##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936796_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 196191 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.930363778154963 33.0 31.0 34.0 30.0 34.0 2 32.009658954794055 33.0 31.0 34.0 30.0 34.0 3 31.984703681616384 33.0 31.0 34.0 30.0 34.0 4 35.61446243711485 37.0 35.0 37.0 33.0 37.0 5 35.61761752577845 37.0 35.0 37.0 33.0 37.0 6 35.70665321039191 37.0 35.0 37.0 35.0 37.0 7 35.688757384385625 37.0 35.0 37.0 35.0 37.0 8 35.660116926872284 37.0 35.0 37.0 33.0 37.0 9 37.29938172495171 39.0 37.0 39.0 34.0 39.0 10-11 37.201497520273605 39.0 37.0 39.0 34.0 39.0 12-13 37.134916994153656 39.0 37.0 39.0 34.0 39.0 14-15 38.26623800276262 40.0 38.0 41.0 33.5 41.0 16-17 38.21781835048499 40.0 38.0 41.0 33.5 41.0 18-19 38.22631517245949 40.0 38.0 41.0 34.0 41.0 20-21 38.211006111391455 40.0 38.0 41.0 34.0 41.0 22-23 38.10234669276369 40.0 38.0 41.0 34.0 41.0 24-25 38.03275634458257 40.0 38.0 41.0 33.5 41.0 26-27 37.96559220351597 40.0 38.0 41.0 33.5 41.0 28-29 37.86187949498193 40.0 37.0 41.0 33.0 41.0 30-31 37.62051266367978 40.0 37.0 41.0 33.0 41.0 32-33 37.29121876130913 39.5 35.5 41.0 33.0 41.0 34-35 37.06094316253039 39.0 35.0 41.0 32.5 41.0 36-37 36.81948713243727 39.0 35.0 41.0 31.5 41.0 38-39 36.599018303591905 39.0 35.0 41.0 31.0 41.0 40-41 36.340956007156294 39.0 35.0 41.0 31.0 41.0 42-43 35.997920393901865 39.0 35.0 41.0 30.0 41.0 44-45 35.55590215657192 39.0 35.0 40.5 28.5 41.0 46-47 34.93091935919588 38.5 35.0 40.0 25.0 41.0 48-49 34.436798833789524 38.5 35.0 40.0 21.5 41.0 50-51 33.61598391363518 38.0 34.0 39.5 16.5 40.5 52-53 33.76075355138615 38.0 34.5 40.0 15.0 40.5 54-55 34.1016331024359 39.0 35.0 40.5 13.5 41.0 56-57 33.96274293927856 38.5 34.5 41.0 8.5 41.0 58-59 33.735994515548626 38.0 34.0 40.5 6.5 41.0 60-61 33.414593431910745 38.0 34.0 40.0 2.0 41.0 62-63 33.10374838805042 38.0 34.0 40.0 2.0 41.0 64-65 32.76086823554597 37.0 33.0 40.0 2.0 41.0 66-67 32.36675739457978 36.5 33.0 39.5 2.0 41.0 68-69 31.956045384344847 36.0 32.5 39.0 2.0 41.0 70-71 31.510905189330806 35.5 32.0 39.0 2.0 40.5 72-73 31.113045450606805 35.0 31.5 38.5 2.0 40.0 74-75 30.735953738958464 35.0 31.0 37.0 2.0 40.0 76-77 30.282795846904293 35.0 31.0 37.0 2.0 39.0 78-79 29.826775947928294 35.0 30.5 36.0 2.0 39.0 80-81 29.39805342752726 35.0 30.0 36.0 2.0 38.5 82-83 28.991133640177175 34.5 29.5 35.5 2.0 37.0 84-85 28.603386495812757 34.0 29.0 35.0 2.0 37.0 86-87 28.251415712239606 34.0 29.0 35.0 2.0 36.0 88-89 27.84002833973016 34.0 28.5 35.0 2.0 36.0 90-91 27.442713987899545 34.0 27.0 35.0 2.0 36.0 92-93 27.04554745120826 34.0 26.0 35.0 2.0 35.0 94-95 26.6669495542609 34.0 25.0 35.0 2.0 35.0 96-97 26.19472860630712 34.0 24.5 35.0 2.0 35.0 98-99 25.70396705251515 34.0 21.0 35.0 2.0 35.0 100-101 23.83548939553802 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41980452815307245 1101 2 0.15461667934264156 1101 3 0.23967566749954372 1101 4 0.17934224571538238 1101 5 0.15731730748098016 1101 6 0.2375652339514076 1101 7 0.3785636645785928 1101 8 0.3224928678601344 1101 9 0.20815600461767048 1101 10-11 0.25110968052057103 1101 12-13 0.43218441089415904 1101 14-15 0.6194615746872856 1101 16-17 0.4060324991359181 1101 18-19 0.6566586218980959 1101 20-21 0.5752346549044134 1101 22-23 0.5656094478542997 1101 24-25 0.6339838917862508 1101 26-27 0.5548012742056514 1101 28-29 0.29853559417274766 1101 30-31 0.49250256839174966 1101 32-33 0.6524358560729269 1101 34-35 0.7355184561641934 1101 36-37 0.966692237639414 1101 38-39 0.5700288364964408 1101 40-41 0.5806763329182019 1101 42-43 0.4762546790874751 1101 44-45 1.0251049112994508 1101 46-47 1.3590726494278655 1101 48-49 1.300168279391464 1101 50-51 1.8730735084178107 1101 52-53 1.3825266137203798 1101 54-55 1.3464020109739039 1101 56-57 1.3644107563132692 1101 58-59 1.1757671483892764 1101 60-61 1.2690461831238906 1101 62-63 0.8036817529765088 1101 64-65 1.1874147213174027 1101 66-67 1.1068391302203402 1101 68-69 1.092650158808791 1101 70-71 1.0391354152106196 