##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936795_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099934 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11632937035164 31.0 31.0 34.0 31.0 34.0 2 32.11218685920605 33.0 31.0 34.0 30.0 34.0 3 32.299907997108484 33.0 31.0 34.0 30.0 34.0 4 36.03670401069748 37.0 35.0 37.0 35.0 37.0 5 35.9535790172453 37.0 35.0 37.0 35.0 37.0 6 35.98976444021574 37.0 35.0 37.0 35.0 37.0 7 35.92918682206203 37.0 35.0 37.0 35.0 37.0 8 35.90438366158175 37.0 35.0 37.0 35.0 37.0 9 37.5903285531831 39.0 37.0 39.0 35.0 39.0 10-11 37.51213895293852 39.0 37.0 39.0 35.0 39.0 12-13 37.412927501531 39.0 37.0 39.0 34.0 39.0 14-15 38.63479923654744 40.0 38.0 41.0 34.0 41.0 16-17 38.53864359546538 40.0 38.0 41.0 34.0 41.0 18-19 38.57073698506715 40.0 38.0 41.0 34.0 41.0 20-21 38.64247900172101 40.0 38.0 41.0 34.0 41.0 22-23 38.57188868793019 40.0 38.0 41.0 34.0 41.0 24-25 38.468182571452246 40.0 38.0 41.0 34.0 41.0 26-27 38.30779538785505 40.0 38.0 41.0 34.0 41.0 28-29 38.13328966529424 40.0 38.0 41.0 33.0 41.0 30-31 38.020233254949915 40.0 37.0 41.0 33.0 41.0 32-33 38.04462735495497 40.0 37.0 41.0 33.0 41.0 34-35 37.97821764874515 40.0 37.0 41.0 33.0 41.0 36-37 37.83538268345576 40.0 37.0 41.0 33.0 41.0 38-39 37.68300456109573 40.0 37.0 41.0 33.0 41.0 40-41 37.50970982897557 40.0 37.0 41.0 32.5 41.0 42-43 37.294793788757175 40.0 36.5 41.0 32.0 41.0 44-45 36.97782906510395 39.5 36.0 41.0 31.0 41.0 46-47 36.72892600434109 39.0 36.0 41.0 31.0 41.0 48-49 36.41946151640956 39.0 35.5 41.0 30.0 41.0 50-51 36.15846402791707 39.0 35.0 40.0 30.0 41.0 52-53 35.88134246123926 39.0 35.0 40.0 29.0 41.0 54-55 35.624894401443086 38.0 35.0 40.0 28.0 41.0 56-57 35.32977465006043 38.0 34.5 40.0 28.0 41.0 58-59 34.97531589088038 38.0 34.0 40.0 27.0 41.0 60-61 34.61888683168138 37.0 34.0 40.0 26.5 41.0 62-63 34.22379203346391 37.0 33.5 39.5 26.0 41.0 64-65 33.79796412649159 36.0 33.0 39.0 26.0 40.5 66-67 33.39953565207287 36.0 32.5 39.0 25.5 40.0 68-69 32.9496562749115 35.0 32.0 38.0 24.5 40.0 70-71 32.46682776696792 35.0 31.5 37.5 23.5 40.0 72-73 32.11630198853869 35.0 31.0 37.0 22.5 39.0 74-75 31.67014463311704 35.0 31.0 36.5 21.5 39.0 76-77 30.303434536514004 33.5 29.5 35.0 20.0 37.0 78-79 31.037209026569407 34.0 31.0 35.5 20.5 37.5 80-81 30.89121848591432 34.0 31.0 35.0 20.0 37.0 82-83 30.572631806523443 34.0 31.0 35.0 19.0 37.0 84-85 30.207695098988825 34.0 30.0 35.0 18.0 36.0 86-87 29.85599642655436 34.0 30.0 35.0 14.5 36.0 88-89 29.502634844714166 34.0 29.5 35.0 8.5 35.0 90-91 29.086111515885737 34.0 29.0 35.0 4.5 35.0 92-93 28.622955530983354 34.0 29.0 35.0 2.0 35.0 94-95 28.13018099616464 33.0 28.5 35.0 2.0 35.0 96-97 27.476119487564848 33.0 27.0 35.0 2.0 35.0 98-99 26.7136876682791 33.0 25.5 34.5 2.0 35.0 100-101 23.672415418770306 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.307381523120803 1101 2 0.2117481370116323 1101 3 0.28106004253550765 1101 4 0.24843806855193407 1101 5 0.2817727416760363 1101 6 0.24290774183203467 1101 7 0.28979579919218423 1101 8 0.28524176239411503 1101 9 0.3090150008087704 1101 10-11 0.32186445277707776 1101 12-13 0.3819091615864778 1101 14-15 0.49208695363655863 1101 16-17 0.4564344673070053 1101 18-19 0.3949369281920454 1101 20-21 0.4089857194449209 1101 22-23 0.27385745477352685 1101 24-25 0.449630073158076 1101 26-27 0.36366550209410065 1101 28-29 0.3743961792453021 1101 30-31 0.47416859385565147 1101 32-33 0.48288356952341616 1101 34-35 0.5985785448483725 1101 36-37 0.6329923759137301 1101 38-39 0.558719250291162 1101 40-41 0.542974103223635 