##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936795_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099934 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.600745547241008 33.0 31.0 34.0 30.0 34.0 2 31.711594745358664 33.0 31.0 34.0 30.0 34.0 3 31.72175935053197 33.0 31.0 34.0 30.0 34.0 4 35.285748028271364 37.0 35.0 37.0 33.0 37.0 5 35.30127089708534 37.0 35.0 37.0 33.0 37.0 6 35.34396700086764 37.0 35.0 37.0 33.0 37.0 7 35.27396432459306 37.0 35.0 37.0 33.0 37.0 8 35.23813319847195 37.0 35.0 37.0 32.0 37.0 9 36.834203836882494 39.0 37.0 39.0 33.0 39.0 10-11 36.69816241843791 39.0 37.0 39.0 32.0 39.0 12-13 36.58683201472046 39.0 37.0 39.0 32.0 39.0 14-15 37.7334137644326 40.0 38.0 41.0 32.0 41.0 16-17 37.64933397906792 40.0 37.0 41.0 32.0 41.0 18-19 37.574882115342675 40.0 37.0 41.0 32.0 41.0 20-21 37.503534634898045 40.0 37.0 41.0 32.0 41.0 22-23 37.35470662411295 40.0 37.0 41.0 32.0 41.0 24-25 37.18075710950916 40.0 37.0 41.0 31.0 41.0 26-27 37.13441493875522 39.5 37.0 41.0 31.0 41.0 28-29 37.20958182495259 40.0 37.0 41.0 31.0 41.0 30-31 37.07976941180056 40.0 37.0 41.0 31.0 41.0 32-33 36.90908380930067 39.5 36.0 41.0 30.0 41.0 34-35 36.72337249646894 39.0 36.0 41.0 30.0 41.0 36-37 36.58186423954277 39.0 36.0 41.0 30.0 41.0 38-39 36.4143813567474 39.0 35.0 41.0 30.0 41.0 40-41 36.205676464117445 39.0 35.0 41.0 30.0 41.0 42-43 35.999413791100096 39.0 35.0 40.5 29.0 41.0 44-45 35.75402631701758 39.0 35.0 40.0 28.0 41.0 46-47 35.43705516459089 38.5 35.0 40.0 26.5 41.0 48-49 35.10400684021498 38.0 34.5 40.0 26.0 41.0 50-51 34.45796010731766 37.5 33.5 39.5 24.5 40.5 52-53 34.76823343019352 38.0 34.0 40.0 26.0 40.5 54-55 35.058993996954186 39.0 35.0 40.0 26.0 41.0 56-57 34.92305424837161 38.0 34.5 40.0 26.0 41.0 58-59 34.65419341750741 38.0 34.0 40.0 25.5 41.0 60-61 34.34297030287618 38.0 34.0 40.0 24.5 41.0 62-63 34.012009425058125 37.0 34.0 40.0 23.5 41.0 64-65 33.672075646186975 37.0 33.0 39.5 23.0 41.0 66-67 33.28266578854383 36.0 33.0 39.0 22.0 41.0 68-69 32.83524958403454 35.5 32.5 39.0 21.0 41.0 70-71 32.47008310737385 35.0 32.0 38.5 20.5 40.0 72-73 32.089590672849724 35.0 32.0 37.5 20.0 40.0 74-75 31.659008806943454 35.0 31.5 37.0 18.5 39.0 76-77 31.223918466008932 35.0 31.0 36.5 17.0 39.0 78-79 30.818195476619742 35.0 31.0 36.0 12.5 38.5 80-81 30.426538643595464 34.5 30.5 35.5 9.0 37.0 82-83 30.036354237799856 34.0 30.0 35.0 7.0 37.0 84-85 29.702832803316674 34.0 30.0 35.0 4.5 36.5 86-87 29.3856028332319 34.0 29.5 35.0 2.0 36.0 88-89 29.02619177555104 34.0 29.0 35.0 2.0 36.0 90-91 28.70275446752136 34.0 29.0 35.0 2.0 35.0 92-93 28.351256039475526 34.0 29.0 35.0 2.0 35.0 94-95 27.948400759261958 34.0 28.5 35.0 2.0 35.0 96-97 27.497679927083425 34.0 27.0 35.0 2.0 35.0 98-99 26.896147212245715 34.0 26.0 35.0 2.0 35.0 100-101 24.756208290355794 31.0 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4651229609658394 1101 2 0.35597856486043966 1101 3 0.5538002711971011 1101 4 0.4442495679893881 1101 5 0.34668638779744043 1101 6 0.25203196509578163 1101 7 0.3628238573477631 1101 8 0.31687562016202975 1101 9 0.44412873732765235 1101 10-11 0.4659845400025091 1101 12-13 0.5682080192616894 1101 14-15 0.7354676442845474 1101 16-17 0.7863526469585054 1101 18-19 0.8581420561203359 1101 20-21 0.8130736529969909 1101 22-23 0.6660658549594132 1101 24-25 0.7916962497699203 1101 26-27 0.8429917321810336 1101 28-29 0.9086863438398822 1101 30-31 0.7672088132449773 1101 32-33 0.8180592073252058 1101 34-35 0.7654562920464798 1101 36-37 