FastQCFastQC Report
Thu 26 May 2016
SRR936795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099934
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70320.33486766726954276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51690.24615059330436098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.150385678788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.050.1978841
GTATCAA72450.042.1157151
TCAACGC90850.033.1666454
TATCAAC92050.032.9942
ATCAACG91400.032.9654923
CAACGCA93650.032.1734735
AACGCAG97800.030.9054266
ACGCAGA110300.027.1862667
CGCAGAG112150.026.567688
GTACATG84700.025.3070011
TACATGG87200.024.1459982
GCAGAGT123000.023.6832189
ACATGGG90300.022.8411773
CGCGGAA2500.021.85443782-83
ATTCGCG2900.021.2948980-81
GAGTACT63650.021.19833412-13
GTGGTAT21800.020.7116471
CATGGGG54050.020.5758294
GTATCGA1404.8344728E-420.3690511
CTCGCGC3050.020.24325460-61
Produced by FastQC (version 0.11.5)