1101 42-43 0.5434916541602988 1101 44-45 0.6984561145294421 1101 46-47 0.7803500002515946 1101 48-49 0.6892062365733054 1101 50-51 0.74117351013291 1101 52-53 0.5716865449888573 1101 54-55 0.5987568900158493 1101 56-57 0.823654707525364 1101 58-59 0.8560176328701914 1101 60-61 0.7913065007888704 1101 62-63 0.7756767818513239 1101 64-65 0.7145832994483214 1101 66-67 0.8240541790426263 1101 68-69 0.7183071555446929 1101 70-71 0.6953123920008011 1101 72-73 0.7840201773727884 1101 74-75 0.7388089638764832 1101 76-77 0.747713007901389 1101 78-79 0.8605312901390505 1101 80-81 0.9128274597398764 1101 82-83 1.07271933570971 1101 84-85 1.0547151606151424 1101 86-87 0.9407780494477223 1101 88-89 1.0143998421398983 1101 90-91 1.0931185525317453 1101 92-93 1.0830900594709192 1101 94-95 1.0995590501575094 1101 96-97 1.1890349249013212 1101 98-99 1.3873050830883606 1101 100-101 1.4548687839527226 1102 1 0.268855092031977 1102 2 0.12130177650461604 1102 3 0.18906904729304586 1102 4 0.20914037229888294 1102 5 0.21819018868312412 1102 6 0.20635366620069817 1102 7 0.2592782150303705 1102 8 0.27303409740729023 1102 9 0.3981582072831884 1102 10-11 0.36793018498182306 1102 12-13 0.3487113520637237 1102 14-15 0.48525920513634446 1102 16-17 0.44662056527872096 1102 18-19 0.4194466934296557 1102 20-21 0.3129250641220622 1102 22-23 0.3499070409155891 1102 24-25 0.4013978539504208 1102 26-27 0.4095205305483134 1102 28-29 0.2656264839686955 1102 30-31 0.377472407619841 1102 32-33 0.40778254483503673 1102 34-35 0.43767321843643714 1102 36-37 0.4696806601012682 1102 38-39 0.5314285112076362 1102 40-41 0.6071377355984637 1102 42-43 0.5759326663169375 1102 44-45 0.605934808723859 1102 46-47 0.6658229104833779 1102 48-49 0.5992225825546527 1102 50-51 0.5625188259596854 1102 52-53 0.6407160924413873 1102 54-55 0.6697080241070879 1102 56-57 0.5979817839305923 1102 58-59 0.6202151461976655 1102 60-61 0.56592240705438 1102 62-63 0.6073113326367192 1102 64-65 0.5548069873900161 1102 66-67 0.5648617347055094 1102 68-69 0.5785558543635432 1102 70-71 0.6058145929474748 1102 72-73 0.7938193485520983 1102 74-75 0.5489262161733102 1102 76-77 0.5736304109789785 1102 78-79 0.7207599970914309 1102 80-81 0.8006312637711837 1102 82-83 0.8847131929456857 1102 84-85 0.7251158344462603 1102 86-87 0.7023282187735695 1102 88-89 0.7964211566261632 1102 90-91 0.932402096647742 1102 92-93 0.972930403373109 1102 94-95 0.9101421655531894 1102 96-97 0.9517522509576146 1102 98-99 1.0432113906800602 1102 100-101 1.1232700080862728 1103 1 0.011164358370727712 1103 2 -0.14360072570330829 1103 3 0.013866031446880811 1103 4 0.09334351731774149 1103 5 0.05547691361029905 1103 6 0.04683676863567854 1103 7 0.18351937717763178 1103 8 0.22676129881443075 1103 9 0.1802464555168939 1103 10-11 0.2252742908696348 1103 12-13 0.25052397784111236 1103 14-15 0.2767770602004376 1103 16-17 0.30768032636269993 1103 18-19 0.3008062282975814 1103 20-21 0.265894820292651 1103 22-23 0.2433268044071042 1103 24-25 0.3329553817609252 1103 26-27 0.300916301030675 1103 28-29 0.3249426206047801 1103 30-31 0.3710543871836478 1103 32-33 0.39020902203002095 1103 34-35 0.4332764316460569 1103 36-37 0.3932144767026031 1103 38-39 0.3859303866261783 1103 40-41 0.41895708904859674 1103 42-43 0.5211574932056848 1103 44-45 0.5597885096940658 1103 46-47 0.5713661092649787 1103 48-49 0.6168312010121397 1103 50-51 0.36636637872344835 1103 52-53 0.38243554486643205 1103 54-55 0.4086729420789723 1103 56-57 0.4611504746765007 1103 58-59 0.41513719332103705 1103 60-61 0.5182864829720515 1103 62-63 