1101 72-73 1.2782963248382337 1101 74-75 1.2246199168457075 1101 76-77 1.1219880679248213 1101 78-79 1.1639538348484848 1101 80-81 1.3680102993155572 1101 82-83 1.2326872405869054 1101 84-85 0.8413751435605228 1101 86-87 0.9695471767873904 1101 88-89 1.0250112535959026 1101 90-91 1.1487672527915613 1101 92-93 1.1237530075877906 1101 94-95 1.5610296178440386 1101 96-97 1.60435567015789 1101 98-99 1.6002840821664854 1101 100-101 2.0447015510866358 1102 1 0.16747466552984847 1102 2 0.31582654513305286 1102 3 0.4831532398499938 1102 4 0.2399226329041113 1102 5 0.3528911623445907 1102 6 0.25442259172486814 1102 7 0.192150900811221 1102 8 0.11218367113816896 1102 9 0.3131086947479247 1102 10-11 0.03663167268178569 1102 12-13 0.22188709112694482 1102 14-15 0.30063264242551924 1102 16-17 0.43354744430396863 1102 18-19 0.020592269310895972 1102 20-21 -0.06518658109067843 1102 22-23 0.21254321403664278 1102 24-25 0.4148976255049277 1102 26-27 0.12588484837723257 1102 28-29 0.26171703445146477 1102 30-31 0.39941495605555133 1102 32-33 0.5048924062134716 1102 34-35 0.4827887360644283 1102 36-37 0.03966424761605225 1102 38-39 0.5534010998481165 1102 40-41 0.7130449935936056 1102 42-43 0.5469344367975921 1102 44-45 1.0496981255992992 1102 46-47 1.101453582956509 1102 48-49 1.288449684039314 1102 50-51 1.4058657804792531 1102 52-53 1.7473906225885045 1102 54-55 1.40754457402074 1102 56-57 1.992658113690844 1102 58-59 1.7158320757419077 1102 60-61 1.468903659666907 1102 62-63 1.573929981330764 1102 64-65 1.5828777246033567 1102 66-67 1.359691578852516 1102 68-69 1.5501702506572421 1102 70-71 1.3691405618910117 1102 72-73 1.4414262983130364 1102 74-75 1.744818657888505 1102 76-77 1.7771406867933415 1102 78-79 1.7662540050848357 1102 80-81 1.3436902549749306 1102 82-83 1.2352091139785628 1102 84-85 1.4968761531016774 1102 86-87 1.6148617181389895 1102 88-89 1.848155479416402 1102 90-91 1.6741522640746673 1102 92-93 1.2659082789762053 1102 94-95 1.148475676674554 1102 96-97 1.1913147738437075 1102 98-99 1.5245605776730358 1102 100-101 1.571662238366418 1103 1 0.0931133433066087 1103 2 0.13141119855307082 1103 3 0.2774944173978362 1103 4 0.139904319562838 1103 5 0.21993630473082248 1103 6 0.13349044265427068 1103 7 0.33942679129744135 1103 8 0.15318724223971714 1103 9 0.0856112128323474 1103 10-11 0.2090517807305048 1103 12-13 0.15651761946683962 1103 14-15 0.3779561475990434 1103 16-17 0.5389636149843113 1103 18-19 0.4243190342591632 1103 20-21 0.17992375679046546 1103 22-23 0.371654101318029 1103 24-25 0.24809513489051227 1103 26-27 0.4832239198908326 1103 28-29 0.4307262826888021 1103 30-31 0.2728194061974847 1103 32-33 0.37170405349851876 1103 34-35 0.4441521643219204 1103 36-37 0.4836895200270064 1103 38-39 0.5232069784306645 1103 40-41 0.729961942590954 1103 42-43 0.6267851830662536 1103 44-45 0.7477294414128721 1103 46-47 0.809085667930411 1103 48-49 0.8403222231840815 1103 50-51 1.050385513104473 1103 52-53 1.0153926370560455 1103 54-55 1.1626173695522866 1103 56-57 1.2783828641715687 1103 58-59 1.2312381740845453 1103 60-61 1.2953801728067234 1103 62-63 1.6387088477349394 1103 64-65 1.433944476732286 1103 66-67 1.3456635594403714 1103 68-69 1.6005548308238922 1103 70-71 1.5414095848742555 1103 72-73 1.3095244578222385 1103 74-75 1.2287899059427119 1103 76-77 1.2892961670607157 1103 78-79 1.06789762746374 1103 80-81 0.9490789556433654 1103 82-83 1.018809516872075 1103 84-85 1.1484694137920926 1103 86-87 0.8922172716597281 1103 88-89 0.8141888096975123 1103 90-91 0.6589705041625713 1103 92-93 0.965793820593273 1103 94-95 1.3927573358632763 1103 96-97 1.3873819838061614 1103 98-99 1.2318035041449704 1103 100-101 1.5864502614412253 1104 1 0.025881555809633028 1104 2 0.12120823881518206 1104 3 -0.11760640279698364 1104 4 0.3538476315667083 1104 5 0.16522733096386588 1104 6 -0.21460859642042607 1104 7 -0.024229562463801813 1104 8 0.04629804550290828 1104 9 0.2811716059981251 1104 10-11 0.11377284852301273 1104 12-13 -0.02139649096260854 1104 14-15 -0.31560873135075695 