0.8155876916186813 1101 38-39 0.7915742672395538 1101 40-41 0.9527540714527376 1101 42-43 0.9851211341288035 1101 44-45 0.9260006102907639 1101 46-47 0.9926417155317537 1101 48-49 0.913888083763652 1101 50-51 0.9741109734478641 1101 52-53 1.0452336462756477 1101 54-55 1.11216163489253 1101 56-57 0.9554886067082222 1101 58-59 0.9896798233549404 1101 60-61 1.0840337123352626 1101 62-63 1.1859068535882713 1101 64-65 0.9626525256006886 1101 66-67 0.9558938933150429 1101 68-69 0.8050221170824656 1101 70-71 0.7931114001117834 1101 72-73 0.9276059068059581 1101 74-75 0.9203302185335502 1101 76-77 0.8909728407531254 1101 78-79 0.9242521223507509 1101 80-81 0.9001833441526266 1101 82-83 0.9346522794667216 1101 84-85 1.2092616574590274 1101 86-87 1.2143252316942146 1101 88-89 1.220516640865494 1101 90-91 1.1072543656590454 1101 92-93 1.014747363154445 1101 94-95 1.18734340499741 1101 96-97 1.0944290462869546 1101 98-99 1.3051650104008168 1101 100-101 1.3963170307335986 1102 1 0.49827315828030194 1102 2 0.4656839320978712 1102 3 0.3711793059371793 1102 4 0.3766344450893939 1102 5 0.33416464006638336 1102 6 0.4640283476201503 1102 7 0.36586788206822973 1102 8 0.34422344128532956 1102 9 0.42171890722168826 1102 10-11 0.5783438275134287 1102 12-13 0.4635545440702771 1102 14-15 0.6872120135490576 1102 16-17 0.6047612628216257 1102 18-19 0.6301529695750148 1102 20-21 0.542719960052068 1102 22-23 0.5892734131475805 1102 24-25 0.6767151341922144 1102 26-27 0.6143394943809355 1102 28-29 0.694456659390454 1102 30-31 0.6655370197167727 1102 32-33 0.7982638398615478 1102 34-35 0.5928154769675587 1102 36-37 0.5430967103179185 1102 38-39 0.5092217717022152 1102 40-41 0.6682645506104237 1102 42-43 0.47747161469644794 1102 44-45 0.5064192084084809 1102 46-47 0.44421431257516275 1102 48-49 0.41390071020563113 1102 50-51 0.3577225356912237 1102 52-53 0.5803969719331619 1102 54-55 0.6989144980697759 1102 56-57 0.7446288670128496 1102 58-59 0.5738140150758895 1102 60-61 0.6095459644558474 1102 62-63 0.6612197920611962 1102 64-65 0.7644760468733409 1102 66-67 0.5651036727048009 1102 68-69 0.6079254621952188 1102 70-71 0.6721477184938323 1102 72-73 0.6638976681051858 1102 74-75 0.688750487550422 1102 76-77 0.6270815186436494 1102 78-79 0.7304678040760315 1102 80-81 0.8777080143789 1102 82-83 0.8126990004586538 1102 84-85 0.7278754003941401 1102 86-87 1.0204833052779598 1102 88-89 0.9218528866981153 1102 90-91 0.9709598160205566 1102 92-93 0.89492032436549 1102 94-95 0.9010646121063068 1102 96-97 0.827790157792645 1102 98-99 0.9920566066093066 1102 100-101 1.3153594413739 1103 1 0.2037111218425629 1103 2 0.2951259595124931 1103 3 0.2083999786044224 1103 4 0.3063967483575638 1103 5 0.245794132072767 1103 6 0.16938370274245074 1103 7 0.19584764041353964 1103 8 0.2299668284279761 1103 9 0.29640069657913415 1103 10-11 0.2676833204355731 1103 12-13 0.1758293476855286 1103 14-15 0.30145284629731606 1103 16-17 0.3920362518315983 1103 18-19 0.4897305213954226 1103 20-21 0.43323670085075605 1103 22-23 0.3175883096223089 1103 24-25 0.5961998437838716 1103 26-27 0.5535449426058889 1103 28-29 0.4850538356304668 1103 30-31 0.5577504240485212 1103 32-33 0.6435498728783671 1103 34-35 0.5137055647998139 1103 36-37 0.5700174039735728 1103 38-39 0.5916886218320485 1103 40-41 0.8485942048501229 1103 42-43 0.7071093672019018 1103 44-45 0.7533225560163359 1103 46-47 0.6599321980267447 1103 48-49 0.6258125713116982 1103 50-51 0.6230644665108755 1103 52-53 0.7233645878725525 1103 54-55 0.7357102632748607 