FastQCFastQC Report
Thu 26 May 2016
SRR936794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885065
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64110.3400943734035696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47860.253890449400949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29470.15633413171429103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.047.5191041
GTATCAA63350.045.1562771
ATCAACG79250.035.9166453
TCAACGC80200.035.373644
TATCAAC81900.034.698332
CAACGCA81750.034.6484955
AACGCAG85250.033.2799576
ACGCAGA96100.029.2258327
CGCAGAG97300.028.574628
GCAGAGT107050.025.0397439
GTACATG72400.023.890821
TACATGG74350.022.818052
GAGTACT55200.022.21148912-13
AGAGTAC92600.021.99175810-11
AGTACTT57700.021.9903712-13
ACATGGG77400.021.2423883
TGGTATC18800.020.4747432
GTACTTT59950.020.37072214-15
CAGAGTA100800.020.08487710-11
GTGGTAT19500.019.9823911
Produced by FastQC (version 0.11.5)