1104 16-17 0.09901045184690815 1104 18-19 0.05979410957196052 1104 20-21 0.1585228288488736 1104 22-23 0.020877884769006982 1104 24-25 0.3349907084771573 1104 26-27 0.29517281713558674 1104 28-29 -0.06805881747171583 1104 30-31 0.1211878122049157 1104 32-33 -0.41445708290009975 1104 34-35 -0.23598834626585585 1104 36-37 -0.495530745161993 1104 38-39 -0.05361757024228808 1104 40-41 -0.5215882915933605 1104 42-43 -0.3956162506255012 1104 44-45 -0.4854433712176913 1104 46-47 -0.7008039466000042 1104 48-49 -0.8610982750897662 1104 50-51 -0.845089122425577 1104 52-53 -0.8114923817446638 1104 54-55 -0.358640628996433 1104 56-57 -0.27807424601599706 1104 58-59 -0.7328920455869294 1104 60-61 -0.3066219381504567 1104 62-63 -0.57716401107281 1104 64-65 -0.7016405442673417 1104 66-67 -0.5336160764195093 1104 68-69 -0.5759464775297793 1104 70-71 -0.7149122921185089 1104 72-73 -0.6051686837658181 1104 74-75 -0.298240241164617 1104 76-77 -0.02516498912812537 1104 78-79 -0.28539634531370695 1104 80-81 -0.27838217855634184 1104 82-83 -0.42810870552606417 1104 84-85 -0.32750195635105683 1104 86-87 -0.2093505538344793 1104 88-89 -0.44479375291655643 1104 90-91 -0.8655363894319912 1104 92-93 -0.6249278628950812 1104 94-95 -0.4006657131505129 1104 96-97 -0.09347097483226818 1104 98-99 -0.3119878629197004 1104 100-101 -0.03030106340429839 1105 1 0.43640382923513243 1105 2 0.5830617974429337 1105 3 0.8016204277201702 1105 4 0.5810621732973829 1105 5 0.6013512428942249 1105 6 0.3729490072662145 1105 7 0.27812578827865053 1105 8 0.34122892107894387 1105 9 0.5416682777851207 1105 10-11 0.30111283828235 1105 12-13 0.5361431915770751 1105 14-15 0.8113113915371102 1105 16-17 0.9325998015652885 1105 18-19 0.8471084976927941 1105 20-21 0.8301818150239839 1105 22-23 0.6853044080665001 1105 24-25 0.5382037141095211 1105 26-27 0.8985394325989873 1105 28-29 1.026731249092542 1105 30-31 1.0623680477399944 1105 32-33 1.0673657537614432 1105 34-35 0.8346721760075724 1105 36-37 0.8548916818733403 1105 38-39 1.291940545477118 1105 40-41 1.302656460070743 1105 42-43 1.5077375640186332 1105 44-45 1.457913003590292 1105 46-47 1.691669171679564 1105 48-49 1.6357015200971219 1105 50-51 2.024214513007088 1105 52-53 2.2401589239388997 1105 54-55 2.0868918850844693 1105 56-57 2.5716441359604474 1105 58-59 2.2530270532353924 1105 60-61 2.4467702789514334 1105 62-63 2.261225645865231 1105 64-65 2.499396322092103 1105 66-67 2.7110715835907264 1105 68-69 2.4989383150969466 1105 70-71 2.444649919863057 1105 72-73 2.1477755046622384 1105 74-75 2.12303472043293 1105 76-77 1.9975564650449407 1105 78-79 1.8301310484394904 1105 80-81 2.1810392244134533 1105 82-83 1.797889758375625 1105 84-85 2.16654566269656 1105 86-87 2.487078017594097 1105 88-89 2.2650116746308058 1105 90-91 1.9224820437477312 1105 92-93 2.495847866751614 1105 94-95 2.391320973997466 1105 96-97 2.5159120256797642 1105 98-99 2.5614120346737472 1105 100-101 2.510213529529647 1106 1 -0.006746619350099792 1106 2 -0.19747650483449775 1106 3 -0.2908571430182718 1106 4 -0.2313946935073119 1106 5 -0.356550758486037 1106 6 -0.3917162791382012 1106 7 -0.6032579246268028 1106 8 -0.1496268415159463 1106 9 -0.37637865113137536 1106 10-11 -0.4373705985706451 1106 12-13 -0.20889649096260854 1106 14-15 -0.2471772212993315 1106 16-17 0.11423368700351944 1106 18-19 -0.040788327704028404 1106 20-21 -0.5710431677227348 1106 22-23 -0.3962173310639372 1106 24-25 -0.3970102265392015 1106 26-27 -0.5094574618109604 1106 28-29 -0.11198510701511566 1106 30-31 -0.2342158031916881 1106 32-33 -0.047541078536688985 1106 34-35 0.3542115134816868 1106 36-37 0.5131473754551195 1106 38-39 0.17004613553923065 1106 40-41 0.6331861357091171 1106 42-43 0.8767851830662536 1106 44-45 0.7733029276758714 1106 46-47 0.9039276257858404 1106 48-49 1.4686897878680014 1106 50-51 1.77339498229626 1106 52-53 1.197974658928544 1106 54-55 1.7678787725930931 1106 56-57 1.5491717412054484 1106 58-59 1.4615329193499562 1106 60-61 2.171580492892076 1106 62-63 1.9672297866519841 1106 64-65 