1103 56-57 0.7863652722764058 1103 58-59 0.766195619849178 1103 60-61 0.6074103896916725 1103 62-63 0.5220317278252864 1103 64-65 0.5461071030938172 1103 66-67 0.6759829110653257 1103 68-69 0.7036969275844385 1103 70-71 0.5634245776623032 1103 72-73 0.4502245571178989 1103 74-75 0.5559229282711087 1103 76-77 0.4686046423671222 1103 78-79 0.47085319055677033 1103 80-81 0.6095309239585305 1103 82-83 0.47454523183230535 1103 84-85 0.710147870622766 1103 86-87 0.6352799772788238 1103 88-89 0.7494908078696554 1103 90-91 0.7614105735433974 1103 92-93 0.6884208682187598 1103 94-95 0.6230787610867488 1103 96-97 0.7724325003265591 1103 98-99 0.6771641847005831 1103 100-101 0.8872461163910899 1104 1 0.4200243970263422 1104 2 0.4483019098046448 1104 3 0.37582517314160313 1104 4 0.3731902030897558 1104 5 0.39606307924011475 1104 6 0.4077955042818857 1104 7 0.41452199930075295 1104 8 0.29149219633193724 1104 9 0.35718779209742024 1104 10-11 0.40527218581465974 1104 12-13 0.476254695073024 1104 14-15 0.5611462523985509 1104 16-17 0.5015239683777111 1104 18-19 0.5071865973913958 1104 20-21 0.5302059939509434 1104 22-23 0.639365102957818 1104 24-25 0.5415915311820143 1104 26-27 0.5678644730867077 1104 28-29 0.5502053505123783 1104 30-31 0.557962528334798 1104 32-33 0.491481331164735 1104 34-35 0.5889092569802727 1104 36-37 0.5479050872805189 1104 38-39 0.5073563490794584 1104 40-41 0.5220205093760057 1104 42-43 0.5543038876513791 1104 44-45 0.3679239076117611 1104 46-47 0.27770434855602133 1104 48-49 0.13715507934168158 1104 50-51 0.15450287699566445 1104 52-53 0.4169541450817178 1104 54-55 0.30061800057942634 1104 56-57 0.4829484893112266 1104 58-59 0.41653431943281305 1104 60-61 0.46967187690928114 1104 62-63 0.4560115106568361 1104 64-65 0.428082544905088 1104 66-67 0.18481949236416995 1104 68-69 0.3397354921295346 1104 70-71 0.28519286547359 1104 72-73 0.2781053986380968 1104 74-75 0.25757636303489306 1104 76-77 0.26898568273508516 1104 78-79 0.410429359216689 1104 80-81 0.4426817206752567 1104 82-83 0.483347915592109 1104 84-85 0.6587239693523266 1104 86-87 0.3780907185103466 1104 88-89 0.47828558398308374 1104 90-91 0.37991352298517356 1104 92-93 0.24472521438149286 1104 94-95 0.38993148757880647 1104 96-97 0.27050265381924277 1104 98-99 0.19522623481306667 1104 100-101 0.4711246425581237 1105 1 0.31785691613087863 1105 2 0.3538935933929004 1105 3 0.3593645724383414 1105 4 0.33236506916561837 1105 5 0.38171057163659583 1105 6 0.258442418154587 1105 7 0.35215896457525275 1105 8 0.28112702445005766 1105 9 0.41913114575494603 1105 10-11 0.44857309074281915 1105 12-13 0.31328500551454397 1105 14-15 0.41208178839945475 1105 16-17 0.5562084480929101 1105 18-19 0.5090089015757187 1105 20-21 0.5348394849754143 1105 22-23 0.6122667021876254 1105 24-25 0.6778774743704332 1105 26-27 0.6036392681311824 1105 28-29 0.49116192723093377 1105 30-31 0.34317895197577286 1105 32-33 0.28904882606175164 1105 34-35 0.2850751523733024 1105 36-37 0.3043219029650359 1105 38-39 0.40774899581383295 1105 40-41 0.48378968442304426 1105 42-43 0.44944869315680336 1105 44-45 0.4029709325464026 1105 46-47 0.5528787693604968 1105 48-49 0.5227325822429805 1105 50-51 0.4572293781917409 1105 52-53 0.5298662655842961 1105 54-55 0.5573409860326208 1105 56-57 0.43448254608259873 1105 58-59 0.3158869028933182 1105 60-61 0.22339311911405701 1105 62-63 0.1762680315702383 1105 64-65 0.34629427217601005 1105 66-67 0.516538899743793 1105 68-69 0.5328205884980619 1105 70-71 0.4710341679033334 1105 