##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936794_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1885065 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71132825658532 33.0 31.0 34.0 30.0 34.0 2 31.837885165763513 33.0 31.0 34.0 30.0 34.0 3 31.856686108967065 33.0 31.0 34.0 30.0 34.0 4 35.42471161471886 37.0 35.0 37.0 33.0 37.0 5 35.420633771249264 37.0 35.0 37.0 33.0 37.0 6 35.471659067459214 37.0 35.0 37.0 33.0 37.0 7 35.40545233188246 37.0 35.0 37.0 33.0 37.0 8 35.3543352616488 37.0 35.0 37.0 33.0 37.0 9 36.984927310198856 39.0 37.0 39.0 33.0 39.0 10-11 36.809540785065764 39.0 37.0 39.0 33.0 39.0 12-13 36.735787360117556 39.0 37.0 39.0 32.5 39.0 14-15 37.91314543530329 40.0 38.0 41.0 33.0 41.0 16-17 37.83616029155493 40.0 38.0 41.0 33.0 41.0 18-19 37.77660160259725 40.0 38.0 41.0 32.0 41.0 20-21 37.68882770620642 40.0 37.5 41.0 32.0 41.0 22-23 37.57163678706039 40.0 37.0 41.0 32.0 41.0 24-25 37.41684636869286 40.0 37.0 41.0 32.0 41.0 26-27 37.511072562484586 40.0 37.0 41.0 32.0 41.0 28-29 37.461443769843484 40.0 37.0 41.0 32.0 41.0 30-31 37.32658025054839 40.0 37.0 41.0 31.5 41.0 32-33 37.13605366393201 40.0 36.5 41.0 31.0 41.0 34-35 36.97283197131134 40.0 36.0 41.0 30.5 41.0 36-37 36.80654141899616 39.0 36.0 41.0 30.0 41.0 38-39 36.648034948397004 39.0 36.0 41.0 30.0 41.0 40-41 36.4725627498256 39.0 35.5 41.0 30.0 41.0 42-43 36.26193181667476 39.0 35.0 41.0 30.0 41.0 44-45 36.01460931055428 39.0 35.0 40.5 29.0 41.0 46-47 35.68449045523629 39.0 35.0 40.0 27.5 41.0 48-49 35.37178320110978 39.0 35.0 40.0 26.5 41.0 50-51 34.739142151596894 38.0 34.0 39.5 25.5 40.5 52-53 34.96624068666067 38.5 34.5 40.0 26.5 40.5 54-55 35.32667520748622 39.0 35.0 40.0 27.0 41.0 56-57 35.19408057547088 39.0 35.0 40.5 26.0 41.0 58-59 34.93188802508136 38.0 34.5 40.0 26.0 41.0 60-61 34.6332044253116 38.0 34.0 40.0 26.0 41.0 62-63 34.29277027582603 37.5 34.0 40.0 25.5 41.0 64-65 33.94934445231331 37.0 34.0 40.0 24.5 41.0 66-67 33.565967221289455 36.0 33.0 39.0 23.5 41.0 68-69 33.16685976345643 36.0 33.0 39.0 22.5 41.0 70-71 32.74631272661685 35.0 32.5 38.5 22.0 40.0 72-73 32.35081336717832 35.0 32.0 37.5 21.0 40.0 74-75 31.92231753281717 35.0 32.0 37.0 20.0 39.0 76-77 31.498603231188312 35.0 31.5 36.5 18.5 39.0 78-79 31.074529525507078 35.0 31.0 36.0 17.5 38.5 80-81 30.67398922583571 35.0 31.0 35.5 13.0 37.0 82-83 30.291720709895944 34.0 30.5 35.0 9.0 37.0 84-85 29.95833724566527 34.0 30.0 35.0 7.0 36.5 86-87 29.64414569258885 34.0 30.0 35.0 4.0 36.0 88-89 29.313264264096993 34.0 29.5 35.0 2.0 36.0 90-91 28.97976223631546 34.0 29.0 35.0 2.0 35.0 92-93 28.622783034006787 34.0 29.0 35.0 2.0 35.0 94-95 28.206404818931972 34.0 29.0 35.0 2.0 35.0 96-97 27.738672141278947 34.0 28.0 35.0 2.0 35.0 98-99 27.139430735810173 34.0 27.0 35.0 2.0 35.0 100-101 24.94568304010737 31.5 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3453485478457452 1101 2 0.4295712599802748 1101 3 0.282082381410266 1101 4 0.24856966602646935 1101 5 0.21059708830707535 1101 6 0.05553818466216853 1101 7 0.11493735291309548 1101 8 0.21310097125837757 1101 9 0.12371674199793858 1101 10-11 0.16313459976145595 1101 12-13 0.27636667265326054 1101 14-15 0.5544021932435612 1101 16-17 0.531310855143829 1101 18-19 0.5004955093552823 1101 20-21 0.4805954021623009 1101 22-23 0.5219282362223154 1101 24-25 0.4921489024178669 1101 26-27 0.614902912977108 1101 28-29 0.44568915846924284 1101 