0.5089508945946832 1103 64-65 0.2961871243544678 1103 66-67 0.5318373293440004 1103 68-69 0.4702356668096215 1103 70-71 0.4225187955785543 1103 72-73 0.46611572004103863 1103 74-75 0.4307342338139186 1103 76-77 0.33332125191047624 1103 78-79 0.3323531344858246 1103 80-81 0.45365770761236135 1103 82-83 0.4047244408004964 1103 84-85 0.3421707866497776 1103 86-87 0.11442361203372187 1103 88-89 0.35626872939532106 1103 90-91 0.6069593352424754 1103 92-93 0.6152969092551253 1103 94-95 0.4415019849804551 1103 96-97 0.7423902140369876 1103 98-99 0.7819136517541203 1103 100-101 1.0604320615285872 1104 1 0.09417809692763512 1104 2 0.038155387464293256 1104 3 0.1067419075559144 1104 4 0.10518665908045222 1104 5 0.08295651859097575 1104 6 0.10466436877710805 1104 7 0.12332499925797435 1104 8 0.16837817050041082 1104 9 0.11163000714978466 1104 10-11 0.18505864202180078 1104 12-13 0.1475320440318768 1104 14-15 0.20827289187077014 1104 16-17 0.17643795219685643 1104 18-19 0.21157544975918796 1104 20-21 0.08200253937618385 1104 22-23 0.17490404355309153 1104 24-25 0.08625078306597089 1104 26-27 0.18829203896942914 1104 28-29 0.2976742302111859 1104 30-31 0.28429999736337663 1104 32-33 0.2742902038779462 1104 34-35 0.2177976943000317 1104 36-37 0.15508017233971572 1104 38-39 0.1393905602207255 1104 40-41 0.37398670980363846 1104 42-43 0.3430758376819085 1104 44-45 0.44698141444980877 1104 46-47 0.36292017708532853 1104 48-49 0.19758109998679174 1104 50-51 0.15188650665507453 1104 52-53 0.17515538553198695 1104 54-55 0.15094230135498776 1104 56-57 0.21987410866351098 1104 58-59 0.08277974136969135 1104 60-61 0.14080286544803045 1104 62-63 0.2134700504409821 1104 64-65 0.06222132759414478 1104 66-67 0.2190868901439984 1104 68-69 0.17958072898802868 1104 70-71 0.26290622754548565 1104 72-73 0.26352374160450154 1104 74-75 0.1862210531873707 1104 76-77 0.23318747674066742 1104 78-79 0.27557778157329693 1104 80-81 0.3273076113380107 1104 82-83 0.414071353553215 1104 84-85 0.4518841309098036 1104 86-87 0.3650771405718025 1104 88-89 0.3369320072570936 1104 90-91 0.33737033889967094 1104 92-93 0.692842467636563 1104 94-95 0.6020411747171721 1104 96-97 0.5492234418816544 1104 98-99 0.8339304485630201 1104 100-101 0.7129853113594962 1105 1 0.14308318813789356 1105 2 0.006756261080887782 1105 3 0.0931688076500663 1105 4 0.12870451864851162 1105 5 0.12273957857411233 1105 6 0.054091886146075296 1105 7 0.10804484295236705 1105 8 0.1857390017175149 1105 9 0.23001589475239115 1105 10-11 0.22728941506299805 1105 12-13 0.27084891000136224 1105 14-15 0.2376066061341362 1105 16-17 0.13275045081452674 1105 18-19 0.1413747431376393 1105 20-21 0.16039781131068054 1105 22-23 0.18369239542933968 1105 24-25 0.19097601534829067 1105 26-27 0.168411476253155 1105 28-29 0.17554512015622237 1105 30-31 0.2516861538470181 1105 32-33 0.15673380786352453 1105 34-35 0.30876347439043883 1105 36-37 0.13944211728362177 1105 38-39 0.051764681033674265 1105 40-41 -0.058749254323139155 1105 42-43 0.06287666830677807 1105 44-45 0.27732497714359994 1105 46-47 0.3278880493662868 1105 48-49 0.2918816629187546 1105 50-51 0.16086093663550827 1105 52-53 0.20105972420437723 1105 54-55 0.17632331820785652 1105 56-57 0.233375281522207 1105 58-59 0.23562882928537476 1105 60-61 0.157027395550557 1105 62-63 0.045819710574257044 1105 64-65 -0.006636801743297838 1105 66-67 -0.06803966727763822 1105 68-69 -0.047866577366903584 1105 70-71 -0.07158817466581269 1105 72-73 0.016230510844437163 1105 74-75 0.07292160507894252 1105 76-77 -0.07797605794635842 1105 78-79 -0.028188874290602683 