72-73 0.5152400992616393 1105 74-75 0.5021901056523177 1105 76-77 0.3527883697540908 1105 78-79 0.4346952783538711 1105 80-81 0.3811567685702606 1105 82-83 0.5051260911164057 1105 84-85 0.4406864938070676 1105 86-87 0.5453349036152879 1105 88-89 0.6356086804616545 1105 90-91 0.6538753287178061 1105 92-93 0.47905366343877276 1105 94-95 0.629677313662313 1105 96-97 0.723669829276087 1105 98-99 0.6404223945850624 1105 100-101 0.7798984993521927 1106 1 0.5636698462561789 1106 2 0.5035124839290255 1106 3 0.6181557612474329 1106 4 0.48556465586026576 1106 5 0.4937781466337654 1106 6 0.478838352701743 1106 7 0.6063152290821208 1106 8 0.4268665779005758 1106 9 0.5467562872136185 1106 10-11 0.6958629671148984 1106 12-13 0.6932536011534438 1106 14-15 0.8761380210313305 1106 16-17 0.9131608345462467 1106 18-19 0.8587790379108711 1106 20-21 0.9286808715592514 1106 22-23 0.9288941134029187 1106 24-25 0.9948579707219238 1106 26-27 1.0147146535726534 1106 28-29 0.9660577986698726 1106 30-31 0.9340184196419088 1106 32-33 0.9421655163672611 1106 34-35 0.9450509573239785 1106 36-37 0.8390600894273703 1106 38-39 0.9162616527554093 1106 40-41 0.8230161488011234 1106 42-43 0.9775035053189285 1106 44-45 1.0771618103068548 1106 46-47 0.8808596700324429 1106 48-49 0.9487353076623322 1106 50-51 0.7623686334291833 1106 52-53 0.9111460146916883 1106 54-55 1.0370291244685248 1106 56-57 1.0786887837417254 1106 58-59 1.292731427774008 1106 60-61 1.2904629296396521 1106 62-63 1.151521801484094 1106 64-65 1.0240599631254312 1106 66-67 1.116746301161264 1106 68-69 1.0565247842319465 1106 70-71 1.0385101172541766 1106 72-73 0.9965357858683603 1106 74-75 0.8882142022956003 1106 76-77 1.0461590118106905 1106 78-79 1.0852103055973679 1106 80-81 1.2680836336320525 1106 82-83 1.298355434940678 1106 84-85 1.2208254816368047 1106 86-87 1.222930309336956 1106 88-89 1.1748531146376457 1106 90-91 1.1579838876897632 1106 92-93 1.2517774355560647 1106 94-95 1.20172197716715 1106 96-97 1.2549120343414302 1106 98-99 1.5085152000341928 1106 100-101 1.7563377411122936 1107 1 0.1473993349049998 1107 2 0.34288365376832175 1107 3 0.3141200631889909 1107 4 0.2581927536139901 1107 5 0.048642662646123824 1107 6 0.17234841312531302 1107 7 0.28789180290942085 1107 8 0.2581333480988448 1107 9 0.22921830490441408 1107 10-11 0.3440786293215652 1107 12-13 0.31985511976765935 1107 14-15 0.27046152790442335 1107 16-17 0.43989312397359015 1107 18-19 0.5082944964451102 1107 20-21 0.45265962723597397 1107 22-23 0.44908933150874475 1107 24-25 0.34297744800035446 1107 26-27 0.28260335483876986 1107 28-29 0.23435870491047694 1107 30-31 0.3108824971142923 1107 32-33 0.4187525676832138 1107 34-35 0.4839805354892164 1107 36-37 0.44535788519884534 1107 38-39 0.4424808051977891 1107 40-41 0.3734205549976508 1107 42-43 0.5179186941736802 1107 44-45 0.5779441746702858 1107 46-47 0.4955005758661528 1107 48-49 0.5708148913658277 1107 50-51 0.2759135283943408 1107 52-53 0.4159095986935739 1107 54-55 0.6417002533924929 1107 56-57 0.6165231422187745 1107 58-59 0.5162779729153684 1107 60-61 0.4918026240631903 1107 62-63 0.4878029598625844 1107 64-65 0.6365573352763363 1107 66-67 0.5658104478054184 1107 68-69 0.6343591879193085 1107 70-71 0.5366465483977407 1107 72-73 0.3769781461176578 1107 74-75 0.5946129300775596 1107 76-77 0.6413796042013438 1107 78-79 0.7404098299849373 1107 80-81 0.5358293573021342 1107 82-83 0.6158347568764917 1107 84-85 0.6309242209473958 1107 86-87 0.7464938083434625 1107 88-89 0.5799161038979364 1107 