30-31 0.39553793301843143 1101 32-33 0.4204859233988074 1101 34-35 0.3987498223621486 1101 36-37 0.37512799112745654 1101 38-39 0.45321127091876434 1101 40-41 0.3944894499079368 1101 42-43 0.2671301829411661 1101 44-45 0.5029532652113673 1101 46-47 0.4436339570350114 1101 48-49 0.5461141018208977 1101 50-51 0.3006562582004193 1101 52-53 0.38284064146841956 1101 54-55 0.42295964333013814 1101 56-57 0.3695046825034112 1101 58-59 0.31817510531798376 1101 60-61 0.43114811035537315 1101 62-63 0.3391440957268017 1101 64-65 0.4909161262349997 1101 66-67 0.459729447479269 1101 68-69 0.545235734125221 1101 70-71 0.3574326719439185 1101 72-73 0.40356605399465195 1101 74-75 0.34809469973162166 1101 76-77 0.33018001540821373 1101 78-79 0.3193499636114048 1101 80-81 0.03449131196596511 1101 82-83 0.2744731924278341 1101 84-85 0.25455695832746983 1101 86-87 0.16553111125969266 1101 88-89 0.21763907523703807 1101 90-91 0.35587631464535363 1101 92-93 0.48640833567313635 1101 94-95 0.46668181828565025 1101 96-97 0.48370188273139547 1101 98-99 0.28845734448740856 1101 100-101 0.3066404172748385 1102 1 0.4408797993904514 1102 2 0.27447984752973653 1102 3 0.38608666203355213 1102 4 0.33476700117250147 1102 5 0.21191529946548826 1102 6 0.14714150250602387 1102 7 0.3143043878562466 1102 8 0.1910548953855553 1102 9 0.30796773575271885 1102 10-11 0.44994222319649424 1102 12-13 0.42048519944629703 1102 14-15 0.5029868165548379 1102 16-17 0.5869293716538522 1102 18-19 0.5404217746810431 1102 20-21 0.44107892425221706 1102 22-23 0.4708449830785142 1102 24-25 0.4581784908191935 1102 26-27 0.5357074132601625 1102 28-29 0.39794157308499223 1102 30-31 0.4253141347879037 1102 32-33 0.32746589387752323 1102 34-35 0.44788498419706 1102 36-37 0.3540287650240259 1102 38-39 0.4119763558402809 1102 40-41 0.3028279518124606 1102 42-43 0.31619643049354096 1102 44-45 0.38262739446498273 1102 46-47 0.20056651737379383 1102 48-49 0.39066386605106374 1102 50-51 0.34686364545069637 1102 52-53 0.3126283530055005 1102 54-55 0.3986674489370188 1102 56-57 0.6065041474254969 1102 58-59 0.42086766932256836 1102 60-61 0.2448249074215667 1102 62-63 0.2929824408603636 1102 64-65 0.4919795169115133 1102 66-67 0.3618455489225312 1102 68-69 0.13128640091600374 1102 70-71 0.31689889654891346 1102 72-73 0.4821430390056207 1102 74-75 0.38377930900748325 1102 76-77 0.3496345895305737 1102 78-79 0.42815253645939677 1102 80-81 0.5869960574900546 1102 82-83 0.4414948037303539 1102 84-85 0.3068242923441744 1102 86-87 0.24235303711442313 1102 88-89 0.3169610633310995 1102 90-91 0.3803296999061416 1102 92-93 0.5590711402535185 1102 94-95 0.5460474388623737 1102 96-97 0.5796398548258033 1102 98-99 0.5728004705042125 1102 100-101 0.4511324703034738 1103 1 0.2793862425854954 1103 2 0.2039078519538542 1103 3 0.21594469284106665 1103 4 0.1812777532722265 1103 5 0.11515000227780092 1103 6 0.10456874653519321 1103 7 0.18666820803436224 1103 8 0.1447341700148641 1103 9 0.14280505731912996 1103 10-11 0.2816600173022863 1103 12-13 0.28243787350185556 1103 14-15 0.4265984477172111 1103 16-17 0.41869613058561583 1103 18-19 0.38122200395164185 1103 20-21 0.5869615224410936 1103 22-23 0.5132766051391755 1103 24-25 0.4558766039452209 1103 26-27 0.5383387326029947 1103 28-29 0.536759374967545 1103 30-31 0.39219105017668454 1103 32-33 0.4637130423964777 1103 34-35 0.2995779070515354 1103 36-37 0.1918189176214753 1103 38-39 