1.5349380750251598 1106 66-67 1.4442065042256509 1106 68-69 1.6967471487753478 1106 70-71 1.513835565135654 1106 72-73 1.419687167878255 1106 74-75 1.4667200206399649 1106 76-77 1.6085813097654373 1106 78-79 1.6745927461620589 1106 80-81 1.8233787689269079 1106 82-83 2.1047824429857016 1106 84-85 1.992244420460537 1106 86-87 1.5581615234602104 1106 88-89 1.7261486656591032 1106 90-91 2.1482843211804443 1106 92-93 2.166914119339605 1106 94-95 2.5443144177517816 1106 96-97 2.332984144382319 1106 98-99 2.2436066516509747 1106 100-101 2.706598967762911 1107 1 0.07513503915839337 1107 2 0.17309648681537126 1107 3 0.1614175474510624 1107 4 -0.09966420113760677 1107 5 0.3167144301117162 1107 6 0.2470686330414651 1107 7 0.24086099273570483 1107 8 0.007369991193520775 1107 9 0.29235155330282936 1107 10-11 0.25329669195627247 1107 12-13 0.5183386083751387 1107 14-15 0.2032618803418984 1107 16-17 0.05443308233896005 1107 18-19 0.26757259944829315 1107 20-21 0.3492346900371288 1107 22-23 0.8417613617088833 1107 24-25 0.25854692865802775 1107 26-27 -0.1959496875523925 1107 28-29 -0.3690035348872698 1107 30-31 0.15496502030384818 1107 32-33 0.3436193015381761 1107 34-35 0.09973051722485593 1107 36-37 0.09184374746778445 1107 38-39 0.12649589079405388 1107 40-41 0.13817101909523188 1107 42-43 -0.17288369110593038 1107 44-45 -0.5373434875271244 1107 46-47 -0.8167929328090366 1107 48-49 -0.52720713122212 1107 50-51 -0.7837062270328516 1107 52-53 -0.9539833024880977 1107 54-55 -0.8924653103323337 1107 56-57 -0.46362339268456054 1107 58-59 -0.5140285548798218 1107 60-61 -0.8442919516803329 1107 62-63 -1.5079717681997309 1107 64-65 -1.358705748758311 1107 66-67 -1.3161411778935523 1107 68-69 -1.370161863605876 1107 70-71 -1.8270440778243184 1107 72-73 -1.3499644301637836 1107 74-75 -1.4295512146033396 1107 76-77 -1.2161379531196737 1107 78-79 -1.3372845933138962 1107 80-81 -1.244142106397021 1107 82-83 -1.1058603730235106 1107 84-85 -0.8617182918918971 1107 86-87 -1.1372387068881942 1107 88-89 -0.6720481382568231 1107 90-91 -0.5724722293522397 1107 92-93 -0.30220479802290257 1107 94-95 -0.234533120399675 1107 96-97 -0.19985849886559492 1107 98-99 -0.023813446247100245 1107 100-101 -4.874314984348871E-5 1108 1 0.2913100341413326 1108 2 0.20990162428276093 1108 3 -0.0815686054688669 1108 4 0.4143233565585618 1108 5 0.4475994391424223 1108 6 0.4429345772517834 1108 7 0.27971308986595034 1108 8 0.47744825729827767 1108 9 0.22428011039694695 1108 10-11 0.25684876901829057 1108 12-13 0.0513307817646691 1108 14-15 0.1826317378574558 1108 16-17 0.08180615077164077 1108 18-19 -0.1548034792191757 1108 20-21 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0.27294929605834994 2312 20-21 0.534370090994436 2312 22-23 0.5008984674724388 2312 24-25 0.9612549478043135 2312 26-27 1.1833697013401405 2312 28-29 1.1827307776168254 2312 30-31 0.8744260392534215 2312 32-33 0.39692728134277644 2312 34-35 0.6166146559698547 2312 36-37 0.9115277026182653 2312 38-39 1.1184964153498171 2312 40-41 0.9099564757866005 2312 42-43 1.1936168662345708 2312 44-45 1.336959836845054 2312 46-47 0.6845184566080533 2312 48-49 0.12678277107936964 2312 50-51 -0.34711190534903125 2312 52-53 -0.7072125989276756 2312 54-55 -1.1221879514706146 2312 56-57 -1.3855301528970116 2312 58-59 -1.5160284237408774 2312 60-61 -1.0339188613911716 2312 62-63 -0.7917564380574476 2312 64-65 -1.336510482874104 2312 66-67 -1.1838875551802879 2312 68-69 -1.2858723088225865 2312 70-71 -1.7927184598320558 2312 72-73 -1.6526331076275582 2312 74-75 -2.045871455898993 2312 76-77 -2.182959800867362 2312 78-79 -2.5136336851767282 2312 80-81 -1.891214429523373 2312 82-83 -1.7519491540870469 2312 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0.19145513586256868 2314 62-63 0.19140465177416388 2314 64-65 0.46135032510449747 2314 66-67 0.745757599116164 2314 68-69 0.49804038901654124 2314 70-71 0.8473408015685351 2314 72-73 0.6823022107217973 2314 74-75 1.153882826098549 2314 76-77 1.3331925442941603 2314 78-79 1.4661668496212812 2314 