90-91 0.588846364717277 1107 92-93 0.6812889155832842 1107 94-95 0.6637895123238167 1107 96-97 0.7227089586842439 1107 98-99 0.669761263911834 1107 100-101 0.4834539790722232 1108 1 0.013416407035748534 1108 2 0.17755166331858518 1108 3 -0.002433283018202559 1108 4 0.0962191446377787 1108 5 0.009666013422091169 1108 6 0.010324191976309294 1108 7 -0.10058984363158174 1108 8 -0.1998329492748283 1108 9 0.021704878999656785 1108 10-11 -0.010057381806085175 1108 12-13 -0.09798451820093135 1108 14-15 0.209476302359775 1108 16-17 0.1205236695728189 1108 18-19 0.15559936893173898 1108 20-21 0.24313236447125064 1108 22-23 0.09282213753749602 1108 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2316 94-95 -2.231672592926053 2316 96-97 -2.3551726084688767 2316 98-99 -2.4776246325605875 2316 100-101 -2.2612563841175017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8223.0 3 2680.0 4 2685.0 5 2786.0 6 3003.0 7 3387.0 8 4008.0 9 4584.0 10 5560.0 11 6607.0 12 7703.0 13 9002.0 14 9643.0 15 11438.0 16 13920.0 17 17796.0 18 16214.0 19 11992.0 20 11834.0 21 12769.0 22 13771.0 23 15350.0 24 17539.0 25 20228.0 26 23686.0 27 27757.0 28 32928.0 29 39539.0 30 48676.0 31 60026.0 32 77426.0 33 100738.0 34 137871.0 35 196458.0 36 297896.0 37 424495.0 38 342925.0 39 56763.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.326859714216695 18.01757544945188 14.329952070854516 23.32561276547691 2 15.660506234869223 19.434078549676137 40.27385663876177 24.63155857669287 3 19.47903358695616 23.837432327403608 29.16355932243773 27.519974763202498 4 13.302391403649139 16.103329007057773 36.17063580992742 34.42364377936567 5 14.700471879121913 36.356191274311975 33.512760852766945 15.430575993799167 6 32.79369923204266 34.8893633309071 17.350087206129167 14.966850230921066 7 29.218992755270985 31.95990013648522 20.29820805343852 18.522899054805276 8 26.803872118468636 34.39825012047343 19.353204259540043 19.444673501517894 9 26.63094287450985 16.75582517907584 19.804822235200174 36.80840971121414 10-11 25.435524206965525 26.077798467084456 26.868687735436207 21.617989590513808 12-13 26.308376536375157 23.23779491229667 27.491648153347008 22.962180397981165 14-15 23.365925063013112 24.611816341083028 25.520311004511015 26.50194759139285 16-17 23.016266307603235 27.84276625671367 25.950866183323047 23.190101252360048 18-19 22.925675633811075 26.49583607584047 28.190550587400942 22.387937702947514 20-21 23.650666875075053 25.559700425648717 28.308784985446362 22.480847713829867 22-23 23.47000922133345 25.417259381574965 28.211013655674932 22.901717741416654 24-25 23.191479223399973 25.633018604950358 28.234542929094385 22.94095924255528 26-27 23.092818576675096 25.81103333136396 28.115298045934733 22.980850046026212 28-29 22.896303668312026 25.848875389469704 28.287181505689823 22.96763943652844 30-31 23.086248498912166 25.683568183155465 28.18557578763487 23.044607530297498 32-33 22.943038502383857 25.769697495222516 28.17387509415606 23.113388908237564 34-35 23.273285291088172 25.8162750966419 28.01393148882365 22.896508123446274 36-37 23.26836052405142 25.710903635180323 27.944382665405037 23.076353175363217 38-39 23.11834501790385 25.783113185050578 28.04592323274097 23.052618564304602 40-41 23.274661520225397 25.730467051458454 27.966000131043643 23.028871297272506 42-43 23.187730061349694 25.8159509202454 27.775996187980223 23.220322830424685 44-45 23.223159435397882 25.86698041921126 27.69070281776974 23.21915732762112 46-47 23.314004242157903 26.036011895191784 