1118 0.6005425294765396 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 500 0.26857894878199445 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 465 0.24977842236725487 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 442 0.2374237907232831 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 429 0.23044073805495124 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 379 0.2035828431767518 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 350 0.18800526414739613 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 347 0.18639379045470417 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 333 0.1788735798888083 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 326 0.17511347460586038 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 313 0.16813042193752853 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 277 0.14879273762522494 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 275 0.14771842183009695 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 268 0.14395831654714905 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 247 0.13267800069830527 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 241 0.12945505331292134 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 215 0.11548894797625761 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 213 0.11441463218112963 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 211 0.11334031638600167 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 187 0.10044852684446594 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.685789487819945E-4 0.0 12-13 0.0 0.0 0.0 5.37157897563989E-4 0.0 14-15 0.0 0.0 0.0 5.37157897563989E-4 0.0 16-17 0.0 0.0 0.0 5.37157897563989E-4 0.0 18-19 0.0 0.0 0.0 5.37157897563989E-4 0.0 20-21 0.0 0.0 0.0 0.001074315795127978 0.0 22-23 0.0 0.0 0.0 0.0018800526414739613 0.0 24-25 0.0 0.0 0.0 0.0032229473853839334 0.0 26-27 0.0 0.0 0.0 0.004565842129293906 0.0 28-29 0.0 0.0 0.0 0.01262321059275374 0.0 30-31 0.0 0.0 0.0 0.024977842236725487 0.0 32-33 0.0 0.0 0.0 0.035989579136787256 0.0 34-35 0.0 0.0 0.0 0.053715789756398895 0.0 36-37 0.0 0.0 0.0 0.06902478983697258 0.0 38-39 0.0 0.0 0.0 0.08218515832729031 0.0 40-41 0.0 0.0 0.0 0.09749415840786399 0.0 42-43 0.0 0.0 0.0 0.14261542180323905 0.0 44-45 0.0 0.0 0.0 0.21942900115488947 0.0 46-47 0.0 0.0 0.0 0.291408159428464 0.0 48-49 0.0 0.0 0.0 0.4224746864340773 0.0 50-51 0.0 0.0 0.0 0.5511240029006527 0.0 52-53 0.0 0.0 0.0 0.7007224773722236 0.0 54-55 0.0 0.0 0.0 0.921225794322241 0.0 56-57 0.0 0.0 0.0 1.1881932694115434 0.0 58-59 0.0 0.0 0.0 1.462680955066742 0.0 60-61 0.0 0.0 0.0 1.7836327988612253 0.0 62-63 0.0 0.0 0.0 2.2028845379099184 0.0 64-65 0.0 0.0 0.0 2.570837697741251 0.0 66-67 0.0 0.0 0.0 3.02715333172186 0.0 68-69 0.0 0.0 0.0 3.609432492681224 0.0 70-71 0.0 0.0 0.0 4.091800284693686 0.0 72-73 0.0 0.0 0.0 4.673005129857922 0.0 74-75 0.0 0.0 0.0 5.349286922890983 0.0 76-77 0.0 0.0 0.0 5.976150189348159 0.0 78-79 0.0 0.0 0.0 6.5922702978540535 0.0 80-81 0.0 0.0 0.0 7.361480407165686 0.0 82-83 0.0 0.0 0.0 8.286466306770876 0.0 84-85 0.0 0.0 0.0 9.257379206617784 0.0 86-87 0.0 0.0 0.0 10.211640211640212 0.0 88-89 0.0 0.0 0.0 11.224988585394676 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAT 50 0.0016062082 38.02946 1 GCCTGAT 70 2.0471256E-4 33.927517 7 ATAAGGT 60 0.0039431257 31.665682 3 TATTATA 120 5.34008E-6 27.714914 2 GTGCACT 70 0.008375103 27.142014 4 AAACTAT 90 8.9209125E-4 26.38807 6 TCTAAAC 150 1.0524273E-6 25.332546 3 AACTTAC 135 1.3352024E-5 24.628864 7 AATCTAA 140 1.7585204E-5 23.768414 1 AGTACTT 1595 0.0 22.930323 12-13 CGGTGTT 110 0.0028689322 21.590237 4 GAGTACT 1545 0.0 21.36665 12-13 CATGGGT 160 4.961717E-5 20.780603 4 ACTTACT 160 4.961717E-5 20.780603 8 GTACATG 945 0.0 20.624443 1 TACATGG 965 0.0 20.186142 2 ATCTAAA 165 6.274883E-5 20.156303 2 ATGTCCA 120 0.004749645 19.79105 4 CTTACTT 170 7.910153E-5 19.558216 9 CTAAACT 245 1.7369348E-7 19.387152 4 >>END_MODULE