0.2979378162018165 1103 40-41 0.30048574096416303 1103 42-43 0.423219858722625 1103 44-45 0.2845022432500741 1103 46-47 0.3229915435843722 1103 48-49 0.454624710031851 1103 50-51 0.4171575136143417 1103 52-53 0.4276269272649742 1103 54-55 0.5439444674399638 1103 56-57 0.5112265168622301 1103 58-59 0.499732182208966 1103 60-61 0.5700800645698578 1103 62-63 0.5318688655063752 1103 64-65 0.4000091723143484 1103 66-67 0.5003076357458127 1103 68-69 0.514123653355604 1103 70-71 0.3166560526142135 1103 72-73 0.2466855923196718 1103 74-75 0.15842049764303923 1103 76-77 0.3862980830845011 1103 78-79 0.3357551897769042 1103 80-81 0.30259628704867936 1103 82-83 0.42278898008437693 1103 84-85 0.23004204468584533 1103 86-87 0.1783090249365813 1103 88-89 0.38233630671508934 1103 90-91 0.400048483389444 1103 92-93 0.22988655582087958 1103 94-95 0.22695266888184307 1103 96-97 0.38533590422843034 1103 98-99 0.5124567230244601 1103 100-101 0.6286038618838283 1104 1 0.4294998247681079 1104 2 0.29868994837606877 1104 3 0.2776011359651669 1104 4 0.33223336414800286 1104 5 0.3218684284940778 1104 6 0.2654279367205916 1104 7 0.2648083943504034 1104 8 0.20760666455925048 1104 9 0.38626672743949086 1104 10-11 0.46243992918726207 1104 12-13 0.3889138153208833 1104 14-15 0.4216007170685856 1104 16-17 0.4705004750563404 1104 18-19 0.5057219061479472 1104 20-21 0.5267878941379323 1104 22-23 0.3861780281829468 1104 24-25 0.5297114219080896 1104 26-27 0.33193578061238327 1104 28-29 0.4709812319540845 1104 30-31 0.6125079150486599 1104 32-33 0.5103503144680204 1104 34-35 0.5941896684044039 1104 36-37 0.6713260825062832 1104 38-39 0.6051248023503675 1104 40-41 0.5302181217811892 1104 42-43 0.5999018530622351 1104 44-45 0.6030998834302821 1104 46-47 0.6146612666165296 1104 48-49 0.7116410745767894 1104 50-51 0.747490355636927 1104 52-53 0.6279661456300687 1104 54-55 0.7403760270115853 1104 56-57 0.5925659537574504 1104 58-59 0.6871568236972863 1104 60-61 0.8216832237820526 1104 62-63 0.7080904947936872 1104 64-65 0.6682724843142509 1104 66-67 0.4893003264477329 1104 68-69 0.49735566817286525 1104 70-71 0.5723257061140643 1104 72-73 0.6503351983541563 1104 74-75 0.6398933830965667 1104 76-77 0.4202502922254787 1104 78-79 0.528271449641327 1104 80-81 0.6729214805166173 1104 82-83 0.6598447946669062 1104 84-85 0.5289001483333138 1104 86-87 0.7002095073838746 1104 88-89 0.9172422929125403 1104 90-91 0.8604049396559041 1104 92-93 0.8932480497417679 1104 94-95 0.9326038504727414 1104 96-97 0.9656709430137091 1104 98-99 1.1373079162295454 1104 100-101 1.2707551344016004 1105 1 0.2091538263182926 1105 2 0.2154713034629374 1105 3 0.2690187454403379 1105 4 0.23314701321132958 1105 5 0.23247630439181677 1105 6 0.20272533818702954 1105 7 0.17399395203307222 1105 8 0.2914161745169963 1105 9 0.2658609556677263 1105 10-11 0.2929855915409192 1105 12-13 0.1885673360988278 1105 14-15 0.2289196260226447 1105 16-17 0.19258705082473426 1105 18-19 0.08940405186273637 1105 20-21 0.18562090234398454 1105 22-23 0.2644040755745678 1105 24-25 0.2487188332748076 1105 26-27 0.13420385396207024 1105 28-29 0.2453466378460547 1105 30-31 -0.06758249841345076 1105 32-33 0.07463865337771125 1105 34-35 0.06698906982276753 1105 36-37 0.1027531135013362 1105 38-39 -0.02332178415662156 1105 40-41 -0.03925387111469547 1105 42-43 -0.01832105112934812 1105 44-45 0.1399649130876668 1105 46-47 0.06691351286323055 1105 48-49 