1105 80-81 0.10911778206010325 1105 82-83 0.09009822410883928 1105 84-85 0.1140262481532659 1105 86-87 0.10774099303562679 1105 88-89 0.159628545857057 1105 90-91 0.3017440590414928 1105 92-93 0.34425264903346786 1105 94-95 0.48433343030367126 1105 96-97 0.6274138999500138 1105 98-99 0.6847872943636766 1105 100-101 0.6480426820905087 1106 1 0.40767904952230083 1106 2 0.21841844599438787 1106 3 0.2819280572826486 1106 4 0.19198238092712927 1106 5 0.180602541751 1106 6 0.19733834406358852 1106 7 0.30630883211914295 1106 8 0.3048952341354578 1106 9 0.4220826568301277 1106 10-11 0.37672122716736567 1106 12-13 0.37142458531278066 1106 14-15 0.6074121044644443 1106 16-17 0.6462203118266956 1106 18-19 0.6279346178624721 1106 20-21 0.5514619014506152 1106 22-23 0.445161298052291 1106 24-25 0.5060785608557339 1106 26-27 0.5513098976919792 1106 28-29 0.608761008024679 1106 30-31 0.6174975437039194 1106 32-33 0.5652814417331768 1106 34-35 0.49431650561724183 1106 36-37 0.5098478924343866 1106 38-39 0.5119270296006846 1106 40-41 0.5895999844206017 1106 42-43 0.542854248844634 1106 44-45 0.6140489332373917 1106 46-47 0.4156078317605889 1106 48-49 0.27657417614474156 1106 50-51 0.37440064454072797 1106 52-53 0.4475194600911294 1106 54-55 0.5708874543419071 1106 56-57 0.44013459961116297 1106 58-59 0.40324682561012537 1106 60-61 0.5035347300091431 1106 62-63 0.4676612511849285 1106 64-65 0.6186782580169776 1106 66-67 0.4521028717264457 1106 68-69 0.5657689694604642 1106 70-71 0.6059356727534961 1106 72-73 0.6415468541617031 1106 74-75 0.6023164507419132 1106 76-77 0.506125805136616 1106 78-79 0.6607468644285355 1106 80-81 0.48219356968276017 1106 82-83 0.5689549385024328 1106 84-85 0.5244959016570547 1106 86-87 0.540444099885331 1106 88-89 0.8939218728581757 1106 90-91 1.052812513275196 1106 92-93 1.1699381219493468 1106 94-95 1.0840505129497657 1106 96-97 1.5502806817089585 1106 98-99 1.484278043126647 1106 100-101 1.602684558993115 1107 1 0.1480900564236478 1107 2 0.051475092248601584 1107 3 0.02162315897193423 1107 4 0.08476817223182564 1107 5 0.1027103818964008 1107 6 0.1224386544323437 1107 7 0.1308523775566357 1107 8 0.08356614152892661 1107 9 0.12320500270254087 1107 10-11 0.11044755109469406 1107 12-13 0.1186935436899148 1107 14-15 0.10748749265144397 1107 16-17 0.19166345195377943 1107 18-19 0.13358522808859163 1107 20-21 0.13060909942880983 1107 22-23 0.26093087920139624 1107 24-25 0.22583795160163334 1107 26-27 0.18330195424103124 1107 28-29 0.19372496410738904 1107 30-31 0.3679178865336823 1107 32-33 0.23922131654583723 1107 34-35 0.337344850219246 1107 36-37 0.30491782147681334 1107 38-39 0.2766221878028574 1107 40-41 0.38691200561519423 1107 42-43 0.4203343167505196 1107 44-45 0.6481382959302948 1107 46-47 0.5248587647883056 1107 48-49 0.4823477584060285 1107 50-51 0.484104697870805 1107 52-53 0.6149209072730528 1107 54-55 0.5207191589739253 1107 56-57 0.5204429113000586 1107 58-59 0.48047416789961517 1107 60-61 0.6840145017875088 1107 62-63 0.5052813128065807 1107 64-65 0.46524114442382114 1107 66-67 0.2773592706802859 1107 68-69 0.4916368378170901 1107 70-71 0.47480679399171066 1107 72-73 0.5122086426362458 1107 74-75 0.588535105870907 1107 76-77 0.6366479615778431 1107 78-79 0.6341939468943956 1107 80-81 0.5565689327780419 1107 82-83 0.6619653120073856 1107 84-85 0.7160448519867515 1107 86-87 0.8543969048458031 1107 88-89 0.8427911630789602 1107 90-91 0.8547056948827425 1107 92-93 0.9657118624467067 1107 94-95 0.8851388380661263 1107 96-97 0.9613926970262092 1107 98-99 0.8433490391323168 1107 