80-81 0.8827250082160205 2314 82-83 0.9285090377175358 2314 84-85 0.8342985302669454 2314 86-87 0.7111685053707113 2314 88-89 0.7816667551545002 2314 90-91 0.7808414459027553 2314 92-93 0.3285294795427163 2314 94-95 0.0863447255905605 2314 96-97 0.3496653566958692 2314 98-99 0.23876691823300789 2314 100-101 -0.33666110840338703 2315 1 -0.6824712570312599 2315 2 -1.0380562149794237 2315 3 -0.533610766206678 2315 4 -0.9270468674203585 2315 5 -0.6572753961671935 2315 6 -1.0873684530512477 2315 7 -0.615939084047092 2315 8 -0.7039746676029068 2315 9 -0.8401467670734064 2315 10-11 -0.6375517579909342 2315 12-13 -0.970671853281452 2315 14-15 -0.9047859169515036 2315 16-17 -0.9573242840109728 2315 18-19 -0.7373462987185277 2315 20-21 -0.8482170807662115 2315 22-23 -0.9252028383103124 2315 24-25 -1.273821820742107 2315 26-27 -1.312899490796461 2315 28-29 -0.5377097446962793 2315 30-31 -0.293998411887344 2315 32-33 -0.5837729625946579 2315 34-35 -0.15031747202554868 2315 36-37 -0.9642076970086535 2315 38-39 -1.0799538644607694 2315 40-41 -1.2934442990734922 2315 42-43 -1.3623452517163557 2315 44-45 -0.837204318700941 2315 46-47 -1.7020506350837223 2315 48-49 -2.6508754295233032 2315 50-51 -2.049974582921134 2315 52-53 -1.994054326578702 2315 54-55 -1.8752371694358914 2315 56-57 -1.9173137660409338 2315 58-59 -1.783032298041352 2315 60-61 -1.8845789273977758 2315 62-63 -1.476610793058164 2315 64-65 -2.4777430843951294 2315 66-67 -2.0621340754844937 2315 68-69 -1.3521658947029103 2315 70-71 -1.3611644348643424 2315 72-73 -1.2071244263246435 2315 74-75 -1.3412509938527855 2315 76-77 -1.3008389800896332 2315 78-79 -1.4232333407944608 2315 80-81 -0.575171955710772 2315 82-83 -0.43960161498531036 2315 84-85 -1.1173570288148262 2315 86-87 -0.7842297808876175 2315 88-89 -0.9432353923119123 2315 90-91 -0.7529837947615867 2315 92-93 -0.5722163154430042 2315 94-95 -0.6033305097844526 2315 96-97 -0.6932839715597119 2315 98-99 -0.6730600150156967 2315 100-101 -0.25988653948346396 2316 1 -2.6263493180007735 2316 2 -1.6241631615061607 2316 3 -2.4176187622086758 2316 4 -1.0636285765657831 2316 5 -0.8357861407948803 2316 6 -0.6770236254650399 2316 7 -0.8283328871455424 2316 8 -0.5194169464634726 2316 9 -1.086273703605137 2316 10-11 -1.9390759958719954 2316 12-13 -1.6179482151005402 2316 14-15 -2.4078593802198682 2316 16-17 -1.8411823549754942 2316 18-19 -1.6688055690833394 2316 20-21 -1.5306008888621605 2316 22-23 -1.1121093850369448 2316 24-25 -1.145061201051952 2316 26-27 -1.7725196807015138 2316 28-29 -1.6072999495938305 2316 30-31 -1.2931738241811956 2316 32-33 -1.7626835073223006 2316 34-35 -1.1740306154538445 2316 36-37 -0.7990902557292898 2316 38-39 -0.9973451688086001 2316 40-41 -1.3522149137661543 2316 42-43 -0.6266630927958161 2316 44-45 -0.9958499958623577 2316 46-47 -0.9884074566729311 2316 48-49 -1.4924046649056137 2316 50-51 -2.3507991706272833 2316 52-53 -1.6065980547146346 2316 54-55 -1.3880807476467822 2316 56-57 -1.9048200129174973 2316 58-59 -1.7096439921942732 2316 60-61 -1.3219352492368515 2316 62-63 -0.9395543212940467 2316 64-65 -1.3507815651547475 2316 66-67 -1.2157072888777982 2316 68-69 -1.9062888332336456 2316 70-71 -1.4850025158238616 2316 72-73 -2.3102978396179985 2316 74-75 -2.0366532927033596 2316 76-77 -2.7597095448072757 2316 78-79 -3.7572663243027087 2316 80-81 -3.482918082647302 2316 82-83 -3.436278276654477 2316 84-85 -3.121754829914277 2316 86-87 -3.3296570672444403 2316 88-89 -3.8134502848656346 2316 90-91 -4.618076248534699 2316 92-93 -3.8226660905554475 2316 94-95 -4.285389480299198 2316 96-97 -5.004928149470754 2316 98-99 -4.345074008019193 2316 100-101 -4.731425769868274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 620.0 3 171.0 4 150.0 5 122.0 6 169.0 7 154.0 8 226.0 9 249.0 10 380.0 11 581.0 12 847.0 13 1104.0 14 1286.0 15 2017.0 16 2637.0 17 3849.0 18 3187.0 19 1567.0 20 1441.0 21 1436.0 22 1440.0 23 1486.0 24 1655.0 25 1846.0 26 2012.0 27 2248.0 28 2586.0 29 3147.0 30 3807.0 31 4863.0 32 6247.0 33 7989.0 34 11295.0 35 17163.0 36 24410.0 37 37080.0 38 37429.0 39 7291.