0.12825486639715677 1105 50-51 -0.13464711734741286 1105 52-53 -0.1055462450624205 1105 54-55 -0.017756245552341454 1105 56-57 0.10942419200191011 1105 58-59 0.01828244281779945 1105 60-61 0.10606929682024457 1105 62-63 0.06422172460705866 1105 64-65 0.07708675654325248 1105 66-67 0.16385266789945518 1105 68-69 0.1604638122324289 1105 70-71 0.10944809032420721 1105 72-73 0.182758134906976 1105 74-75 0.047201100965683196 1105 76-77 0.2685502876399575 1105 78-79 0.10723061409957069 1105 80-81 0.1934978254315105 1105 82-83 0.20921172064757698 1105 84-85 0.14283710939621486 1105 86-87 0.0904199139212487 1105 88-89 0.11479675804433498 1105 90-91 0.1017147288857636 1105 92-93 -0.026388850052978086 1105 94-95 0.1035663585201192 1105 96-97 0.047804820306492246 1105 98-99 0.13358809251539583 1105 100-101 0.22913874176501636 1106 1 0.3570600973644815 1106 2 0.36361568788103327 1106 3 0.18113651250598295 1106 4 0.2652700688327343 1106 5 0.26044061263075236 1106 6 0.3225410773739412 1106 7 0.27645865006766 1106 8 0.4722259482472495 1106 9 0.32816707404751355 1106 10-11 0.5588618487495793 1106 12-13 0.47894702102755815 1106 14-15 0.5580083513865759 1106 16-17 0.7103863997675788 1106 18-19 0.6034185106714816 1106 20-21 0.6246105015176724 1106 22-23 0.4380619975829205 1106 24-25 0.7643510938071998 1106 26-27 0.4817359896400575 1106 28-29 0.5152444009923443 1106 30-31 0.5482731351392687 1106 32-33 0.5014983765757322 1106 34-35 0.522861613061842 1106 36-37 0.448866089203932 1106 38-39 0.4207953821701693 1106 40-41 0.43016268052446094 1106 42-43 0.4644856872437444 1106 44-45 0.4667851432682042 1106 46-47 0.3754813796669296 1106 48-49 0.5250490859543717 1106 50-51 0.3765928821049016 1106 52-53 0.437333630271425 1106 54-55 0.40751370184450053 1106 56-57 0.5461706476854431 1106 58-59 0.48804974636464493 1106 60-61 0.67597942560257 1106 62-63 0.51456107360125 1106 64-65 0.557986876434299 1106 66-67 0.777783391242096 1106 68-69 0.5963445664249747 1106 70-71 0.5608494292359083 1106 72-73 0.6823130361915446 1106 74-75 0.6313623043602838 1106 76-77 0.6494391543743454 1106 78-79 0.628720399399608 1106 80-81 0.6237594891358427 1106 82-83 0.5882613594768742 1106 84-85 0.7070494183813665 1106 86-87 0.63220602949416 1106 88-89 0.7989209715000314 1106 90-91 0.8721778240943117 1106 92-93 0.8394800476767976 1106 94-95 0.9684840863922659 1106 96-97 0.9634588811556917 1106 98-99 0.8807117985093491 1106 100-101 0.8545685438891653 1107 1 0.2320048132755943 1107 2 0.18877797343226987 1107 3 0.24623352716518454 1107 4 0.23101444233917334 1107 5 0.24130340108886372 1107 6 0.18054122004255646 1107 7 0.22381928066295131 1107 8 0.3169427510435554 1107 9 0.295079546851305 1107 10-11 0.42335118427049423 1107 12-13 0.3828788789296098 1107 14-15 0.24166209465440147 1107 16-17 0.4408247490916892 1107 18-19 0.43346095458488065 1107 20-21 0.4861675511306842 1107 22-23 0.2683295904066867 1107 24-25 0.39879709373586536 1107 26-27 0.2155801063429834 1107 28-29 0.3111471366630667 1107 30-31 0.38824219388307313 1107 32-33 0.5466204474106959 1107 34-35 0.6392158949036997 1107 36-37 0.5574213019585059 1107 38-39 0.15214190640600123 1107 40-41 0.37666417854492806 1107 42-43 0.48444368916977254 1107 44-45 0.470230144516556 1107 46-47 0.40685688346547977 1107 48-49 0.5918928994864885 1107 50-51 0.4010333536246833 1107 52-53 0.5173707241117071 1107 54-55 0.5715977693267575 1107 56-57 0.55081361947272 1107 