27.41610662322087 23.23387723942944 48-49 23.418789696260408 26.14994243647705 27.16464692109593 23.266620946166615 50-51 23.335789656197363 26.2988104855612 27.08800939722123 23.277390461020204 52-53 23.484638253810093 26.381147003077327 26.891351206241453 23.24286353687112 54-55 23.315299769194837 26.20142437444511 26.986266747309912 23.49700910905014 56-57 23.20885246682707 26.51635419396336 26.963122662410367 23.3116706767992 58-59 23.392330587285972 26.250938968086878 27.025389601772705 23.331340842854445 60-61 23.336385909106934 26.13759270888624 27.26079606475018 23.265225317256647 62-63 23.25362593000971 26.215600242824618 27.253513475908616 23.277260351257056 64-65 23.258270769286472 26.197816249168078 27.192171311865515 23.35174166967994 66-67 23.174312735347268 26.422650041515226 26.963884729424347 23.439152493713152 68-69 23.075097842462913 26.322409854858677 27.21078225370715 23.39171004897126 70-71 23.17806774532817 26.142759427491384 27.092200980093285 23.586971847087156 72-73 23.14815586600388 26.360217165112694 26.891793960348338 23.599833008535093 74-75 23.065721594238106 26.592928152583468 26.746049892583756 23.59530036059467 76-77 23.293856513746693 26.452557902101265 26.70632214313942 23.547263441012618 78-79 23.261025621598776 26.441716753617474 26.719092080650892 23.578165544132865 80-81 23.165164713078905 26.5760746320706 26.73171691493842 23.527043739912074 82-83 23.22806562788723 26.51591642560256 26.746894835375173 23.50912311113504 84-85 23.26738606113066 26.516669323236936 26.499705394696054 23.716239220936348 86-87 23.244419083108276 26.39494033401515 26.81813579089446 23.54250479198211 88-89 23.256424695634685 26.23800966161931 26.693160535352177 23.812405107393825 90-91 23.216866522602025 26.3524882123516 26.760904319318403 23.669740945727973 92-93 23.300280788571733 26.297288951074542 26.693452460378886 23.708977799974836 94-95 23.210078010020055 26.279673166230737 26.692814575596515 23.817434248152693 96-97 23.193335589439517 26.220656717556164 26.71512528538949 23.870882407614836 98-99 22.998264745195986 26.280325538473715 26.694340790312133 24.027068926018167 100-101 23.167940332464198 26.22447602824139 26.607124042128472 24.000459597165943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 289.0 1 242.0 2 217.0 3 246.5 4 239.5 5 249.5 6 397.0 7 817.0 8 1434.5 9 1815.0 10 1732.0 11 1652.0 12 1773.0 13 1911.5 14 2033.0 15 2160.0 16 2318.0 17 2444.0 18 2576.5 19 2745.5 20 3023.5 21 3497.5 22 4105.5 23 4953.5 24 6062.5 25 7497.0 26 9415.5 27 11585.0 28 13605.5 29 15945.0 30 18745.0 31 21681.5 32 24824.0 33 27876.5 34 31285.5 35 34722.0 36 38476.5 37 42226.0 38 46038.5 39 49172.5 40 51894.5 41 55801.5 42 59379.5 43 63423.5 44 67899.0 45 72006.5 46 75995.5 47 79714.5 48 84009.0 49 85872.0 50 84990.5 51 84365.0 52 83811.5 53 83783.5 54 83127.0 55 79508.5 56 74307.5 57 67725.5 58 61507.0 59 55322.5 60 47517.0 61 39391.0 62 31306.5 63 24270.0 64 18009.0 65 12981.0 66 9269.0 67 6867.5 68 4830.5 69 3190.5 70 2225.0 71 1403.5 72 876.5 73 534.5 74 308.5 75 182.5 76 115.5 77 69.0 78 42.5 79 29.5 80 30.0 81 24.0 82 18.0 83 20.0 84 16.0 85 12.5 86 10.5 87 6.5 88 7.0 89 6.5 90 2.5 91 5.5 92 8.5 93 5.5 94 3.0 95 3.5 96 2.