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55722800952483 20.03375501608717 16.30132725539086 20.10768971899714 2 15.193616641598897 22.014428837280445 40.749483773931225 22.042470747189434 3 18.82325948963724 26.30484105335611 33.49733601855864 21.374563438448007 4 11.864510502883894 18.778207854596637 39.41832535226351 29.938956290255952 5 11.55449429316353 37.70797936661003 35.92832397075406 14.809202369472379 6 26.66932043195133 38.02743585032764 19.288790903709145 16.014452814011882 7 23.21907676613109 36.58097424126498 22.707982657485335 17.49196633511859 8 23.879759396348092 32.72587203518242 22.291040626100088 21.103327942369404 9 24.970030861835895 17.365776519499068 23.509067258397735 34.1551253602673 10-11 22.91403633618238 27.083285509142712 28.91080724683149 21.091870907843422 12-13 25.202963794268808 26.609108183592006 28.815630061341867 19.372297960797315 14-15 22.01787832030503 27.364286716229486 29.76931610961629 20.848518853849193 16-17 18.450518542318513 30.324589386393715 30.419959353014224 20.804932718273548 18-19 18.178966455584828 28.294062590058328 34.62141711318594 18.905553841170907 20-21 19.666541709062997 26.615204535195968 34.42734990424167 19.290903851499365 22-23 19.249440356540052 26.775433819292527 34.655570683351435 19.319555140815982 24-25 19.52714000244688 27.094378287998044 34.63638310019983 18.742098609355246 26-27 19.049099690339354 26.6578313560078 35.51310641876824 18.77996253488461 28-29 18.42731051849511 27.801920238972134 34.84976309236467 18.92100615016809 30-31 18.81754139140364 27.290800097871298 34.56360614957997 19.328052361145094 32-33 18.507455078373898 27.398496240601506 34.51076844654008 19.583280234484516 34-35 19.350678479309185 27.531681058652012 33.80519334876181 19.31244711327699 36-37 19.137954764417124 27.304471053610847 34.40840482635225 19.149169355619787 38-39 18.968052706026274 27.71475539357486 34.033361793228245 19.28383010717062 40-41 18.959237613643026 28.02774074989741 33.81623919642561 19.19678244003395 42-43 18.632300881572608 28.16852589539895 33.13326553966057 20.06590768336787 44-45 19.353062909687747 27.969885720693625 32.69601296728615 19.98103840233248 46-47 19.81115004778592 28.20516087926091 32.21866836572157 19.765020707231603 48-49 19.484683215250524 28.012640807380603 32.21188643661756 20.290789540751312 50-51 19.220182784792062 28.503417657642967 32.09234047108116 20.184059086483813 52-53 19.57910780233308 29.017960683765043 31.04494121425131 20.357990299650574 54-55 19.13138120533029 28.017913765153292 31.63405756466594 21.21664746485048 56-57 19.648155133917193 28.9209139198434 30.596280701038914 20.834650245200493 58-59 20.274747680701395 28.232745437863187 30.823988174125805 20.668518707309612 60-61 19.567705054654905 27.730387821729945 32.18739407346619 20.514513050148967 62-63 19.554772785779644 27.768842760979663 31.97948712720377 20.696897326036922 64-65 19.253255855442568 28.32173713586666 31.800341514386933 20.624665494303844 66-67 19.36070195832505 27.96157787668823 31.215171028413813 21.462549136572907 68-69 19.287446419510058 27.674434615865177 31.724494921806272 21.313624042818493 70-71 19.796852048310758 27.89905337032806 31.22628325444753 21.07781132691366 72-73 19.426094137076795 27.377384265630894 31.86862199386284 21.32789960342947 74-75 19.170742773247827 28.599102279476053 31.242560415163883 20.987594532112244 76-77 19.281193201950604 27.89525069527823 30.89915852853381 21.92439757423736 78-79 19.122835641302796 27.895753880039976 31.158097607733566 21.823312870923665 80-81 19.39582833852793 27.863107474609382 30.78985089549422 21.951213291368465 82-83 18.950035947195865 27.94652280989797 31.1771814339107 21.926259808995464 84-85 19.117204745081846 27.560524263232754 30.654411427347945 22.66785956433746 86-87 19.174187955739125 27.825455101728625 30.46453520983122 22.535821732701038 88-89 19.3788743851346 27.407250630484064 30.20688037250197 23.006994611879364 90-91 19.177918021291514 26.99381653598521 