58-59 0.6123961457654374 1107 60-61 0.7517137231379607 1107 62-63 0.684401819758115 1107 64-65 0.6996117828080202 1107 66-67 0.7173873074956205 1107 68-69 0.7044829193158009 1107 70-71 0.5087187626168017 1107 72-73 0.5229294537825808 1107 74-75 0.6089584273406281 1107 76-77 0.590522365844901 1107 78-79 0.706277288510428 1107 80-81 0.644550764949944 1107 82-83 0.7456752232996777 1107 84-85 0.49411383326170366 1107 86-87 0.7455138905349266 1107 88-89 0.6814026029156572 1107 90-91 0.7556135518825933 1107 92-93 0.7783478830372133 1107 94-95 0.7199401979591222 1107 96-97 0.7136733299198248 1107 98-99 0.80158020454423 1107 100-101 0.7855089084074791 1108 1 0.2227050208414667 1108 2 0.19268946809352627 1108 3 0.2127822303459901 1108 4 0.21732023735385297 1108 5 0.2197997640112419 1108 6 0.023964560918074085 1108 7 0.11867314307108501 1108 8 0.17312251056633698 1108 9 0.3237399990083105 1108 10-11 0.2000530841526853 1108 12-13 0.31976927106080666 1108 14-15 0.515404077188947 1108 16-17 0.5308929092352983 1108 18-19 0.3197690870530394 1108 20-21 0.3555207310024002 1108 22-23 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-2.212203552183041 2316 86-87 -2.8518299998514145 2316 88-89 -2.925695330597371 2316 90-91 -2.5619459719269315 2316 92-93 -2.397471973207171 2316 94-95 -2.8478720212681914 2316 96-97 -3.1892523214728072 2316 98-99 -3.1956401458600823 2316 100-101 -3.1348358804514582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5903.0 3 2100.0 4 2117.0 5 2257.0 6 2346.0 7 2656.0 8 3230.0 9 3851.0 10 4653.0 11 5342.0 12 6418.0 13 7685.0 14 8282.0 15 9759.0 16 12068.0 17 15849.0 18 14456.0 19 10270.0 20 10135.0 21 10818.0 22 11823.0 23 12862.0 24 14602.0 25 16786.0 26 19787.0 27 23303.0 28 27678.0 29 33477.0 30 41168.0 31 51444.0 32 65280.0 33 86690.0 34 120740.0 35 174713.0 36 270050.0 37 393270.0 38 327209.0 39 53963.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2751450007191 18.070679790543018 14.388996237862209 23.26517897087567 2 15.59109141142518 19.43359157328952 40.444007145381775 24.531309869903527 3 19.52749630027396 23.828517366293838 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25.38766292137216 28.353784403061187 22.82777694643348 24-25 23.095066794429783 25.649453664116713 28.3874734843782 22.868006057075306 26-27 22.94724583549423 25.82408735016865 28.291448421672538 22.93721839266458 28-29 22.80817224394487 25.84110162353485 28.454106933800876 22.896619198719403 30-31 23.03356285810467 25.67347353470712 28.30285279386302 22.990110813325188 32-33 22.861504548607563 25.752665567591155 28.346625250176732 23.03920463362455 34-35 23.193533172651218 25.82826183721943 28.102516816711503 22.875688173417842 36-37 23.200781765386033 25.68647844561529 28.097611387376258 23.015128401622416 38-39 23.03070204989806 25.797366788988196 28.138390798987544 23.0335403621262 40-41 23.155513267338605 25.678155132620333 28.175730000050397 22.99060159999066 42-43 23.05331334017868 25.82647494387867 27.919575740519054 23.20063597542359 44-45 23.08500897589876 25.909472862229503 27.845603931570974 23.159914230300767 46-47 23.192589804535697 26.018123371871294 27.595132052660325 23.194154770932684 48-49 23.265956783697007 26.15595586860313 27.355100189517266 23.222987158182598 50-51 23.235929639310484 26.3068754830971 27.207312783790538 23.249882093801872 52-53 23.416260096611282 26.339134731918406 27.040894143773148 23.203711027697164 54-55 