FastQCFastQC Report
Thu 26 May 2016
SRR936795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099934
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73720.3510586523195491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58080.27658012108951996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.17667221922212795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.050.2191051
GTATCAA75100.041.8802341
TCAACGC97200.032.3007434
ATCAACG97200.032.253413
TATCAAC97250.032.2375952
CAACGCA99700.031.44395
AACGCAG103650.030.291436
ACGCAGA117500.026.4373027
CGCAGAG119300.025.8393488
GTACATG86850.023.2493171
GCAGAGT131950.022.966179
TACATGG89100.022.3913292
GTGGTAT22250.021.7801671
TGGTATC20900.021.591642
GAGTACT70800.021.13167412-13
ACATGGG89200.021.0876563
AGTACTT73550.021.084212-13
AGAGTAC113750.020.7113110-11
GTACTTT73800.020.17660714-15
CAGAGTA124600.019.72738810-11
Produced by FastQC (version 0.11.5)