FastQCFastQC Report
Thu 26 May 2016
SRR936794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885065
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66190.35112847567590505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53300.28274887072859556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.18498035876746954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.046.6934281
GTATCAA69500.039.0406381
ATCAACG89200.030.244973
TATCAAC90350.029.8623812
TCAACGC90500.029.860624
CAACGCA92450.029.230795
AACGCAG96100.028.168496
ACGCAGA110150.024.3599327
CGCAGAG111500.023.8946198
GTACATG76050.022.8065321
TACATGG77200.022.2123342
GCAGAGT121500.021.419859
ACATGGG79750.020.48793
AGAGTAC106200.020.05626310-11
GAGTACT67200.019.3659812-13
AGTACTT69150.019.12895412-13
GTACTTT68550.018.46690214-15
CAGAGTA115950.018.36977410-11
TGGTATC22000.018.3527242
CATGGGG48350.017.582954
Produced by FastQC (version 0.11.5)