23.239320800922687 26.255354321602013 27.109605363817995 23.3957195136573 56-57 23.1514563029524 26.444105743631784 27.1226658495399 23.281772103875912 58-59 23.262156917046795 26.284979420593412 27.164498374974933 23.28836528738486 60-61 23.236842126207666 26.09844273257071 27.39545370976869 23.269261431452932 62-63 23.132172854689394 26.258106310653083 27.430009703962817 23.17971113069471 64-65 23.153265115292026 26.208566824279966 27.30330059486309 23.33486746556492 66-67 23.055598369355728 26.463005030039373 27.071512192559698 23.409884408045198 68-69 22.99701791901687 26.33201385977852 27.285934871775826 23.385033349428788 70-71 23.027998833768812 26.273257305459452 27.168597076410162 23.530146784361573 72-73 23.068186882803495 26.348600225390133 27.032623205845713 23.550589685960656 74-75 22.974266082073804 26.526418498559874 26.93772398759398 23.561591431772342 76-77 23.18849192662595 26.427233509675848 26.848651430815192 23.535623132883014 78-79 23.16356072721252 26.45332785671206 26.836134131582725 23.546977284492698 80-81 22.998928360970687 26.589118101307168 26.83403763286374 23.577915904858408 82-83 23.084147980307854 26.500823159366572 26.89787650088949 23.517152359436086 84-85 23.1240847252584 26.501899526710105 26.646008871532565 23.728006876498927 86-87 23.169275764967317 26.41302796368292 26.87049346208764 23.547202809262117 88-89 23.141897538341542 26.263553182062978 26.79933994975341 23.79520932984207 90-91 23.12867726451049 26.330367910828272 26.907110487854187 23.63384433680705 92-93 23.18141886540543 26.33312646708983 26.79280466316666 23.69265000433809 94-95 23.113874065658965 26.297173377933024 26.77071786999693 23.818234686411085 96-97 23.030627068836278 26.244764476049593 26.886811852814947 23.837796602299186 98-99 22.922314905831424 26.354250483528197 26.765780216000696 23.957654394639686 100-101 23.056233239271542 26.258686914369793 26.67750606730408 24.007573779054585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 181.0 1 140.0 2 146.5 3 191.5 4 185.0 5 196.5 6 324.5 7 707.5 8 1291.5 9 1649.5 10 1577.0 11 1469.0 12 1579.0 13 1748.5 14 1893.0 15 2021.5 16 2112.0 17 2258.0 18 2396.0 19 2515.0 20 2764.0 21 3159.0 22 3738.0 23 4562.5 24 5527.0 25 6702.0 26 8494.5 27 10634.5 28 12475.5 29 14601.0 30 17026.0 31 19731.5 32 22690.0 33 25392.0 34 28614.5 35 31523.0 36 34840.0 37 38502.0 38 41953.5 39 44703.0 40 47250.0 41 50782.0 42 54231.0 43 57697.0 44 61400.0 45 65109.5 46 68620.5 47 71931.5 48 75572.0 49 77159.5 50 76155.0 51 75312.5 52 74935.5 53 75102.0 54 74111.0 55 70520.0 56 65881.5 57 59985.0 58 54123.5 59 48495.5 60 41686.0 61 34621.0 62 27656.5 63 21253.5 64 15838.0 65 11485.0 66 7985.5 67 5989.0 68 4301.5 69 2794.0 70 1913.5 71 1185.0 72 711.0 73 424.5 74 259.5 75 154.0 76 95.0 77 53.0 78 29.0 79 22.5 80 18.5 81 14.5 82 14.5 83 12.0 84 7.0 85 6.0 86 4.5 87 4.5 88 5.5 89 5.0 90 3.0 91 2.5 92 1.5 93 3.0 94 3.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040529106423385926 2 0.04090044640370491 3 0.02472063297552074 4 0.04477299191274572 5 0.06408267088933273 6 0.06339303949731177 7 0.06567412794784265 8 0.06763692498667154 9 0.06339303949731177 10-11 0.07166861620156334 12-13 0.0723051990249673 14-15 0.06787564354544803 16-17 0.05469307424412421 18-19 0.047796760323914556 20-21 0.05445435568534772 22-23 0.030078538405837466 24-25 0.017346881937758112 26-27 0.012599035046536856 28-29 0.016949017673130635 