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05766847910458139 2 0.0940505749228309 3 0.06719258795752629 4 0.1272420942753439 5 0.10243179071342241 6 0.09671732540165548 7 0.10814625602518935 8 0.09338388730312476 9 0.11233686392048511 10-11 0.1062890547988651 12-13 0.09024093138165294 14-15 0.09324102567033059 16-17 0.10736051704482141 18-19 0.08538363586665106 20-21 0.07095461095443951 22-23 0.07328801762341101 24-25 0.06185908699987714 26-27 0.0538112150191387 28-29 0.06702591605259975 30-31 0.04950155576318113 32-33 0.04881105787134262 34-35 0.045144275962958835 36-37 0.05028729474354909 38-39 0.05159685971082901 40-41 0.06650209006568777 42-43 0.06233529244252438 44-45 0.04952536603531349 46-47 0.036501147178911335 48-49 0.0451680862350912 50-51 0.028096121116187457 52-53 0.041548924870972136 54-55 0.04654908201876821 56-57 0.05162066998296137 58-59 0.05812087427509627 60-61 0.05523983134708043 62-63 0.06190670754414187 64-65 0.042144181674281196 66-67 0.06221624108186258 68-69 0.054858866992962635 70-71 0.025810334991480686 72-73 0.02026254158464028 74-75 0.029715219621188095 76-77 0.044691880792443954 78-79 0.06350199577701013 80-81 0.04238228439560481 82-83 0.05966854196369981 84-85 0.06493061210495187 86-87 0.06554967918039328 88-89 0.08755037063069601 90-91 0.06376390877046612 92-93 0.07419280796444079 94-95 0.0829549881091501 96-97 0.09162192716532996 98-99 0.1213133365143857 100-101 0.11643223072725142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2099934.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.51924622067194 #Duplication Level Percentage of deduplicated Percentage of total 1 77.44283241356604 46.09339010449271 2 12.684625425549081 15.099586878405027 3 4.166375805213816 7.439386421951142 4 1.8582397126670862 4.42404107981052 5 1.0245641672906192 3.0490643470924037 6 0.6284491476238411 2.2442891732756887 7 0.4242684317488259 1.76764960770417 8 0.31276714673398254 1.489253185295755 9 0.23040930632053672 1.2342409410983624 >10 1.1523429402209837 12.131924814359076 >50 0.054567908611837725 2.195376421712835 >100 0.01983558476236705 1.8818445394799872 >500 4.011164949828219E-4 0.15179185477957538 >1k 1.6044659799311885E-4 0.21558358739532787 >5k 1.6044659799311885E-4 0.5825770431473744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7032 0.33486766726954276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5169 0.24615059330436098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3158 0.150385678788 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.762054426472451E-5 0.0 5 0.0 0.0 0.0 4.762054426472451E-5 0.0 6 0.0 0.0 0.0 4.762054426472451E-5 4.762054426472451E-5 7 4.762054426472451E-5 0.0 0.0 4.762054426472451E-5 4.762054426472451E-5 8 4.762054426472451E-5 4.762054426472451E-5 0.0 4.762054426472451E-5 4.762054426472451E-5 9 4.762054426472451E-5 4.762054426472451E-5 0.0 9.524108852944902E-5 4.762054426472451E-5 10-11 9.524108852944902E-5 4.762054426472451E-5 0.0 9.524108852944902E-5 4.762054426472451E-5 12-13 9.524108852944902E-5 4.762054426472451E-5 0.0 1.1905136066181127E-4 4.762054426472451E-5 14-15 1.4286163279417352E-4 4.762054426472451E-5 0.0 1.9048217705889804E-4 4.762054426472451E-5 16-17 1.4286163279417352E-4 4.762054426472451E-5 0.0 2.142924491912603E-4 4.762054426472451E-5 18-19 1.4286163279417352E-4 4.762054426472451E-5 0.0 2.8572326558834704E-4 4.762054426472451E-5 20-21 1.4286163279417352E-4 4.762054426472451E-5 0.0 2.8572326558834704E-4 4.762054426472451E-5 22-23 1.4286163279417352E-4 4.762054426472451E-5 0.0 3.809643541177961E-4 4.762054426472451E-5 24-25 1.4286163279417352E-4 4.762054426472451E-5 0.0 4.0477462625015834E-4 4.762054426472451E-5 26-27 1.4286163279417352E-4 4.762054426472451E-5 0.0 5.000157147796074E-4 4.762054426472451E-5 28-29 1.666719049265358E-4 4.762054426472451E-5 