100-101 0.9142296890518651 1108 1 -0.025906777091222466 1108 2 -0.13920284901236002 1108 3 -0.08151077021714315 1108 4 0.023397492977387913 1108 5 0.09196692229839698 1108 6 0.05054748271950871 1108 7 -0.01689842920921336 1108 8 0.10784220181486859 1108 9 -0.004885808432760541 1108 10-11 0.03502818332085411 1108 12-13 0.09436902078292064 1108 14-15 0.08029869205050488 1108 16-17 0.0667787949460319 1108 18-19 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68-69 -0.9952944718227599 2316 70-71 -0.9178236952336647 2316 72-73 -0.785916038836028 2316 74-75 -0.750070422076142 2316 76-77 -1.1591045811621825 2316 78-79 -0.9176455902129632 2316 80-81 -0.7790853755109559 2316 82-83 -0.674706623863802 2316 84-85 -0.6207108064279261 2316 86-87 -0.6719534367886872 2316 88-89 -0.9136396917348542 2316 90-91 -0.7771430251258131 2316 92-93 -1.2870056317774363 2316 94-95 -1.1178097237334015 2316 96-97 -0.8134421669013996 2316 98-99 -0.8137921948183724 2316 100-101 -1.0747800659533802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 17.0 5 41.0 6 77.0 7 250.0 8 522.0 9 1070.0 10 1683.0 11 2666.0 12 4062.0 13 5617.0 14 7189.0 15 9862.0 16 12822.0 17 17488.0 18 16138.0 19 11002.0 20 10106.0 21 10570.0 22 11515.0 23 12581.0 24 14751.0 25 17447.0 26 21659.0 27 26919.0 28 33929.0 29 42386.0 30 52777.0 31 66779.0 32 84823.0 33 110369.0 34 151223.0 35 213885.0 36 328477.0 37 470269.0 38 307709.0 39 21246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27600634980208 18.884707655245563 14.762670669982231 23.076615324970128 2 16.033264789668735 20.263605790431637 39.136654605648516 24.566474814251112 3 19.83469460743065 23.96704368982666 29.218032917260157 26.980228785482534 4 13.938029392394322 16.74997892787906 35.52254812181924 33.789443557907376 5 14.851728441918818 36.74713569284589 33.17362708742381 15.227508777811483 6 33.163660606926435 35.20078098027311 16.865554722192456 14.770003690607997 7 29.340745633313066 32.16755010755105 20.052592154130632 18.439112105005254 8 27.086327475053977 34.61123063867721 18.995216040123168 19.30722584614564 9 26.73064962994075 16.561330022753097 19.615711731892528 37.09230861541363 10-11 25.62436515831696 26.2831242711077 26.503726547466037 21.58878402310931 12-13 26.48376591758204 23.359811345059345 27.36622873697241 22.790194000386204 14-15 23.4748806391058 24.733158280212617 25.380464338403012 26.41149674227857 16-17 23.080701583954543 27.974736348856677 25.880503863454756 23.06405820373402 18-19 23.046533843444603 26.546572416085457 28.177457005791613 22.22943673467833 20-21 23.688925684458308 25.658099791393884 28.39081371147686 22.26216081267095 22-23 23.502333882874417 25.48032461972614 28.314389880824827 22.702951616574616 24-25 23.24148572163316 25.72839553207245 28.354410185465184 22.6757085608292 26-27 23.232885310292147 25.938167296222854 28.152805001400072 22.676142392084927 28-29 22.981737085367502 25.902103310392878 28.418156193054145 22.69800341118548 30-31 23.195865486374984 25.753336121173902 28.27048943748172 22.78030895496939 32-33 23.055902129604938 25.882581241190138 28.26145757933636 22.800059049868565 34-35 23.38325362246718 25.88227618612674 28.092972887511248 22.641497303894838 36-37 23.429060035016796 25.774275727855734 28.010031195092793 22.78663304203468 38-39 23.257878299828278 25.8786207986925 28.102626719025164 22.76087418245406 40-41 23.381967933309284 25.798768240951404 28.08058223414375 22.73868159159556 42-43 23.2736355486557 25.92737927003059 27.83696230622428 22.96202287508944 44-45 23.387886933922562 26.056699711536975 27.67940245413819 