30-31 0.01212159792898388 32-33 0.0078246638710071 34-35 0.009760936625527501 36-37 0.005331381146008228 38-39 0.007532896743613616 40-41 0.006180158243880185 42-43 0.004588701185370266 44-45 9.01825666488954E-4 46-47 0.0021484670289883905 48-49 6.631071077124661E-4 50-51 0.0048804683127637504 52-53 0.00469479832260426 54-55 0.007373751037762623 56-57 0.021882534554511383 58-59 0.009071305233506537 60-61 0.020450223201852455 62-63 0.014217016389355273 64-65 0.009416120929517019 66-67 0.01530451204600372 68-69 0.02519807009307371 70-71 0.01917705755504452 72-73 0.0176651733494601 74-75 0.017744746202385597 76-77 0.02519807009307371 78-79 0.036683085198653625 80-81 0.030158111258762957 82-83 0.02957457700397599 84-85 0.020423698917543958 86-87 0.023447467328712803 88-89 0.019097484702119024 90-91 0.018142610467013074 92-93 0.031537374042804885 94-95 0.023765758740414786 96-97 0.0372666194534406 98-99 0.0528894229111463 100-101 0.06349913663454576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1885065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.80526445404718 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16472402112946 47.528267150823915 2 12.39835852140259 15.077709373799477 3 3.9559916561823756 7.216353564965204 4 1.827144737192004 4.444000757631214 5 0.967506404577252 2.9414741395652086 6 0.6087835988231989 2.221034863303871 7 0.4166930187280809 1.7735990439941336 8 0.2972220513167761 1.4458132345512762 9 0.22015563824501072 1.204795962408368 >10 1.0755010861123433 11.48200861066439 >50 0.050597289929966996 2.0848786800217978 >100 0.016534928785210026 1.6052781176010975 >500 4.3724865309081575E-4 0.1550555005429729 >1k 2.623491918544884E-4 0.47883222990036545 >5k 8.744973061816281E-5 0.34089877022667886 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6411 0.3400943734035696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4786 0.253890449400949 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2947 0.15633413171429103 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.304856861699729E-5 0.0 5 0.0 0.0 0.0 5.304856861699729E-5 0.0 6 0.0 1.0609713723399458E-4 0.0 5.304856861699729E-5 0.0 7 5.304856861699729E-5 1.0609713723399458E-4 0.0 5.304856861699729E-5 0.0 8 5.304856861699729E-5 1.0609713723399458E-4 0.0 5.304856861699729E-5 0.0 9 5.304856861699729E-5 1.0609713723399458E-4 0.0 5.304856861699729E-5 0.0 10-11 1.5914570585099188E-4 1.0609713723399458E-4 0.0 5.304856861699729E-5 0.0 12-13 1.5914570585099188E-4 1.3262142154249322E-4 0.0 1.0609713723399458E-4 0.0 14-15 1.8566999015949052E-4 1.5914570585099188E-4 0.0 1.5914570585099188E-4 0.0 16-17 2.1219427446798916E-4 1.5914570585099188E-4 0.0 2.387185587764878E-4 2.6524284308498645E-5 18-19 2.1219427446798916E-4 1.5914570585099188E-4 0.0 2.6524284308498643E-4 1.0609713723399458E-4 20-21 2.1219427446798916E-4 1.5914570585099188E-4 0.0 2.6524284308498643E-4 1.0609713723399458E-4 22-23 2.1219427446798916E-4 1.5914570585099188E-4 0.0 2.917671273934851E-4 1.3262142154249322E-4 24-25 2.6524284308498643E-4 1.5914570585099188E-4 0.0 3.448156960104824E-4 1.5914570585099188E-4 26-27 2.6524284308498643E-4 1.5914570585099188E-4 0.0 5.039614018614743E-4 1.8566999015949052E-4 28-29 2.917671273934851E-4 1.5914570585099188E-4 0.0 7.692042449464607E-4 2.1219427446798916E-4 30-31 3.1829141170198376E-4 1.5914570585099188E-4 0.0 0.0012201170781909378 2.1219427446798916E-4 32-33 