30.918340026773762 22.909925415949512 92-93 19.540766239561417 27.423216676228723 30.880346783961244 22.155670300248616 94-95 19.26508222963122 27.673864945605075 30.376533202826295 22.68451962193742 96-97 19.19835633478972 27.730477651965106 30.08330486828756 22.98786114495761 98-99 20.06649905911991 27.677173586002564 29.121131299944413 23.135196054933118 100-101 20.001479380496665 27.187442610239355 29.704940110596446 23.106137898667537 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1036.0 1 666.0 2 319.0 3 309.0 4 249.0 5 258.5 6 403.0 7 740.5 8 1138.0 9 1245.5 10 946.5 11 662.0 12 591.5 13 538.5 14 534.5 15 566.0 16 592.0 17 658.0 18 719.0 19 725.5 20 763.5 21 866.5 22 1003.5 23 1146.5 24 1289.5 25 1542.0 26 1946.5 27 2490.5 28 2786.0 29 3094.5 30 3413.0 31 3771.0 32 4243.0 33 4385.0 34 4969.0 35 5744.5 36 6246.0 37 6468.0 38 6079.5 39 5795.0 40 5748.5 41 5660.0 42 5640.0 43 5671.0 44 5866.5 45 5945.5 46 6010.0 47 6981.5 48 9595.5 49 9464.5 50 6794.5 51 5450.5 52 4700.5 53 4342.5 54 4052.5 55 3788.0 56 3441.5 57 2992.5 58 2665.5 59 2424.0 60 2072.5 61 1711.0 62 1377.0 63 992.0 64 660.5 65 508.0 66 381.0 67 251.5 68 166.0 69 109.0 70 79.5 71 59.5 72 38.0 73 28.0 74 26.0 75 26.5 76 16.5 77 8.5 78 5.5 79 2.5 80 4.5 81 3.0 82 2.0 83 1.5 84 0.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03669893114363044 2 0.028543613111712565 3 0.028543613111712565 4 0.05199015245347646 5 0.10092206064498371 6 0.12385889260975273 7 0.07186874015627628 8 0.09378615736705558 9 0.07951435081119929 10-11 0.0818080340076762 12-13 0.08078861925368645 14-15 0.07314300859876345 16-17 0.05734207991192257 18-19 0.07008476433679425 20-21 0.06345856843586098 22-23 0.04281541966756885 24-25 0.011723269670881947 26-27 0.004332512704456372 28-29 0.008919879097410176 30-31 0.00764561065492301 32-33 0.008155318031917876 34-35 0.008665025408912744 36-37 0.00917473278590761 38-39 0.004332512704456372 40-41 0.008919879097410176 42-43 0.003822805327461505 44-45 0.002548536884974336 46-47 0.0017839758194820353 48-49 5.097073769948673E-4 50-51 0.002038829507979469 52-53 0.006371342212435841 54-55 0.014781513932851148 56-57 0.012742684424871682 58-59 0.00560678114694354 60-61 0.00280339057347177 62-63 0.010958708605389646 64-65 0.0030582442619692036 66-67 0.02752419835772283 68-69 0.05479354302694823 70-71 0.06473283687834813 72-73 0.004587366392953805 74-75 0.014016952867358849 76-77 0.023701393030261326 78-79 0.03007273524269717 80-81 0.07849493605720956 82-83 0.0356795163896407 84-85 0.01529122130984602 86-87 0.04128629753658425 88-89 0.0568323725349277 90-91 0.052754713518968765 92-93 0.052754713518968765 94-95 0.01682034344083062 96-97 0.02242712458777416 98-99 0.04995132294549699 100-101 0.08308230245016336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 196191.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.4389350829974 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61684173171687 47.91779222287795 2 12.86525722872997 15.293943784890876 3 3.2101446780783465 5.724227433819852 4 1.1485871862955592 2.7308319681353757 5 0.5627255492436047 1.672390369551734 6 0.3223208648369164 1.1495045376562232 7 0.20724056417786318 0.8622710908512237 8 0.13980689106159505 0.664797817757267 9 0.10212732786098666 0.546330564983626 >10 0.6891092045099309 7.853695271469313 >50 0.07436429205567567 3.080538950987419 >100 0.05354229028008648 6.352027704944022 >500 0.0029745716822270267 1.3714203441644774 >1k 0.004957619470378378 4.780227937910647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2481 1.2645840023242656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.9878128966160526 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1347 0.6865758368120861 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1270 0.6473283687834814 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1075 0.5479354302694823 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 901 0.4592463466723754 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 807 0.4113338532348579 