FastQCFastQC Report
Thu 26 May 2016
SRR936792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956094
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.3246776484156692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.23659394691666144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29990.15331574045010107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.051.8836021
GTATCAA68150.041.504781
TATCAAC86700.032.4060822
ATCAACG86850.032.2414633
TCAACGC87050.032.112774
CAACGCA88250.031.676115
AACGCAG92000.030.4828516
ACGCAGA103850.026.6786947
CGCAGAG104450.026.2524538
GTACATG80800.024.5930231
GTGGTAT21500.023.2165581
TGGTATC19900.023.172712
TACATGG82750.023.1523552
GCAGAGT115000.022.893629
ACATGGG84800.021.8644223
GAGTACT58650.021.5534212-13
CAGAGTA106800.020.44613510-11
CATGGGG48200.020.417034
GTACTTT62500.020.18796714-15
AGAGTAC100100.019.93940410-11
Produced by FastQC (version 0.11.5)