3.978642646274797E-4 1.5914570585099188E-4 0.0 0.0020688941760628942 2.6524284308498643E-4 34-35 5.039614018614743E-4 1.5914570585099188E-4 0.0 0.002838098421009355 3.1829141170198376E-4 36-37 5.304856861699729E-4 1.5914570585099188E-4 0.0 0.0037399240874983093 3.1829141170198376E-4 38-39 5.304856861699729E-4 1.5914570585099188E-4 0.0 0.005437478283242222 3.1829141170198376E-4 40-41 5.304856861699729E-4 1.5914570585099188E-4 0.0 0.008381673841485571 3.1829141170198376E-4 42-43 5.835342547869702E-4 1.5914570585099188E-4 0.0 0.010954529419409942 3.1829141170198376E-4 44-45 5.835342547869702E-4 1.5914570585099188E-4 0.0 0.017373406222066613 3.1829141170198376E-4 46-47 7.426799606379621E-4 1.5914570585099188E-4 0.0 0.03315535538562331 3.448156960104824E-4 48-49 7.957285292549594E-4 1.5914570585099188E-4 0.0 0.04437512764811824 3.7133998031898104E-4 50-51 7.957285292549594E-4 1.5914570585099188E-4 0.0 0.05853909546885651 3.7133998031898104E-4 52-53 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.07723871590634807 3.978642646274797E-4 54-55 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.09723802627495604 4.774371175529756E-4 56-57 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.12301963062281673 5.304856861699729E-4 58-59 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.1590130844294494 5.304856861699729E-4 60-61 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.20484704771453505 5.304856861699729E-4 62-63 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.2612642004387117 5.835342547869702E-4 64-65 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.3114746706346996 5.835342547869702E-4 66-67 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.38075610124849807 5.835342547869702E-4 68-69 8.487770978719566E-4 1.5914570585099188E-4 0.0 0.47075299790723396 5.835342547869702E-4 70-71 8.753013821804552E-4 1.5914570585099188E-4 0.0 0.5370637086784806 5.835342547869702E-4 72-73 9.01825666488954E-4 1.5914570585099188E-4 0.0 0.6382803775997115 6.896313920209647E-4 74-75 9.01825666488954E-4 1.5914570585099188E-4 0.0 0.7246699715924915 6.896313920209647E-4 76-77 9.01825666488954E-4 1.5914570585099188E-4 0.0 0.8080357971741028 6.896313920209647E-4 78-79 9.01825666488954E-4 1.5914570585099188E-4 0.0 0.9084036889974616 6.896313920209647E-4 80-81 0.0010609713723399457 1.5914570585099188E-4 0.0 1.0116096792418299 7.161556763294634E-4 82-83 0.0010609713723399457 1.5914570585099188E-4 0.0 1.1427722651473555 7.426799606379621E-4 84-85 0.001114019940956943 1.5914570585099188E-4 0.0 1.324861476925199 7.426799606379621E-4 86-87 0.0011670685095739404 1.5914570585099188E-4 0.0 1.5520950205961066 7.426799606379621E-4 88-89 0.0011670685095739404 1.5914570585099188E-4 0.0 1.8182396893475823 8.487770978719566E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3310 0.0 47.519104 1 GTATCAA 6335 0.0 45.156277 1 ATCAACG 7925 0.0 35.916645 3 TCAACGC 8020 0.0 35.37364 4 TATCAAC 8190 0.0 34.69833 2 CAACGCA 8175 0.0 34.648495 5 AACGCAG 8525 0.0 33.279957 6 ACGCAGA 9610 0.0 29.225832 7 CGCAGAG 9730 0.0 28.57462 8 GCAGAGT 10705 0.0 25.039743 9 GTACATG 7240 0.0 23.89082 1 TACATGG 7435 0.0 22.81805 2 GAGTACT 5520 0.0 22.211489 12-13 AGAGTAC 9260 0.0 21.991758 10-11 AGTACTT 5770 0.0 21.99037 12-13 ACATGGG 7740 0.0 21.242388 3 TGGTATC 1880 0.0 20.474743 2 GTACTTT 5995 0.0 20.370722 14-15 CAGAGTA 10080 0.0 20.084877 10-11 GTGGTAT 1950 0.0 19.982391 1 >>END_MODULE