22.876010900402278 46-47 23.521179009044573 26.18826410175944 27.369944660139154 22.920612229056832 48-49 23.570963589721366 26.340176263102226 27.126083786642308 22.962776360534093 50-51 23.46162802492082 26.454988282948342 27.025149873972047 23.058233818158786 52-53 23.67911821129775 26.50332978001237 26.825938306506437 22.991613702183447 54-55 23.53029417332284 26.414619017628493 26.85152753685649 23.203559272192173 56-57 23.447892104795095 26.74179310561584 26.813081314988878 22.997233474600183 58-59 23.600126195734347 26.507794967647463 26.84164221128261 23.05043662533558 60-61 23.546773820261212 26.35753178463499 27.132706387023468 22.96298800808033 62-63 23.452879947655497 26.478070263160653 27.14330545626672 22.92574433291713 64-65 23.486801110798666 26.39975465854703 27.01870371424197 23.09474051641233 66-67 23.389693054565775 26.614176115576992 26.88447038918137 23.11166044067586 68-69 23.31287554751721 26.481546562891978 27.030278094454395 23.17529979513642 70-71 23.331129292136097 26.418564804882827 26.898604501434694 23.351701401546386 72-73 23.39666946596166 26.565222184416793 26.77489549073816 23.26321285888339 74-75 23.399663464251468 26.738853354559485 26.528631610978582 23.332851570210465 76-77 23.51914107265438 26.653710626802003 26.545254191644784 23.281894108898832 78-79 23.42819685808425 26.69104526867337 26.566279145628258 23.314478727614123 80-81 23.37742369392754 26.79869883868424 26.521746066858697 23.30213140052952 82-83 23.48871790170711 26.718685078676458 26.506650919866324 23.285946099750106 84-85 23.460380601292037 26.75795522704712 26.349285417157276 23.43237875450357 86-87 23.437687137644968 26.640328797940278 26.55880077613534 23.36318328827942 88-89 23.441145993062555 26.507375561215063 26.496494132123882 23.5549843135985 90-91 23.417609851745237 26.64699936783682 26.514114144145108 23.421276636272832 92-93 23.446152805827612 26.584181495314375 26.440200711165545 23.529464987692464 94-95 23.419448820247514 26.542049933045455 26.403902602560464 23.634598644146575 96-97 23.38308256327627 26.482208544841573 26.39065650077849 23.744052391103665 98-99 23.224657074989192 26.566354394259474 26.332888227685824 23.876100303065513 100-101 23.130663321770967 26.72968823514845 26.22923964577631 23.91040879730427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 327.0 1 248.0 2 195.5 3 226.5 4 213.5 5 230.0 6 398.0 7 847.5 8 1443.5 9 1810.5 10 1754.0 11 1671.5 12 1786.5 13 1945.5 14 2125.5 15 2297.0 16 2456.0 17 2638.0 18 2821.5 19 3024.5 20 3293.5 21 3711.5 22 4342.5 23 5257.5 24 6352.0 25 7616.0 26 9600.5 27 11887.0 28 13848.0 29 16299.5 30 19019.0 31 21678.0 32 24631.5 33 27565.0 34 31021.5 35 34170.5 36 37994.0 37 42197.0 38 45916.5 39 49171.5 40 51904.0 41 55257.5 42 59420.5 43 63728.5 44 67478.0 45 71905.0 46 75750.5 47 79146.0 48 84125.0 49 86427.5 50 85092.0 51 83944.0 52 83861.5 53 84088.0 54 83192.0 55 79787.0 56 74284.0 57 67386.0 58 61172.5 59 55246.5 60 47547.0 61 39347.5 62 31411.0 63 24134.5 64 17903.5 65 12955.0 66 9054.0 67 6839.0 68 4981.5 69 3239.5 70 2200.0 71 1314.0 72 785.5 73 488.0 74 288.0 75 163.0 76 95.5 77 48.0 78 25.5 79 17.5 80 10.5 81 6.5 82 4.0 83 4.5 84 4.