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 619 0.31550886635982284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.2431304188265517 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 384 0.19572763276602903 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 361 0.18400436309514706 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 360 0.1834946557181522 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 355 0.18094611883317788 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 319 0.16259665326136266 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 307 0.15648016473742427 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 295 0.15036367621348584 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 281 0.14322777293555772 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 281 0.14322777293555772 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 268 0.13660157703462442 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 249 0.12691713687172196 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 248 0.12640742949472708 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 247 0.1258977221177322 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 245 0.12487830736374247 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 243 0.12385889260975273 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 233 0.11876181883980408 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 227 0.11570357457783487 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 214 0.10907737867690159 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 214 0.10907737867690159 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 213 0.10856767129990672 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.097073769948673E-4 0.0 18-19 0.0 0.0 0.0 5.097073769948673E-4 0.0 20-21 0.0 0.0 0.0 5.097073769948673E-4 0.0 22-23 0.0 0.0 0.0 7.645610654923009E-4 0.0 24-25 0.0 0.0 0.0 0.0025485368849743366 0.0 26-27 0.0 0.0 0.0 0.005861634835440973 0.0 28-29 0.0 0.0 0.0 0.018349465571815222 0.0 30-31 0.0 0.0 0.0 0.03567951638964071 0.0 32-33 0.0 0.0 0.0 0.051735298764979024 0.0 34-35 0.0 0.0 0.0 0.06473283687834815 0.0 36-37 0.0 0.0 0.0 0.0818080340076762 0.0 38-39 0.0 0.0 0.0 0.09251188892456841 0.0 40-41 0.0 0.0 0.0 0.11239047662736823 0.0 42-43 0.0 0.0 0.0 0.15698987211441912 0.0 44-45 0.0 0.0 0.0 0.24287556513805425 0.0 46-47 0.0 0.0 0.0 0.3259578675882176 0.0 48-49 0.0 0.0 0.0 0.4671468110157958 0.0 50-51 0.0 0.0 0.0 0.6098648765743586 0.0 52-53 0.0 0.0 0.0 0.7607382601648394 0.0 54-55 0.0 0.0 0.0 0.9758347732566733 0.0 56-57 0.0 0.0 0.0 1.2235525584761788 0.0 58-59 0.0 0.0 0.0 1.5008333715613866 0.0 60-61 0.0 0.0 0.0 1.8041092608733327 0.0 62-63 0.0 0.0 0.0 2.2215596026321287 0.0 64-65 0.0 0.0 0.0 2.594665402592372 0.0 66-67 0.0 0.0 0.0 3.0281715267265064 0.0 68-69 0.0 0.0 0.0 3.601337472157234 0.0 70-71 0.0 0.0 0.0 4.0779138696474355 0.0 72-73 0.0 0.0 0.0 4.639101691718784 0.0 74-75 0.0 0.0 0.0 5.318286771564445 0.0 76-77 0.0 0.0 0.0 5.924838550188337 0.0 78-79 0.0 0.0 0.0 6.484242396440203 0.0 80-81 0.0 0.0 0.0 7.2194952877553 0.0 82-83 0.0 0.0 0.0 8.07045175364823 0.0 84-85 0.0 0.0 0.0 9.01978174330117 0.0 86-87 0.0 0.0 0.0 9.974718514101056 0.0 88-89 0.0 0.0 0.0 10.982919705796903 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 55 4.9445916E-5 43.187153 9 TGCAATT 45 9.5755997E-4 42.248985 2 TAGGACC 70 2.0395378E-4 33.950077 4 CCTAGGA 60 0.003930816 31.68674 2 CCCTAGG 65 0.005821629 29.241835 1 TATAGGT 150 3.286732E-8 28.518066 3 TACTACA 70 0.008349044 27.16006 2 TTATAGG 175 1.4640682E-7 24.444056 2 TTAGGCA 100 0.0016427013 23.765055 4 ATAGGTG 180 1.9221079E-7 23.765055 4 GTTATAG 245 2.910383E-10 23.274115 1 GAGTACT 1735 0.0 23.136864 12-13 TAGGTGG 185 2.5040208E-7 23.122757 5 AGTACTT 1750 0.0 23.07428 12-13 GTATCAA 3560 0.0 21.623352 1 GTACTTT 1885 0.0 21.424475 14-15 TTTAGGC 135 3.764319E-4 21.124493 3 TGGATTA 180 5.1272764E-6 21.113718 9 GGTGGAT 205 6.7619294E-7 20.856234 7 CTGTACT 115 0.003698822 20.665264 4 >>END_MODULE