0.0 6.666876197061432E-4 4.762054426472451E-5 30-31 2.3810272132362254E-4 4.762054426472451E-5 0.0 0.0012857546951475618 4.762054426472451E-5 32-33 3.095335377207093E-4 4.762054426472451E-5 0.0 0.0020953039476478783 7.143081639708677E-5 34-35 3.809643541177961E-4 4.762054426472451E-5 0.0 0.0029286634722805573 1.4286163279417352E-4 36-37 4.285848983825206E-4 4.762054426472451E-5 0.0 0.004190607895295757 1.4286163279417352E-4 38-39 4.762054426472451E-4 4.762054426472451E-5 0.0 0.0057858961281640284 1.4286163279417352E-4 40-41 4.762054426472451E-4 4.762054426472451E-5 0.0 0.008000251436473718 1.4286163279417352E-4 42-43 4.762054426472451E-4 4.762054426472451E-5 0.0 0.011381310079269158 1.4286163279417352E-4 44-45 5.238259869119696E-4 4.762054426472451E-5 0.0 0.01869106362390437 1.4286163279417352E-4 46-47 5.476362590443318E-4 4.762054426472451E-5 0.0 0.0321676776508214 1.4286163279417352E-4 48-49 5.714465311766941E-4 4.762054426472451E-5 0.0 0.043215643920237495 1.4286163279417352E-4 50-51 5.714465311766941E-4 4.762054426472451E-5 0.0 0.05578746760612476 1.4286163279417352E-4 52-53 5.714465311766941E-4 4.762054426472451E-5 0.0 0.07143081639708676 1.9048217705889804E-4 54-55 5.714465311766941E-4 4.762054426472451E-5 0.0 0.09047903410297657 2.619129934559848E-4 56-57 5.714465311766941E-4 4.762054426472451E-5 0.0 0.1132892748057796 2.8572326558834704E-4 58-59 5.714465311766941E-4 4.762054426472451E-5 0.0 0.14745701531571945 2.8572326558834704E-4 60-61 6.190670754414187E-4 4.762054426472451E-5 0.0 0.18588679453735213 2.8572326558834704E-4 62-63 6.190670754414187E-4 4.762054426472451E-5 0.0 0.23234063546759087 2.8572326558834704E-4 64-65 6.190670754414187E-4 4.762054426472451E-5 0.0 0.27391337061069543 2.8572326558834704E-4 66-67 6.190670754414187E-4 4.762054426472451E-5 0.0 0.33234377842351237 2.8572326558834704E-4 68-69 6.666876197061432E-4 4.762054426472451E-5 0.0 0.4087271314241304 2.8572326558834704E-4 70-71 6.666876197061432E-4 4.762054426472451E-5 0.0 0.46741945223040343 2.8572326558834704E-4 72-73 6.666876197061432E-4 4.762054426472451E-5 0.0 0.5589223280350716 3.809643541177961E-4 74-75 6.666876197061432E-4 4.762054426472451E-5 0.0 0.6371628822620139 3.809643541177961E-4 76-77 6.666876197061432E-4 4.762054426472451E-5 0.0 0.7123795319281463 3.809643541177961E-4 78-79 7.143081639708677E-4 4.762054426472451E-5 0.0 0.8051919727000943 3.809643541177961E-4 80-81 7.619287082355922E-4 4.762054426472451E-5 0.0 0.9005759228623376 4.0477462625015834E-4 82-83 8.095492525003167E-4 4.762054426472451E-5 0.0 1.0250322152981952 4.285848983825206E-4 84-85 8.33359524632679E-4 4.762054426472451E-5 0.0 1.1938470447166434 4.285848983825206E-4 86-87 8.571697967650412E-4 4.762054426472451E-5 0.0 1.403353629209299 4.285848983825206E-4 88-89 8.571697967650412E-4 4.762054426472451E-5 0.0 1.6528376606121906 4.5239517051488283E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4005 0.0 50.197884 1 GTATCAA 7245 0.0 42.115715 1 TCAACGC 9085 0.0 33.166645 4 TATCAAC 9205 0.0 32.994 2 ATCAACG 9140 0.0 32.965492 3 CAACGCA 9365 0.0 32.173473 5 AACGCAG 9780 0.0 30.905426 6 ACGCAGA 11030 0.0 27.186266 7 CGCAGAG 11215 0.0 26.56768 8 GTACATG 8470 0.0 25.307001 1 TACATGG 8720 0.0 24.145998 2 GCAGAGT 12300 0.0 23.683218 9 ACATGGG 9030 0.0 22.841177 3 CGCGGAA 250 0.0 21.854437 82-83 ATTCGCG 290 0.0 21.29489 80-81 GAGTACT 6365 0.0 21.198334 12-13 GTGGTAT 2180 0.0 20.711647 1 CATGGGG 5405 0.0 20.575829 4 GTATCGA 140 4.8344728E-4 20.369051 1 CTCGCGC 305 0.0 20.243254 60-61 >>END_MODULE