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01623860559427106 2 0.0026667504788245727 3 0.0030000942886776443 4 1.4286163279417352E-4 5 0.001761960137794807 6 4.285848983825206E-4 7 4.762054426472451E-5 8 0.0 9 0.0 10-11 4.762054426472451E-5 12-13 7.381184361032299E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002071493675515516 22-23 0.0 24-25 0.0037620229969132363 26-27 0.0020000628591184295 28-29 0.0036905921805161493 30-31 0.016190985050006334 32-33 6.666876197061432E-4 34-35 0.0025238888460303987 36-37 0.004023935990369221 38-39 0.0024762683017656747 40-41 0.0043572798002222924 42-43 0.002452458029633312 44-45 5.476362590443319E-4 46-47 4.762054426472451E-5 48-49 2.3810272132362257E-4 50-51 1.4286163279417352E-4 52-53 9.762211574268524E-4 54-55 0.00316676619360418 56-57 3.5715408198543384E-4 58-59 0.001023841701691577 60-61 9.524108852944903E-4 62-63 0.0 64-65 1.666719049265358E-4 66-67 2.3810272132362255E-5 68-69 0.0 70-71 1.666719049265358E-4 72-73 2.3810272132362255E-5 74-75 4.047746262501584E-4 76-77 5.952568033090564E-4 78-79 2.3810272132362257E-4 80-81 0.00559541395110513 82-83 0.0017381498656624448 84-85 0.003452489459192527 86-87 0.0020238731312507917 88-89 0.0012381341508828374 90-91 7.143081639708676E-5 92-93 4.762054426472451E-5 94-95 9.524108852944902E-5 96-97 0.0016905293213977201 98-99 0.002761991567354022 100-101 8.571697967650412E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2099934.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.49735316868081 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9645959249997 43.67763200215839 2 13.496360903130942 15.520100586785912 3 4.3199368886763665 7.451548108639115 4 2.0311741845589277 4.671485577467677 5 1.1058125370531386 3.1790646990649623 6 0.707035694238909 2.439160860871078 7 0.4515825584305946 1.8175361292830237 8 0.3333240179387438 1.533219902322212 9 0.24464556397855705 1.2659825153903572 >10 1.261514495942796 12.838364054967693 >50 0.06033444768311479 2.3486569034877998 >100 0.022769487779673955 2.1535879735204966 >500 5.811881018966367E-4 0.21916632248869264 >1k 1.6605374339902855E-4 0.2553041804986862 >5k 1.6605374339902855E-4 0.6291901830539798 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7372 0.3510586523195491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5808 0.27658012108951996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3710 0.17667221922212795 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.524108852944902E-5 2 4.762054426472451E-5 0.0 0.0 0.0 9.524108852944902E-5 3 4.762054426472451E-5 0.0 0.0 0.0 9.524108852944902E-5 4 4.762054426472451E-5 0.0 0.0 0.0 9.524108852944902E-5 5 4.762054426472451E-5 0.0 0.0 4.762054426472451E-5 9.524108852944902E-5 6 9.524108852944902E-5 4.762054426472451E-5 0.0 4.762054426472451E-5 9.524108852944902E-5 7 1.4286163279417352E-4 4.762054426472451E-5 0.0 4.762054426472451E-5 9.524108852944902E-5 8 1.4286163279417352E-4 4.762054426472451E-5 0.0 4.762054426472451E-5 9.524108852944902E-5 9 1.4286163279417352E-4 4.762054426472451E-5 0.0 4.762054426472451E-5 9.524108852944902E-5 10-11 1.9048217705889804E-4 4.762054426472451E-5 0.0 4.762054426472451E-5 9.524108852944902E-5 12-13 1.9048217705889804E-4 7.143081639708677E-5 0.0 4.762054426472451E-5 9.524108852944902E-5 14-15 2.142924491912603E-4 9.524108852944902E-5 0.0 9.524108852944902E-5 9.524108852944902E-5 16-17 2.857232655883471E-4 9.524108852944902E-5 0.0 9.524108852944902E-5 1.4286163279417352E-4 18-19 3.333438098530716E-4 1.4286163279417352E-4 0.0 9.524108852944902E-5 1.4286163279417352E-4 20-21 3.333438098530716E-4 1.4286163279417352E-4 0.0 9.524108852944902E-5 1.4286163279417352E-4 22-23 3.333438098530716E-4 1.9048217705889804E-4 0.0 1.4286163279417352E-4 1.4286163279417352E-4 24-25 3.333438098530716E-4 1.9048217705889804E-4 0.0 1.666719049265358E-4 1.4286163279417352E-4 26-27 3.333438098530716E-4 1.9048217705889804E-4 0.0 2.857232655883471E-4 1.4286163279417352E-4 28-29 