FastQCFastQC Report
Thu 26 May 2016
SRR936793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942467
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67240.34615774682401296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.27233409885470383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.1789476989827884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.052.0563241
GTATCAA70800.042.082771
ATCAACG88650.033.216923
TCAACGC89700.032.8810424
TATCAAC90450.032.770142
CAACGCA91650.032.031755
AACGCAG96000.030.5308286
ACGCAGA109400.026.6609557
CGCAGAG111150.026.0275048
GCAGAGT122100.023.2653859
GTACATG80900.022.1443481
TACATGG80000.022.0285662
GAGTACT68000.021.7923112-13
TGGTATC19450.021.7356532
GTACTTT69850.020.94314414-15
GTGGTAT21050.020.7685321
ACATGGG81850.020.5994723
CAGAGTA114500.020.49491710-11
AGAGTAC107700.020.28928810-11
AGTACTT70050.019.90020612-13
Produced by FastQC (version 0.11.5)