FastQCFastQC Report
Thu 26 May 2016
SRR936793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942467
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63130.32499908621356244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47290.24345329933532978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.15130244168884208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.049.89241
GTATCAA67400.042.2263951
ATCAACG83900.033.300813
TCAACGC84000.033.20464
TATCAAC84200.033.1257322
CAACGCA85650.032.5649345
AACGCAG90400.030.7479176
ACGCAGA102550.026.8732747
CGCAGAG104750.025.855278
GTACATG79300.025.044951
TACATGG82100.023.4975382
GCAGAGT112800.023.417979
GTGGTAT19550.022.6023351
TGGTATC18950.022.5669442
GAGTACT57800.021.5347712-13
ACATGGG85950.021.5051843
AGAGTAC99150.020.07727810-11
GTACTTT61250.019.97198114-15
CAGAGTA106200.019.95234310-11
AGTACTT60150.019.74563612-13
Produced by FastQC (version 0.11.5)