4.285848983825206E-4 1.9048217705889804E-4 0.0 4.762054426472451E-4 1.4286163279417352E-4 30-31 4.762054426472451E-4 1.9048217705889804E-4 0.0 0.001119082790221026 1.4286163279417352E-4 32-33 4.762054426472451E-4 1.9048217705889804E-4 0.0 0.001857201226324256 2.3810272132362254E-4 34-35 5.000157147796074E-4 1.9048217705889804E-4 0.0 0.0027381812952216593 2.3810272132362254E-4 36-37 5.238259869119696E-4 1.9048217705889804E-4 0.0 0.0039763154461044965 2.3810272132362254E-4 38-39 5.238259869119696E-4 1.9048217705889804E-4 0.0 0.005595413951105129 2.3810272132362254E-4 40-41 5.238259869119696E-4 1.9048217705889804E-4 0.0 0.007476425449561748 2.3810272132362254E-4 42-43 5.476362590443318E-4 1.9048217705889804E-4 0.0 0.010786053275960102 2.3810272132362254E-4 44-45 6.666876197061432E-4 1.9048217705889804E-4 0.0 0.018024376004198227 2.3810272132362254E-4 46-47 7.3811843610323E-4 1.9048217705889804E-4 0.0 0.03140574894258581 2.3810272132362254E-4 48-49 7.619287082355922E-4 1.9048217705889804E-4 0.0 0.04242990493986954 2.3810272132362254E-4 50-51 7.619287082355922E-4 1.9048217705889804E-4 0.0 0.054858866992962635 2.3810272132362254E-4 52-53 7.619287082355922E-4 1.9048217705889804E-4 0.0 0.07059745687245408 2.619129934559848E-4 54-55 7.619287082355922E-4 1.9048217705889804E-4 0.0 0.08971710539474098 2.8572326558834704E-4 56-57 8.095492525003167E-4 1.9048217705889804E-4 0.0 0.11255115636967639 3.095335377207093E-4 58-59 8.095492525003167E-4 1.9048217705889804E-4 0.0 0.14698080987307222 3.333438098530716E-4 60-61 8.33359524632679E-4 1.9048217705889804E-4 0.0 0.1853391582783078 3.333438098530716E-4 62-63 8.571697967650412E-4 1.9048217705889804E-4 0.0 0.23143584512656112 3.809643541177961E-4 64-65 8.571697967650412E-4 1.9048217705889804E-4 0.0 0.2728657186368715 3.809643541177961E-4 66-67 8.571697967650412E-4 1.9048217705889804E-4 0.0 0.3311770750890266 3.809643541177961E-4 68-69 9.524108852944902E-4 1.9048217705889804E-4 0.0 0.40791758217163016 3.809643541177961E-4 70-71 0.001023841701691577 1.9048217705889804E-4 0.0 0.46691943651562384 3.809643541177961E-4 72-73 0.0010714622459563014 1.9048217705889804E-4 0.0 0.5585651739530861 4.762054426472451E-4 74-75 0.0010952725180886638 1.9048217705889804E-4 0.0 0.6369485898128227 4.762054426472451E-4 76-77 0.0010952725180886638 1.9048217705889804E-4 0.0 0.7121176189346903 4.762054426472451E-4 78-79 0.0010952725180886638 1.9048217705889804E-4 0.0 0.8049062494345061 4.762054426472451E-4 80-81 0.0011428930623533882 1.9048217705889804E-4 0.0 0.9001949585082198 4.762054426472451E-4 82-83 0.0012381341508828374 1.9048217705889804E-4 0.0 1.0241988557735624 5.238259869119696E-4 84-85 0.0012381341508828374 1.9048217705889804E-4 0.0 1.1914183969591425 5.238259869119696E-4 86-87 0.0012381341508828374 1.9048217705889804E-4 0.0 1.4001868630156946 5.238259869119696E-4 88-89 0.0012381341508828374 1.9048217705889804E-4 0.0 1.6491946889759392 5.238259869119696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4210 0.0 50.219105 1 GTATCAA 7510 0.0 41.880234 1 TCAACGC 9720 0.0 32.300743 4 ATCAACG 9720 0.0 32.25341 3 TATCAAC 9725 0.0 32.237595 2 CAACGCA 9970 0.0 31.4439 5 AACGCAG 10365 0.0 30.29143 6 ACGCAGA 11750 0.0 26.437302 7 CGCAGAG 11930 0.0 25.839348 8 GTACATG 8685 0.0 23.249317 1 GCAGAGT 13195 0.0 22.96617 9 TACATGG 8910 0.0 22.391329 2 GTGGTAT 2225 0.0 21.780167 1 TGGTATC 2090 0.0 21.59164 2 GAGTACT 7080 0.0 21.131674 12-13 ACATGGG 8920 0.0 21.087656 3 AGTACTT 7355 0.0 21.0842 12-13 AGAGTAC 11375 0.0 20.71131 10-11 GTACTTT 7380 0.0 20.176607 14-15 CAGAGTA 12460 0.0 19.727388 10-11 >>END_MODULE